氮素添加和CO2浓度升高对白羊草根际和非根际土壤水溶性有机碳、氮的影响

采用盆栽控制试验对黄土丘陵区白羊草在不同CO₂浓度(400和800 μmol·mol^-1)和施氮水平(0、2.5、5.0 g N·m^-2·a^-1)条件下根际和非根际土壤水溶性有机碳(DOC)和水溶性有机氮(DON)的变化特征进行研究.结果表明: CO₂浓度升高对白羊草根际和非根际土壤DOC、水溶性总氮(DTN)、DON、水溶性铵态氮(NH₄⁺-N)、水溶性硝态氮(NO₃^--N)含量均无显著影响.施氮显著提高了根际和非根际土壤DTN、NO₃^--N含量和根际土壤DON含量,显著降低了根际土壤DOC/DON.在各处理条件下,根际土壤DTN、NO₃^--N和DON含量均显著低于非根际土壤,根际土壤DOC/DON显著高于非根际土壤.短期CO₂浓度升高对黄土丘陵区土壤水溶性有机碳、氮含量无显著影响,而氮沉降的增加在一定程度上改善了土壤中水溶性氮素缺乏的状况,但并不足以满足植被对水溶性氮素的需求.     

氮素添加和CO2浓度升高对白羊草根际和非根际土壤水溶性有机碳、氮的影响

采用盆栽控制试验对黄土丘陵区白羊草在不同CO₂浓度(400和800 μmol·mol^-1)和施氮水平(0、2.5、5.0 g N·m^-2·a^-1)条件下根际和非根际土壤水溶性有机碳(DOC)和水溶性有机氮(DON)的变化特征进行研究.结果表明: CO₂浓度升高对白羊草根际和非根际土壤DOC、水溶性总氮(DTN)、DON、水溶性铵态氮(NH₄⁺-N)、水溶性硝态氮(NO₃^--N)含量均无显著影响.施氮显著提高了根际和非根际土壤DTN、NO₃^--N含量和根际土壤DON含量,显著降低了根际土壤DOC/DON.在各处理条件下,根际土壤DTN、NO₃^--N和DON含量均显著低于非根际土壤,根际土壤DOC/DON显著高于非根际土壤.短期CO₂浓度升高对黄土丘陵区土壤水溶性有机碳、氮含量无显著影响,而氮沉降的增加在一定程度上改善了土壤中水溶性氮素缺乏的状况,但并不足以满足植被对水溶性氮素的需求.     

植物种类对野芷湖湖岸带土壤反硝化作用的影响

反硝化作用是湖岸带有效去除氮素的关键氮循环过程,受到植物、土壤理化性质及微生物等因素的影响。为探究湖岸带不同植物对土壤反硝化作用的影响,通过调查测定野芷湖湖岸带9种常见植物的根际与非根际土壤的反硝化功能基因丰度和反硝化潜势,分析其与土壤理化性质的关系,阐明植物种类影响反硝化作用的机制。研究结果表明:(1)种植植物会改变湖岸带土壤的理化指标,植物根际土的硝态氮(NN)、铵态氮(AN)、总碳(TC)、总氮(TN)和可溶性有机碳(DOC)含量等显著高于非根际土,其中柳树、黄素馨、喜旱莲子草根际土的TC、DOC、TN、NN显著高于其他物种,桂树的根际和非根际土的pH均显著低于其他物种;(2)植物根际土的微生物反硝化功能基因(narG、napA、nirS、nirK和nosZ)丰度显著高于非根际土,其中柳树、黄素馨、喜旱莲子草根际土的基因丰度显著高于其他物种, pH、TN、NN和DOC等对反硝化功能基因丰度影响较大;(3)根际土的反硝化潜势显著高于非根际土,其中柳树、桂树、黄素馨根际土的反硝化潜势显著高于其他物种,黄素馨非根际土的反硝化潜势也较高,pH、DOC、TN以及napA、nirS、norB基因丰度等对土壤的反硝化潜势具有显著影响。本研究说明植物根际微环境更有利于反硝化微生物的生长与繁殖,湖岸带种植植物时可考虑不同的常绿和落叶乔木、灌木、草本植物的合理配置来提高湖岸带的氮素截留能力,以减少由过量氮素进入水生生态系统导致的面源污染。     

