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1.
Antioxidant activities and phenolics content of eight species of seaweeds from north Borneo 总被引:1,自引:0,他引:1
Patricia Matanjun Suhaila Mohamed Noordin Mohamed Mustapha Kharidah Muhammad Cheng Hwee Ming 《Journal of applied phycology》2008,20(4):367-373
The antioxidant activity of eight edible species of Malaysian North Borneo seaweeds obtained from Sabah waters (Kudat, Tanjung
Aru and Semporna) consisting of three red seaweeds (Eucheuma cottonii, E. spinosum and Halymenia durvillaei), two green seaweeds (Caulerpa lentillifera and C. racemosa) and three brown seaweeds (Dictyota dichotoma, Sargassum polycystum and Padina sp.) were determined. Methanol and diethyl ether were used as extraction solvent. The antioxidant activities were determined
by two methods, TEAC (trolox equivalent antioxidant capacity) and FRAP (ferric reducing antioxidant power) assays. The total
phenolic content of the extract was determined according to the Folin–Ciocalteu method and results were expressed as phloroglucinol
equivalents. The methanolic extracts of green seaweeds, C. lentillifera and C. racemosa, and the brown seaweed, S. polycystum showed better radical-scavenging and reducing power ability, and higher phenolic content than the other seaweeds. The TEAC
and FRAP assays showed positive and significantly high correlation (R
2 = 0.89). There was a strong correlation (R
2 = 0.96) between the reducing power and the total phenolic content of the seaweeds methanolic dry extracts. These seaweeds
could be potential rich sources of natural antioxidants. 相似文献
2.
M. E. Estrada-Zúñiga F. Cruz-Sosa M. Rodríguez-Monroy J. R. Verde-Calvo E. J. Vernon-Carter 《Plant Cell, Tissue and Organ Culture》2009,97(1):39-47
Plant tissue cultures represent a potential source for producing secondary metabolites. In this work, Buddleja cordata tissue cultures were established in order to produce phenylpropanoids (verbascoside, linarin and hydroxycinnamic acids),
as these metabolites are credited with therapeutic properties. Highest callus induction (76.4–84.3%) was obtained in five
treatments containing 2,4-Dichlorophenoxyacetic acid (2,4-d: 0.45–9.05 μM) with Kinetin (KIN: 2.32, 4.65 μM), whereas highest root induction (79.6%) corresponded to the α-Naphthaleneacetic
acid (9.05 μM) with KIN (2.32 μM) treatment. Verbascoside was the major phenylpropanoid produced in in vitro cultures (root,
white and green callus) [66.24–86.26 mg g−1 dry weight (DW)], while linarin and hydroxycinnamic acid production was low (0.95–3.01 mg g−1 DW). Verbascoside and linarin production were improved in cell suspension culture (116 mg g−1 DW and 8.12 mg g−1 DW, respectively). 相似文献
3.
Algal communities inhabiting four calamine mine spoils differing in time since cessation of exploitation and loaded with high
concentrations of zinc (20,284–61,599 μg g−1 soil DW), lead (2,620–3,885 μg g−1 DW) and cadmium (104–232 μg g−1 DW) were studied. In dump soils of slightly alkaline pH (7.28–7.52) and low nutrient (, , ) concentrations, chlorophyll a content ranged from 0.41 to 2.27 μg g−1 soil DW. In total, 23 algal species were recorded. Chlorophyta were the dominant taxonomic group (42–55% of all identified
species) followed by Cyanobacteria (28–36%) and Heterokontophyta (13–21%). The highest species richness (18) was observed
in the oldest dump (120 years old) with natural succession, while in younger dumps it was lower (11–15). Total algal abundance
ranged between 5.5 and 19.1 × 102 ind. g−1 soil DW, and values of Margalef’s diversity indices (1.59–2.25) were low. These results may suggest that both high concentrations
of heavy metals and low nutrient content influenced the algal communities in all the dumps studied. The differences in algal
microflora observed between tailing dumps may indicate that habitat quality improved with time and that algae isolated from
Zn/Pb-loaded soils may be Zn/Pb-resistant ecotypes of ubiquitous species. 相似文献
4.
