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1.
Summary Subpopulations of peripheral blood lymhocytes (PBL) from healthy individuals were separated according to their capacity to form various rosettes and tested for their cytotoxic activity on cell lines of urinary bladder and breast carcinomas. The subpopulation exerting the highest natural cytotoxic activity was characterized by the presence of cell surface Fc-receptors and by the lack of receptors for sheep red blood cells and for C'3 on their surface. Treatment with vibrio cholera neuraminidase (VCN) increased the cytotoxicity of unseparated PBL to a level twice as high as that of untreated PBL. The attachment of T-lymphocytes to tumor monolayers was increased several fold after VCN-treatment, while the attachment of other lymphocyte subpopulations was not. Evidence is presented that the augmentation of the cytotoxicity of PBL following VCN-treatment results from the interaction of VCN-treated T-lymphocytes, attached to target cells, with normal killer cells. It is suggested that augmentation of the activity of killer cells by T-lymphocytes may play a role in antitumor defense mechanisms.Abbreviations CMC
Cell-mediated cytolysis
- E-rosettes
Rosettes formed with sheep red blood cells
- EA-rosettes
Rosettes formed with red blood cells coated with antibody
- EAC'-rosettes
Rosettes formed with red blood cells coated with antibody and complement
- FCS
Heat inactivated fetal calf serum
- PBL
Peripheral blood lymphocytes
- RBC
Red blood cells
- RF-TAL
E-rosette forming, target-attached lymphocytes
- SRBC
Sheep red blood cells
- VCN
Vibrio cholera neuraminidase 相似文献
2.
Thymus lymphocytes of 7--8-week human embryos have nuclei of irregular form with 1--3 distinct nucleoli characterized by absence of compact chromatin or heterchromatin. The electron-dense cytoplasm of these cells contains polysomes and an insignificant number of mitochondria. No receptors to sheep red blood cells and T antigen are revealed on their surface. In 11--12-week human embryos one can observe a decrease in the size of thymus lymphocytes, appearance of heterochromatin in their nuclei and receptors to sheep red blood cells (79%), and T antigen (60%) on the cell surface. Subsequently the quantity of compact chromatin in thymus lymphoid cells increases, and the cells acquire definitive properties and structure. 相似文献
3.
The aim of the present study was to determine whether activation of human T-lymphocytes affects their interaction with sheep red blood cells (SRBC). Less than 3% of the E-rosettes formed by freshly isolated peripheral blood lymphocytes (PBL) and SRBC are stable and do not disintegrate after incubation at 37 degrees C. In contrast, about 30% of PBL kept in culture for 5 days in the presence of mitomycin C-treated allogeneic lymphocytes were found to form stable E-rosettes. Whereas no rosettes were formed by freshly isolated PBL incubated with human red blood cells at 24 degrees C, 15% of the cells recovered from mixed lymphocyte reactions (MLR) formed such rosettes. When responder PBL were maintained in culture in the absence of allogeneic stimuli the proportion of cells forming stable E-rosettes depended on the serum present in the medium. Less than 5% of the responder cells kept in medium containing human serum or in serum-free medium formed stable E-rosettes, whereas 18% of the cells maintained in medium containing fetal calf serum formed stable E-rosettes. The proportion of cells forming stable E-rosettes increased before any increase in DNA synthesis was detectable in MLR. Indeed, a high proportion of cells forming stable E-rosettes appeared in MLR taking place in serum-free medium, without any accompanying increase of DNA synthesis. Depletion of cells forming EAC'-rosettes from responder PBL increased the proportion of cells forming stable E-rosettes in MLR. Exposure of the cells recovered in MLR to specific anti-T sera inhibited the formation of both stable and regular E-rosettes. Exposure of the cells recovered in MLR to anti-Ig serum had no effect on the formation of regular rosettes. Anti-Ig serum strongly inhibited the formation of stable E-rosettes by cells grown in medium containing human serum, but had no effect on the formation of stable E-rossettes by cells grown in either serum-free medium or in serum containing fetal calf serum. It is concluded that activated human T lymphocytes are characterized by their capacity to form stable E-rosettes, resistant to incubation at 37 degrees C, and by their capacity to acquire an immunoglobulin coat, possibly by binding immunoglobulin molecules present in their environment. 相似文献
4.
