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1.
Zhong-Ji Qian BoMi Ryu Moon-Moo Kim Se-Kwon Kim 《Biotechnology and Bioprocess Engineering》2008,13(6):705-715
Seahorse, Hippocampus kuda (SH) a marine teleost fish, is well known not only for its special medicinal composition and used as one of the most famous
and expensive materials of traditional Chinese medicine. It was extracted with water (SHW), methanol (SHM), and ethanol (SHE),
respectively and evaluated by various antioxidant assays. The including reducing power, total antioxidant, DPPH radical scavenging,
hydroxyl radical scavenging, superoxide anion radical scavenging, alkyl radical scavenging, and protective effect on DNA damage
caused by hydroxyl radicals generated. Further, the ROS level was detected using a fluorescence probe, 2′,7′-dichlorofluorescin
diacetate (DCFH-DA), which could be converted to highly fluorescent dichlorofluorescein (DCF) with the presence of intracellular
ROS on mouse macrophages, RAW264.7 cell and inhibited myeloperoxidase (MPO) activity in human myeloid, HL60 cells, respectively.
Those various antioxidant activities were compared to standard antioxidants such as α-tocopherol. Among SHM exhibited the
highest antioxidant activity in linoleic acid system, effective reducing power, DPPH radical scavenging, hydroxyl radical
scavenging, superoxide radical scavenging, alkyl radical scavenging, inhibitory intracellular ROS, and inhibited MPO activity.
Furthermore, MTT assay showed no cytotoxicity on mouse macrophages cell (RAW264.7) and human cell lines (MRC-5, HL60, U937).
This antioxidant property depends on concentration and increasing with increased amount of extracts. The results obtained
in the present study indicated that the see horse (Hippocampus kuda Bleeker) is a potential source of natural antioxidant. 相似文献
2.
R. Matsukawa Z. Dubinsky E. Kishimoto K. Masaki Y. Masuda T. Takeuchi M. Chihara Y. Yamamoto E. Niki I. Karube 《Journal of applied phycology》1997,9(1):29-35
The inhibition of lipid peroxidation and radical scavenging effects were studied to evaluate the antioxidant activity for
extracts of 17 species of seaweed. The antioxidant effect was evaluated by determination of lipoxygenase activity and by α,
α-diphenyl-β-picrylhydrazyl (DPPH) decolorization. Lipoxygenase activity was depressed in the presence of aqueous and ethanol
extracts of 4 algal species; Sargassum species had the highest antioxidant activity of all the species examined. The ethanol
extracts of one Sargassum species showed competitive inhibition with the substrate. The same species also showed radical scavenging
activity in the DPPH decolorization test. Comparison of these results shows no relationship between enzyme inhibition and
radical scavenging activity.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
3.
Bilian Chen Wenlang You Jian Huang Ying Yu Weiping Chen 《World journal of microbiology & biotechnology》2010,26(5):833-840
The extracellular polysaccharide from Rhodella reticulata was separated from the culture medium followed by concentration and ethanol precipitation, and purified by anion exchange
chromatography on DEAE-Sepharose Fast Flow. This study compared the free radical-scavenging property and antioxidant activity
with various treatments of crude extracellular polysaccharides of R. reticulata. The results showed that both the crude extracellular polysaccharide and deproteinized crude extracellular polysaccharide
gave evidence of the free radical scavenging and antioxidant activity in a dose-dependent manner. The crude extracellular
polysaccharide exhibited higher free radical scavenging capacity and better antioxidant activity than the various treatments
of crude extracellular polysaccharide samples. The superoxide anion radical scavenging ability of various samples was significantly
higher compared to standard antioxidant (α-tocopherol). These results indicate that the extracellular polysaccharide of R. reticulata is a potent natural antioxidant. 相似文献
4.
In this study, an aqueous extract of leaves from Melothria maderaspatana was tested for in vitro antioxidant activity. Free radical scavenging assays, such as hydroxyl radical, hydrogen peroxide, superoxide anion radical
and 2,2-diphenyl-1-picryl hydrazyl (DPPH), 2,2’-azinobis-(3-ethyl-enzothiazoline-6-sulfonic acid) (ABTS) radical scavenging,
and reducing power assay, were studied. The extract effectively scavenged hydroxyl radical, hydrogen peroxide and superoxide
anion radicals. It also scavenged DPPH and ABTS radicals. Furthermore, it was found to have reducing power. All concentrations
of leaf extract exhibited free radical scavenging and antioxidant power, and the preventive effects were in a dose-dependent
manner. The antioxidant activities of the above were compared to standard antioxidants such as butylated hydroxytoluene (BHT),
ascorbic acid, and α-tocopherol. The results obtained in the present study indicate that the M. maderaspatana extract could be considered a potential source of natural antioxidant. 相似文献
5.
