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1.
《Molecular & cellular proteomics : MCP》2019,18(10):2078-2088
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- •Proteomic landscapes of Drosophila somatic and reproductive tissues during aging.
- •Pulsed metabolic labeling determines a decline in protein synthesis with age.
- •Drosophila model of human Parkinson's disease signifies an early-onset decline in protein synthesis.
- •Collapse of proteostasis and mitochondria are early signals for normal aging.
2.
《Molecular & cellular proteomics : MCP》2019,18(10):2003-2017
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- •Identification of the first evolutionary divergent sirtuin ScCobB2 in bacteria.
- •Implementing a global quantitative succinylome between ΔScCobB2 and WT cells.
- •ScCobB2 regulates S. coelicolor protein biosynthesis and carbon metabolism pathways.
- •The divergent sirtuin enzymes are prevalent in other groups of Actinobacteria.
3.
《Molecular & cellular proteomics : MCP》2019,18(1):115-126
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- •Application of Sortase A to label protein N-termini across the whole proteome.
- •Novel gel, proteomic and ELISA-based methods to determine N-myristoylation of proteins in cells, without metabolic labelling.
- •Side by side mass spectrometric quantification of changes in protein N-myristoylation by two complementary methods.
- •Improved Biotin-Neutravidin affinity enrichment protocol.
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5.
《Molecular & cellular proteomics : MCP》2019,18(7):1437-1453
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- •mRNA-seq, miRNA-seq, proteomes of P. fulvidraco, P. vachelli, hybrid Huangyou-1.
- •Predicted miRNA-mRNA-protein pairs were found and validated by qRT-PCR and PRM.
- •Immune, metabolism, digestion, absorption, proliferation, development generate heterosis.
- •High parental gene/protein with low parental miRNAs inherit from the mother or father.
6.
《Molecular & cellular proteomics : MCP》2019,18(11):2191-2206
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- •Quantitative high-throughput glycoanalytical technology as a diagnostic tool for ovarian cancer detection.
- •Multiplexed approach harnessing N-glycan data for six glycoproteins from a single biological sample.
- •Detailed characterization of human serum N-glycans from antibodies IgG, IgM and IgA and acute phase proteins transferrin, haptoglobin and alpha-1-antitrypsin.
- •Structural differences in antibody and acute phase protein glycosylation for mechanistic insights.
7.
《Molecular & cellular proteomics : MCP》2018,17(12):2358-2370
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- •First comparative proteomic analysis of white and brown adipocyte secretomes.
- •100 novel adipokines differentially secreted from white versus brown adipocytes.
- •Functionally enriched protein class changes in white and brown adipocytes.
- •200 novel, NE-responsive adipokines from brown adipocytes.
8.
《Molecular & cellular proteomics : MCP》2019,18(11):2207-2224
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- •Quantitative proteomes and epigenetic regulation of T. gondii.
- •Protein crotonylation and 2-hydroxyisobutylation in phenotypically different T. gondii parasites.
- •Regulation of invasion regulation of T. gondii by protein modification.
- •Lysine crotonylation and 2-hydroxyisobutylation regulated in multiple biological processes in phenotypically different T. gondii parasites.
9.
《Molecular & cellular proteomics : MCP》2019,18(8):1669-1682
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- •Spatiotemporal microproteomics analysis of TBI.
- •Injury site microproteomics reveal distinct phases in 10-day frame post TBI.
- •Uninjured proteomic profile is restored in TBI at 10 days post injury.
- •Substantia nigra protein post 3 days suggest link to Parkinson's disease.
10.
《Molecular & cellular proteomics : MCP》2019,18(4):704-714
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- •The proteomes of L. lactis MG1363 and phage p2 at different stages of infection were characterized.
- •16% (226/1412) of the bacterial proteins detected were unique to infected cultures.
- •A targeted approach using synthetic peptides improved the coverage of phage p2 proteome.
- •By means of proteogenomics, we uncovered a conserved phage protein coded by a previously unannotated gene.
- •Deletion of the bacterial gene llmg_0219 (unknown function) impedes phage p2 infection.
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12.
《The International journal of biochemistry》1993,25(4):479-482
- 1.1. Most of proteins which are rapidly degraded inside eukaryotic cells have been found to contain amino acid sequences (PEST sequences) enriched in proline, acidic residues (glutamic acid and/or aspartic acid) and hydrophilic residues (serine and threonine) (Rogers et al. (1986) Science234, 364–368).
