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1.
1. Artificial lignins have been produced on potato parenchyma. 2. The methoxyl-free lignin and 4-hydroxy-3-methoxy (guaiacyl) lignins could be estimated by the sulphuric acid method but the 4-hydroxy-3,5-dimethoxy (syringyl) lignins could not. 3. Permanganate oxidation of isolated p-coumaric lignin gave 4-hydroxybenzoic acid, 4-hydroxyisophthalic acid and small amounts of hydroxytrimesic acid and 4-hydroxyphthalic acid. Ferulic lignin gave vanillic acid and 5-carboxyvanillic acid and also small amounts of 4-hydroxybenzoic acid and dehydrodivanillic acid. The sinapic lignin gave traces of syringic acid and of 4-hydroxybenzoic acid. 4. The p-coumaric lignin is a highly condensed polymer. The ferulic lignin is partly uncondensed and partly condensed through the 5-position like gymnosperm lignin. The sinapic lignin shows no evidence of condensation and is probably an ether-linked polymer. 相似文献
2.
Duane C. Ulmer Matti S. A. Leisola Brigitte H. Schmidt Armin Fiechter 《Applied microbiology》1983,45(6):1795-1801
Phanerochaete chrysosporium degraded purified Kraft lignin, alkali-extracted and dioxane-extracted straw lignin, and lignosulfonates at a similar rate, producing small-molecular-weight (~1,000) soluble products which comprised 25 to 35% of the original lignins. At concentrations of 1 g of lignin liter−1, 90 to 100% of the acid-insoluble Kraft, alkali straw, and dioxane straw lignins were degraded by 1 g of fungal mycelium liter−1 within an active ligninolytic period of 2 to 3 days. Cultures with biomass concentrations as low as 0.16 g liter−1 could also completely degrade 1 g of lignin liter−1 during an active period of 6 to 8 days. The absorbance at 280 nm of 2 g of lignosulfonate liter−1 increased during the first 3 days of incubation and decreased to 35% of the original value during the next 7 days. The capacity of 1 g of cells to degrade alkali-extracted straw lignin under optimized conditions was estimated to be as high as 1.0 g day−1. This degradation occurred with a simultaneous glucose consumption rate of 1.0 g day−1. When glucose or cellular energy resources were depleted, lignin degradation ceased. The ability of P. chrysosporium to degrade the various lignins in a similar manner and at very low biomass concentrations indicates that the enzymes responsible for lignin degradation are nonspecific. 相似文献
3.
Kaisa Marjamaa Karolina Toth Paul Andrew Bromann George Szakacs Kristiina Kruus 《Enzyme and microbial technology》2013,52(6-7):358-369
The (hemi)cellulolytic systems of two novel lignocellulolytic Penicillium strains (Penicillium pulvillorum TUB F-2220 and P. cf. simplicissimum TUB F-2378) have been studied. The cultures of the Penicillium strains were characterized by high cellulase and β-glucosidase as well moderate xylanase activities compared to the Trichoderma reesei reference strains QM 6a and RUTC30 (volumetric or per secreted protein, respectively). Comparison of the novel Penicillium and T. reesei secreted enzyme mixtures in the hydrolysis of (ligno)cellulose substrates showed that the F-2220 enzyme mixture gave higher yields in the hydrolysis of crystalline cellulose (Avicel) and similar yields in hydrolysis of pre-treated spruce and wheat straw than enzyme mixture secreted by the T. reesei reference strain. The sensitivity of the Penicillium cellulase complexes to softwood (spruce) and grass (wheat straw) lignins was lignin and temperature dependent: inhibition of cellulose hydrolysis in the presence of wheat straw lignin was minor at 35 °C while at 45 °C by spruce lignin a clear inhibition was observed. The two main proteins in the F-2220 (hemi)cellulase complex were partially purified and identified by peptide sequence similarity as glycosyl hydrolases (cellobiohydrolases) of families 7 and 6. Adsorption of the GH7 enzyme PpCBH1 on cellulose and lignins was studied showing that the lignin adsorption of the enzyme is temperature and pH dependent. The ppcbh1 coding sequence was obtained using PCR cloning and the translated amino acid sequence of PpCBH1 showed up to 82% amino acid sequence identity to known Penicillium cellobiohydrolases. 相似文献
4.