毛竹根际与非根际土壤重金属、理化性质及酶活性特征

于2014年2月至2015年1月在浙江省衢州市铅锌矿附近毛竹林地采集土样,对土壤重金属含量、pH以及土壤酶活性等的动态变化特征进行了研究。结果表明:根际土与非根际土的p H在1a中变化均不明显,且变化范围都处在4.50—4.85之间;非根际土的土壤含水率大于根际土,两者的变化幅度较大,最低值均出现在6月和10月;根际土中Zn和Cd的有效态含量远高于非根际土,而Pb的有效态含量则表现出非根际土大于根际土的特点;过氧化氢酶、磷酸酶、脲酶和脱氢酶在根际土中的活性明显高于非根际土;从土壤重金属有效态与土壤酶活性相关性来看,根际土中,除有效态Pb与过氧化氢酶呈显著负相关外,其余重金属元素有效态与土壤酶活性相关性均没有显著相关性,而在非根际土壤中,有效态Cd与过氧化氢酶呈极显著负相关,与脲酶呈显著负相关,Zn和Cd则与土壤酶活性之间没有显著相关性。综合研究结果表明,毛竹根际土壤质量总体上明显优于非根际土,这为毛竹今后作为植物修复的材料提供了一定的理论依据。     

Cadmium and zinc in plants and soil solutions from contaminated soils

In an experiment using ten heavy metal-contaminated soils from six European countries, soil solution was sampled by water displacement before and after the growth of radish. Concentrations of Cd, Zn and other elements in solution (K, Ca, Mg, Mn) generally decreased during plant growth, probably because of uptake by plants and the subsequent redistribution of ions onto soil exchange sites at lower ionic strength. Speciation analysis by a resin exchange method showed that most Cd and Zn in non-rhizosphere solutions was present as Cd2+ and Zn2+, respectively. The proportion of free ions was slightly lower in rhizosphere solutions, mainly due to an increase in dissolved organic carbon during plant growth. Solution pH increased during plant growth, although the bulk soil pH generally remained constant. Cd concentrations in leaves and tubers were more closely correlated with their total or free ionic concentrations in rhizosphere solutions (adjusted R2 0.90) than with their concentrations in soils (adj. R2 0.79). Cd concentrations in non-rhizosphere solutions were only poorly correlated with Cd concentrations in leaves and tubers. In contrast to Cd, there were no soil parameters that individually predicted Zn concentrations in leaves and tubers closely. However, multiple correlation analysis (including Zn concentrations in rhizosphere solutions and in bulk soils) closely predicted Zn concentrations in leaves and tubers (adj. R2 = 0.85 and 0.70, respectively). This suggests that the great variability among soils in the solubility of Zn affected the rate of release of Zn into solution, and thus Zn uptake. There was no such effect for Cd, for which solubility varied much less. Furthermore, the plants may have partly controlled Zn uptake, as they took up relatively less at high solution concentrations of Zn.Free ionic concentrations in soil solution did not predict concentrations of Cd or Zn in plants better than their total concentrations in solution. This suggests that with these soils, analysis of Cd and Zn speciation is of little practical importance when their bioavailability is assessed.     

A comparison of arsenic mobility in Phaseolus vulgaris, Mentha aquatica, and Pteris cretica rhizosphere

The ability of Phaseolus vulgaris, Mentha aquatica, and Pteris cretica to release arsenic (As) species from contaminated soil was tested in rhizobox experiments in three soils differing in their physicochemical parameters and total and mobile As concentration. Relatively low uptake of arsenic by P. vulgaris and M. aquatica resulted in very low and ambiguous changes in rhizosphere soil compared to bulk soil. However, there were observed differences in the distribution of the mobile As portion in soil to individual As species as affected by plant species and/or plantation conditions of these plants. Higher percentage of mobile arsenite in mint rhizosphere seems to be related to more reducing conditions during cultivation of these wetland plants. P. cretica planted in the soils containing between 36 and 1436 mg As kg⁻¹ was able to accumulate between 80 and 500 mg As kg⁻¹ in aboveground biomass. The extractable concentrations of As compounds in rhizosphere soil of P. cretica showed a clear depletion of arsenate (representing more than 90% of extractable arsenic) with the distance from plant roots. However, the As uptake mechanisms, as well as As transformation within hyperaccumulating fern plants, differ substantially from those in higher plants. Therefore the finding of suitable higher plant tolerant to the As soil contamination with good ability to accumulate As in aboveground biomass remains for the further research.     

