共查询到20条相似文献,搜索用时 15 毫秒
1.
Toxicity of plant essential oils to Tetranychus urticae (Acari: Tetranychidae) and Phytoseiulus persimilis (Acari: Phytoseiidae) 总被引:3,自引:0,他引:3
Fifty-three plant essential oils were tested for their toxicity against eggs and adults of Tetranychus urticae Koch as well as adults of Phytoseiulus persimilis Athias-Henriot, by using a filter paper diffusion bioassay without allowing direct contact. Responses varied according to oil type and dose, and mite species. In a plastic container (4.5 by 9.5 cm) bioassay at 14 x 10(-3) microl/ml air, caraway seed, citronella java, lemon eucalyptus, pennyroyal, and peppermint oils gave > 90% mortality against adult T. urticae, whereas 82 and 81% mortality was observed with sage and spearmint oils, respectively. With the exception of sage oil, the other six essential oils were highly effective against T. urticae eggs at 9.3 x 10(-3) microl/ml air. Against adult P. persimilis, these six test oils caused > 90% mortality at 7.1 x 10(-3) microl/ml air. Particularly peppermint oil at 4.7 x 10(-3) microl/ml air was highly toxic. In an acrylic cage (30 by 30 by 40 cm ) test, lemon eucalyptus, pennyroyal, peppermint, and spearmint oils were highly effective against adult T. urticae at 1.4 x 10(-3) microl/ml air. These results indicate that the mode of delivery of these essential oils was largely a result of action in the vapor phase via the respiratory system. The essential oils described herein merit further study as potential fumigants for T. urticae control. 相似文献
2.
Ashley JL Herbert DA Lewis EE Brewster CC Huckaba R 《Journal of economic entomology》2006,99(1):54-59
Management for twospotted spider mite, Tetranychus urticae Koch, populations in peanut, Arachis hypogaea L., relies on acaricides. The outcomes of acaricide applications are most predictable when complete information on their toxicity and specificity is available. Specifically, the degrees to which acaricides impact different stages of T. urticae and natural enemies combined determine the overall efficacy of an acaricide application. The objectives of this study were to determine stage-specific direct and residual efficacies of three acaricides (fenpropathrin, etoxazole, and propargite) against T. urticae, and the direct and residual toxicity of the acaricides to Orius insidiosus (Say) adults. Direct toxicity of acaricides to T. urticae was measured on peanut cuttings. All acaricide treatments caused significant mortality to a mixed stage population of T. urticae, and mortality did not differ among the acaricides 7 d after treatment. When toxicity to eggs was tested, the proportion of eggs that hatched for all acaricide treatments was significantly lower than the control, with etoxazole and propargite causing 100% mortality. Exposure to acaricide residues caused < 30% mortality of T. urticae adults 1 and 2 d after treatment and was not significantly different from the control. Fenpropathrin and propargite caused 100% mortality and etoxazole caused > 50% mortality of O. insidious adults after direct exposure to the acaricides. Residual toxicity of acaricides to O. insidiosus adults varied but remained toxic to O. insidiosus longer than to T. urticae. Fenpropathrin had the longest residual effect on O. insidiosus adults, causing > 95% mortality after 14 d; etoxazole and propargite caused < 30% mortality after 14 d. 相似文献
3.
We investigated the effects of changes in vapor pressure deficit (VPD) on the survival of diapausing (winter form) and non-diapausing (summer form) spider mites Tetranychus urticae Koch and Tetranychus kanzawai Kishida (Acari: Tetranychidae). Adult females of both species were kept without food at VPDs of 0.0, 0.4, 0.7, 1.5, 1.9, or 2.7 kPa for 3, 6, 9, 12, or 15 days at 25 °C. Diapausing females of both species kept at a VPD of ≥0.4 kPa for ≥6 days clearly tolerated desiccation. Under water-saturated conditions (VPD = 0.0 kPa), in which no desiccation occurred, diapausing females showed high starvation tolerance: 90 % survived for up to 15 days. No interspecific differences in tolerance to desiccation or starvation were observed under most conditions. These results indicate that diapause functions increase tolerance to desiccation and starvation. Such multiple tolerances to harsh environments might support winter survival in spider mites. 相似文献
4.
5.