Ralph J Kim H Lu F Grabber JH Leplé JC Berrio-Sierra J Derikvand MM Jouanin L Boerjan W Lapierre C 《The Plant journal : for cell and molecular biology》2008,53(2):368-379
A molecular marker compound, derived from lignin by the thioacidolysis degradative method, for structures produced when ferulic acid is incorporated into lignin in angiosperms (poplar, Arabidopsis, tobacco), has been structurally identified as 1,2,2-trithioethyl ethylguaiacol [1-(4-hydroxy-3-methoxyphenyl)-1,2,2-tris(ethylthio)ethane]. Its truncated side chain and distinctive oxidation state suggest that it derives from ferulic acid that has undergone bis-8-O-4 (cross) coupling during lignification, as validated by model studies. A diagnostic contour for such structures is found in two-dimensional (13)C-(1)H correlated (HSQC) NMR spectra of lignins isolated from cinnamoyl CoA reductase (CCR)-deficient poplar. As low levels of the marker are also released from normal (i.e. non-transgenic) plants in which ferulic acid may be present during lignification, notably in grasses, the marker is only an indicator for CCR deficiency in general, but is a reliable marker in woody angiosperms such as poplar. Its derivation, together with evidence for 4-O-etherified ferulic acid, strongly implies that ferulic acid is incorporated into angiosperm lignins. Its endwise radical coupling reactions suggest that ferulic acid should be considered an authentic lignin precursor. Moreover, ferulic acid incorporation provides a new mechanism for producing branch points in the polymer. The findings sharply contradict those reported in a recent study on CCR-deficient Arabidopsis. 相似文献
5.
Decomposition of Lignocellulose by Cyathus stercoreus (Schw.) de Toni NRRL 6473, a “White Rot” Fungus from Cattle Dung
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Cyathus stercoreus (Schw.) de Toni NRRL 6473, isolated from aged and fragmented cattle dung collected from a Michigan pasture, effected substantial losses in lignin (45%) from wheat straw during a 62-day fermentation (25°C). The basidiomycete also improved wheat straw digestibility by freeing α-cellulose for enzymatic hydrolysis to glucose (230 mg of glucose per 1,000 mg of fermented residue). The rationale for selecting C. stercoreus in attempting to biologically modify the lignin and cellulose components in wheat straw or other gramineous agricultural residues was based on the expectation that this organism is ecologically specialized to enzymatically attack the substructures of native lignins in grasses. 相似文献
6.
Sulfur-free lignins from alkaline pulping tested in mortar for use as mortar additives 总被引:7,自引:0,他引:7
Sulfur-free lignin, obtained through the acid precipitation of black liquor from the soda pulping process, has been tested as water reducer in mortar. It has also been compared to existing commercial additives such as naphthalene sulfonates and lignosulfonates. The ash content and sugar content of these lignins are low in comparison to lignosulfonates, conferring on them higher purity. A procedure for small scale testing derived from the industrial norms SN-EN196 and ASTM (Designation C230-90) is presented. Specifically, all the sulfur-free lignins tested improved the flow of the mortar. Selected flax lignins performed better than lignosulfonates though still less than naphthalene sulfonates. Furthermore, certain hemp lignins gave comparable results to the lignosulfonates. Overall, the straw lignin prepared herein is comparable in performance to commercially available lignins, such as Organocell, Alcell and Curan 100. The plant from which the lignin was isolated, and the process of the pulp mill are the primary influences on the performance of the lignin. 相似文献
7.
Impact of lignins isolated from pretreated lignocelluloses on enzymatic cellulose saccharification 总被引:2,自引:0,他引:2
Lignins were enzymatically isolated from corn stover and wheat straw samples and subjected to hydrothermal or wet oxidation pretreatments for enzyme adsorption experimentations. Lignin contents of the isolates ranged from 26 to 71 % (w/w); cellulose ranged from 3 to 22 % (w/w); xylan from 0.7 to 6 % (w/w) and ash was from 5.8 to 30 % (w/w). ATR-IR analyses indicated significant and similar levels of calcium in all lignin isolates. Commercial cellulase adsorption studies showed that the presence of these lignins had no significant impact on the total amount of adsorbed enzyme in cellulose and cellulose–lignin systems. Consequently, the presence of the lignins had minimal effect, if any, on enzymatic cellulose conversion. Furthermore, this result, coupled with significant calcium levels in the isolated lignins, supports previous work suggesting lignin–calcium complexes reduce enzyme–lignin interactions. 相似文献
8.
