Flora Palinologica Italiana,Sezione Aeropalinologica —S 238:Juglans regia L. (Juglandaceae)

Riassunto In accordo con il programma ?Flora Palinologica Italiana, Sezione Aeropalinologica? viene presentata la scheda morfopalinologica diJunglans regia L. (noce) relativa a polline fresco ed acetolizzato.      

Periods of organogenesis in mono- and bicyclic annual shoots of Juglans regia L. (Juglandaceae)

The organogenetic cycle of shoots on main branches of 4-year-old Juglans regia trees was studied. Mono- and bicyclic floriferous and vegetative annual shoots were analysed. Five parent annual shoot types were sampled between October 1992 and August 1993. Organogenesis of summer growth units was monitored between 16 Jun. and 3 Aug. 1993. Variations over time in the number of nodes, cataphylls and embryonic green leaves of terminal buds were studied. The number of nodes of parent shoot buds was compared with the number of nodes of shoots derived from parent shoot buds. The spring growth units of mono- and bicyclic shoots consist exclusively of preformed leaves which were differentiated, respectively, during the spring flush of growth (mid-April until mid-May) or the summer flush of growth (mid-June until early August) in the previous growing season. Thus, winter buds may consist of flower and leaf primordia differentiated in two different periods during annual shoot extension. The summer growth units of bicyclic shoots consist of preformed leaves that were differentiated in spring buds during the spring flush of growth in the current growing season. Bud morphology is compared between spring and summer shoots.     

Strategies for agrobiodiversity conservation and promotion: a case from Yunnan, China

This paper deals with strategies for agrobiodiversity conservation and promotion based on studies on four plant groups (selected from 50 plant groups) occurring in the Yunnan Province of China. These plants are edible konjac (Amorphophallus), medicinal Paris, Musella lasiocarpa and wild tea (Camellia sinensis and its wild relatives), including their cultivars and wild populations. After analyzing the target plants, we conclude that the following strategies should be adopted to conserve and promote agrobiodiversity: (1) in situ conservation of agrobiodiversity, including habitat protection of wild populations, maintenance of native species and varieties in traditional agroecosystems, and relevant environmental education; (2) ex situ conservation and promotion of agrobiodiversity, including establishment of living collections and germplasm banks, and introduction of species and varieties into agroecosystems for agricultural practice; and (3) promotion and conservation of agrobiodiversity through sustainable uses, including technique development of propagation, cultivation, pest and disease control, on farm and off farm management, and other activities such as new variety breeding and scientific studies. Strategies developed here will be helpful to conserve and promote agrobiodiversity at agroecosystem, species, variety or landrace, and management system levels.     

Population genetic structure and adaptive differentiation of iron walnut Juglans regia subsp. sigillata in southwestern China

Southwestern (SW) China is an area of active tectonism and erosion, yielding a dynamic, deeply eroded landscape that influences the genetic structure of the resident populations of plants and animals. Iron walnut (Juglans regia subsp. sigillata) is a deciduous tree species endemic to this region of China and cultivated there for its edible nuts. We sampled 36 iron walnut populations from locations throughout the species' range in SW China and genotyped a total of 765 individuals at five chloroplast DNA regions and 22 nuclear microsatellite loci. Species distribution models were produced to predict the evolution and historical biogeography of iron walnut and to estimate the impacts of climate oscillations and orographic environments on the species' demography. Our results indicated that J. regia subsp. sigillata had relatively low genetic diversity, high interpopulation genetic differentiation, and asymmetric interpopulation gene flow. Based on DIYABC analysis, we identified two lineages of J. sigillata in southwestern China. The lineages (subpopulations) diverge during the last glacial period (~1.34 Ma). Southwestern China was a glacial refuge during the last glacial period, but increasingly colder and arid climates might have fostered the fragmentation of J. regia subsp. sigillata within this refugium. Finally, we found that recent habitat fragmentation has led to a reduction in population connectivity and increased genetic differentiation by genetic drift in isolated populations. Our results support a conclusion that geological and climatic factors since the Miocene triggered the differentiation, evolutionary origin, and range shifts of J. sigillata in the studied region.     

