共查询到20条相似文献,搜索用时 0 毫秒
1.
The problem of dilute solvent concentration in butanol-acetone fermentations can be solved by using reverse osmosis to dewater the fermentation liquor. Polyamide membranes have a potential application in a butanol-acetone fermentation and exhibited rejection rates as high as 98%. Optimum rejection of butanol in the fermentation liquor occurred at recoveries of 20-45%. Flux ranged from 0.05 to 0.6 L m(-2) min(-1). 相似文献
2.
Lin X Wu J Jin X Fan J Li R Wen Q Qian W Liu D Chen X Chen Y Xie J Bai J Ying H 《Biotechnology progress》2012,28(4):962-972
The traditional distillation method for recovery of butanol from fermentation broth is an energy-intensive process. Separation of butanol based on adsorption methodology has advantages in terms of biocompatibility and stability, as well as economy, and therefore gains much attention. However, the application of the commercial adsorbents in the integrated acetone-butanol-ethanol (ABE) fermentation process is restricted due to the low recovery (less than 85%) and the weak capability of enrichment in the eluent (3-4 times). In this study, we investigated the sorption properties of butanol onto three kinds of adsorbents with different polarities developed in our laboratory, that is, XD-41, H-511, and KA-I resin. The sorption behaviors of single component and ABE ternary mixtures presented in the fermentation broths on KA-I resin were investigated. KA-I resin had higher affinity for butanol than for acetone, ethanol, glucose, acetic acid, and butyric acid. Multicomponent ABE sorption on KA-I resin was modeled using a single site extended Langmuir isotherm model. In a desorption study, all the adsorbed components were desorbed in one bed volume of methanol, and the recovery of butanol from KA-I resin was 99.7%. The concentration of butanol in the eluent was increased by a factor of 6.13. In addition, KA-I resin was successfully regenerated by two bed volumes of water. Because of its quick sorption, high sorption capacity, low cost, and ease of desorption and regeneration, KA-I resin exhibits good potential for compatibility with future ABE fermentation coupled with in situ recovery product removal techniques. 相似文献
3.
Dai Jian-Ying Ma Lin-Hui Wang Zhuang-Fei Guan Wen-Tian Xiu Zhi-Long 《Bioprocess and biosystems engineering》2017,40(3):423-429
Bioprocess and Biosystems Engineering - Acetoin is a natural flavor and an important bio-based chemical which could be separated from fermentation broth by solvent extraction, salting-out... 相似文献
4.
Ataei SA Vasheghani-Farahani E 《Journal of industrial microbiology & biotechnology》2008,35(11):1229-1233
Lactic acid fermentation is an end product inhibited reaction. In situ separation of lactic acid from fermentation broth using
ion exchange resins was investigated and compared with conventional fermentation system. Amberlite resin (IRA-400, Cl−) was used to separate lactic acid from fermentation broth and pH was controlled online with an automatic pH controller. The
effect of process variables on lactic acid production by Lactobacillus casei in whey permeate was studied. The maximum productivity was obtained at pH = 6.1, T = 37 °C and impeller speed = 200 rpm. The maximum concentration of lactic acid at optimum condition was found to be 37.4 g/L
after 38 h of fermentation using in situ separation system. The productivity of in situ separation system was five times increased
in comparison with conventional system. 相似文献
5.
6.
Summary The suitability of extractive fermentation as a technique for the production of ethanol from lactose by Candida pseudotropicalis was examined as a potential improvement over conventional methods. A biocompatible solvent was selected through determination of the critical log P (octanol-water distribution coefficient) of the fermentation organism. Using Adol 85 NF, the selected solvent, extractive fed-batch and conventional fed-batch systems were operated for 160 h. The extractive system showed a 60% improvement in lactose consumption and ethanol production, as well as a 75% higher volumetric productivity. 相似文献
7.
Summary Cell separation by flocculation+sedimentation ofStreptoccocus
equisimilis cultivation for hyaluronate lyase recovery, was investigated as a function of the pH of the fermentation broth, using three different cationic flocculants. The polyelectrolyte Superfloc N-100 appears to be the best of the three flocculants tested; after treatmen of pH 6.0 and 120 min free sedimentation, the cells are sedimented at 20% of the initial volume and 80% of the volume remained as a clear supernatant without loss of enzyme activity. 相似文献
8.
Purification of nattokinase by reverse micelles extraction from fermentation broth: effect of temperature and phase volume ratio 总被引:1,自引:0,他引:1
Nattokinase is a novel fibrinolytic enzyme that is considered to be a promising agent for thrombosis therapy. In this study, reverse micelles extraction was applied to purify and concentrate nattokinase from fermentation broth. The effects of temperature and phase volume ratio used for the forward and backward extraction on the extraction process were examined. The optimal temperature for forward and backward extraction were 25°C and 35°C respectively. Nattokinase became more thermosensitive during reverse micelles extraction. And it could be enriched in the stripping phase eight times during backward extraction. It was found that nattokinase could be purified by AOT reverse micelles with up to 80% activity recovery and with a purification factor of 3.9. 相似文献
9.
10.
11.
Reverse osmosis is shown to be a viable process for the concentration of highly labile antibiotic solutions. Mathematical n10deJs of antibiotic inactivation by chemical and biological contamination are proposed and correlated with experimental data. Procedures for obtaining high antibiotic recoveries are discussed. 相似文献
12.