Agricultural by-products such as wheat straw are attractive feedstocks for the production of second-generation bioethanol due to their high abundance. However, the presence of lignin in these lignocellulosic materials hinders the enzymatic hydrolysis of cellulose. The purposes of this work are to study the ability of a laccase-mediator system to remove lignin improving saccharification, as a pretreatment of wheat straw, and to analyze the chemical modifications produced in the remaining lignin moiety. Up to 48 % lignin removal from ground wheat straw was attained by pretreatment with Pycnoporus cinnabarinus laccase and 1-hydroxybenzotriazole (HBT) as mediator, followed by alkaline peroxide extraction. The lignin removal directly correlated with increases (~60 %) in glucose yields after enzymatic saccharification. The pretreatment using laccase alone (without mediator) removed up to 18 % of lignin from wheat straw. Substantial lignin removal (37 %) was also produced when the enzyme-mediator pretreatment was not combined with the alkaline peroxide extraction. Two-dimensional nuclear magnetic resonance (2D NMR) analysis of the whole pretreated wheat straw material swollen in dimethylsulfoxide-d 6 revealed modifications of the lignin polymer, including the lower number of aliphatic side chains involved in main β-O-4′ and β-5′ inter-unit linkages per aromatic lignin unit. Simultaneously, the removal of p-hydroxyphenyl, guaiacyl, and syringyl lignin units and of p-coumaric and ferulic acids, as well as a moderate decrease of tricin units, was observed without a substantial change in the wood polysaccharide signals. Especially noteworthy was the formation of Cα-oxidized lignin units during the enzymatic treatment. 相似文献
9.
Heiss-Blanquet S Zheng D Lopes Ferreira N Lapierre C Baumberger S 《Bioresource technology》2011,102(10):5938-5946
The present study aimed to determine the impact of cell wall composition and lignin content on enzyme adsorption and degradability. Thioacidolysis analysis of residual lignins in wheat straw after steam-explosion or organosolv pretreatment revealed an increase in lignin condensation degree of 27% and 33%, respectively. Surface hydrophobicity assessed through wettability tests decreased after the pretreatments (contact angle decrease of 20-50%), but increased with enzymatic conversion (30% maximum contact angle increase) and correlatively to lignin content. Adsorption of the three major cellulases Cel7A, Cel6A and Cel7B from Trichoderma reesei decreased with increasing hydrolysis time, down to 7%, 31% and 70% on the sample with the highest lignin content, respectively. The fraction of unspecifically bound enzymes was dependent both on the enzyme and the lignin content. Adsorption and specific activity were shown to be inversely proportional to lignin content and hydrophobicity, suggesting that lignin is one of the factors restricting enzymatic hydrolysis. 相似文献
10.
《Animal Feed Science and Technology》2002,95(1-2):83-92
Several methods have been developed to protect feed protein from rumen microbial degradation. The current study aimed to evaluate the potential use of an industrial lignin, namely hydrolytic lignin, to protect protein from rumen microbial degradation. The hydrolytic lignins assessed in this study were extracted from wheat straw previously subjected to various steam treatment conditions (pressure: 15, 17 and 19 bar; reaction time: 0, 5 and 10 min; use of acidic catalyst: without and with 2% H2SO4 on DM basis). Results indicated that hydrolytic lignin can precipitate protein when measured by a standard bovine serum albumin assay. It was also observed that protein-precipitating capacity of lignin increased with increasing harshness of steam treatment until a point from which no further effect was observed. The effect of lignin upon protein degradation in vitro was clearly detected. Both ammonia nitrogen and iso-acid concentration in vitro were significantly decreased (P<0.01) when lignin was added to fermentation flask containing casein. Unlike tannins, hydrolytic lignins do not inhibit rumen microbial activity. Additionally, it was observed that lignin’s ability to bind and protect protein is a pH-dependent reaction. Protein binding to lignin is markedly reduced at pH<3.0. 相似文献
11.