Abstract: Tetranychus urticae is a serious pest of several crops worldwide. It performs differentially on diverse host‐plant species. Because dispersion is mainly passive, the process of host‐plant selection should be viewed in terms of host‐plant acceptance and not in terms of host finding. The objectives of this study were to investigate: (1) the acceptance of strawberry (S) in comparison with onion (O), leek (L) and parsley (P) by T. urticae; and (2) the suitability of these host plants for the performance of T. urticae, in terms of fecundity and survival. Host‐plant acceptance was measured through females that settled on a test plant or females that left for another host plant. In another experiment, for a duration of 5 days, individual newly mated females were assigned to discs of each host plant and the fecundity, the maximum number of offspring obtained from one female that settled on the disc, the survival and the percentage of females that oviposited at least one egg were analysed. A choice test to measure the preference of females for different host plants was performed. Movements took place towards (S) from the three plants: (O), (L) and (P). Mites significantly moved to other places in the Petri dishes from leek‐origin discs. Dispersal of T. urticae from (S) to other host‐plant discs was similar for all plants. Mean fecundity was higher on (S) than on the other plants. The maximum number of offspring per female was highest on (S), lowest on (L) and (O), and intermediate on (P). Survival of females and percentage of females that laid at least one egg were independent of the host plant. The choice test showed that oviposition was higher on (S) and (P) and very poor on (L) and (O) and that more females settled on (S) and (P). Although mites migrated from parsley to strawberry, the first host plant has a higher acceptance of T. urticae in terms of maximum number of offspring per female than onion and leek, and a lower performance in terms of fecundity than strawberry. Parsley could be a good candidate for an associate plant in a strawberry crop; however, it needs more research under field conditions. 相似文献
6.
ANASTASIA TSAGKARAKOU MARIA NAVAJAS PAGONA PAPAIOANNOU-SOULIOTIS & NICOLE PASTEUR† 《Molecular ecology》1998,7(1):71-79
Polymorphism of four enzymatic loci has been examined in 27 populations of Tetranychus urticae in relation to their geographical distribution and to two ecological parameters: open field vs. greenhouse habitats, and species of the colonized host plant. Genetic differentiation was significantly correlated to geographical distance in both types of habitat. Mite density and distribution of infested plants appear to be important factors for the population structure of T. urticae . In open field, T. urticae specimens from citrus trees were genetically more similar to other 'citrus' samples collected in different localities than they were from mites collected in the same locality on other plant species. 相似文献
7.
8.
Tetranychus urticae Koch (Acari: Tetranychidae) and Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae) are major pests in greenhouse crops. Recently, Amblyseius swirskii Athias-Henriot (Acari: Phytoseiidae) was shown to be an effective biological control agent of both pests. Therefore, the prey preference of A. swirskii was determined using immature stages of T. urticae and B. tabaci in three various treatments based on Manly's β preference index (β). These treatments consisted of immature stages of two prey species (egg, first and second instar nymphs) with densities 12:12, 6:6 and 3:3, respectively, and with 13 replicates. After 24?h starvation, same-aged females of A. swirskii were added to the leaf discs. All experiments were done on bean leaf discs in Petri dishes (8?cm in diameter) in laboratory conditions with 25?±?2°C, 70?±?5% relative humidity and the photoperiod of 16L:8D hours. Comparing the preference indices using t-tests indicates a significant preference of the predator on eggs (t?=?10.80, df?=?24, P?0.0001) and second instar nymphs (t?=?8.17, df?=?24, P?0.0001) of T. urticae than B. tabaci. Our findings suggest that developmental stages of prey have effect on the prey selection by A. swirskii. 相似文献
9.
Mechanisms of resistance to organophosphates in Tetranychus urticae (Acari: Tetranychidae) from Greece 总被引:1,自引:0,他引:1
Tsagkarakou A Pasteur N Cuany A Chevillon C Navajas M 《Insect biochemistry and molecular biology》2002,32(4):417-424
We investigated the mechanisms conferring resistance to methyl-parathion (44-fold) and to methomyl (8-fold) in Tetranychus urticae from Greece by studying the effect of synergists on the resistance and the kinetic characteristics of various enzymes in a resistant strain (RLAB) and a susceptible reference strain (SAMB). It is shown that S,S,S-tributyl phosphorotrithioate, a synergist that inhibits esterases and glutathione S-transferases, and piperonyl butoxide, a synergist that inhibits cytochrome P450 mediated monooxygenases, did not affect the level of methyl-parathion or methomyl resistance in RLAB and that resistance ratios to both insecticides did not change significantly in the presence of either synergist. Isoelectric focusing of esterase allozymes on single mites revealed no differences in staining intensity and glutathione S-transferase activity was not significantly different in the two strains. The activity of two cytochrome P450 monooxygenase groups was compared. No significant difference of 7-ethoxyresorufin-O-diethylase activity was observed between strains that were two-fold higher in RLAB than in SAMB. The kinetic characteristics of acetylcholinesterase, the target enzyme of organophosphates and carbamates, revealed that acetylcholinesterase in RLAB was less sensitive to inhibition by paraoxon and methomyl in comparison with SAMB. I(50), the inhibitor concentration inducing 50% decrease of acetylcholinesterase activity was greater (119- and 50-fold with paraoxon and methomyl, respectively) and the bimolecular constant k(i) was lower (39- and 47-fold with paraoxon and methomyl, respectively) in RLAB compared to SAMB. 相似文献
10.