Oxidation of pipecolic acid in soils and in rhizosphere soil of different plants

Soil respiration was proportional to its total carbon content. Maximum respiratory activity occurred in garden soil, followed in descending order by chernozem soil, brown soil, and sand. The oxidation of pipecolic acid, as studied by the Warburg manometric technique, in different rhizosphere soils from four crops 7, 13 and 20 days after planting as well as from one crop grown in different soils, was consistently in all cases faster than that by the corresponding non-rhizosphere soils. The curves of the rate of oxygen consumption during pipecolic acid oxidation, by garden soil (whether rhizosphere or non-rhizosphere soil) as well as by chernozem rhizosphere soil of different plants at the three stages of plant growth studied contained two peaks (two phases), whereas in non-rhizosphere chernozem soil as well as in brown soil and sand (whether affected or not affected by plant roots) only one peak was attained in the curves of the rate of oxygen uptake. The rapidity with which pipecolic acid was oxidized in the rhizosphere soil differed from plant to plant and at different phases of plant growth, and also with the type of soil used for plant growing. The extent of pipecolic acid oxidation after the first and second (if it occurred) phases did not differ in the different soils, both rhizosphere and non-rhizosphere soil, but the rate of oxygen uptake was higher in rhizosphere than in the corresponding non-rhizosphere soil. During the first phase, oxygen uptake accounted for slightly less than one-third of the total amount of oxygen required for complete oxidation of the added pipecolic acid. About two-thirds of that total amount were taken up during the both phases of oxidation.     

连香树人工林根系分泌物输入季节性变化及其驱动的根际微生物特性研究

根系分泌物介导的根际过程具有重要的生态学效应,但目前有关植物尤其是濒危珍稀植物根系分泌物及其介导的土壤生物地球化学循环过程的原位季节动态研究甚少。本文以阿坝州茂县大沟流域30年生的国家二级濒危保护植物连香树人工林为研究对象,于2014年4、7、9和12月利用原位收集装置对连香树根系分泌物进行原位收集;并同步分析了根际土壤微生物特性的变化。结果表明：（1）根系分泌物C和N分泌速率均呈现明显的季节动态变化,其中,夏季最高,冬季最低。（2）根际土壤微生物碳、氮及土壤酶活性显著高于非根际,表现出正的根际效应;根际效应也与根系分泌物输入表现出类似的季节动态规律,即夏季（7月）最高,而冬季（12月）最低。（3）进一步相关分析表明：根系分泌物分泌速率与土壤微生物碳、氮及土壤酶活性根际效应值呈线性正相关,表明根系分泌物输入是驱动根际微生物活性的重要因子,揭示连香树根系碳输入对根际土壤过程和功能的季节响应。未来研究应加强根系分泌物输入与土壤生物地球化学循环过程的偶联效应与机制研究。     

Bacterial diversity in the rhizosphere of Proteaceae species

The Cape Floral Kingdom is an area of unique plant biodiversity in South Africa with exceptional concentrations of rare and endemic species and experiencing drastic habitat loss. Here we present the first molecular study of the microbial diversity associated with the rhizosphere soil of endemic plants of the Proteaceae family (Leucospermum truncatulum and Leucadendron xanthoconus). Genomic DNA was extracted from L. truncatulum rhizosphere soil, L. xanthoconus rhizosphere and non-rhizosphere soil and used as a template for the polymerase chain reaction (PCR) amplification of the 16S ribosomal RNA gene (rDNA). Construction and sequencing of 16S rDNA libraries revealed a high level of biodiversity and led to the identification of several novel bacterial phylotypes. The bacterial community profiles were compared by 16S rDNA denaturing gradient gel electrophoresis (DGGE). Cluster analysis and biodiversity indices revealed that the rhizosphere soil samples were more similar to each other than to non-rhizosphere soil and the rhizosphere soil contained a bacterial diversity that was richer and more equitable compared with non-rhizosphere soil. A Chloroflexus and an Azospirillum genospecies were restricted to the L. xanthoconus rhizosphere soil and Stenotrophomonas genospecies was identified in all rhizosphere soil samples but was not present in the non-rhizosphere soil. Taxon-specific nested PCR and DGGE-identified differences between the Proteaceae plant rhizosphere soil with a Frankia genospecies restricted the L. truncatulum rhizosphere. Archaea-specific rDNA PCR, DGGE and DNA sequencing revealed that Crenarcheote genospecies were excluded from the plant rhizosphere soil and only present in non-rhizosphere soil.     