Hyon Hee Yoon 《Biotechnology and Bioprocess Engineering》1997,2(2):101-104
Lactic acid production from α-cellulose by simultaneous saccharification and fermentation (SSF) was studied. The cellulose
was converted in a batch SSF using cellulase enzyme Cytolase CL to produce glucose sugar andLactobacillus delbrueckii to ferment the glucose to lactic acid. The effects of temperature, pH, yeast extract loading, and lactic acid inhibition
were studied to determine the optimum conditions for the batch processing. Cellulose was converted efficiently to lactic acid,
and enzymatic hydrolysis was the rate controlling step in the SSF. The highest conversion rate was obtained at 46°C and pH
5.0. The observed yield of lactic acid from α-cellulose was 0.90 at 72 hours. The optimum pH of the SSF was coincident with
that of enzymatic hydrolysis. The optimum temperature of the SSF was chosen as the highest temperature the microorganism could
withstand. The optimum yeast extract loading was found to be 2.5 g/L. Lactic acid was observed to be inhibitory to the microorganisms’
activity. 相似文献
6.
Wei-Wei Zhang Xiao-Juan Duan Hai-Lan Huang Yi Zhang Bin-Gui Wang 《Journal of applied phycology》2007,19(2):97-108
The extracts obtained from 28 species of marine algae were evaluated for their antioxidant activity (AA) versus the positive
controls butylated hydroxytoluene (BHT), gallic acid (GA), and ascorbic acid (AscA). Most of the tested samples displayed
antioxidant activity to various degrees. Among them, the extract of Symphyocladia latiuscula exhibited the strongest AA, which was comparable to BHT, GA, and AscA in radical scavenging activity, as shown in the DPPH
(α,α-diphenyl-β-picrylhydrazyl) assay, and higher than those of the positive controls in β-carotene-linoleate assay system.
In addition, the ethyl acetate-soluble fraction isolated from the crude extract of S. latiuscula exhibited the highest antioxidant activity in both assay systems. This fraction was further fractionated into seven subfractions
(F1-F7) by vacuum liquid chromatography (VLC). F1 and F4 were found to be the most effective subfractions in scavenging DPPH
radical assay and in the β-carotene-linoleate assay, respectively. The total phenolic content (TPC) and reducing power (RP)
for all of the extracts, fractions, and subfractions (F1–F7) were also determined. The TPC of the 28 extracts ranged from
0.10 to 8.00 gallic acid equivalents (mg/g seaweed dry weight) while the RP ranged from 0.07 to 11.60 ascorbic acid equivalents
(mg·g−1 seaweed dry weight). Highly positive relationships between AA and TPC as well as between AA and RP were found for the extracts
and fractions, while for the subfractions F1–F7 only weak or no such relations were found. The results obtained from this
study indicate that further analysis is needed of those marine algal species that contain the most antioxidant activity in
order to identify the active principles. 相似文献
7.
Antioxidant activity of mycosporine-like amino acids isolated from three red macroalgae and one marine lichen 总被引:1,自引:0,他引:1
F. de la Coba J. Aguilera F. L. Figueroa M. V. de Gálvez E. Herrera 《Journal of applied phycology》2009,21(2):161-169
Several standard in vitro assays were performed in order to determine the potential antioxidant capabilities of purified aqueous
extracts of the mycosporine-like amino acids (MAAs), porphyra-334 plus shinorine (P-334 + SH), isolated from the red alga
Porphyra rosengurttii, asterina-330 plus palythine (AS-330 + PNE), from the red alga Gelidium corneum, shinorine (SH), from the red alga Ahnfeltiopsis devoniensis, and mycosporine -glycine (M-Gly), isolated from the marine lichen Lichina pygmaea. The scavenging potential of hydrosoluble radicals (ABTS+ decolorization method), the antioxidant activity in lipid medium (β-carotene/ linoleate bleaching method) and the scavenging
capacity of superoxide radicals (pyrogallol autooxidation assay) were evaluated. In terms of scavenging of hydrosoluble radicals,
the antioxidant activity of all MAAs studied was dose-dependent and it increased with the alkalinity of the medium (pH 6 to
8.5). M-Gly presented the highest activity in all pH tested; at pH 8.5 its IC50 was 8-fold that of L-ascorbic acid (L-ASC) followed by AS-330 + PNE while P-334 + SH and SH showed scarce activity of scavenging
of hydrosoluble free radicals. AS-330 + PNE showed high activity for inhibition of β-carotene oxidation relative to vitamin
E and superoxide radical scavenging whilst the activity of P-334 +SH and SH were moderate. According to these results, the
potential of MAAs in photoprotection can be considered high due to a double function: (1) UV chemical screening with high
efficiency for UVB and UVA regions of the solar spectrum, and (2) their antioxidant capacity. 相似文献
8.