M.V. Martinez-Toledo T. de la Rubia J. Moreno J. Gonzalez-Lopez 《FEMS microbiology letters》1988,51(2-3):173-176
Abstract In human peripheral blood, a factor which induces gonococcal resistance to complement-mediated killing by fresh human serum is more concentrated in the white blood cells of buffy coat than in red blood cells. Futhermore, the resistance-inducing factor is present in both polymorphonuclear phagocytes and mononuclear cells (monocytes and lymphocytes) separated from the buffy coat by centrifugation on Ficoll-Hypaque gradients. These results imply that inflammatory cells mobilised from the blood to sites of infection carry a host factor which, if it is available to the gonococci, would materially increase their ability to resist a major defence mechanism and hence enhance their capacity to maintain and increase infection. 相似文献
5.
Ala-1 (activated lymphocyte antigen-1) is a murine alloantigen expressed only on activated peripheral T and B lymphocytes. The presence or absence of Ala-1 on specific functional lymphocyte subsets was determined by treating the relevant cell population with anti-Ala-1 and complement, and assaying for residual functional activity. By this method, Ala-1 was shown to be on in vivo primed killer T cells cytotoxic for allogeneic tumor cells. It was also found on helper T cells generated in vivo to sheep red blood cells, and on IgM and IgG plaque-forming cells (PFC) to sheep red blood cells. In contrast, splenic precursors of helper cells and of IgM PFC to sheep red blood cells were completely resistant to treatment with anti-Ala-1 and complement. These findings indicate that effector cells can be distinguished from their nonactivated precursors by their expression of Ala-1. 相似文献
6.
T G Robustova I D Kostishin I D Poniakina A L Sosnovski? A G Kats 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1985,(4):33-35
The action of actinolysate on the capacity of lymphocytes and neutrophils for rosette-formation has been studied in patients with actinomycosis of the maxillofacial area and with chronic nonspecific inflammatory processes, as well as in healthy donors. Actinolysate has been found to have different influence on cell activity. In actinomycosis patients with the low capacity of cells for spontaneous E-rosette-formation with sheep red blood cells (SRBC) actinolysate suppresses their activity still greater, while in cases of the high capacity of cells for spontaneous epsilon-rosette-formation with SRBC this capacity is increased. In chronic nonspecific inflammatory processes in the maxillofacial area and in healthy donors the reverse tendency is observed. 相似文献
7.
The authors studied red cells from men and various animals for their capacity to mind to canine peripherical blood lymphocytes and form spontaneous rosettes. 相似文献
8.
Rosette formation by human thymocytes 总被引:6,自引:0,他引:6
A proportion of lymphocytes in human fetal and post-natal thymus, and in blood, formed rosettes with red blood cells from sheep and pig. The count of rosette-forming cells (RFC) among human thymocytes varied widely, from 2–216 per thousand cells, and was higher in fetal than in post-natal life. The count of RFC among human thymocytes was not reduced by specific rabbit anti-human immunoglobulin sera, indicating that the receptor was not of immunoglobulin character; the reaction was inhibited by antithymocyte serum and metabolic poisons and certain enzymes. The receptor may be equivalent to other “non-specific” glycoprotein hemagglutinins in plants and viruses.The importance of species differences in immunological assays is emphasized. Thus human thymocytes gave high counts of RFC only with red blood cells of sheep and pig; moreover thymus lymphocytes from only man and pig, but not several other species including rodents, were highly reactive with sheep red blood cells. The capacity for rosette formation could be a marker for T cells in human blood. 相似文献
9.
A technique for the simultaneous isolation and cryopreservation of bovine lymphocytes was presented. Whole blood was slowly diluted 1:2 with RPMI-Hepes containing 15% Me2SO to yield final concentrations of 50% whole blood and 7.5% Me2SO. Aliquots were cooled to at least ?80 °C at a rate of 2.5 °C/min and subsequently immersed in liquid nitrogen for storage. Samples were thawed rapidly by agitation in a 37 °C water bath and diluted rapidly with warm RPMI-Hepes. After centrifugation, the lymphocyte pellet was washed and suspended in medium for cell identification and lymphocyte stimulation assays. Few red blood cells, granulocytes, and monocytes survived this freezing and thawing procedure. Recovery of lymphocytes was 69–75%, as compared to a recovery of 58–68% using isolation on density gradients. Only small differences in the numbers of lymphocytes that (i) form spontaneous rosettes with sheep red blood cells and (ii) bind anti-IgG were found between the two isolation procedures. Cell proliferation in response to PPD-B or PHA and the enhancement of amino acid transport in response to PPD-B were the same for lymphocytes isolated by both methods. 相似文献
10.