《Journal of Fermentation and Bioengineering》1989,67(5):321-323
A flocculent killer yeast, Saccharomyces cerevisiae strain H-1, which was selected for ethanol fermentation of beet molasses, has a tendency to lose its viability in distillery waste water (DWW) of beet molasses mash after ethanol fermentation. Through acclimations of strain H-1 in DWW, strain W-9, resistant to DWW, was isolated. Strain M-9, resistant to 2-deoxyglucose was further isolated through acclimations of strain W-9 in medium containing 150 ppm 2-deoxyglucose. A fermentation test of beet molasses indicated that the ethanol productivity and sugar consumption were improved by strain M-9 compared to the parental strain H-1 and strain W-9. The concentration of ethanol produced by strain M-9 was 107.2 g/l, and the concentration of residual sugars, which were mainly composed of sucrose and fructose, were lower than those produced by the parental strain H-1 and strain W-9 at the end of fermentation of beet molasses. 相似文献
13.
Diptarka Dasgupta Prasenjit Ghosh Debashish Ghosh Sunil Kumar Suman Rashmi Khan Deepti Agrawal Dilip K. Adhikari 《Bioprocess and biosystems engineering》2014,37(10):2019-2029
High temperature ethanol fermentation from sugarcane molasses B using thermophilic Crabtree-positive yeast Kluyveromyces sp. IIPE453 was carried out in batch bioreactor system. Strain was found to have a maximum specific ethanol productivity of 0.688 g/g/h with 92 % theoretical ethanol yield. Aeration and initial sugar concentration were tuning parameters to regulate metabolic pathways of the strain for either cell mass or higher ethanol production during growth with an optimum sugar to cell ratio 33:1 requisite for fermentation. An assessment of ethanol recovery from fermentation broth via simulation study illustrated that distillation-based conventional recovery was significantly better in terms of energy efficiency and overall mass recovery in comparison to coupled solvent extraction–azeotropic distillation technique for the same. 相似文献
14.
《Journal of Fermentation and Bioengineering》1990,69(1):39-45
Repeated-batch fermentation by a flocculating fusant, Saccharomyces cerevisiae HA 2, was done in a molasses medium that contained 20% (w/v) total sugar, at 30°C in an automatically controlled fermentor, and the effects of ethanol concentration on the specific growth rate and the specific production rate of ethanol were studied. Both the specific growth rate and the specific production rate of ethanol fell with increase of ethanol concentration, and there was a linear correlation between each rate and the concentration of thanol. The maximum specific growth rate (μmax) and the maximum specific production rate of ethanol (qmax) were 0.12 h−1 and 0.1 g ethanol/109 cells·h, respectively. The specific growth rate and the specific production rate of ethanol fell to zero at ethanol concentration of 89 g/l and 95 g/l, respectively. The number of viable cells, calculated from the linear inhibition equation, was 1.3 × 109 cells/ml for production of 85 g/l ethanol at a dilution rate (D1) of 0.2 h−1. Based on this estimation, a laboratory-scale continuous fermentation, using two fermentors in series, was done. In the second fermentor, 85 g/l ethanol was produced at a dilution rate (D1) of 0.2 h−1 by the active feedig of the fermented mash from the first fermentor into the second fermentor by pumping (hereafter called active feeding). To maintain the number of viable cells above 109 cells/ml in the second fermentor, a active feeding ratio of more than 23% was required. Under these conditions, 81 g/l ethanol was produced in the second fermentor at a dilution rate (Dt) of 0.25 h−1, and the high ethanol productivity of 20.3 g/l·h could be achieved. A bench-scale continuous fermentation, using two fermentors in series, with a active feeding ratio of 25% was done. An ethanol concentration of 84 g/l in the second fermentor at a dilution rate (Dt) of 0.25 h−1 was achieved, just as it was in the laboratory-scale fermentation test. 相似文献
15.
16.
17.
W. Borzani 《World journal of microbiology & biotechnology》1996,12(4):415-416
The efficiency of ethanol yield increased from 61% to 88% of the theoretical value as the filling-up time was approached in fed-batch fermentation with Saccharomyces cerevisiae. Temporary accumulation of ethanol within the yeast cells may explain the above variation.The author is with the Instituto Mauá de Tecnologia, Estrada das Lágrimas 2035, 09580-900. Sao Caetano Do Sul, SP, Brazil 相似文献
18.
粘杆菌素发酵液微滤膜分离处理过程研究 总被引:2,自引:0,他引:2
粘杆菌素由于其药性强、残留低、对人畜无害被认为是最安全的畜禽抗生素之一。利用微滤对粘杆菌素发酵液进行预处理,根据发酵液的特性,选择孔径为0.2μm、膜面积为0.06M。的管式陶瓷膜为微滤膜,研究了操作参数的适宜值:压力为0.2MPa,流量为20L/min,在浓缩倍数达到3.5倍时连续两次加入与浓缩液体等量的水,得率为96%。经微滤处理后,滤液的吸光度Abs(470nm)为0.394,N-NH,含量为115mg/100mL,有效地去除了菌体、胶体蛋白及部分色素等,效果优于工业生产中板框过滤滤液的质量标准。 相似文献
19.
20.
The in-situ recovery of ethanol from fermentation by different hydrophobic adsorbents has been studied. Liquid phase experiments have demonstrated that pentasil zeolites have a high selectivity for ethanol in the low concentration range. It is shown that the contact of the fermentation broth with the zeolite NaZSM-5 avoids the product inhibition. On the other hand, the ethanol production rate dramatically changes in dependence on the glucose-to-adsorbent ratio. 相似文献