珠穆朗玛峰地区的锈菌

摘要:本文报告珠穆朗玛峰地区(中喜马拉雅)的锈菌计25属155种。所有标本系本文第一作者在1990年8月至10月在珠穆朗玛峰自然保护区(定结,吉隆,聂拉木和定日四县境内)进行科学考察时所采,保存在中国科学院微生物研究所真菌标本室。新种和中国新记录有:1.蓝钟花春孢锈菌Aecidium cyananthi J.Y. Zhuang sp. nov.2.坦登春孢锈菌Aecidium tandonii Mitter3.假风铃草鞘锈菌Coleosporium pseudocampanulae Kaneko, Kakishima & Ono4.坎宁安胶锈菌Gymnosporangium cunninghamianum Barclay5.无刺长栅锈菌Melampsoridium inerme Singh & Pandey6.巴克利多胞锈菌Phragmidium barclayi Dietel7.非洲短柄草柄锈菌戴维斯变种Puccinia brachypodii-phoenicoidis Guyot & Halencon var.davisii Cummins & H.C. Greene8.火把花生柄锈菌Puccinia colquhouniicola J.Y. Zhuang sp. nov.9.高山还羊参柄锈菌Puccinia crepidis-montanae Magnus10.迪里厄柄锈菌Puccinia durrieui Ono11.吉隆柄锈菌Puccinia gyirongensis J.Y. Zhuang sp. nov.12.苞茅柄锈菌Puccinia hyparrheniae Cummins13.淡灰柄锈菌Puccinia leucophaea H. Sydow & P. Sydow14.林克柄锈菌Puccinia linkii Klotzsch15.乳突柄锈菌Puccinia mammillata Schroeter16.帕氏柄锈菌Puccinia padwickii Cummins17.鞑靼茜草柄锈菌Puccinia rubiae-tataricae H. Sydow & P. Sydow18.风毛菊柄锈菌Puccinia saussureae Thuemen19.舌岩白菜柄锈菌Puccinia saxifragae-ciliatae Barclay20.葛缕子叶亮蛇床柄锈菌Puccinia selini-carvifoliae Savulescu21.西藏柄锈菌Puccinia thibetana J.Y. Zhuang sp. nov.22.脐景天柄锈菌Puccinia umbilici Guepin23.黄孢柄锈菌Puccinia xanthosperma H. Sydow & P. Sydow24.宫部膨痴锈菌Pucciniastrum miyabeanum Hiratsuka25.樱桃盖痂锈菌Thekopsora pseudo-cerasi Hiratsuka26.桑帕特轮孢锈菌Trochodium sampathense Thirumalachar27.间型拟夏孢锈菌Uredinopsis intermedia Kamei 新种有拉丁文描述,一些重要的种作了讨论。所有的种都列出了学名、寄主、采集地及标本号。     

青藏高原东缘的锈菌资料Ⅲ.禾本科植物柄锈菌补记

本文报告采自云南及青海的四种禾本科植物柄锈菌。其中鹅冠草Roegneria kamojiOhwi上的鹅冠草柄锈菌Puccinia roegneriae J.-y. Zhuang & S.-x. Wei为新种。纤毛鹅冠草Roegneria ciliaris(Trin.) Nevski上的日本猬草柄锈菌Puccinia asperellae-japonicae Hara,弓果黍Cyrtococcum patens(L.) A. Camus上的东方柄锈菌Puccinia orientalis(H.Syd., P. Syd. & Butl.) Arth. & Cumm.以及乱子草Muhlenbergia hugelii Trin.上的乱子草柄锈菌Puccinia schedonnardi Kell. & Swing.为我国新记录种。对新种作了拉丁文描述。对其余的三个新记录种都作了讨论。本研究引用的中国标本(包括新种的模式)保存在中国科学院微生物研究所真菌标本室。     