The freshwater microalga Chlorella vulgaris was grown heterotrophically in fed-batch 50–600-L fermenters at 36°C, on aerated and mixed nutrient solution with urea as
a nitrogen and glucose as a carbon and energy source. Cell density increased from the initial value 6.25 to 117.18 g DW L−1 in 32 h in the fermenter 50 L at a mean growth rate 3.52 g DW L−1 h−1. The DW increase in the fermenter 200 L was from 7.25 to 94.82 g DW L−1 in 26.5 h at a mean growth rate 3.37 g DW L−1 h−1. Mean specific growth rate μ was about 0.1 h−1 in the both fermenters, if nutrients and oxygen were adequately supplied. The DW increase in the fermenter 600 L was from
0.8 to 81.6 g DW L−1 in 66.5 h at a mean growth rate 1.22 g DW L−1 h−1 and μ = 0.07 h−1. A limitation of the cell growth rate in 600 L fermenter caused by a low dissolved oxygen concentration above cell densities
higher than 10 g DW L−1) occurred. Specific growth rate decreased approximately linearly with increasing glucose concentration (25–80 g glucose L−1) at the beginning of cultivation and decreased with the time of cultivation. The cell yield was 0.55–0.69 g DW (g glucose)−1. The content of proteins, β-carotene, and chlorophylls in the cells steadily increased and starch content decreased, by keeping
aerated and mixed culture another 12 h in fermenter after the cell growth was stopped due to glucose deficiency. 相似文献
5.
Marzena Wielanek Aleksandra Królicka Katarzyna Bergier Ewa Gajewska Maria Sk?odowska 《Biotechnology letters》2009,31(6):917-921
Hairy roots of Nasturtium officinale, Barbarea verna and Arabis caucasica with active glucosinolate-myrosinase system were obtained after transformation with Agrobacterium rhizogenes. Hairy roots of N. officinale produced phenylalanine-derived gluconasturtiin and glucotropaeolin (max. 24 and 7 mg g−1 DW). B. verna and A. caucasica hairy roots produced gluconasturtiin (max. 41 mg g−1 DW) and methionine-derived glucoiberverin (max. 32 mg g−1 DW), respectively. Treatment of the roots with amino acid precursors of glucosinolate or/and cysteine biosynthesis increased
levels of glucosinolate production, combinations of phenylalanine with cysteine (for gluconasturtiin and glucotropaeolin)
and methionine with o-acetylserine (for glucoiberverin) were the most effective. 相似文献
6.
Adventitious roots of Echinacea purpurea were cultured in airlift bioreactors (20 l, 500 l balloon-type, bubble bioreactors and 1,000 l drum-type bubble bioreactor)
using Murashige and Skoog (MS) medium with 2 mg indole butyric acid l−1 and 50 g sucrose l−1 for the production of chichoric acid, chlorogenic acid and caftaric acid. In the 20 l bioreactor (containing 14 l MS medium)
a maximum yield of 11 g dry biomass l−1 was achieved after 60 days. However, the amount of total phenolics (57 mg g−1 DW), flavonoids (34 mg g−1 DW) and caffeic acid derivatives (38 mg g−1 DW) were highest after 50 days. Based on these studies, pilot-scale cultures were established and 3.6 kg and 5.1 kg dry biomass
were achieved in the 500 l and 1,000 l bioreactors, respectively. The accumulation of 5 mg chlorogenic acid g−1 DW, 22 mg chichoric acid g−1 DW and 4 mg caftaric acids g−1 DW were achieved with adventitious roots grown in 1,000 l bioreactors. 相似文献
7.