11.
Marjan Seiedy Alireza Saboori Azadeh Zahedi-Golpayegani 《Experimental & applied acarology》2013,60(2):219-227
Determination of attraction and avoidance behavior of predators is important in concomitant use of multiple natural enemies to control a pest. The olfactory response of the predatory mite Phytoseiulus persimilis was studied to odors related to Tetranychus urticae adults infected by Beauveria bassiana DEBI008 in 0, 24, 48 and 72 h intervals, both in absence and in presence of plants. In plant-present experiments, P. persimilis attraction was neither towards adults of T. urticae infected by 0.02 % Tween 80 (as control), nor to the ones infected by B. bassiana for 0 or 24 h, whereas significant attraction towards the control was observed when tested against T. urticae infected by B. bassiana for 48 or 72 h. In absence of plants, P. persimilis displayed significant avoidance of T. urticae infected by B. bassiana for 48 or 72 h, when their alternative option was 0.02 % Tween 80-infected T. urticae adults. These results indicate that P. persimilis can recognize the presence of B. bassiana and that the predator avoids the fungus. This suggests that the two natural enemy species can be used together in biological control programmes. 相似文献
12.
Functional response of Neoseiulus californicus (Acari: Phytoseiidae) to Tetranychus urticae (Acari: Tetranychidae) on strawberry leaves 总被引:2,自引:0,他引:2
Neoseiulus californicus (McGregor) is a predatory mite employed for biological control of the agricultural pest Tetranychus urticae (Koch). We explored whether environmental differences, in this case the trichome densities of abaxial leaf surfaces of strawberry cultivars (‘Maehyang’ and ‘Sulhyang’ varieties) affect the functional response of adult female N. californicus preying on immature stages (egg, larva and nymph) of T. urticae. We also evaluated the functional response of N. californicus to eggs of T. urticae at different temperatures (15, 20, 25, 30 and 35°C). We conducted a logistic regression of the proportion of prey consumed as a function of initial prey density to identify functional response types, and used nonlinear least‐squares regression and the random predator equation to estimate attack rates and handling times. The functional response of adult female N. californicus to T. urticae was not influenced by non‐glandular trichomes on abaxial leaves but was affected by temperature. Overall, adult female N. californicus exhibited a type 2 functional response to T. urticae. The handling time of N. californicus was highest (1.9970 h) against T. urticae nymphs. The attack rate did not change much at 15–30°C, but was significantly higher at 35°C. The handling time decreased significantly with increasing temperature at 15–35°C. At 35°C, the attack rate was highest (0.2087) and the handling time was lowest (0.9511 h). 相似文献
13.
Two species of spider mite occur in greenhouses in the Netherlands.
Tetranychus urticae Koch is usually green, can live on many plants, but cannot build up large populations on carnations; it may have a diapausing stage which is very resistant to cold. No biological races of T. urticae were found.
T. cinnabarinus Boisd. is carmine-coloured, often found on carnations, and does not have a diapause. It is not resistant to cold.The two species do not interbreed. Growers of carnations have only T. cinnabarinus to deal with, and can arrange control measures accordingly.