Summary Botulinum neurotoxin (NT) is synthesized byClostridium botulinum in any of seven antigenically distinct forms, called types A through G. Protease(s) endogenous to the bacteria, or trypsin,
nicks the single chain protein to a dichain molecule which generally is more toxic. The conformation of dichain type A (nicked
by endogenous protease), single chain type E, and dichain type E NT (nicked by trypsin) have been determined using circular
dichroism (CD) and fluorescence spectroscopy. The high degree of ordered secondary structure (α helix 28%, β sheet 42%, total
70%) found in type A NT at pH 6.0 was similar to that found at pH 9.0 (α 22%, β 47%, total 69%). The secondary structure of
the single chain type E NT at pH 6.0 (α 18%, β 37%, total 55%) differed somewhat from these values at pH 9.0 (α 22%, β 43%,
total 65%). The dichain type E NT at pH 6.0 assumed a secondary structure (α 20%, β 47%, total 67%) more similar to that of
dichain type A than the single chain type E NT. Examination with the fluorogenic probe toluidine napthalene sulfonate revealed
that the hydrophobicity of the type A and E NTs were higher at pH 9.0 than at pH 6.0. Also, the hydrophobicity of the dichain
type E NT was higher than its precursor the single chain protein and appeared similar to that of the dichain type A NT. The
CD and fluorescence studies indicate that conversion of the single chain type E NT to the dichain form (i.e. nicking by trypsin)
induced changes in conformation. The ordered secondary structure (a + β contents) of botulinum NT, 70% for type A and 67%
for dichain type E, agree well with 65% of α + β contents of tetanus toxin [21] that is produced byClostridium tetani. 相似文献
9.
Toshiyuki Shibata Kanji Ishimaru Shigeo Kawaguchi Hiromichi Yoshikawa Yoichiro Hama 《Journal of applied phycology》2008,20(5):705-711
The antioxidant activities of brown algal phlorotannins were evaluated using the inhibition of phospholipid peroxidation in
the liposome system, and by determining radical scavenging activities against the superoxide anion and 1,1-diphenyl-2-picrylhydrazyl
(DPPH). Oligomers of phloroglucinol (1,3,5-trihydroxybenzene), eckol (a trimer), phlorofucofuroeckol A (a pentamer), dieckol
and 8,8′-bieckol (hexamers), isolated from the Laminarian brown algae Eisenia bicyclis, Ecklonia cava and Ecklonia kurome, showed potent inhibition of phospholipid peroxidation at 1 μM in the liposome system. The phlorotannins had significant
radical scavenging activities against the superoxide anion (50% effective concentration values: 6.5–8.4 μM) and DPPH (50%
effective concentration values: 12–26 μM), and were more effective compared to ascorbic acid and α-tocopherol. For the purpose
of using phlorotannins as functional food ingredients, the antioxidant activity of a complex of crude phlorotannins and soybean
protein was examined. The complex had a pronounced DPPH-radical scavenging activity. These results suggest that phlorotannins
are potent anti-inflammatory substances, and that the Laminariaceous brown algae, which are abundant in phlorotannins, may
be useful as a new functional foodstuff or supplement with anti-inflammatory activity. 相似文献
10.