C. S. Jeong H. N. Murthy E. J. Hahn H. L. Lee K. Y. Paek 《Acta Physiologiae Plantarum》2009,31(1):219-222
The effect of the root-inoculum size and axuin concentration on growth of adventitious roots and accumulation of ginsenosides
were studied during suspension cultures of ginseng (Panax ginseng C.A. Meyer). Of the various concentrations of indole-3-butyric acid (IBA) and γ-naphthaleneacetic acid (NAA) used as supplementary
growth regulators along with Murashige and Skoog medium, 25 μM IBA was found suitable for lateral root induction and growth,
as well as accumulation of ginsenosides. Inoculum size of 5 g L−1 was found suitable for optimal biomass (10.5 g L−1 dry biomass) and ginsenosides (5.4 mg g−1 DW) accumulation. Of the various length of root inocula tested (chopped to 1–3, 4–6, 7–10 mm and un-chopped), root inocula
of 7–10 mm was found suitable for biomass and ginsenoside accumulation. 相似文献
8.
In vitro cultures of Berberis buxifolia were established using thidiazuron (4.5, 23 and 45 mM) or picloram (4 and 40 mM) as plant growth regulators for sustaining
growth. For producing berberine, a two-stage culture was performed. In the first step, thidiazuron or picloram were used for
biomass production followed by the production stage where benzylaminopurine (4.4 mM) was added as a plant growth regulator.
Berberine yields (102 mg g−1 DW) and in vitro shoot cultures (200 mg g−1 DW) were significantly lower than those of whole plants in the field (416 mg g−1 DW). The highest productivity (0.18 mg 1−1 day−1) was attained using picloram (either 4 on 40 mM) in the first stage for producing biomass. 相似文献
9.
Le Dinh Hung Kanji Hori Huynh Quang Nang Tran Kha Le Thi Hoa 《Journal of applied phycology》2009,21(3):265-272
The three color morphotypes of the red alga Kappaphycus alvarezii (brown, red and green) were cultured in Camranh Bay, Vietnam, using the fixed off-bottom monoline culture method to evaluate
the growth rate, carrageenan yield, 3,6-anhydrogalactose, gel strength and lectin content. The brown morphotype was cultivated
over a 12-month period; the red and green morphotypes were over a 6-month period. At the 60-day culture timepoint, the brown
morphotype showed a higher growth rate (3.5–4.6% day−1) from September to February, and lower growth rate (1.6–2.8% day−1) from March to August. Significant (P < 0.05) differences in growth rate between culture months were found with the brown morphotype. High growth rates for the
red (3.6–4.4% day−1) and green (3.7–4.2% day−1) morphotypes were obtained from September to February. The carrageenan yield, 3,6-anhydrogalactose and gel strength of the
three morphotypes showed little variation, with the highest values obtained in November–December. At the 30-day sampling point,
the brown morphotype had a higher lectin content (167–302 μg g−1 dry alga) from August to March and a lower lectin content (23–104 μg g−1 dry alga) from April to July. High lectin contents were recorded for the red (139–338 μg g−1 dry alga) and green (124–259 μg g−1 dry alga) morphotypes from September to February. This study shows that the different morphotypes of K. alvarezii can be grown in the tropical waters of the Camranh during the northeast monsoon, and part of the southwest monsoon, especially
the brown morphotype, which can be grown during any season. 相似文献
10.
Yantree Devi Sankar-Thomas Reinhard Lieberei 《Plant Cell, Tissue and Organ Culture》2011,106(3):445-454
Levels of camptothecin (CPT) and 10-hydroxycamptothecin (HCPT) were determined in different cultures of Camptotheca acuminata grown either in a Temporary Immersion System (TIS) or on solid medium. CPT was also detected in liquid culture medium. HPLC
analysis showed significant differences in CPT contents in all tissues analysed and the highest CPT contents were found in
shoots grown on solid medium and in TIS with a mean of 2.2 and 2.5 mg g−1 DW, respectively. The highest content of CPT detected in seedlings was 1.96 mg g−1 DW; while that of somatic embryos at cotyledonary stage and regenerated plants were 0.87 and 1.23 mg g−1 DW, respectively. It was also shown that shoots cultured in TIS secreted substantial amount of CPT into the liquid medium.
