Dental microwear from Natufian hunter-gatherers and early Neolithic farmers: comparisons within and between samples

Microwear patterns from Natufian hunter-gatherers (12,500-10,250 bp) and early Neolithic (10,250-7,500 bp) farmers from northern Israel are correlated with location on facet nine and related to an archaeologically suggested change in food preparation. Casts of permanent second mandibular molars are examined with a scanning electron microscope at a magnification of 500x. Digitized micrographs are taken from the upper and lower part of facet nine. Microwear patterns are recorded with an image-analysis computer program and compared within and between samples, using univariate and multivariate analyses. Comparisons within samples reveal a greater frequency of pits on the lower part of the facet among the farmers, compared to the upper part. Microwear does not vary over the facet among the hunter-gatherers. Comparisons between samples reveal larger dental pits (length and width) and wider scratches among the farmers at the bottom of the facet, compared to the hunter-gatherers. Microwear does not vary between samples at the top of the facet. The microwear patterns suggest that the Natufian to early Neolithic development led to a harder diet, and this is related to an archaeologically suggested change in food processing. The harder diet of the early farmers may have required higher bite forces that were exerted at the bottom of facet nine, in the basin of the tooth.     

Tooth wear and dental pathology at the advent of agriculture: new evidence from the Levant

Differences in patterns of diet and subsistence through the analysis of dental pathology and tooth wear were studied in skeletal populations of Natufian hunter-gatherers (10,500-8300 BC) and Neolithic populations (8300-5500 BC, noncalibrated) from the southern Levant. 1,160 Natufians and 804 Neolithic teeth were examined for rate of attrition, caries, antemortem tooth loss, calculus, periapical lesions, and periodontal processes. While the Natufian people manifest a higher rate of dental attrition and periodontal disease (36.4% vs. 19%), Neolithic people show a higher rate of calculus. Both populations manifested low and similar rates of caries (6.4% in the Natufian vs. 6.7% in the Neolithic), periapical lesions (not over 1.5%), and antemortem tooth loss (3.7% vs. 4.5%, respectively). Molar wear pattern in the Neolithic is different than in the Natufian. The current study shows that the dental picture obtained from the two populations is multifactorial in nature, and not exclusively of dietary origin, i.e., the higher rate and unique pattern of attrition seen in the Natufian could result from a greater consumption of fibrous plants, the use of pestles and mortars (which introduce large quantities of stone-dust to the food), and/or the use of teeth as a "third hand." The two major conclusions of this study are: 1) The transition from hunting and gathering to a food-producing economy in the Levant did not promote changes in dental health, as previously believed. This generally indicates that the Natufians and Neolithic people of the Levant may have differed in their ecosystem management (i.e., gathering vs. growing grains), but not in the type of food consumed. 2) Changes in food-preparation techniques and nondietary usage of the teeth explain much of the variation in tooth condition in populations before and after the agricultural revolution.     

The Bronze Age burials from Cova Dels Blaus (Vall d′Uixó, Castelló, Spain): An approach to palaeodietary reconstruction through dental pathology, occlusal wear and buccal microwear patterns

This paper reports a palaeodietary investigation of the human remains found in the collective Bronze Age burial cave from Vall d′Uixó (Castelló, Spain). Dental pathology, tooth wear as well as buccal dental microwear were analysed. Percentages of dental pathologies were compared with Chalcolithic and Bronze Age sites from the same territory. Dental caries, ante-mortem tooth loss, periodontal disease and abscess frequencies indicate a diet rich in carbohydrate foods. However, dental calculus percentages and macroscopic wear patterns suggest a diet not exclusively relying on agricultural resources. In addition, buccal dental microwear density and length by orientation recorded on micrographs using a scanning electron microscope showed inter-group differences with regard to carnivorous hunter-gatherers and farming populations related to the amount of abrasives in the diet that could correspond to a different dependence on agricultural resources or food preparation technology.     