The protein secondary structure and pigments' microenvironment in photosystem 1 (PS1) complexes were studied in the temperature
range of 25–80 °C using Fourier transform infrared (FT-IR) and circular dichroism (CD) spectroscopy, respectively. Quantitative
analysis of the component bands of the amide I band (1 700–1 600 cm−1) showed no significant change below 50 °C. However, apparent conformational changes occurred at 60 °C and further continued
at 70 and 80 °C accompanied with transitions of secondary structure mainly from α-helix to the β-sheet structures. CD analysis
demonstrated that the regular arrangement, viz. protein microenvironment of pigments of PS1 complexes, was destroyed by heat
treatment which might come from the changes of protein secondary structure of PS1. The CD signals at 645 nm contributed by
chlorophyll (Chl) b of light-harvesting complex 1 (LHC1) were easily destroyed at the beginning of heat treatment (25–60 °C). When temperature
reached 70 and 80 °C, the CD signals at 478 nm contributed mainly by Chl b of LHC1 and 498 nm contributed by carotenoids decreased most rapidly, indicating that LHC1 was more sensitive to high temperature
than core complexes. In addition, the oxygen uptake rate decreased by 90.81 % at 70 °C and was lost completely at 80 °C showing
that heat treatment damaged the regular function of PS1 complexes. This may be attributed to heat-induced changes of pigment
microenvironment and protein secondary structure, especially transmembrane α-helix located in PsaA/B of PS1. 相似文献
11.
The work was conducted with the purpose to evaluate antioxidant activity of Parmelia saxatilis (PS) by different analytical methods. Water and methanol were used as solvents and antioxidative effects were measured by
a ferric thiocyanate method (FTC) and thiobarbituric acid test (TBA). The antioxidant activity increased with the increasing
amount of extracts (from 50 to 250 μg) added to linoleic acid emulsion. The methanol extract of PS exhibited high antioxidative
activity that was not significantly (P < 0.05) different from α-tocopherol, while aqueous extracts of PS showed low antioxidative activity. Similar trends of antioxidant activity were observed
using either the FTC or TBA methods. Antioxidant activity, reducing power, free radical scavenging (DPPH·), superoxide anion radical scavenging, metal chelating and hydrogen peroxide scavenging activities of PS extracts showed
dose dependence and increased with concentration of PS extract. The results obtained in the present study indicate that the
PS might be a potential source of natural antioxidant. 相似文献
12.
Mayalen Zubia Daniel Robledo Yolanda Freile-Pelegrin 《Journal of applied phycology》2007,19(5):449-458
Extracts from 48 marine macroalgae species (17 Chlorophyta, 8 Phaeophyta and 23 Rhodophyta) from the coasts of Yucatan and
Quintana Roo (Mexico) were evaluated for antioxidant activity. The antioxidant activity was measured with the DPPH (2,2-diphenyl-1-picrylhydrasyl)
method, and the phenolic content of each extract were also evaluated. All species exhibited a DPPH radical scavenging activity,
and three species (Avrainvillea longicaulis, Chondria baileyana and Lobophora variegata) demonstrated great antioxidant potential with very low oxidation index EC50 (1.44 ± 0.01, 2.84 ± 0.07 and 0.32 ± 0.01 mg mL−1, respectively), significantly equivalent to EC50 of some commercial antioxidants such as α-tocopherol, ascorbic acid, BHA and BHT. Moreover, extracts of the most active species
exhibited reducing activities, superoxide anion radical scavenging and inhibition of lipid peroxidation. These results suggest
that some macroalgae from the Yucatan peninsula have a great antioxidant potential which could be considered for future applications
in medicine, food production or cosmetic industry. 相似文献
13.
Roberto Lo Scalzo Massimo Morassut Paolo Rapisarda 《Central European Journal of Biology》2012,7(1):146-158
The aim of the present study was the evaluation of the antioxidant content in phenolic and non-phenolic extracts of ten wine
samples, trying to elucidate the potential role of unusual antioxidant compounds. Samples of wines processed from red and
white grapes (Vitis vinifera L.), deprived of the volatile fraction at low temperature and buffered at physiological pH, were fractionated by C18 into two fractions: FR1 and FR2. Non-phenolics, such as tartaric, malic, lactic, and succinic acids; glucose; fructose; and
glycerin were mainly found in FR1, while polyphenols were present exclusively in FR2. Peroxyl radical quenching was assayed
by the ORAC method, while superoxide and hydroxyl radical scavenging activity were assayed by electron paramagnetic resonance.
In the ORAC and superoxide assays, most of the activity was found in FR2, while in hydroxyl radical assay, the activity was
found in FR1. Model solutions were used to attribute a role to the single compounds in the evaluation of wine’s ROS scavenging
capacity: the ORAC and superoxide anion scavenging effects were mainly attributed to the polyphenols, averaging 94.8%, with
some contribution from glycerin, particularly in white wines. Unexpectedly, the main chemical responsible for hydroxyl radical
scavenging activity was glycerin (56.1%), with the polyphenols scavenging at 18.1%. 相似文献
14.