After 4 weeks in culture a mean of 6, 05 and 12, 6 μg g−1 FW were determined at 4 and 8 immersion cycles daily (IC d−1), respectively. This aspect opens new possibilities regarding the isolation of CTP using TIS culture systems. 相似文献
11.
T. Hura F. Dubert T. Dąbkowska E. Stupnicka-Rodzynkiewicz A. Stokłosa A. Lepiarczyk 《Acta Physiologiae Plantarum》2006,28(6):537-545
The purpose of this study was to determine the content of selected phenolic compounds in white mustard, buckwheat, spring
barley, oat and rye grown under field conditions. Moreover, the allelopathic efficiency of these compounds was evaluated by
sensitivity of Echinochloa crus-galli. The aromatic acids: trans-cinnamic, salicylic, ferulic, chlorogenic, p-hydroxybenzoic, protocatechuic, p-coumaric and vanillic were separated from
crop plants by TLC and determined spectrophotometrically.
Differences in concentrations of analysed compounds were observed for most of the examined plant species. The highest concentration
was noticed for cinnamic acid and ranged from 360 μg·g−1 DW in rye to 2770 μg·g−1 DW in spring barley. The relatively high concentration was noticed for ferulic acid (from 73.8 μg·g−1 DW in buckwheat to 1046 μg·g−1 DW in spring barley) and p-coumaric acid (from 50 μg·g−1 DW in oat to 1499 μg·g−1 DW in buckwheat). The observed differences in the phenolics content between two successive vegetation seasons can reflect
the effect of abiotic and biotic environmental factors on the phenolics level in studied plants.
In the greenhouse experiment the effect of particular compounds on the growth of Echinochloa crus-galli was also studied. It has been found that the examined phenolics, and especially trans-cinnamic acid and mixture of phenolic compounds, significantly inhibit the growth of Echinochloa crus-galli. The obtained results may contribute to the explanation of the biological activity of some phenolic compounds. 相似文献
12.
Dietary fibres from Ulva lactuca (L.) Thuret (sea lettuce) and Enteromorpha compressa (L.) Grev. (A.O. nori) were measured according to a ‘standard’ method and a ‘physiological’ protocol simulating the gastric
and intestinal environments. U. lactuca contained 15.8–8.0% soluble and 24.2–32.6% insoluble fibres according to the ‘standard’ and ‘physiological’ methods, respectively.
For E. compressa, these values were 14.9–15.9 and 21.6–28.7%, respectively. For both algae, the composition suggests that the soluble fibres
were xylorhamnoglycuronans sulphates and insoluble fibres were essentially composed of glucans. No marked chemical compositional
variation was observed between soluble fractions extracted under the simulated gastric and intestinal conditions. Fibres in
both algae are hydrophilic but the water holding capacities were higher after extraction of soluble fibres (5.5–9.5 g g−1 for the dry algae; 14.0–16.0 g g−1 for the standard insoluble fibres). Water soluble fibres demonstrated low intrinsic viscosities at 37 °C in buffers, particularly
those from E. compressa (36.0–36.5 ml g−1), and was affected by pH for those of U. lactuca (147.5 ml g−1 at pH 3.0 and 175.0 ml g−1 at pH 7.3). 相似文献
13.