Zusammenfassung Untersuchungen der Morphologie, der Wirtspflanzenwahl, der Überwinterungsweise und Kreuzingsexperimente haben gezeigt, daß in Gewächshäusern Hollands zwei Spinnmilben-Arten auftreten, nämlich Tetranychus urticae Koch und T. cinnabarinus Boids. Darüberhinaus wurde Material von Spinnmilben je einer Lokalität in Deutschland, der Schweiz und Belgien verwendet. T. urticae ist meistens grün gefärbt, lebt an einem großen Wirtspflanzenkreis, kann aber an Nelken (Dianthus caryophyllus L.) keine Populationen bilden. Diese Art geht unter dem Einfluß verschiedener biologischer Faktoren in Diapause. Während dieser Periode ist sie sehr kälteresistent. Der Winter wird an geschützten Orten verbracht. Kreuzungen zwischen Populationen verschiedener Herkunft ergaben stets eine normale Nachkommenschaft. Zucht-experimente mit diesen Populationen auf verschiedenen Wirtspflanzen ergaben keinen Hinweis für das Bestehen von biologischen Rassen bei T. urticae. T. cinnabarinus ist karminrot gefärbt und wird am häufigsten auf Nelken gefunden, obwohl in Laboratoriumsversuchen die Entwicklung an Buschbohnen (Phaseolus vulgaris L.) schneller verläuft. Diese Art tritt überhaupt nicht in Diapause ein, bleibt den Winter über an den Blättern und ist gegenüber Kältebedingungen entschieden weniger resistent als T. urticae. Kreuzungen zwischen Populationen verschiedener Herkunft ergaben immer eine normale Nachkommenschaft.Das Ausbleiben von Bastardierungen, das in Kreuzungsexperimenten zwischen den beiden Arten gefunden wurde, erbrachte den strengsten Nachweis, daß in den Gewächshäusern zwei verschiedene Arten vorkommen.Da T. cinnabarinus hauptsächlich auf Gewächshäuser mit Nelken beschränkt ist und T. urticae sich andererseits an diesen Pflanzen nicht vermehrt, haben es die Nelkenanbauer lediglich mit T. cinnabarinus zu tun, und es ergibt sich eine Möglichkeit, Nelken frei von Spinnmilbenbefall zu halten.相似文献
14.
Laboratory bioassays were conducted to characterize the activity of the insecticide spinosad against the twospotted spider mite, Tetranychus urticae Koch, and European red mite, Panonychus ulmi (Koch) (Acari: Tetranychidae). T. urticae females and larvae were individually placed on bean, Phaseolus vulgaris L. (Fabaceae), leaf disks treated with four rates of spinosad (25, 55, 121, and 266 ppm) and a water control. Significantly fewer T. urticae completed development on any spinosad rates (<15%) compared with the control (>85%), whereas spinosad exhibited no significant effects on P. ulmi development; 72.5 and 83.1% of P. ulmi completed development on apple (Malus pumila P. Mill, Rosaceae) leaf disks treated with 75 ppm spinosad and the control, respectively. T. urticae adult females placed on spinosad-treated disks had significantly higher mortality and lower oviposition rates compared with the water control; no significant mortality effects were observed until 3 d after placing adults on leaf disks. In choice tests where half of a bean leaf was treated with 55 ppm spinosad transversally or longitudinally, T. urticae females were repelled by spinosad and largely oviposited and fed on nonspinosad treated areas. Spinosad did not affect the behavior of P. ulmi females. When T. urticae females were released on potted bean plants (two-leaf stage) in which leaves received spinosad sprays on the adaxial or abaxial leaf surfaces, or complete spinosad coverage on one or two of the leaves, mite population increase lagged significantly behind those released on control plants. These results indicate that spinosad has significant acaricidal effects against T. urticae but not P. ulmi. 相似文献
15.
Competitive interactions between Tetranychus urticae and Eotetranychus carpini borealis were investigated under laboratory and greenhouse conditions. When placed first to colonize red raspberry leaves, T. urticae caused extinction of E. carpini borealis populations. E. carpini borealis had detrimental effects on T. urticae but did not cause T. urticae population extinction. The area used by E. carpini borealis for egg deposition decreased with an increase in the number of T. urticae eggs. Red raspberry showed an induced response to T. urticae and E. carpini borealis feeding after two weeks of infestation. Population growth of each species was reduced on plants that were previously infested by conspecifics but inducible response by one species had no effects on the other species. 相似文献
16.