落叶松根际土壤磷的有效性研究

野外用剥落法采集落叶松(Larixgmelini)根际土与非根际土,分析P浓度的变化,结果表明,落叶松根际土与非根际土全P浓度无明显差异,但根际土有效P却明显高于非根际土.12年生时根际土有效P增加12.6%,40年生时增加23.4%,表明落叶松根际对土壤中的P具有活化作用.落叶松根际土无机P各组分与非根际土亦有差异,表现出根际土OP低于非根际土,AlP、FeP、CaP和NH4ClP则高于非根际土的趋势.落叶松根际土的pH低于非根际土,但落叶松根际并未发生明显酸化.落叶松根际有效P与pH变化相关不显著.     

Presence and oxidation of amino acids in rhizosphere and non-rhizosphere soil

Manometric studies were carried out on the respiratory activity of different rhizosphere and non-rhizosphere soils to follow quantitatively the over-all microbial activity in the rhizosphere soil as affected by the species and growth phase of plant. Oxygen consumption was distinctly greater in rhizosphere soils as compared to that in non-rhizosphere soils. The difference between oxygen consumption by rhizosphere and non-rhizosphere soils changed with the course of plant growth and it was not the same in different plants. This difference in oxygen consumption might be a measure of the amount of available oxidizable substrate in the rhizosphere. A rhizosphere sample had greater diversity as well as higher concentration of free amino acids than a non-rhizosphere sample of the same soil. Bacterial counts pointed to preferential stimulation in the rhizosphere of bacteria requiring individual amino acids. amino acids, such as glycine, alanine, asparsic acid of tyrosine were oxidized more rapidly in rhizosphere than in non-rhizosphere soil, but the extsent of oxidation for each of the amino acids studied did not differ. The amount of oxygen consumed during oxidation of alanine, aspartic acid or tyrosine was about one-half of the total amount necessary for complete oxidation. With glycine a higher extent of oxidation (60%) was observed. These extents of oxidation of glycine and aspartic acid did not change on investigation at two different phases of plant growth.     

普通鹿蹄草品质与根际和非根际土壤的关系

以野外调查和室内分析相结合,采用相关分析、主成分分析、回归分析、通径分析等多种分析方法,对秦岭太白山区不同生境下野生普通鹿蹄草有效成分(总黄酮、单宁、金丝桃苷、槲皮素和抗氧化活性(DPPHIC50)与其根际和非根际土壤性质的关系做了研究。结论如下:(1)根际土壤速效钾、pH和脲酶与单个有效成分含量之间呈现出显著或极显著作用,而非根际土壤速效钾与5种有效成分均达到显著或极显著正相关。(2)根际土壤速效钾、pH、转化酶和脲酶是影响普通鹿蹄草有效成分含量的主导因子,而非根际土壤有效氮、速效钾、pH和转化酶是综合影响其含量的主导因子。(3)根际土壤速效钾是影响普通鹿蹄草有效成分含量的主要决策因素,而pH、转化酶和脲酶是其主要限制因素;非根际土壤有效氮和速效钾是影响普通鹿蹄草有效成分含量的主要决策因素,而pH和转化酶是其主要限制因素。由此得出,普通鹿蹄草有效成分含量受根际和非根际多种土壤因子的综合影响,且土壤速效钾含量、pH、转化酶是影响其含量的共同因子。     

高寒草甸黄帚橐吾种群根际/非根际土壤可培养微生物群落特征

黄帚橐吾（Ligularia virgaurea）是高寒草甸常见的毒杂草,被认为是指示一个地区草地植被退化的重要物种,研究其根际/非根际土壤微生物在不同海拔梯度上的群落特征具有重要意义。以甘南州高寒草甸不同海拔梯度黄帚橐吾根际/非根际土壤可培养微生物为研究对象,采用稀释涂布平板法和最大可能数法（MPN）测定了土壤微生物的数量及土壤理化因子的变化。结果表明：细菌在微生物总数中占比最大,根际微生物数量随海拔升高呈先增加后减小的变化,非根际则表现为递增的趋势,微生物功能群在根际和非根际土壤中均逐渐增加;根际土壤的微生物和功能群数量均高于非根际土壤。RDA分析发现,土壤温度、有机碳、电导率、pH、全氮、全磷、速效氮及脲酶对根际/非根际土壤微生物数量及功能群变化影响较大。通径分析可知：根际土壤中,细菌和真菌受速效氮和有机碳影响较大,放线菌主要受土壤温度和电导率的影响;根际土壤固氮菌和氨化细菌决策系数速效氮 > 有机碳 > 全氮;根际和非根际土壤中硝化细菌的影响因子各不相同,根际土壤决策系数最大和最小分别为全磷和全氮,非根际则是pH和脲酶。     