Song Quan Wu Mei Lan Lian Ri Gao So Young Park Xuan Chun Piao 《In vitro cellular & developmental biology. Plant》2011,47(6):719-724
Astragalus membranaceus is one of the most widely used traditional medicinal herbs in China, but the time required to generate a useful product in
the field production is long. The growth of adventitious root cultures was compared between cultures grown in solid, liquid,
or a 5-L balloon-type bubble bioreactor. The maximum growth ratio (final dry weight/initial dry weight) was determined for
adventitious roots grown in the bioreactor. Studies carried out to optimize biomass production of adventitious roots compared
adventitious root growth from various inoculum root lengths, inoculum densities, and aeration volume in the bioreactors. The
maximum growth ratio occurred in treatments with a 1.5-cm inoculum root length, with 30 g (fresh weight) of inoculum per bioreactor
or with an aeration volume of 0.1 vvm (air volume/culture medium volume per min). The polysaccharide, saponin, and flavonoid
content of roots from bioreactor-grown cultures were compared to roots from field-grown plants grown for 1 and 3 yr. Total
polysaccharide content of adventitious roots in the bioreactor (30.0 mg g−1 dry weight (DW)) was higher than the roots of 1-yr-old (13.8 mg g−1 DW) and 3-yr-old (21.1 mg g−1 DW) plants in the field. Total saponin (3.4 mg g−1 DW) and flavonoid (6.4 mg g−1 DW) contents were nearly identical to 3-yr-old roots and higher than that of 1-yr-old roots under field cultivation. 相似文献
14.
Microbially produced extracellular polymeric substances (EPS) have been linked with many important ecological functions in
natural sediments; yet, most information has been derived from marine systems. The present paper is the first comprehensive
study on EPS (i.e., carbohydrates and proteins) dynamics in riverine sediments addressing spatial (six reservoirs and four
groyne fields across three European rivers), temporal (all seasons in 2003–2005), and vertical (over a 50-cm sediment depth
transect) pattern. The variation in hydrodynamic regime found in the reservoirs and groyne fields was reflected in the biomass
and composition of the benthic microorganisms that produce EPS. The microphytobenthic communities consisted mainly of diatoms
and a higher algal biomass (up to 248 μg g−1 dry weight, DW) seemed to be indicative for higher amounts of secreted colloidal carbohydrates. Consequently, the model proposed
by Underwood and Smith (1998) for the relation chlorophyll–colloidal carbohydrates was also applicable for upper riverine sediment layers. The close relation
between algal biomass and bacterial cell counts (108–109 cells g−1 DW) supports the idea of bacterial use of the secreted EPS. However, the data also suggest a contribution to the EPS pool
through bacterial secretion of proteins/extracellular enzymes and possibly carbohydrates. Over depth, the relationships between
microorganisms and EPS became increasingly decoupled along with increasing ratios of bound (refractory) to colloidal (labile)
EPS. These data suggest fresh production of polymeric substances in upper sediment layers and mainly accumulation of refractory,
biodegraded material in deeper layers. The high contents of EPS colloidal and bound carbohydrates (0.1–1.8 and 1.3–6.7 mg
g−1 DW, respectively) and EPS proteins (0.4–12.9 mg g−1 DW) at the freshwater study sites might indicate an important role in sediment ecology. 相似文献
15.
Md. Abdullahil Baque Abdullah Elgirban Eun-Jung Lee Kee-Yoeup Paek 《Acta Physiologiae Plantarum》2012,34(2):405-415
The effect of initial sucrose concentration was investigated in root suspension cultures of Morinda citrifolia to improve root growth and secondary metabolites production, i.e. anthraquinone, phenolics and flavonoids. Besides, oxidative
stress level, antioxidant enzymes activity and membranes damage under different sucrose concentration were estimated. A 5%
sucrose supply was shown to be optimal for the production of root dry mass, but higher sucrose concentrations of 7–9% inhibited
the accumulation of root dry weight (DW). However, the maximum production of anthraquinone (251.89 g L−1 DW), phenolics (165.14 g L−1 DW) and flavonoids (163.56 g L−1 DW) were achieved at 1% sucrose-treated culture, which may be a source carbon skeletons for secondary metabolism. At the
same time was observed low oxidative damage, which could be associated with high levels of secondary metabolites and the increased
activity of catalase. Although, catalase (CAT) activity were stimulated at 7–9% sucrose-treated cultures, high accumulation
of hydrogen peroxide (H2O2) and peroxidation of lipid (MDA) was induced. The observed high activity of CAT and guaiacol peroxidase (G-POD) were not
sufficient enough to mitigate the toxic effect of H2O2. 相似文献
16.