Effect of bio-treatment on the lipophilic and hydrophilic extractives of wheat straw 总被引:1,自引:0,他引:1
M.H. Qin Q.H. Xu Z.Y. Shao Y. Gao Y.J. Fu X.M. Lu P.J. Gao B. Holmbom 《Bioresource technology》2009,100(12):3082-3087
Wheat straw, an important papermaking raw material in China, was treated with a white-rot fungus of Phanerochaete chrysosporium ME446, and the lipophilic and hydrophilic extractives from the control and bio-treated samples were analyzed by GC and GC–MS. Bio-treatment of wheat straw could alter the chemical composition of both the lipophylic and hydrophilic extractives. Sugars and phenolic substances such as coniferyl alcohol, 4-hydroxycinnamic acid, 1-guaiacylglycerol and ferulic acid were substantially degraded or consumed by the fungus. More lipophilic substances such as wax, glycerides and steryl esters were degraded into the corresponding components, resulting in much higher concentrations of fatty acids and sterols in the bio-treated samples. Obviously, the bio-treatment of wheat straw was of benefit to pitch control in pulping and papermaking processes, in the view of degradation of the more lipophilic substances. In addition, the bio-treatment could increase the lignin concentration in hot-water extractives of wheat straw. 相似文献
12.
Wei Fang Zemin Fang Peng Zhou Fei Chang Yuzhi Hong Xuecheng Zhang Hui Peng Yazhong Xiao 《PloS one》2012,7(11)
The digestion of lignin and lignin-related phenolic compounds from bamboo by giant pandas has puzzled scientists because of the lack of lignin-degrading genes in the genome of the bamboo-feeding animals. We constructed a 16S rRNA gene library from the microorganisms derived from the giant panda feces to identify the possibility for the presence of potential lignin-degrading bacteria. Phylogenetic analysis showed that the phylotypes of the intestinal bacteria were affiliated with the phyla Proteobacteria (53%) and Firmicutes (47%). Two phylotypes were affiliated with the known lignin-degrading bacterium Pseudomonas putida and the mangrove forest bacteria. To test the hypothesis that microbes in the giant panda gut help degrade lignin, a metagenomic library of the intestinal bacteria was constructed and screened for clones that contained genes encoding laccase, a lignin-degrading related enzyme. A multicopper oxidase gene, designated as lac51, was identified from a metagenomic clone. Sequence analysis and copper content determination indicated that Lac51 is a laccase rather than a metallo-oxidase and may work outside its original host cell because it has a TAT-type signal peptide and a transmembrane segment at its N-terminus. Lac51 oxidizes a variety of lignin-related phenolic compounds, including syringaldazine, 2,6-dimethoxyphenol, ferulic acid, veratryl alcohol, guaiacol, and sinapinic acid at conditions that simulate the physiologic environment in giant panda intestines. Furthermore, in the presence of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), syringic acid, or ferulic acid as mediators, the oxidative ability of Lac51 on lignin was promoted. The absorbance of lignin at 445 nm decreased to 36% for ABTS, 51% for syringic acid, and 51% for ferulic acid after incubation for 10 h. Our findings demonstrate that the intestinal bacteria of giant pandas may facilitate the oxidation of lignin moieties, thereby clarifying the digestion of bamboo lignin by the animal. 相似文献
13.
Nickolas A. Anderson Yuki Tobimatsu Peter N. Ciesielski Eduardo Ximenes John Ralph Bryon S. Donohoe Michael Ladisch Clint Chapple 《The Plant cell》2015,27(8):2195-2209
Modifying lignin composition and structure is a key strategy to increase plant cell wall digestibility for biofuel production. Disruption of the genes encoding both cinnamyl alcohol dehydrogenases (CADs), including CADC and CADD, in Arabidopsis thaliana results in the atypical incorporation of hydroxycinnamaldehydes into lignin. Another strategy to change lignin composition is downregulation or overexpression of ferulate 5-hydroxylase (F5H), which results in lignins enriched in guaiacyl or syringyl units, respectively. Here, we combined these approaches to generate plants enriched in coniferaldehyde-derived lignin units or lignins derived primarily from sinapaldehyde. The cadc cadd and ferulic acid hydroxylase1 (fah1) cadc cadd plants are similar in growth to wild-type plants even though their lignin compositions are drastically altered. In contrast, disruption of CAD in the F5H-overexpressing background results in dwarfism. The dwarfed phenotype observed in these plants does not appear to be related to collapsed xylem, a hallmark of many other lignin-deficient dwarf mutants. cadc cadd, fah1 cadc cadd, and cadd F5H-overexpressing plants have increased enzyme-catalyzed cell wall digestibility. Given that these CAD-deficient plants have similar total lignin contents and only differ in the amounts of hydroxycinnamaldehyde monomer incorporation, these results suggest that hydroxycinnamaldehyde content is a more important determinant of digestibility than lignin content. 相似文献
14.