牛紏吴茱萸柄锈菌新种

本文描述了采自海南省琼中县牛紏吴茱萸Evodia trichotoma (Lour.) Pierre(芸香科)上的柄锈菌一新种——Puccinia evodiae-trichotomae J. -y. Zhuang。本种与巴布亚一新几内亚产的吴茱萸Evodia sp.上的另一种柄锈菌Puccinia evodiae Cumm.略近似,但冬孢子堆裸露呈垫状,而后者则深埋于病瘿中。冬孢子形状与大小和后者相仿,然而少数孢子可长达60μm,顶壁有时略增厚,颜色很浅或近几色。镜下可见一些孢子已萌发,推测可能属无眠冬孢型。芸香科植物上的柄锈菌目前仅知10种,本种特征与它们均不相同,故鉴定为新种。本新种模式标本保藏在中国科学院微生物研究所真菌标本室。     

青藏高原东缘的锈菌资料V.苔草属和姜属植物柄锈菌的增补

本文报告采自四川西部的三种柄锈菌新记录.它们是披针苔草Carex lanceolata Boott上的栗褐苔草柄锈菌Puccinia caricis-brunneae Dietel,苔草Carex sp.上的小堆柄锈菌Pucciniamicrosora Koernicke ex Fuckel以及阳荷Zingiber strialatum Dicls上的姜黄柄锈菌Puccinia curcumae T.S. Ramakrishnan & Sundaram。每个种有简要讨论并附线条图.标本保藏在中国科学院微生物研究所真菌标本室(HMAS)。     

樟科植物柄锈菌补记

本文报告了樟科植物上的柄锈菌计巧种。其中有4个新种:四川山胡椒柄锈Puccinia linderae-setchuenensis sp. nov.,黄丹木姜子柄锈Puccinia litseae-elongatae sp. nov., 赛楠柄锈Puccinia nothaphoebes sp. nov.和湘楠柄锈Puccinia phoebes- hunanensis sp. nov二新种模式标本保藏在中国科学院微生物研究所真菌标本室。研究了已知种的模式标本,并在文中作了简要讨论。文中附有种的检索表。     

玄参目植物柄锈菌的国产种

作者对中国科学院微生物研究所真菌标本室所藏的玄参目植物(爵床科,木犀科和玄参科)上的柄锈菌标本作了较详细的订正研究,共获得了12个种。其中藏玄参柄锈菌Puccinia oreosolenis J.-y. Zhuang为新种,产西藏定结(中喜马拉雅)高山冰缘地带。长尾婆婆纳柄锈菌Puccinia veronicae-longifoliae Savile为亚洲新记录,过去仅知分布芬兰和瑞典。本文对每个种均作了讨论并对各科植物上的种列出了检索表。     

毛茛目植物柄锈菌中国种的修订

作者对中国毛茛目植物(毛茛科,木通科和小檗科)上柄锈菌属的种进行了较详细的研究,对过去的错误报告和鉴定作了订正,共获得9个种,其中有3个新记录,即赛铁线莲柄锈菌Puccinia atragenes Hausm.,宽叶牛姆瓜柄锈菌Puccinia holboelliae-lat如liae Cumm.和鬼臼柄锈菌Puccinia podophylli Schw.。文中对每个种作了描述和讨论并附图和分种检索表。     

中国莎草科植物柄锈菌属的种

本文报告了中国莎草科植物的柄锈菌计33种.其中有2个新种,它们是双孔柄锈Puccinia biporosa sp. nov,和林氏苔草柄锈Puccinia caricis-lingii sp. nov.,均寄生于苔草属Carex.中国新记录种有13个.它们是大披针苔草柄锈Puccinia caricis-lanceolatae Y.Morimoto,云雾苔草柄锈Puccinia caricis-nubigenae Padwick ex AZmatullah Khan,疏毛苔草柄锈Puccinia caricis pilasae Miura ex Ito &Homma,假毒麦苔草柄锈Puccinia caricis-pseudololiaceae Homma,根状柄苔草柄锈Puecinia caricis rhizopodae Miura ex Ito &Homma,桑伯格苔草柄锈Puccinia caricis-thunbergii Homma,矮丛苔草生柄锈Puccinia humilicola Haslar,矮丛苔草柄锈Puccinia humilis Haslar,线形柄锈Puccinia lineariformis Syd,藻岩山柄锈Puccinia moiwensis Miura,奥皮茨柄锈Puccinia opiZii Bubak,林比苔草柄锈Puccinia yaramesuge Homma和珍珠茅生柄锈Puccinia scleriicola Arth..每个种均有形态描述并附图,有些种作了简要讨论,对过去的错误鉴定也作了订正.对苔草上的种还附有检索表.由于缺乏标本未能研究的种仅列出文献,寄主及产地.个别仅知春孢阶段的种作为暂定种列在附录中.本文所引证的标本均保藏在中国科学院微生物研究所真菌标本室.     