We quantified cover, population densities, size distribution and biomass of zebra mussels along 7 transects in eutrophic Lake
Ekoln (Sweden). We also analyzed the elemental (C, N, P) composition of zebra mussel soft tissue and computed their retention
rates of N and P their quantitative role in the lake’s nutrient budget. We hypothesized that zebra mussels play an important
role in the nutrient budget of the lake and speculate that the successive harvesting of cultured mussels could contribute
to the lake’s rate of recovery from cultural eutrophication. At depths exceeding 5 m, mussels covered consistently less than
5% or were absent. Similarly, mean densities were 3,158 ± 2,143 ind m−2 between 2 and 4 m, but rapidly declined at larger depths. Calculated clearance rates averaged 19.4 ± 2.3 km3 y−1, implying the entire lake is filtered every 8–10 days. Concentrations of N and P in mussel soft tissue averaged 100.9 ± 1.5 mg N g−1 DW and 9.3 ± 0.2 mg P g−1 DW. The lake population was estimated to 22.2 ± 2.6 × 1010 mussels, corresponding to a standing stock biomass of 362 ± 42 ton DW, or conservative estimates of 36.6 ± 4.3 ton N and
3.4 ± 0.4 ton P. Assuming a life span of 2–3 years gives a retention estimate of 1.2–1.8 ton P y−1 by mussels, corresponding to 50–77% of the annual P influx from Uppsala sewage treatment plant to the lake. Similarly, annual
N-retention by zebra mussels makes up 13–20 ton N y−1, largely equaling the annual N-deposition from atmospheric sources on the lake’s surface. These retention rates correspond
to only a few percent of the annual P-load from agricultural sources, but we argue that the quantitative role of zebra mussels
in nutrient budgets is much larger if these budgets are adjusted for the bias introduced by coarse estimates of N and P pools
that include a large share of refractory P. 相似文献
17.
Arribére MA Ribeiro Guevara S Bubach DF Arcagni M Vigliano PH 《Biological trace element research》2008,122(1):42-63
A survey of mercury (Hg) and selenium (Se) contents was performed in fish collected from lakes located in two National Parks
of the northern patagonian Andean range. Two native species, catfish (Diplomystes viedmensis) and creole perch (Percichthys trucha), and three introduced species, brown trout (Salmo trutta), rainbow trout (Oncorhynchus mykiss), and brook trout (Salvelinus fontinalis), were caught from lakes Nahuel Huapi, Moreno, Traful, Espejo Chico, and Guillelmo belonging to Nahuel Huapi National Park
and from lakes Futalaufquen and Rivadavia, Los Alerces National Park. In lake Moreno, fish diet items were analyzed and rainbow
trout grown in a farm. Hg and Se were measured in muscle and liver tissues by instrumental neutron activation analysis. The
average concentrations in muscle of Hg for all species, ages, and lakes are between 0.4 to 1.0 μg g−1 dry weight (DW) with a few fish, mainly native, exceeding the United States Environmental Protection Agency health advisory
for freshwater fish limited consumption, and from 0.8 to 1.5 μg g−1 DW for Se. Average concentrations in liver of Hg in all species range from 0.4 to 0.9 μg g−1 DW. Brown trout, the top predator in these lakes, showed the lowest average Hg burden in both tissues. Se concentrations
in the liver of brown and rainbow trout, up to 279 μg g−1 DW, are higher than those expected for nearly pristine lakes, exceeding 20 μg g−1 DW, the threshold concentration associated with Se toxicity. These species show lower Hg contents in muscle, suggesting a
possible detoxification of Hg by a Se-rich diet. Creole perch and velvet catfish livers have lower Se concentrations, with
a narrower span of values (2.3 to 8.5 μg g−1 and 3.3 to 5.5 μg g−1 DW respectively). 相似文献
18.