Guerra A Gaspar AR Contreras S Lucia LA Crestini C Argyropoulos DS 《Phytochemistry》2007,68(20):2570-2583
Despite evidence that lignin associates under both aqueous and organic media, the magnitude and nature of the underlying driving forces are still a matter of discussion. The present paper addresses this issue by examining both solution properties and size exclusion behaviour of lignins isolated from five different species of softwoods, as well as from the angiosperms Eucalyptus globulus and wheat straw. This investigation has used the recently described protocol for isolating enzymatic mild acidolysis lignin (EMAL), which offers lignin samples highly representative of the overall lignin present in the wood cell wall. The molecular weight distributions of these EMALs were found to be dependent upon the wood species from which they were isolated and upon the incubation conditions used prior to size exclusion chromatography. While the chromatograms of EMALs isolated from softwoods displayed a bimodal behaviour, the elution profiles of EMAL from E. globulus and straw were nearly unimodal. A marked tendency to dissociate prevailed under incubation at room temperature for all examined species with the exception of the straw lignin preparation; furthermore, lignin solutions incubated at 4 degrees C showed an associative behaviour manifested by an increase in the weight and number average molecular weights for some species. The extent of such association/dissociation, as well as the time needed for the process to reach completion, was also found to depend upon the wood species, i.e. lignins from softwoods were found to associate/dissociate to a greater extent than lignins from E. globulus and straw. The origin of such effects within the lignin structure is also discussed. 相似文献
15.
Abstract Four major hemoproteins were purified by isoelectric focusing from an extracellular crude enzyme preparation, produced by the white rot fungus Phanerochaete chrysosporium under carbon-limited conditions. Both the crude enzyme and the purified proteins oxidised milled wood lignin, HCl-dioxane-extracted straw lignin and alkali straw lignin in the presence of hydrogen peroxide. The oxidation resulted mainly in further polymerisation of the lignins and was enhanced by addition of veratryl alcohol to the reaction mixture. Alkali straw lignin was also polymerised by horseradish peroxidase, although veratryl alcohol had no influence on this reaction. 相似文献
16.
Maria J. Dinis Rui M.F. Bezerra Fernando Nunes Albino A. Dias Cristina V. Guedes Luís M.M. Ferreira John W. Cone Guilhermina S.M. Marques Ana R.N. Barros Miguel A.M. Rodrigues 《Bioresource technology》2009,100(20):4829-4835
The potential of crude enzyme extracts, obtained from solid state cultivation of four white-rot fungi (Trametes versicolor, Bjerkandera adusta, Ganoderma applanatum and Phlebia rufa), was exploited to modify wheat straw cell wall. At different fermentation times, manganese-dependent peroxidase (MnP), lignin peroxidase (LiP), laccase, carboxymethylcellulase (CMCase), avicelase, xylanase and feruloyl esterase activities were screened and the content of lignin as well as hydroxycinnamic acids in fermented straw were determined. All fungi secreted feruloyl esterase while LiP was only detected in crude extracts from B. adusta. Since no significant differences (P > 0.05) were observed in remaining lignin content of fermented straw, LiP activity was not a limiting factor of enzymatic lignin removal process. The levels of esterified hydroxycinnamic acids degradation were considerably higher than previous reports with lignocellulosic biomass. The data show that P. rufa, may be considered for more specific studies as higher ferulic and p-coumaric acids degradation was observed for earlier incubation times. 相似文献
17.