牙齿微痕研究在古食性重建中应用的简述

在古食性研究中,牙齿微痕是指动物在咀嚼食物的过程中在牙齿咬合面上产生的微观磨损痕迹。不同食性的动物具有不同的牙齿微痕特征,因此可以通过研究牙齿微痕特征来重建灭绝动物的古食性,为探讨动物演化和古生态环境变化提供重要信息。本文主要介绍牙齿微痕作为一种简单而高效的古食性重建方法在古生物领域中的应用。本文主要内容包括牙齿微痕的发展历史,形成机理与应用,以及近年来被广泛应用的牙齿微痕定量化分析——表面纹理分析法,并在最后浅谈了牙齿微痕研究未来可能研究的方向。     

Paleopathology and the origin of agriculture in the Levant

This study addresses changes in health which were consequential to the Neolithic transition in the southern Levant, judged on the basis of the study of specific and nonspecific stress indicators, trauma, and degenerative joint disease in 200 Natufian (hunter‐gatherer) skeletons (10,500–8300 BC) and 205 Neolithic (agricultural) skeletons (8300–5500 BC) from the southern Levant. The comparison of the health profiles of pre‐Neolithic (Natufian) and Neolithic populations reveals a higher prevalence of lesions indicative of infectious diseases among the Neolithic population, and an overall reduction in the prevalence of skull trauma among males. No change over time was observed in the prevalence of degenerative joint disease. These results indicate that in the southern Levant the Neolithic transition did not simply lead to an overall deterioration in health but rather resulted in a complex health profile which was shaped by 1) an increase exposure to disease agents, 2) changes in diet, 3) population aggregation in larger and denser settlements, 4) changes in activity patterns and the division of labor, and possibly 5) a higher resistant immunological system and response capacity to environmental aggressions (mainly infections). Am J Phys Anthropol 143:121–133, 2010. © 2010 Wiley‐Liss, Inc.     

On the relationship of dental microwear to dental macrowear

Dental microwear analysts have demonstrated that hard diets leave numerous microscopic pits on occlusal surfaces. The relationship between occlusal pitting and gross macrowear, however, is not well known. The current study seeks to elucidate the relationship between dental microwear and macrowear by determining if microscopically pitted teeth are associated with greater expressions of macrowear. This study examined microwear and macrowear on mandibular second molars from 60 prehistoric adult Native Americans representing three dietary regimes (foraging, mixed economy, and agriculture). Initially, two dental microwear feature variables were studied: percentage of pits and mean scratch width. Standard macrowear scores ranged from 4 to 40. ANOVAs suggested that neither of the microwear variables was affected by age or sex, but age affected macrowear scores. Because of this, the sample had a balanced number of young and old adults (i.e., those below and above skeletal age 35). A Pearson's correlation showed no covariation between scratch width and the percentage of pits. Regression analysis indicated that macrowear was not a function of the percentage of pits. However, a significant positive relationship was found between dental macrowear and scratch width. A post priori test found a significant negative relationship between macrowear and the total number of scratches. It is concluded, then, that wide scratches remove more enamel and dentin than do numerous pits, although both cause dental wear. It is suggested here that the term “abrasive” be used to describe those microwear profiles that lead to heavy macrowear and have relatively wide scratches. Am J Phys Anthropol, 2010. © 2009 Wiley‐Liss, Inc.     