Representative fungicides from three or four families used for management of powdery mildew and other diseases in tree fruits were evaluated for their effects on a common spider mite and predator mite species, respectively. A modified Munger cell technique was effective in measuring the response of phytophagous and predaceous mites to fungicide residues on detached leaves in the laboratory. Demethylation-inhibiting (DMI) (imidazole [triflumazole] and triazole [myclobutanil]) and strobilurin (trifloxystrobin) fungicides were not toxic to female Tetranychus urticae Koch and Galendromus occidentalis (Nesbitt), and no sublethal effects were found on fecundity and predation rate after 3-5-d exposure to residues. Benomyl, a benzimidazole fungicide, increased adult mortality and reduced fecundity for both mite species; however, it did not alter the predation rate of G. occidentalis females on T. urticae eggs and larvae. Female G. occidentalis that survived the lethal effects of benomyl and the comparison acaricide pyridaben were unimpaired in predation. Our results for benomyl substantiate those of earlier studies and provide evidence for nontoxic effects of DMI and strobilurin fungicides on mites. We propose that DMI and strobilurin fungicides are a good fit for integrated mite management programs due to conservation of phytoseiid predatory mites. 相似文献
17.
18.
Thirty-four essential oils were screened for their repellent activities against the twospotted spider mite, Tetranychus urticae Koch (Acarina: Tetranychidae), at 0.1% concentration level using choice and no-choice laboratory bioassays. Of these, 20 essential oils showed significant repellencies against T. urticae in the choice tests. In subsequent no-choice tests using these 20 essential oils, only sandalwood oil showed significant repellency against T. urticae. Total number of eggs oviposited by T. urticae was significantly lower than controls in the choice tests when the kidney bean leaves were treated with 1 of 14 essential oils. The significant repellency of sandalwood oil against T. urticae lasted at least for 5 h at the 0.1% concentration level. Our GC-MS analysis indicated that the major components of the sandalwood oil were alpha-santalol (45.8%), beta-santalol (20.6%), beta-sinensal (9.4%), and epi-beta-santalol (3.3%). Santanol, a mixture of the two main components in the sandalwood oil, appears to be responsible for the repellency of sandalwood oil against T. urticae. 相似文献
19.
Aksoy HM Ozman-Sullivan SK Ocal H Celik N Sullivan GT 《Experimental & applied acarology》2008,46(1-4):223-230
This study investigated Pseudomonas putida biotype B as a potential biological control agent of Tetranychus urticae. The bacteria were isolated from greenhouse soil from Carsamba, Turkey. The experiment was carried out in a completely randomized plot design under laboratory conditions. For this purpose, spraying and dipping applications of a suspension of P. putida biotype B (10(8)-10(9) colony forming units/ml) were applied to newly emerged, copulated females. Dead mite and egg counts were started on the 3rd day after treatments, and observations were continued daily until all the mites had died and egg hatching had finished. Both types of bacterial application significantly reduced total egg numbers and egg hatching, compared to their respective controls. Bacterial spraying was significantly more effective than dipping-the spray application demonstrated 100% efficacy and resulted in the fewest viable eggs. The results of this study indicated that P. putida biotype B has a strong efficacy in causing mortality in T. urticae. 相似文献
20.
Multiple resistance and biochemical mechanisms of pyridaben resistance in Tetranychus urticae (Acari: Tetranychidae) 总被引:3,自引:0,他引:3
A field colony of Tetranychus urticae (Koch) (Acari: Tetranychidae) resistant to pyridaben was selected with pyridaben successively for 20 generations to produce the PR-20 strain. Resistance and multiple resistance levels of the PR-20 strain to 15 acaricides were determined using a spray bioassay. The PR-20 strain was extremely resistant to pyridaben (resistance ratio [RR] = 240]. The strain exhibited extremely strong resistance to fenpyroximate (RR=373) and acrinathrin (RR=329) and strong resistance to benzoximate (RR=84). An RR = 10-40 was observed with abamectin, fenazaquin, fenbutatin oxide, fenpropathrin, and tebufenpyrad. The PR-20 strain showed low levels of resistance (RR <10) to azocyclotin, bromopropylate, chlorfenapyr, dicofol, milbemectin, and propargite. Synergist experiments with different metabolic inhibitors revealed that piperonyl butoxide (PBO), a mixed function oxidase (MFO) inhibitor, had the greatest effect on pyridaben resistance. PBO significantly caused pyridaben resistance in the PR-20 strain to drop to the full susceptibility level of the susceptible (S) strain. However, there was no significant difference in MFO activities measured using a model substrate between the S and PR-20 strains. These results suggest that use of certain acaricides with little multiple resistance or PBO will be useful for the management of pyridaben resistance in the field. 相似文献