Justyna Krzyzanowska Anna Czubacka Lukasz Pecio Marcin Przybys Teresa Doroszewska Anna Stochmal Wieslaw Oleszek 《Plant Cell, Tissue and Organ Culture》2012,108(1):73-81
The effects of two elicitors: jasmonic acid and methyl jasmonate on cell growth as well as on rosmarinic acid accumulation
in cell suspension cultures of Mentha × piperita were investigated. The highest rosmarinic acid accumulation 117.95 mg g−1 DW (12% DW) was measured 24 h after addition of 100 μM methyl jasmonate. A similar concentration 110.12 mg g−1 DW was detected 48 h after application of 200 μM jasmonic acid. Those values were nearly 1.5 times higher compared to the
control sample, without elicitation. There was no substantial influence of elicitors on rosmarinic acid secretion into the
culture media. Extracellular concentrations of rosmarinic acid were similar to the values from the control variants. It was
documented that suspension cultures of M. piperita treated with elicitors showed a decrease in biomass accumulation when compared to the control. 相似文献
19.
Chavan SP Lokhande VH Nitnaware KM Nikam TD 《Applied microbiology and biotechnology》2011,89(6):1701-1707
The present study examined the effects of plant growth hormones, incubation period, biotic (Trametes versicolor, Mucor sp., Penicillium notatum, Rhizopus stolonifer, and Fusarium oxysporum) and abiotic (NaCl, MgSO4, FeSO4, ZnSO4, and FeCl3) elicitors on cell growth and α-tocopherol and pigment (red and yellow) productions in Carthamus tinctorius cell cultures. The cell growth and α-tocopherol and pigment contents improved significantly on Murashige and Skoog (MS) liquid
medium containing 50.0 μM α-naphthalene acetic acid (NAA) and 2.5 μM 6-Benzyladenine (BA) at 28 days of incubation period.
Incorporation of T. versicolor (50 mg l−1) significantly enhanced the production of α-tocopherol (12.7-fold) and red pigment (4.24-fold). Similarly, supplementation
of 30 mg l−1
T. versicolor (7.54-fold) and 70 mg l−1
Mucor sp. (7.40-fold) significantly increased the production of yellow pigment. Among abiotic elicitors, NaCl (50–70 mg l−1) and MgSO4 (10–30 mg l−1) significantly improved production of α-tocopherol (1.24-fold) and red pigment (20-fold), whereas yellow pigment content
increased considerably by all the abiotic elicitor treatments. Taken together, the present study reports improved productions
of α-tocopherol and the pigment as a stress response of safflower cell cultures exposed to these elicitors. 相似文献
20.
Natasha Maurmann Carina M. B. De Carvalho Andréia Loviane Silva Arthur G. Fett-Neto Gilsane L. von Poser Sandra B. Rech 《In vitro cellular & developmental biology. Plant》2006,42(1):50-53
Summary
Valeriana glechomifolia is an endemic species of southern Brazil, capable of accumulating, in all of its organs, the terpene derivatives known as
valepotriates, the presumed sedative components of the roots of pharmaceutically used species of Valeriana. In vitro cultures of the plant were established and the accumulation of acevaltrate, didrovaltrate, and valtrate in callus, cell suspension,
and untransformed root cultures was studied. Leaves of in natura plants and roots of micropropagated plantlets were used as the explants for callus induction and root culture establishment,
respectively, on Gamborg B5 basal medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) alone or with kinetin (KIN).
Culture growth and secondary metabolite yields were enhanced with 2,4-D (4.52μM) and KIN (0.93μM). Maximum valepotriate contents, quantified by HPLC, of acevaltrate (ACE) 2.6mg g−1 DW, valtrate (VAL) 10.2mgg−1 DW, and didrovaltrate (DID) 2.9mg g−1 DW were observed in root cultures after 7–8wk of culture. 相似文献