F-81 skeleton from Wadi Mataha, Jordan, and its bearing on human variability in the Epipaleolithic of the Levant

The discovery of a Middle Epipaleolithic adult skeleton (F-81) at the site of Wadi Mataha in southern Jordan provides new insights into human variability in the Epipaleolithic of the Levant. This paper analyzes the skeletal morphology of Wadi Mataha F-81 in the context of other Epipaleolithic remains from Jordan and Israel to assess the current evidence for morphological variability throughout this period. The F-81 skeleton shares morphological features with earlier Epipaleolithic skeletons from Ohalo and Nahal Ein Gev, and later Natufian populations. Despite the morphological similarities, F-81 extends the range of known variability prior to the Natufian with its unusually small stature and unique combination of morphological characteristics. High levels of cranial and postcranial robusticity suggest that the F-81 individual was physically active and terrestrially mobile. Pronounced bilateral asymmetry in the upper limb suggests significant lateralization of habitual activity. In the context of Epipaleolithic remains, the F-81 skeleton provides preliminary evidence for greater morphological variability, terrestrial mobility, and lateralized habitual behavior prior to the Natufian, and skeletal gracilization between the Middle and Late Epipaleolithic in the Levant.     

Dental microwear in the orthodentine of the Xenarthra (Mammalia) and its use in reconstructing the palaeodiet of extinct taxa: the case study of Nothrotheriops shastensis (Xenarthra,Tardigrada, Nothrotheriidae)

The utility of orthodentine microwear analysis as a proxy for dietary reconstruction in xenarthrans (tree sloths, armadillos) was quantitatively and statistically accessed via low‐magnification stereomicroscopy. Features such as number of scratches and pits, as well as presence of gouges, hypercoarse scratches, > four large pits, > four cross scratches, and fine, mixed or coarse scratch texture were recorded in 255 teeth from 20 extant xenarthran species. Feature patterns are consistent with scar formation through abrasional (tooth–food) and attritional (tooth–tooth) contact. Number of scratches is the most dietary diagnostic microwear variable for xenarthrans, with herbivorous sloths characterized by > ten scratches and nonherbivorous armadillos by < ten scratches. Discriminant function analysis differentiated arboreal folivores (sloths) and frugivore‐folivores (sloths) both from each other and from fossorial carnivore‐omnivores (armadillos) and insectivores (armadillos). Microwear patterns in carnivore‐omnivores and insectivores are difficult to distinguish between; armadillo microwear may reflect a fossorial lifestyle (grit consumption) rather than primary diet. Cabassous centralis is anomalous in its microwear signal relative to all other insectivores. To test the utility of orthodentine microwear analysis as an indicator of palaeodiet in extinct xenarthrans, microwear in the ground sloth Nothrotheriops shastensis was quantitatively and statistically compared to microwear in extant taxa. Microwear patterns in N. shastensis are most comparable to extant folivores based on scratch number and hierarchical cluster analysis. This strongly supports an herbivorous diet for N. shastensis that is corroborated by multiple independent lines of evidence. Thus, orthodentine microwear analysis can be used to reconstruct diet in extinct xenarthrans. © 2009 The Linnean Society of London, Zoological Journal of the Linnean Society, 2009, 156 , 201–222.     

The pre-Natufian Epipaleolithic: long-term behavioral trends in the Levant

Few cultural developments have taken on as much archeological significance as when people began living in villages and producing their own food. The economic, social, technological, and ideological transformations immediately preceding and following these changes were profound. Early models of culture change associated with pre-agricultural societies of the Levant focused on the sudden, late origin of settled farming villages triggered by climate change. Accompanying this new economic and living situation was durable stone-built architecture; intensified plant and animal use; a flourishing of art and decoration; new mortuary traditions, including marked graves and cemeteries; elaborate ritual and symbolic behavior-a new way of life. This new life style arguably had a slow start, but really took off during the Epipaleolithic period (EP), spanning more than 10,000 years of Levantine prehistory from c. 23,000-11,500 cal BP. The last EP phase, immediately preceding the Neolithic, is by far the best-studied in terms of its cultural and economic contributions to questions on the origins of agriculture.1-4 Recently, archeologists have considered the earlier parts of the EP to be more culturally dynamic and similar to the later phase (Natufian) than was previously thought.3-10 The earlier EP is increasingly seen as demonstrating the behavioral variability and innovations that help us to understand the economic, technological, and social changes associated with complex hunter-gatherers of the Natufian and farmers of the Neolithic. This paper traces the cultural and biological developments of the EP period leading up to the Natufian and considers the long-term trajectory of culture change, social complexity, and village life in the Near East.     