H. Nadji P.N. Diouf A. Benaboura Y. Bedard B. Riedl T. Stevanovic 《Bioresource technology》2009,100(14):3585-3592
Soda lignin, dioxane lignin and milled lignin were isolated from Alfa grass (Stipatenacissima L.). The physico-chemical characterization of three different lignins: one industrial lignin precipitated from soda spent liquor and two lignin preparations isolated under laboratory conditions from Alfa grass (also know as Esparto grass) was performed. The structures of lignins were studied by three non-destructive (FT-IR, solid state 13C NMR and UV/visible spectroscopy) and two destructive (nitrobenzene oxidation and thermogravimetric analysis) methods. Elemental analysis and the methoxyl content determination were performed in order to determine the C9 formulae for the studied lignins. The total antioxidant capacity of the studied lignins has been determined and compared to commercial antioxidants commonly used in thermoplastic industry. 相似文献
18.
Cyathus stercoreus (Schw.) de Toni NRRL 6473, isolated from aged and fragmented cattle dung collected from a Michigan pasture, effected substantial losses in lignin (45%) from wheat straw during a 62-day fermentation (25 degrees C). The basidiomycete also improved wheat straw digestibility by freeing alpha-cellulose for enzymatic hydrolysis to glucose (230 mg of glucose per 1,000 mg of fermented residue). The rationale for selecting C. stercoreus in attempting to biologically modify the lignin and cellulose components in wheat straw or other gramineous agricultural residues was based on the expectation that this organism is ecologically specialized to enzymatically attack the substructures of native lignins in grasses. 相似文献
19.
A.X. Jin J.L. Ren F. Peng F. Xu G.Y. Zhou R.C. Sun J.F. Kennedy 《Carbohydrate polymers》2009,78(3):609-619
Three organic solvents and one aqueous alkaline solution for fully fractional dissolving hemicelluloses from mild ball-milled cell wall of lignified barley straw and maize stems are described: 90% neutral dioxane, 80% dioxane containing 0.05 M HCl, dimethyl sulfoxide (DMSO), and 8% aqueous KOH. The four successive extractions resulted in dissolution of 94.6% and 96.4% of the original hemicelluloses and 93.7% and 95.3% of the original lignin from barley straw and maize stems, respectively. The structures of the hemicellulosic fractions released during the treatment with the neutral solvents of 90% dioxane and DMSO was found to remain intact, while the extractions with 80% acidic dioxane and 8% KOH under the conditions used resulted in a partial depolymerization of dissolved polysaccharides by cleavage of the glycosidic bonds and saponification of the ester groups in the polymers. The 90% neutral dioxane-soluble hemicellulosic fractions consisted mainly of the more branched arabinoxylans and mixed-linkage glucans such as β-glucans, whereas the hemicellulosic fractions solubilized during the sequential treatments with 80% acidic dioxane, DMSO, and 8% KOH are composed of arabino-(4-O-methyl-d-glucurono) xylans as the major hemicellulosic materials. In addition, the hemicellulosic polymers contained small amounts of ferulic and p-coumaric acids and lignins, revealing that the hemicelluloses removed are mostly unbound to the lignins in the cell walls of cereal straws. This non-degradative cell wall dissolution offers the potential to analyze polysaccharide components for the first time, and improve current hemicellulosic isolation method by using high concentration of aqueous alkali from the delignified cell walls. 相似文献
20.
In order to investigate the importance of the monomeric γ-carbon chemistry in lignin biopolymerization and structure, synthetic lignins (dehydrogenation polymers; DHP) were made from monomers with different degrees of oxidation at the γ-carbon, i.e., carboxylic acid, aldehyde and alcohol. All monomers formed a polymeric material through enzymatic oxidation. The polymers displayed similar sizes by size exclusion chromatography analyses, but also exhibited some physical and chemical differences. The DHP made of coniferaldehyde had poorer solubility properties than the other DHPs, and through contact angle of water measurement on spin-coated surfaces of the polymeric materials, the DHPs made of coniferaldehyde and carboxylic ferulic acid exhibited higher hydrophobicity than the coniferyl alcohol DHP. A structural characterization with 13C NMR revealed major differences between the coniferyl alcohol-based polymer and the coniferaldehyde/ferulic acid polymers, such as the predominance of aliphatic double bonds and the lack of certain benzylic structures in the latter cases. The biological role of the reduction at the γ-carbon during monolignol biosynthesis with regard to lignin polymerization is discussed. 相似文献