Isolation and characterization of dihydromonacolin-MV from <Emphasis Type="Italic">Monascus purpureus</Emphasis> for antioxidant properties 总被引:4,自引:0,他引:4
Dhale MA Divakar S Kumar SU Vijayalakshmi G 《Applied microbiology and biotechnology》2007,73(5):1197-1202
The methanolic extract of Monascus purpureus cultivated by solid-state fermentation on rice showed strong 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity
and better yield as compared to other polarity based extracted fractions. It was selected for further purification of the
antioxidant. The activity-guided repeated fractionation of methanolic extract on a silica gel column chromatography yielded
a compound that exhibited strong antioxidant activity. Based on the spectroscopic analysis by UV, IR, 1H NMR, 13C NMR, 2D-HSQCT NMR, and MS, the antioxidant isolated was elucidated as a derivative of dihydromonacolin-K, where the ester
group is 2-methyl propionate, designated as dihydromonacolin-MV. The DPPH radical was significantly scavenged by the dihydromonacolin-MV
(IC50 20±1 μg ml−1). The dihydromonacolin-MV showed strong inhibition of lipid peroxidation in a liposome model with an IC50 value of 5.71±0.38 μg ml−1 and superoxide radical scavenging activity with an IC50 value of 163.97±2.68 μg ml−1. 相似文献
15.
Separation and properties of α-mannosidase and mannanase from the basidiomycetePhellinus abietis 总被引:1,自引:1,他引:0
Proteins of a crude enzyme preparation obtained from the cultivation medium of the basidiomycetePhellinus abietis were separated by gel filtration and ion-exchange chromatography. The preparation contained a minimum of three enzymes capable
of splitting α-d-mannosidic bonds: α-mannosidase, exomannanase, and endomannanase, which were separated. Some properties of the mannanase
complex of the crude enzyme preparation, and of a partially purified α-mannosidase were examined. The mannanase complex exhibited
two pH optima, its temperature optimum being at 46 °C The pH optimum of purified α-mannosidase was at pH 5.0, the temperature
optimum was at 60 °C; the enzyme had a relatively high heat stability. The Km of α-mannosidase forp-nitrophenyl α-d-mannopyranoside was 1.5 x 10−5 M. Pure α-mannosidase did not split mannan. 相似文献
16.
Siriluck Imjongjairak Khanok Ratanakhanokchai Chakrit Tachaapaikoon Patthra Pason Rattiya Waeonukul 《Bioscience, biotechnology, and biochemistry》2016,80(3):524-532
Sulfated polysaccharides (SPs) from Gracilaria fisheri of Thailand, which were extracted in low-temperature (25 °C) water showed the highest content of phenolic compounds compared with those extracted at high temperature (55 °C). Crude SP antioxidant activity was evaluated by measuring the DPPH free radical scavenging effect which is directly related to the level of phenolic compounds. The sulfate content, total sugar, and SPs yield were also directly related to the extraction temperature. All extracts contained galactose as a major monosaccharide. High antioxidant activity of crude SP, positively correlated with the phenolic compound contents (R2 = 0.996) contributed by the existence of sulfate groups and phenolic compounds. In purified SP, F1 fraction exhibited strong radical scavenging ability, but it was not significantly different compared to crude SP extracted at 25 °C. This indicated that the appropriate density and distribution of sulfate groups in the SP extract showed the best antioxidant activity. 相似文献
17.
Isabella Di Lernia Alessandra Morana Antonio Ottombrino Stefania Fusco Mosè Rossi M. De Rosa 《Extremophiles : life under extreme conditions》1998,2(4):409-416
Enzymes that convert starch and dextrins to α,α-trehalose and glucose were found in cell homogenates of the hyperthermophilic
acidophilic archaeon Sulfolobus shibatae DMS 5389. Three enzymes were purified and characterized. The first, the S. shibatae trehalosyl dextrin-forming enzyme (SsTDFE), transformed starch and dextrins to the corresponding trehalosyl derivatives with
an intramolecular transglycosylation process that converted the glucosidic linkage at the reducing end from α-1,4 to α-1,1.
The second, the S. shibatae trehalose-forming enzyme (SsTFE), hydrolyzed the α-1,4 linkage adjacent to the α-1,1 bond of trehalosyl dextrins, forming
trehalose and lower molecular weight dextrins. These two enzymes had molecular masses of 80 kDa and 65 kDa, respectively,
and showed the highest activities at pH 4.5. The apparent optimal temperature for activity was 70°C for SsTDFE and 85°C for
SsTFE. The third enzyme identified was an α-glycosidase (SsαGly), which catalyzed the hydrolysis of the α-1,4 glucosidic linkages
in starch and dextrins, releasing glucose in a stepwise manner from the nonreducing end of the polysaccharide chain. The enzyme
had a molecular mass of 313 kDa and showed the highest activity at pH 5.5 and at 85°C.