Seasonal Mortality Patterns in Primates: Implications for the Interpretation of Dental Microwear

The microscopic traces of use wear on teeth have been extensively studied to provide information that will assist in elucidating the dietary habits of extinct hominin species. 1 - 13 It has been amply documented that dental microwear provides information pertaining to diet for living animals, where there is a strong and consistent association between dental microwear patterns and different types of foods that are chewed. The details of occlusal surface wear patterns are capable of distinguishing among diets when the constituent food items differ in their fracture properties. 14 - 20 For example, the microwear traces left on the teeth of mammals that crush hard, brittle foods such as nuts are generally dominated by pits, whereas traces left on the teeth of mammals that shear tough items such as leaves tend to be characterized by scratches. These microwear features result from and thus record actual chewing events. As such, microwear patterns are expected to be variably ephemeral, as individual features are worn away and replaced or overprinted by others as the tooth wears down in subsequent bouts of mastication. Indeed, it has been demonstrated, both in the laboratory and the wild, that short‐term dietary variation can result in the turnover of microwear. 17 , 21 - 23 Because occlusal microwear potentially reflects an individual's diet for a short time (days, weeks, or months, depending on the nature of the foods being masticated), tooth surfaces sampled at different times will display differences that relate to temporal (for example, seasonal) differences in diet. 24     

Musculoskeletal stress markers in Natufian hunter-gatherers and Neolithic farmers in the Levant: the upper limb

This paper attempts to quantify the changes in activity patterns of early farming populations in the Levant through the musculoskeletal stress markers (MSM) of the upper limb as seen in skeletal remains. The transition to an agricultural way of life resulted in higher loads on the upper limb in Neolithic populations compared to the Natufian hunter-gatherer populations that preceded them. The MSM pattern for males and females indicates a gender-based division of labor both in the Natufian and the Neolithic. It may also suggest that people in the Neolithic period were engaged in different (new) activities and occupations compared to the Natufian.     

Has the transition to agriculture reshaped the demographic structure of prehistoric populations? New evidence from the Levant

This paper presents the demographic changes that followed the transition from a hunting-gathering way of life (Natufian) to an agricultural, food-producing economy (Neolithic) in the southern Levant. The study is based on 217 Natufian (10,500-8,300 BC) skeletons and 262 Neolithic (8,300-5,500 BC) skeletons. Age and sex identification were carried out, and life tables were constructed. A five-parameter competing hazard model developed by Siler ([1979] Ecology 60:750-757) was used to smooth life-table data. No indication of increased mortality with the advent of agriculture was noted. On the contrary, both life expectancy at birth (24.6 vs. 25.5 years) and adults' mean age at death (31.2 vs. 32.1 years) increased slightly from the Natufian to the Neolithic period (assuming stationary populations). Yet the transition to agriculture affected males and females differently: mean age at death in the Natufian was higher for adult females compared to adult males, while in the Neolithic, it was the reverse. One interpretation given to the distribution of female ages at death is that with the onset of the Neolithic period, maternal mortality increased as a result of a concomitant increase in fertility. If the adoption of agriculture in the Levant increased the rate of population growth at the beginning of the Neolithic, expectation of life may have increased dramatically.     