Yingxia Tan Feng Gong Subo Li Shouping Ji Yanping Lu Hongwei Gao Hua Xu Yangpei Zhang 《Glycoconjugate journal》2010,27(4):427-433
It has been reported that: (1) large variations were found in the number of sialic acid (SA) capped with N-acetyllactosamines (SA-Galβ1-4GlcNAc-R) and α-Gal epitopes (Galα1-3Galβ1-4GlcNAc-R) or uncapped N-acetyllactosamines (Galβ1-4GlcNAc-R) on different mammalian red blood cells, and on nucleated cells originating from a given
tissue in various species; (2) goat, sheep, horse and mouse red blood cells lack α-Gal epitopes, despite the expression of
this epitope on a variety of nucleated cells in these species, including lymphocytes differentiated from the same hematopoietic
origin. In this study, flow cytometry and Western blot analyses of pig red blood cells showed that α-Gal epitopes on pig red
cells developed concomitantly after treatment with neuraminidase, suggesting that the terminal N-acetyllactosaminide glycans were capped with SA-α-Gal epitopes. Whereas, the expression of the α-Gal epitopes on red blood
cells from Sika deer (Cevus nippon hortulorum) were found to be absent even though the epitopes were present on their white blood cells. Thus, these results add new data
not only for the terminal carbohydrate structures on cell surface glycans of various mammalian cells, but also for wide variety
of epitope expression on the cells from different tissues, which might be useful for understanding their unique states resulting
from differentiation and evolution. 相似文献
11.
The Na+-independent uptake of l-alanine has been studied in trout red blood cells, isolated hepatocytes and peripheral blood lymphocytes. The present study shows the existence of two functionally different Na+-independent systems for short chain neutral amino acids in these cells. They are designated as asc systems because of their resemblance to systems described in other cell types. Besides their independence of sodium and a rough similarity in substrate preference, the most important property shared by the two carriers is a lack of trans-stimulation, allowing further differentiation from system L. One of them is an unusually stereospecific carrier present in red blood cells, the other is less restrictive and present in hepatocytes and peripheral blood lymphocytes. Extracellular acid pH increases the incorporation to red blood cells, while it slightly depresses the uptake in the other cells. From the data presented, it is not possible, at first, to classify these carriers as asc
1 or asc
2 systems. Moreover, the system present in red cells resembles that found in the nonerythroid cells, BSC-1, while there is no clear parallelism between the system found in hepatocytes/lymphocytes and any of those described previously.This work was supported in part by a grant from the DGICYT (PB91-0235) of the Spanish Government and by a grant from the CIRIT (AR91-21) of the Generalitat de Catalunya. M.A.G. is a recipient of a fellowship from the Generalitat de Catalunya. We would like to express our sincere thanks to Mrs. Rosa Marsol and Mr. Antonino Clemente (Medi Natural, Generalitat de Catalunya) for their help and logistical assistance and to Mr. Robin Rycroft for his editorial help. J.L. Albi, P. Canals and M.A. Gallardo contributed equally to this study. 相似文献
12.
The distribution of red blood cells in alveolar capillaries is typically nonuniform, as shown by intravital microscopy and in alveolar tissue fixed in situ. To determine the effects of red cell distribution on pulmonary diffusive gas transport, we computed the uptake of CO across a two-dimensional geometric capillary model containing a variable number of red blood cells. Red blood cells are spaced uniformly, randomly, or clustered without overlap within the capillary. Total CO diffusing capacity (DLCO) and membrane diffusing capacity (DmCO) are calculated by a finite-element method. Results show that distribution of red blood cells at a fixed hematocrit greatly affects capillary CO uptake. At any given average capillary red cell density, the uniform distribution of red blood cells yields the highest DmCO and DLCO, whereas the clustered distribution yields the lowest values. Random nonuniform distribution of red blood cells within a single capillary segment reduces diffusive CO uptake by up to 30%. Nonuniform distribution of red blood cells among separate capillary segments can reduce diffusive CO uptake by >50%. This analysis demonstrates that pulmonary microvascular recruitment for gas exchange does not depend solely on the number of patent capillaries or the hematocrit; simple redistribution of red blood cells within capillaries can potentially account for 50% of the observed physiological recruitment of DLCO from rest to exercise. 相似文献
13.