Received: October 29, 1997 / Accepted: April 29, 1998 相似文献
18.
Nack-Shick Choi Jae Jun Song Dong-Min Chung Young Jae Kim Pil Jae Maeng Seung-Ho Kim 《Journal of industrial microbiology & biotechnology》2009,36(3):417-426
A novel fibrinolytic enzyme (AJ) was purified from Staphylococcus sp. strain AJ screened from Korean salt-fermented Anchovy-jeot. Relative molecular weight of AJ was determined as 26 kDa by using SDS-PAGE and fibrin zymography. Based on a 2D gel, AJ
was found to consist of three active isoforms (pI 5.5–6.0) with the same N-terminal amino acid sequence. AJ exhibited optimum pH and temperature at 2.5–3.0 and 85°C, respectively.
AJ kept 85% of the initial activity after heating at 100°C for 20 min on the zymogram gel. The Michaelis constant (K
m) and K
cat values of AJ towards α-casein were 0.38 mM and 19.73 s−1, respectively. AJ cleaved the Aα-chain of fibrinogen but did not affect the Bβ- and γ-chains, indicating that it is an α-fibrinogenase.
The fibrinolytic activity was inhibited by diisopropyl fluorophosphate, indicating AJ is a serine protease. Interestingly,
AJ was very stable at acidic condition, SDS, and heat (100°C), whereas it was easily degraded at neutral and alkaline conditions.
In particular, AJ formed an active homo-dimer in the pH range from 7.0 to 8.0. To our knowledge, a similar combination of
acid and heat stability has not yet been reported for other fibrinolytic enzymes. 相似文献
19.
Bimal Kumar Ghimire Eun Soo Seong Eun Jung Goh Eun Young Kang Joung Kuk Ahn Chang Yeon Yu Ill Min Chung 《Plant Growth Regulation》2011,63(1):1-6
Codonopsis lanceolata Trautv (Companulaceae) is a folk medicine in Korea. To shift the content of tocopherol and enhance its antioxidant properties,
we overexpressed the γ-tocopherol methyltransferase (γ-tmt) gene in C. lanceolata. The antioxidant activity of methanolic crude extracts of the transgenic plants was compared to that of control plants using
the 1,1-diphenyl-2-picrylhydrazyl radical scavenging method, with α-tocopherol and butylated hydroxy toluene as standard antioxidants.
The antioxidant activity of the leaf and root extracts of transgenic plants was higher (IC 50 12–17.33 and 408–524 μg/ml, respectively) than that of control plant leaf and root extracts (18 and 529 μg/ml, respectively).
High-performance liquid chromatography analysis of phenolic compounds confirmed an increase in the levels of 12 major phenolic
acids and flavonoids in the leaf and root extracts of transgenic plants compared to control plants. We also found that the
rate of photosynthesis was 48% higher in transgenic plants than in control plants. Based on these results, we suggest that
increases in the α-tocopherol level in transgenic C. lanceolata plants may result in increases in the photosynthetic performance and antioxidant metabolism of these plants. 相似文献
20.
The effects of decoctions prepared from Antrodia camphorata on antioxidant, nitrite scavenging, and antitumor activities were investigated. Glutathione (GSH) production was 18.2 μM/g
of rat liver under the influence of a methanol extract, a result similar to with the effect of silymarin administration. GSH
peroxidase activity was about 2.0-fold higher than with silymarin administration. Superoxide dismutase activity was 18.9 U/mg
protein, about 2.5-fold higher than the control group. The hot water extract (1000 μg/mL) showed the highest nitrite scavenging
activity at 98.1%, similar to those observed with BHA and vitamin E. Among a variety of human cancer cell lines, when the
concentration of hot water extract was increased from 31 to 500 μg/mL, the viability of Hep3B cell was decreased from 100
to 60.7%. On the other hand, in the case of methanol extract, it was decreased from 98.4 to 10.0%. These results supported
the conclusion that the antioxidant, nitrite scavenging, and antitumor activities of this A. camphorata methanol extract indicate a potential source for the development of various health supplements and pharmaceutical and nutraceutical
applications. 相似文献