- 2.2. This correlation was tested on nuclear proteins and a close relationship was found between nuclear protein stability and the presence of PEST regions.
- 3.3. Nuclear proteins with structural functions which can be considered as stable components of cell nuclei generally lack PEST sequences.
- 4.4. In contrast, regulatory nuclear factors which have specific and transient functions generally possess at least one PEST sequence.
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14.
《Molecular & cellular proteomics : MCP》2019,18(12):2418-2432
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- •Phosphorylation on Y139 of the sheath protein IglB of Francisella.
- •IglB substitutions Y139A, Y139D or Y139E prevent T6SS formation.
- •Y139F substitution delays but does not abolish phagosomal escape in macrophages.
- •Insight into the role of sheath phosphorylation in T6SS biogenesis.
15.
《Molecular & cellular proteomics : MCP》2019,18(6):1036-1053
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- •Establishment of a flow system allowing multi-omics analysis of S. aureus biofilms.
- •Biofilm proteome profiling (intracellular and ECM) plus metabolic footprint analysis.
- •Virulence factors and ribosomal proteins stabilize the ECM as moonlighting proteins.
- •They act as electrostatic bridges between anionic cell surfaces, eDNA and metabolites.
16.
《Molecular & cellular proteomics : MCP》2019,18(1):41-50
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- •Proteome of mature boar spermatozoa from cauda epididymal and ejaculated sources were analyzed by iTRAQ-based LC-MS/MS.
- •1,723 sperm proteins identified (974 of Sus scrofa taxonomy).
- •1,602 sperm proteins quantified (960 of Sus scrofa taxonomy).
- •32 Sus scrofa sperm proteins were differentially expressed among sperm sources.
- •The proteome of boar spermatozoa is remodelled during ejaculation.
17.
《Comparative biochemistry and physiology. B, Comparative biochemistry》1990,95(4):837-848
- 1.1. Three bis(imidoesters) of different span (ca 9–11 Å) have been used to form intermolecular cross-links between the apoproteins of the lobster carapace carotenoprotein, α-crustacyanin.
- 2.2. Dimethylpimelimidate(DMP) is the most effective of the three reagents in cross-linking the oligomeric α-crustacyanin, giving predominantly dimers between apoproteins from each of the two apoprotein classes. The native dimers, β-crustacyanins, are effectively cross-linked with this reagent.
- 3.3. The stability of DMP cross-linked α-crustacyanin and of the native carotenoprotein to urea treatment and to heating have been compared.
- 4.4. Reagents of longer (sulpho-N-hydroxy-succinimide ester; 18 Å) or shorter (1,5-difluoro-2,4-dinitrobenzene; 5 Å) spans than the bis(imidoesters) are similarly able to form cross-linked dimers with the crustacyanins, but less effectively under the conditions of the reactions.
- 5.5. The results are discussed in relation to the previously presented putative structure of β-crustacyanin (Keen et al. 1990b. Eur. J. Biochem. (submitted); Zagalsky et al., 1990. Comp. Biochem. Physiol.97B, 1–18) and to an alternative subunit interface arrangement of the apoproteins for the dimer.
18.
Domain-specific Quantification of Prion Protein in Cerebrospinal Fluid by Targeted Mass Spectrometry
《Molecular & cellular proteomics : MCP》2019,18(12):2388-2400
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- •Targeted mass spectrometry assay to quantify prion protein (PrP) in spinal fluid.
- •Precise measurement of PrP peptide concentration across protein domains.
- •Peptides are uniformly decreased in symptomatic prion disease patients.
- •Assay applicable to humans and preclinical species for drug development.
19.
《Molecular & cellular proteomics : MCP》2019,18(5):954-967
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- •Two-step cross-linking coupled with affinity purification to facilitate structural analysis of protein complexes.
- •Integrated QXL-MS workflow for studying condition-dependent structural changes of protein complexes.
- •Mechanistic insights on in vivo H2O2-induced conformational dynamics of proteasome complexes.
20.
《Molecular & cellular proteomics : MCP》2018,17(11):2242-2255
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- •BioID and IP-MS were conducted to generate a global ZIKV-host protein interactome
- •Interactome consists of >3000 high confidence ZIKV-host protein interactions
- •Data mining indicates that ZIKV proteins interact with multiple host cell organelles
- •An important role for peroxisomes in ZIKV infection is uncovered