The relationship between phenology of pistillate flower organogenesis and mode of heterodichogamy in Juglans regia L. (Juglandaceae)

 We investigated the degree of organogenesis completed at the end of the growing season in pistillate flowers of heterodichogamous Juglans regia, English or Persian walnut. Terminal buds from paired cultivars, one each protandrous and protogynous, chosen to represent early, midseason and late leafing walnuts, were examined by scanning electron microscopy. Results indicate that pistillate floral primordia in protandrous individuals had not progressed beyond involucre initiation during the season prior to bloom. In protogynous individuals, floral differentiation had progressed to the initiation of perianth primordia. These observations are compared with an earlier report on staminate flower differentiation in the same cultivars where a comparable, but opposite, relationship exists. We conclude that the degree of differentiation in both staminate and pistillate flowers that must be completed between the time growth resumes in the spring and anthesis is a developmental determinant of the mode of heterodichogamy in walnut. Received: 15 June 1996 / Revision accepted: 25 October 1996     

Bud Structure in Relation to Shoot Morphology and Position on the Vegetative Annual Shoots of Juglans regia L. (Juglandaceae)

The length and basal diameter of all lateral and terminal budsof vegetative annual shoots of 7-year-oldJuglans regia treeswere measured. All buds were dissected and numbers of cataphylls,embryonic leaves and leaf primordia were recorded. Each axillarybud was ranked according to the position of its associated leaffrom the apex to the base of its parent shoot. Bud size andcontent were analysed in relation to bud position and were comparedwith the size and number of leaves of shoots in equivalent positionswhich extended during the following growing season. Length andbasal diameter of axillary buds varied according to their positionon the parent shoot. Terminal buds contained more embryonicleaves than any axillary bud. The number of leaves was smallerfor apical and basal axillary buds than for buds in intermediatepositions on the parent shoot only. All new extended shootswere entirely preformed in the buds that gave rise to them.Lateral shoots were formed in the median part of the parentshoot. These lateral shoots derived from buds which were largerthan both apical and basal ones. Copyright 2001 Annals of BotanyCompany Juglans regia L., Persian walnut tree, branching pattern, preformation, bud content, shoot morphology     

Comparative flower development of Juglans regia,Cyclocarya paliurus and Engelhardia spicata: homology of floral envelopes in Juglandaceae

Homology among reproductive structures is pivotal for understanding angiosperm evolution. In Juglandaceae, homologies of floral parts have been disputed due to morphological variability in flowers. Previous interpretations were based mainly on mature flowers and lacked developmental studies. We investigated the unisexual flower development of Juglans regia, Cyclocarya paliurus and Engelhardia spicata using scanning electron microscopy. The ‘floral envelope’ of staminate flowers in J. regia and C. paliurus consists of a bract and several tepals. Six tepals are initiated in a whorled pattern in J. regia, whereas four to six tepals are arranged in a variable pattern in C. paliurus. The three‐lobed bract of E. spicata results from the cleavage of an entire bract, rather than adnation of the bract and its bracteoles. Pistillate flowers of J. regia and C. paliurus, subtended by a bract and a horseshoe‐shaped bracteole, usually have four tepals initiating simultaneously in one whorl. The organogenesis patterns of the ‘floral envelope’ and stamens in staminate flowers show greater diversity, indicating different degrees of reduction among genera. Our floral developmental data support the division of Juglandaceae into two subfamilies and then further into three tribes.     

Mating patterns and pollen dispersal in a heterodichogamous tree, Juglans mandshurica (Juglandaceae)

Mating patterns in heterodichogamous species are generally considered to be disassortative between flowering morphs, but this hypothesis has hitherto not been vigorously tested. Here, mating patterns and pollen dispersal were studied in Juglans mandshurica, a heterodichogamous wind-pollinated species that is widely distributed in northern and north-eastern China. Paternity analyses carried out on 11 microsatellite loci were used to estimate morph-specific rates of outcrossing and disassortative mating. Pollen dispersal and genetic structure were also investigated in the population under study. The mating pattern of J. mandshurica was highly outcrossing and disassortative. Pairwise values of intramorph relatedness were much higher than those of intermorph relatedness, and a low level of biparental inbreeding was detected. There was no significant difference in outcrossing and disassortative mating rates between the two morphs. The effective pollen dispersal distribution showed an excess of near-neighbor matings, and most offspring of individual trees were sired by one or two nearby trees. These results corroborate the previous suggestion that mating in heterodichogamous plant species is mainly disassortative between morphs, which not only prevents selfing but also effectively reduces intramorph inbreeding.     