Rosette formation of guinea pig thymocytes (Th) and thymus-derived peripheral blood lymphocytes (TBL) was tested under different experimental conditions. Up to 93% of Th and 38% of TBL showed an affinity to rabbit red blood cells (RRBC). Treatment with metabolic inhibitors like sodium azide and sodium cyanide or freezing and thawing nearly abolished rosette formation by TBL but was ineffective with respect to Th. Heating the cells destroyed rosette-forming capacity of both cell types. These results indicate that spontaneous rosette formation with RRBC by Th does not require the live cell. 相似文献
14.
Ox red blood cells (ORBCs) sensitized with rat IgM antibodies formed antibody-coated red blood cell rosettes (EAR) around 9% of rat spleen cells freshly prepared at 4 °C, while an enhancement of the rosette-forming cell (RFC) frequency to 18–20% was observed after having exposed the spleen cells to a temperature shift from 4 to 37 °C. Although the temperature shift was found to increase the RFC frequency, a shedding of IgM-Fc receptors IgM-FcR into the medium was also detected (IgM-FcR-I). Regeneration of shed receptors within 5 hr has been proved, and the sheading-regeneration cycle could be repeated several times. IgM-Fc receptors detectable after the temperature shift (IgM-FcR-II) are neither shed nor detectable on freshly prepared spleen cells. This latter type of receptor is expressed only after incubating the cells at 37 °C for an optimal period of 8–10 hr. Both type I and II IgM-FcRs were detected on T lymphocytes as well as B lymphocytes; therefore they do not mark subpopulation. Both receptors are sensitive to trypsin and the activity of both requires Ca2+ ions. Shed receptors are able to inhibit EAR formation by cells carrying either IgM-FcR-I or IgM-FcR-II. They are sensitive to higher temperatures and agglutinate ORBCs sensitized by IgM antibodies in the presence of Ca2+ ions. EAR-inhibiting capacity was detected in two fractions obtained by gel chromatography of the supernatant after shedding. The elution volume of one of the active fractions corresponds to 130,000 daltons (D), and that of the other to approximately 50,000 D. 相似文献
15.
P Chollet J Chassagne D Guérin B Sauvezie J M Bidet G Bétail R Plagne 《Revue canadienne de biologie / éditée par l'Université de Montréal》1976,35(1):1-9
Cell electrophoresis enables the separation of the lymphocytes in normal human blood into two principal groups, as a function of their migration speed in relation to 1 mum.sec -1..v-1.cm. In 42 healthy adults, 19,9 % of the lymphocytes have a slower migration, and 80,1 % a faster migration than the reference speed. Two known methods are used for the selection of the lymphocytic populations : spontaneous rosetting with sheep red blood cells, a property of the T lymphocytes, and the adherence to nylon wool columns, which is dominant in the case of B lymphocytes. The cells which do not form spontaneous rosettes, and the cells adhering to nylon wool columns have above all a slow migration. On the contrary, cells which do not adhere to nylon columns have a fast migration. These arguments are in favour of the T nature of the rapid migrating lymphocytes, and of the B nature of the slow migrating lymphocytes. 相似文献
16.
M Vicariot J F Abgrall J L Dutel M C Léglise J Brière 《Revue fran?aise de transfusion et immuno-hématologie》1984,27(1):35-44
42 patients with acute leukaemia, treated with cytotoxic drugs, have been evaluated retrospectively: --group I: 11 patients received packed red blood cells and platelets from single donors; --group II: 6 patients received packed red blood cells and platelets from multiple donors; --group III: 25 patients received packed red blood cells and platelets from single or multiple donors and granulocytes transfusions. There was no difference in age, sex, time of follow up, number of transfusions, in the three groups. The rate of alloimmunization defined as lymphocytotoxicity against more than 20% of a panel of 24 lymphocytes, was 33% (36% group I--33% group II--32% group III). This study shows that platelets from single donors are of no use in preventing or delaying alloimmunization. On the other hand, their major interest is to provide alloimmunized patients with compatible platelets. 相似文献
17.