Brief communication: Short- and long-term in vivo human buccal-dental microwear turnover

Buccal-dental microwear depends on the abrasive content of chewed foodstuffs and can reveal long-term dietary trends in human populations. However, in vivo experimental analyses of buccal microwear formation processes are scarce. Here, we report the effects of an abrasive diet on microwear rates in two adult volunteers at intervals of 8 days over a period of 1 month and document long-term turnover over 5 consecutive years in the same subjects under an ad libitum Mediterranean diet. Buccal microwear was analyzed on mandibular first molars using high-resolution replicas and scanning electron microscopy. Microwear turnover was assessed by recording the scratches lost and gained at each time point. Our results indicate that scratch formation on enamel surfaces increased with a highly abrasive diet compared to both pre-test and post-test ad libitum dietary controls. In the long-term analysis, scratch turnover was higher than expected, but no significant long-term trends in microwear density or length were observed, because microwear formation was compensated by scratch disappearance. Our results confirm that buccal microwear patterns on mandibular molars show a dynamic formation process directly related to the chewing of abrasive particles along with ingested food. In addition, the observed long-term stability of buccal microwear patterns makes them a reliable indicator of overall dietary habits.     

Potential Bark and Fruit Browsing as Revealed by Stereomicrowear Analysis of the Peculiar Clawed Herbivores Known as Chalicotheres (Perissodactyla,Chalicotherioidea)

This low magnification stereomicrowear study samples a broad range of chalicotheres (Perissodactyla, Chalicotherioidea), including basal chalicotheres and the two chalicotheriid subfamilies Schizotheriinae and Chalicotheriinae, primarily including species from North America and Europe, but also some from Asia. The schizotheriines Moropus, Tylocephalonyx, and Metaschizotherium and the chalicotheriines Anisodon and Chalicotherium are best represented. Paleodiets are interpreted via discriminant analysis, using comparison of microwear variables from fossil chalicothere teeth with those from a database of extant ungulates with known diets. The results suggest that all of the chalicotheres in the study were browsers, with no evidence of significant grass consumption. Basal chalicotheres, like basal equids, seem to have been standard fruit-dominated browsers. Stereomicrowear agrees with mesowear results by Schulz et al. (2007) and Schulz and Fahlke (2009) for Metaschizotherium bavaricum, Metaschizotherium fraasi, Anisodon grande, and Chalicotherium goldfussi in showing a highly abrasive aspect to the diet. In these species, hard food objects such as fibrous fruits, seeds, pits, and nuts may have abraded the teeth (based on high pit counts, the presence of large puncture pits, and many individuals with coarse to hypercoarse scratches). Anisodon grande and C. goldfussi, despite their relatively short, brachydont teeth, show the highest degree of abrasion within the studied sample. Moropus and Tylocephalonyx from North America show somewhat different but also abrasive microwear; in these taxa the resistant foods may have been twigs and bark (large pits common, but gouging more prevalent than puncture pits). A preliminary comparison of stereomicrowear on DP4, the deciduous upper fourth premolar, with that on molars suggests that juveniles consumed similar foods as adults but without the most abrasive elements. Some important methodological differences regarding the scoring of microwear features by different low-magnification microwear methodologies are discussed.     

Technical note: An in vitro study of dental microwear formation using the BITE Master II chewing machine

Dental microwear has been used for decades to reconstruct the diets of fossil hominins and bioarchaeological populations. The basic theory has been that hard‐brittle foods (e.g., nuts, bone) require crushing and leave pits as they are pressed between opposing cheek‐tooth surfaces, whereas soft‐tough foods (e.g., grass blades, meat) require shearing and leave scratches as they are dragged along opposing surfaces that slide past one another. However, recent studies have called into question the efficacy of microwear as an indicator of diet. One issue has been the limited number of in vitro studies providing empirical evidence for associations between microwear pattern and chewing behavior. We here describe a new study using a chewing simulator, the BITE Master II, to examine the effects of angle of approach between opposing teeth and food consistency on microwear surface texture. Results indicate that opposing teeth that approach one another: 1) perpendicular to the occlusal plane (crushing) result in pits; 2) parallel to the occlusal plane (shearing) result in striations in the direction of movement; and 3) oblique to the occlusal plane (45°) result in both striations and pits. Results further suggest that different food types and abrasive loads affect the propensity to accumulate microwear features independent of feature shapes. Am J Phys Anthropol 158:769–775, 2015. © 2015 Wiley Periodicals, Inc.     