宁南山区典型植物根际与非根际土壤微生物功能多样性

选择宁南山区9种典型植物的根际与非根际土壤为研究对象,采用Biolog方法对土壤微生物功能多样性进行了研究。结果表明:9种不同植物根际土壤与非根际土壤的微生物活性(AWCD)、微生物多样性指数和微生物均匀度指数均存在明显差异;除冰草外,其他各种植物的根际土壤的微生物活性AWCD、微生物多样性指数和微生物均匀度指数均比非根际土壤的高;9种典型植物根际土壤微生物主要碳源利用类型是羧酸类和氨基酸类,非根际土壤微生物主要碳源利用类型是羧酸类、胺类、氨基酸类;微生物活性、微生物多样性指数和微生物均匀度指数两两之间均达到了极显著相关,与土壤化学性质各指标之间均未达到显著相关水平。     

Green Manure and Overlapped Rhizosphere Effects on Pb Chemical Forms in Soil and Plant Uptake in Maize/Canola Intercrop Systems: A Rhizobox Study

To investigate the effects of single and mixed cultures of maize and canola, green manure addition, and distance from the rhizosphere on chemical forms of soil lead (Pb) and its uptake by plants, a greenhouse experiment was conducted using rhizobox systems. After plant harvest, the soils from different parts of the rhizoboxes were sampled and analyzed for dissolved organic carbon (DOC) concentrations and pH values. A sequential extraction procedure was also carried out to differentiate the chemical forms of Pb in the soil samples. Results showed that root activity significantly affected soil DOC concentration, pH and Pb chemical forms, while the effects generally ceased from the rhizosphere through the bulk soil. The pH value increased and DOC concentration decreased with distance from the roots. Green manure addition enhanced DOC concentration and exchangeable, carbonate-associated, and organic matter-bound Pb fractions. Green manure addition reduced the Pb concentration and uptake of maize but increased those of canola. The concentration of Pb in the roots of maize and canola was increased in intercropping compared with monocropping systems. The conclusion is that chemistry and bioavailability of soil Pb are interactively influenced by overlapped rhizospheres as compared to individual rhizospheres, use of green manure, and distance from the root surfaces.     

Phytoremediation of Total Petroleum Hydrocarbons From Highly Saline and Clay Soil Using Sorghum halepense (L.) Pers. and Aeluropus littoralis (Guna) Parl

This study evaluated the effects of native plants (Sorghum halepense and Aeluropus littoralis), total petroleum hydrocarbons (TPH) concentrations, and nutrients on the removal of TPHs from a highly saline clay soil. For a period of 180 days, rhizosphere microbial number, plant biomass, and residual TPHs were determined monthly. Results showed that TPH removal from soil in the rhizosphere was 13% higher than that in the control (unplanted soil). In addition, the number of heterotrophic bacteria in the rhizosphere and non-rhizosphere soil was 7.407 and 6.629 log₁₀CFU/g, respectively. The maximum TPH removal, microbial numbers, and plant biomass were measured in the treated soil, polluted with 0.86% (w/w) of TPH. The high clay and salinity of the experimental soil had a negative effect on the phytoremediation efficiency. Hence, it was necessary to improve the physicochemical properties of the soil to provide a good condition for plants and microbes, thereby increasing the phytoremediation efficiency.     

