共查询到20条相似文献,搜索用时 15 毫秒
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Peg3 is an imprinted gene exclusively expressed from the paternal allele. It encodes a C2H2 type zinc-finger protein and is involved in maternal behavior. It is important for TNF-NFkB signaling and p53-mediated apoptosis. To investigate the imprinting mechanism and gene expression of Peg3 and its neighboring gene(s), we used a 120 kb Peg3-containing BAC clone to generate transgenic mice. The BAC clone contains 20 kb of 5 and 80 kb of 3 flanking DNA, and we obtained three transgenic lines. In one of the lines harboring one copy of the transgene, Peg3 was imprinted properly. In the other two lines, Peg3 was expressed upon both maternal and paternal transmission. Imprinted expression was linked to the differential methylation of a region (DMR) upstream of the Peg3 gene. A second, maternally expressed gene, Zim1, present on the transgene was expressed irrespective of parental inheritance in all lines. These data suggest that, similar to other imprinted genes within domains, Peg3 and Zim1 are regulated by one or more elements lying at a distance from the genes. The imprinting of Peg3 seen in one line may reflect the presence of a responder sequence. Concerning the expression of the Peg3 transgene, we detected appropriate expression in the adult brain. However, this was not sufficient to rescue the maternal behavior phenotype seen in Peg3 deficient animals. 相似文献
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Imprinted genes play significant roles in the regulation of fetal growth and development, function of the placenta, and maternal
nurturing behaviour in mammals. At present, few imprinted genes have been reported in pigs compared to human and mouse. In
order to increase understanding of imprinted genes in swine, a polymorphism-based approach was used to assess the imprinting
status of three porcine genes in 12 tissue types, obtained from F1 pigs of reciprocal crosses between Rongchang and Landrace
pure breeds. In contrast to human and mouse homologues, porcine PPP1R9A was not imprinted, and was found to be expressed in all tissues examined. The expression of porcine NAP1L5 was detected in pituitary, liver, spleen, lung, kiduey, stomach, small intestine, skeletal muscle, fat, ovary, and uterus,
but undetectable in heart. Furthermore, porcine NAP1L5 was paternally expressed in the tissues where it’s expression was observed. For PEG3, pigs expressed the paternal allele in skeletal muscle, liver, spleen, kidney, and uterus, but biallele in heart, lung, fat,
stomach, small intestine, and ovary. Our data indicate that tissue distribution of the three gene differs among mammals, and
the imprinting of NAP1L5 and PEG3 is well conserved. 相似文献
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Stuart C Andrews Michelle D Wood Simon J Tunster Sheila C Barton M Azim Surani Rosalind M John 《BMC developmental biology》2007,7(1):53
Background
Cdkn1c encodes an embryonic cyclin-dependant kinase inhibitor that acts to negatively regulate cell proliferation and, in some tissues, to actively direct differentiation. This gene, which is an imprinted gene expressed only from the maternal allele, lies within a complex region on mouse distal chromosome 7, called the IC2 domain, which contains several other imprinted genes. Studies on mouse embryos suggest a key role for genomic imprinting in regulating embryonic growth and this has led to the proposal that imprinting evolved as a consequence of the mismatched contribution of parental resources in mammals. 相似文献10.
Crosses between two recently diverged rodent species of the genus Peromyscus result in dramatic parent-of-origin effects on growth and development. P. maniculatus females crossed with P. polionotus males yield growth-retarded conceptuses, whereas the reciprocal cross results in overgrowth and lethality. These hybrid effects
are particularly pronounced in the placenta. We previously detected linkage to two regions of the genome involved in the overgrowth
effects. One locus, termed Peal, is a paternally expressed autosomal locus mapping to a domain whose house mouse equivalent contains several clusters of
imprinted genes. The other locus, termed Mexl, maps to a gene-poor region of the X chromosome. Here we use an advanced intercross line to verify and narrow the regions
of linkage and identify candidate genes for Mexl and Peal. While we have previously shown that Mexl affects both pre-and postnatal growth, we show here that Peal affects only prenatal growth. Utilizing criteria such as mutant phenotypes and allelic expression, we identify the loci encoding
the homeobox protein Esx1 and the zinc-finger protein Pw1/Peg3 as candidates. Both loci exhibit expression changes in the hybrids.
Maria Loschiavo and Quang K. Nguyen contributed equally to this work. 相似文献
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Calpain-3 is a skeletal muscle-specific protease and participates in the regulation of myogenesis. In this study, we quantified
the expression of calpain-3 (CAPN3) mRNA in a Chinese local chicken breed (Sichuan Mountainous Black-boned chicken [MB]), to discern the tissue and ontogenic
expression pattern. Meanwhile, we compared the CAPN3 mRNA expression pattern in MB chicken at 10 weeks with a commercial meat type chicken line (S01) of the same age to identify
the unique expression pattern under different genetic background. A real time quantitative PCR (qRT-PCR) assay was developed
for an accurate measurement of its expression in various tissues from chickens at different ages (0, 2, 4, 6, 8, 10, and 12 weeks).
Expression of the CAPN3 mRNA was detected in the selected tissues, regardless of age. The breast muscle and leg muscle tissues had a significantly
higher expression than the other tissues from the same individual (P < 0.01). Overall, the CAPN3 mRNA level exhibited a “rise-decline” developmental change in detected tissues except for brain. The S01 chicken had a higher
expression of the CAPN3 mRNA in detected tissues than the MB chicken at 10 weeks. The present expression data of chicken CAPN3 gene may provide some information to shed light on the tissue and ontogenic expression pattern during chicken development. 相似文献
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Dongmei Xi Yiduo He Yongke Sun Xiao Gou Shuli Yang Huaming Mao Weidong Deng 《Molecular biology reports》2011,38(3):1883-1887
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Cloning and ontogenetic expression of the uncoupling protein 1 gene <Emphasis Type="Italic">UCP1</Emphasis> in sheep 总被引:2,自引:0,他引:2
The uncoupling protein 1 (UCP1) is an indicator of brown adipocytes and is involved in the control of body temperature and
regulation of energy balance. It abundantly expresses in newborns and has important functions in adults. However, little information
was known on UCP1 gene expression in young and adolescent sheep. In this study, we cloned and identified the full-length DNA and cDNA sequences
of the ovine UCP1 gene, which were 6659 bp and 1621 bp, respectively, and predicted the location of the gene on chromosome 17. Forty-eight
animals with an equal number of males and females each for both Guangling Large Tail sheep (GLT) and Small Tail sheep Han
(STH) sheep were used to study the ontogenetic expression of UCP1 mRNA in eight adipose tissues by quantitative real-time polymerase chain reaction (PCR). The results showed that the mRNA
was expressed in all tissues studied and at all stages from 2 to 12 months of age. Nevertheless, the mRNA in perirenal fat
was expressed significantly higher than that in other tissues and lower in superficial fat than in deep deposits. The highest
expression was observed in animals at 2 months of age and then decreased gradually with age. Global expression in GLT was
significantly higher than that in STH. Interactions between tissue and breed and age also influenced the mRNA expression significantly.
In addition, the mRNA expression was associated with the single nucleotide polymorphism (SNP) haplotypes detected in the cDNA
of the gene. 相似文献
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