Species composition,trend of biodiversity variation and conservation of the fish in Lijiang River (in China)

In the present study, diversity investigations were conducted for fish species in Lijiang River main stream and its four tributaries for four times seasonally during the period from April 2013 to January 2015. Based upon the 19763 fish samples collected, a total of 91 species and subspecies belonging to five orders, 17 families and 65 genera, had been recorded. According to the method of inland water fishery natural resource investigation, by means of mathematics statistics and species diversity index to analyze data, studied the fish biodiversity characteristics and trend of biodiversity variation. On the basis, we found the reasons of exhaustion of fish resources and provided the scientific basis to protect and repair the aquatic ecosystem of Lijiang River. The research demonstrated that the diversity index of fish species in Lijiang River was at a high level, but due to the superposition of various risk factors, including overexploitation, water pollution, habitat alteration, etc., increasing exhaustion of fish stocks and decreasing significant of fish species diversity. It was urgent to take measures to protect and restore the aquatic ecosystem of Lijiang River.     

The trichomes of pericarp in Juglans (Juglandaceae): scanning microscopy, fluorescent microscopy amd histochemistry

Four types of glandular and non-glandular trichomes of pericarp in four Juglans species (J. ailanthifolia, J. cordiformis, J. mandshurica and J. regia) from Juglandaceae were studied by scanning electron microscopy, fluorescent light microscopy and histochemistry. The capitate trichomes on short stalk, the capitate trichomes on long stalk and the peltate trichomes belong to glandular trichomes; the simple hairs concern to non-glandular trichomes. The investigated species differ one from another in dimensions and distribution oftrichomes as well as the chemical content and the mechanism of secretion. The fluorescent markers and histochemical tests show the presence of flavonoids, tannins and polyphenols in trichomes on short and long stalk. In peltate trichomes the flavonoids and tannins were found in lesser quantity and the polyphenols are absent. In simple hairs the phenolic substances have not been recognized. It has been come out with the suggestion about a functional role of each type of trichomes.     

A new genomic tool for walnut (Juglans regia L.): development and validation of the high‐density Axiom™ J. regia 700K SNP genotyping array

Over the last 20 years, global production of Persian walnut (Juglans regia L.) has grown enormously, likely reflecting increased consumption due to its numerous benefits to human health. However, advances in genome‐wide association (GWA) studies and genomic selection (GS) for agronomically important traits in walnut remain limited due to the lack of powerful genomic tools. Here, we present the development and validation of a high‐density 700K single nucleotide polymorphism (SNP) array in Persian walnut. Over 609K high‐quality SNPs have been thoroughly selected from a set of 9.6 m genome‐wide variants, previously identified from the high‐depth re‐sequencing of 27 founders of the Walnut Improvement Program (WIP) of University of California, Davis. To validate the effectiveness of the array, we genotyped a collection of 1284 walnut trees, including 1167 progeny of 48 WIP families and 26 walnut cultivars. More than half of the SNPs (55.7%) fell in the highest quality class of ‘Poly High Resolution’ (PHR) polymorphisms, which were used to assess the WIP pedigree integrity. We identified 151 new parent‐offspring relationships, all confirmed with the Mendelian inheritance test. In addition, we explored the genetic variability among cultivars of different origin, revealing how the varieties from Europe and California were differentiated from Asian accessions. Both the reconstruction of the WIP pedigree and population structure analysis confirmed the effectiveness of the Applied Biosystems? Axiom? J. regia 700K SNP array, which initiates a novel genomic and advanced phase in walnut genetics and breeding.     