Physiological roles of the intermediate conductance, Ca2+-activated potassium channel Kcnn4 总被引:1,自引:0,他引:1
Begenisich T Nakamoto T Ovitt CE Nehrke K Brugnara C Alper SL Melvin JE 《The Journal of biological chemistry》2004,279(46):47681-47687
Three broad classes of Ca(2+)-activated potassium channels are defined by their respective single channel conductances, i.e. the small, intermediate, and large conductance channels, often termed the SK, IK, and BK channels, respectively. SK channels are likely encoded by three genes, Kcnn1-3, whereas IK and most BK channels are most likely products of the Kcnn4 and Slo (Kcnma1) genes, respectively. IK channels are prominently expressed in cells of the hematopoietic system and in organs involved in salt and fluid transport, including the colon, lung, and salivary glands. IK channels likely underlie the K(+) permeability in red blood cells that is associated with water loss, which is a contributing factor in the pathophysiology of sickle cell disease. IK channels are also involved in the activation of T lymphocytes. The fluid-secreting acinar cells of the parotid gland express both IK and BK channels, raising questions about their particular respective roles. To test the physiological roles of channels encoded by the Kcnn4 gene, we constructed a mouse deficient in its expression. Kcnn4 null mice were of normal appearance and fertility, their parotid acinar cells expressed no IK channels, and their red blood cells lost K(+) permeability. The volume regulation of T lymphocytes and erythrocytes was severely impaired in Kcnn4 null mice but was normal in parotid acinar cells. Despite the loss of IK channels, activated fluid secretion from parotid glands was normal. These results confirm that IK channels in red blood cells, T lymphocytes, and parotid acinar cells are indeed encoded by the Kcnn4 gene. The role of these channels in water movement and the subsequent volume changes in red blood cells and T lymphocytes is also confirmed. Surprisingly, Kcnn4 channels appear to play no required role in fluid secretion and regulatory volume decrease in the parotid gland. 相似文献
18.
Bioassay for thymic extracts: guinea pig spleen lymphocytes-rabbit red blood cells rosette method 总被引:1,自引:0,他引:1
An assay is described for assessing the potency of thymic extracts by the capacity of guinea pig spleen lymphocytes to form rosettes with rabbit red blood cells. Its sensitivity and reproducibility have been evaluated by statistical analysis of the data obtained testing different batches of the calf thymus extract TP-1. 相似文献
19.
G Angarano A Carlone R Ieva N Luchena G Pastore 《Bollettino della Società italiana di biologia sperimentale》1984,60(5):949-953
To evaluate the behaviour of the Theophylline-sensitive T lymphocytes subpopulation some modifications of the standard procedure are proposed. Lymphoprep purified lymphocytes were counted in a Neubauer hemocytometer after Acridine Orange stain, viability was evaluated by Ethidium Bromide counterstain and monocytes contamination was evaluated by the peroxidase stain. Sheep red blood cells were treated with AET, Theophylline was used at 3 mM (final concentration) and the results compared with untreated lymphocytes; the enumeration of the rosetting lymphocytes was facilitated by adding Acridine Orange prior to the resuspension. The modifications described were able to increase the % of rosetting T lymphocytes, to eliminate differences depending by different lots of sheep red blood cells and to decrease differences depending by subjective evaluation of the rosetting T lymphocytes. 相似文献
20.
Human peripheral blood mononuclear cells (PBMC), stimulated by sheep red blood cells (SRBC), focally proliferate in agar and form colonies of anti-SRBC antibody-secreting cells surrounded by hemolytic areas. Two types of colonies develop: type I (diffuse type), which grows deeply into the agar, and type II (compact type), which grows above the former. Immunochemical and ultrastructural studies show that diffuse colonies contain differentiating lymphoid cells, from small lymphocytes to mature plasma cells. About 50% of cells stain positively in their cytoplasm for IgM and only 1-2% for IgG. Most colonies produce light chains of one class, whereas only a few produce both classes. Many cells resemble monocytes or T lymphocytes in their general morphology and lie in close contact with immunoglobulin-positive cells. Compact colonies contain cells not engaged in antibody production. The culture system described here is the first available antigen-dependent colony assay for human PBMC and may be useful for in vitro studies on the mechanism of human B-cell activation. 相似文献