Dietary Abrasiveness Is Associated with Variability of Microwear and Dental Surface Texture in Rabbits

Dental microwear and 3D surface texture analyses are useful in reconstructing herbivore diets, with scratches usually interpreted as indicators of grass dominated diets and pits as indicators of browse. We conducted feeding experiments with four groups of rabbits (Oryctolagus cuniculus) each fed a different uniform, pelleted diet (lucerne, lucerne & oats, grass & oats, grass). The lowest silica content was measured in the lucerne and the highest in the grass diet. After 25 weeks of exposure to the diets, dental castings were made of the rabbit''s lower molars. Occlusal surfaces were then investigated using dental microwear and 3D areal surface texture analysis. In terms of traditional microwear, we found our hypothesis supported, as the grass group showed a high proportion of (long) “scratches” and the lucerne group a high proportion of “pits”. Regardless of the uniform diets, variability of microwear and surface textures was higher when silica content was low. A high variability in microwear and texture analysis thus need not represent dietary diversity, but can also be related to a uniform, low-abrasion diet. The uniformity or variability of microwear/texture analysis results thus might represent varying degrees of abrasion and attrition rather than a variety of diet items per se.     

Buccal dental microwear variability in extant African Hominoidea: taxonomy versus ecology

Buccal microwear patterns on teeth are good indicators of the abrasiveness of foodstuffs and have been used to trace the dietary habits of fossil species, including primates and hominids. However, few studies have addressed the variability of this microwear. The abrasiveness of dietary components depends not only on the hardness of the particles ingested, but also on the presence of dust and other exogenous elements introduced during food processing. These elements are responsible for the microwear typology observed on the enamel surfaces of primate teeth. Here we analyzed the variability of buccal microwear patterns in African Great Apes (Gorilla gorilla and Pan troglodytes), using tooth molds obtained from the original specimens held in several osteological collections. Our results suggest that ecological adaptations at subspecies or population level account for differences in microwear patterns, which are attributed to habitat and ecological conditions within populations rather than differences between species. The findings from studies on the variability of buccal dental microwear in extant species will contribute to a better understanding of extinct hominids’ diet and ecology.     

Brief communication: intertooth and intrafacet dental microwear variation in an archaeological sample of modern humans from the Jordan Valley

Dental microwear was recorded in a Bronze-Iron Age (3570-3000 BP) sample of modern humans recovered from Tell es-Sa'idiyeh in the Jordan Valley. Microwear patterns were compared between mandibular molars, and between the upper and lower part of facet 9. The comparison revealed a greater frequency of pits and shorter scratches on the second and third molars, compared to the first. Pit frequency also increased on the lower part of the facet on the first molar, compared to the upper part. These results support previous calls for standardization when selecting a molar type for a diet-microwear study. Otherwise the microwear variations along the tooth row could mask any diet-microwear correlations. The results also suggest that there may be a need to choose a consistent location on a facet in order to enhance comparability among studies.     

Upper palaeolithic and late stone age human diet

Undoubtedly modern mankind is an omnivorous species. Nevertheless, types of diet changed at the time of anthropogenesis. The Upper Palaeolithic period is the crucial time because of the appearance of anatomically modern humans in Europe. The main goal in this period investigation is to find the Neanderthal man-Upper Palaeolithic man diet distinction. A sharp early Holocene rise in humidity and temperature and melting of the permafrost resulted in the complete destruction of traditional migration routes, campsite losses, and the flora and fauna of inland territories changing. All these factors affected nutrition patterns, too. The comparison of the different Mesolithic and Neolithic groups' diet patterns allow us to discuss problems of the influence of cultural and ecological factors. The bone tissue chemical concentrations (Ca, Zn, Sr, Cu) from Upper Palaeolithic, Mesolithic and Neolithic burials are considered to reconstruct individual and group patterns of nutrition. The results of the atomic absorption method were used.