Numerical taxonomy of Palaeocarya (Juglandaceae) from the Mangbang Formation of West Yunnan, China

Specimens of Palaeocarya (Juglandaceae) were collected from the upper Pliocene of western Yunnan Province, which is a new geographical and age range record of engelhardioid winged fruits in China. A numerical taxonomic investigation based on 14 morphological characters of fossil and extant specimens of engelhardioid winged fruits in China was conducted using multivariate methods. Phenetic clustering shows that there are four morphotypical groups which we assign to four species. Two groups are assigned to the previously described fossil species, Palaeocarya guangxiensis Li Hao-Min et Chen Yun-Fa, 2003 and Palaeocarya koreanica (Oishi) Manchester, 1987. Two new species are recognized, Palaeocarya yunnanensis and Palaeocarya longialata. These Neogene Engelhardieae fruits provide fossil data for understanding the origin and evolution of the Engelhardia species that now inhabit South China.     

云南横脊叶蝉亚科三新种记述(同翅目:叶蝉科)

记述了采自云南的横脊叶蝉亚科Evacanthinae,斜脊叶蝉属Bundera Distant 2新种,即红条斜脊叶蝉B.rufistriana sp.nov.和透斑斜脊叶蝉B.pellucida sp.nov。以及角突叶蝉属Taperus Li et Wang 1新种,即兰坪角突叶蝉T.lanpingensis sp.nov.模式标本保存在贵州大学昆虫研究所。     

我国伊蚊—新亚属及—新种(双翅目:蚊科)

1985年6月,在进行滇西类业区系调查中采获一批伊蚊,经鉴定,发现1新亚属,现将该亚属及1新种描述如下。华蚊亚属Sinoaedes,新亚属 亚属特征:雌蚊 中型,暗黑色,具银白鳞饰。触须暗色。喙暗色。前胸前背片平覆宽白鳞。中胸盾片平覆褐色窄鳞;小盾片三叶状,具宽鳞;后背片光裸。有气门后鬃;有1根中胸下后鬃。翅鳞暗色。各足除股节腹面具白色区外,一致暗色。腹节暗色。第     

云南异丽金龟属新种记述（鞘翅目：丽金龟科）

记述采自云南省的丽金龟科异丽金龟属的１７个新种。     

An Alien Species,Sisymbrium orientale (Brassicaceae) , Emerged in Yunnan,China

A alien plant species , Sysimbrium orientale L . (Brassicaceae ) is recorded in Yunnan , China in the paper . The species has been found invading the ecosystems of croplands and grasslands in Australia . Although it was previously recorded in China, there′s no alert report about it .Because the very small and mass-produced seed in siliques was dispersed by wind , the species can be potentially invasive . The result of our investigation indicated that there was a spreading trend of S. orientale in suburbs of Kunming, Central Yunnan . The biological characteristics and current status in Kunming were reported. We appealed to relevant government departments to make efforts to make a further investigation of its distribution and its invasion route . Assessment of its invasiveness in China is needed to eradicate it as early as possible to avoid its diffusion .     

云南消室叶蝉属一新种（同翅目：叶蝉科：隐脉叶蝉亚科）

记述消室叶蝉属Ｃhudania Distant １新种：丽江消室叶蝉Ｃhudania lijiangensis,sp.nov.。模式标本保存在贵州大学昆虫研究所。     

云南狭蚱属二新种(直翅目:短翼蚱科)

记述采自云南省普洱市南屏镇的直翅目Orthoptera、短翼蚱科Metrodoridae、狭蚱属Xistra Bolivar2新种:小叶狭蚱Xistra foliolata Liang,sp.nov.及小狭蚱Xistra parvula Liang,sp.nov.。模式标本保存于中山大学生物博物馆。     

中国伪遁蛛属Pseudopoda 2新种记述（蜘蛛目：异足蛛科）

报道了采白云南的伪遁蛛属Pseudopoda蜘蛛2新种：镇康伪遁蛛,新种Pseudopoda zhenkangensis sp．nov．和蝶形伪遁蛛,新种Prhopalocera sp．nov．．模式标本保存于大理学院生命科学与化学学院和中国林业科学研究院资源昆虫研究所。文中测量单位为mm。