Influx and efflux of arsenic in cotton fields irrigated with arsenic-contaminated groundwater

Abstract

Samples of rhizosphere soil, groundwater used for drip irrigation, and mature cotton plants were collected from farms at Kuitun, Xinjiang and analyzed for their arsenic (As) levels to account for the influx and efflux of arsenic in the field. The pH value and the soil-grain-size distribution were found to correlate with the different levels of arsenic accumulated by cotton plants and arable soil layers. The principal component analysis (PCA) indicates that the arsenic level in soil is influenced by the silt content and the arsenic level in groundwater, while the arsenic level in plant roots is affected mainly by the silt content and the arsenic level in soil. As estimated, about 64.6?±?2.7?μg of arsenic was extracted from soil by one cotton plant, and more than 44% of it was stored in husks. About 0.8-2.6 mg As/(m² yr) was extracted by plants from soil according to the average dry weight of the plant and the field planting density, while 2.4–28.0?mg As/(m² yr) entered the soil based on the irrigated groundwater volume and the arsenic level in groundwater. Taking account of the cotton residues left after harvest, up to 85.8% of arsenic in the plants returned to the soil. After calculation, about 0.7–2.2?mg As/(m² yr) returned to the soil, and thus the actual net arsenic input to the soil could reach 2.3–27.6?mg As/(m² yr). Because of an obvious rise of the soil arsenic level in Kuitun, Xinjiang, and certain preventive measures should be taken to prevent the propagation of arsenic in the agricultural field.     

Production of organic acids by soil micro-organisms

Summary Micro-organisms of the rhizosphere soils in respect of four plant speciesviz sesbania, cowpea, sorghum, and finger millet and those of the non-rhizosphere soil (control) maintained under pot culture conditions were incubated for a fortnight in liquid media containing glucose and the relative amounts and nature of the organic acids produced were investigated by pH changes and chromatographic studies respectively. Micro-organisms with the rhizosphere of all the four plant species produced greater amounts of acids than those of the non-rhizosphere soil. Rhizosphere micro-organisms with legumes produced greater amounts of acids than those with non-legumes. However no qualitative differences were observed in the organic acids produced by the rhizosphere micro-organisms of the four plant species investigated as well as those of non-rhizosphere soil.     

短期CO2浓度升高和干旱胁迫对白羊草土壤碳氮和微生物根际效应的影响

采用人工气候室和盆栽控水试验研究黄土丘陵区典型草本植物白羊草在倍增CO₂浓度(800 μmol·mol^-1)下和充分供水(75%～80%的田间持水量)、轻度干旱胁迫(55%～60%的田间持水量)和重度干旱胁迫(35%～40%的田间持水量)下根际和非根际土壤碳氮含量和微生物群落结构及其根际效应.结果表明: CO₂浓度升高和干旱胁迫对白羊草根际和非根际土壤有机碳、全氮和水溶性有机碳(DOC)含量及其根际效应均无显著影响.轻度干旱胁迫下CO₂浓度升高显著促进了根际土壤水溶性有机氮(DON)的消耗,导致DOC/DON升高,提高了DON的负根际效应和DOC/DON的正根际效应.干旱胁迫和CO₂浓度升高对土壤总磷脂脂肪酸(总PLFA)和细菌PLFA的根际效应无显著影响.CO₂浓度升高条件下干旱胁迫显著提高了根际土壤G⁺/G^- PLFA,降低了非根际土壤G⁺/G^- PLFA,导致其根际效应显著提高,表明根际微生物群落由自养微生物群落向异养微生物群落的转变.     

短期CO2浓度升高和干旱胁迫对白羊草土壤碳氮和微生物根际效应的影响

采用人工气候室和盆栽控水试验研究黄土丘陵区典型草本植物白羊草在倍增CO₂浓度(800 μmol·mol^-1)下和充分供水(75%～80%的田间持水量)、轻度干旱胁迫(55%～60%的田间持水量)和重度干旱胁迫(35%～40%的田间持水量)下根际和非根际土壤碳氮含量和微生物群落结构及其根际效应.结果表明: CO₂浓度升高和干旱胁迫对白羊草根际和非根际土壤有机碳、全氮和水溶性有机碳(DOC)含量及其根际效应均无显著影响.轻度干旱胁迫下CO₂浓度升高显著促进了根际土壤水溶性有机氮(DON)的消耗,导致DOC/DON升高,提高了DON的负根际效应和DOC/DON的正根际效应.干旱胁迫和CO₂浓度升高对土壤总磷脂脂肪酸(总PLFA)和细菌PLFA的根际效应无显著影响.CO₂浓度升高条件下干旱胁迫显著提高了根际土壤G⁺/G^- PLFA,降低了非根际土壤G⁺/G^- PLFA,导致其根际效应显著提高,表明根际微生物群落由自养微生物群落向异养微生物群落的转变.