中国伞形科植物柄锈菌的分类研究

本文报告了中国寄生于伞形科植物上的柄锈菌19种,其中4种为中国新记录。它们是托马斯柄锈(Puccinia thomasii),贝加尔柄锈(Puccinia baikalensis),阿丽索娃柄锈(Puccinia alisovae)和山芹柄锈(Puccinia oreoselini),每个种均有形态描述并附图,有些种并附加简要讨论。由于缺少标本而未能研究的种仅在附录中列出文献、寄主和产地。     

青藏高原东缘的锈菌资料I.竹上的柄锈菌一新种

描述了采自四川西部山区竹上的柄锈菌一新种,即寄生在箭竹Sinarundinaria nitida(Mitf.) Nakai上的箭竹柄锈菌Puccinia sinarundinariae J-y. Zhuang & S.-x. Wei。模式标本保藏在中国科学院微生物研究所真菌标本室。     

Evolutionary relationships among aquatic anamorphs and teleomorphs: Lemonniera, Margaritispora, and Goniopila

The hypothesis that similar conidial morphologies in aquatic hyphomycetes are a result of convergent evolution was tested using molecular sequence data. Cladistic analyses were performed on partial sequences of 28S rDNA of seven species of Lemonniera, one species of Margaritispora and one species of Goniopila. Lemonniera has tetraradiate conidia with long arms, whereas Margaritispora and Goniopila have typically globose (isodiametric) conidia, with short conical protuberances in a stellate or quadrangular arrangement. Lemonniera and Margaritispora have phialidic conidiogenesis and both produce dark, minute sclerotia in culture whereas Goniopila has holoblastic conidiogenesis and does not produce sclerotia in culture. Goniopila produces a microconidial phialidic synanamorph in culture. All three genera have schizolytic conidial secession. Molecular analyses demonstrate that Lemonniera species are placed in two distinct clades: one within Leotiomycetes; the other within Pleosporales, Dothideomycetes. Margaritispora is placed with Lemonniera species within Leotiomycetes. Goniopila and Lemonniera pseudofloscula are placed within Dothideomycetes. No morphological character was entirely congruent with the molecular derived phylogeny. This suggests that for the group of species studied, conidial shape is not a reliable indicator of phylogeny but more likely the result of convergent evolution in response to the aquatic environment.     

中国黑粉菌属和利罗黑粉菌属

黑粉菌属是Roussel 1806年建立的,全世界记载有三百余种,主要寄生于禾本科,是经济作物及牧草的重要致病菌·长期以来,对黑粉菌的邢子使用过各种名称,如厚垣孢子,冬孢子及黑粉孢子等.本文采用黑粉孢子以区别锈菌的冬孢子. 芳’(1979)在《中国真菌总汇》中列出黑粉菌属五十种及一个变型.作者经过显微结构和超显微结构的研究,承认其中二十九种为正确名称,八种及一变型为异名,顶黑粉菌(Ustilago acrearus Berk.)由于错拼而被废弃.埃地黑粉菌(Ustilago emodensis Berk.)被转移至利罗粉菌属(Liroa).另有十一种黑粉菌因缺少标本留待今后订正.自1979年以后,杨信东(1983)增加黑粉菌属二种我国新纪录,K.范基和郭林(1986)描述一新种,四种新纪录.在本文中,作者描述一新种:鸢尾蒜黑粉(Ustilago ixiolirii Guo L) ,孢子堆生在蒴果内,不开裂,黑色,粉末状.黑粉孢子球形,近球形,稀椭圆形, 12.5-21×10-21μm,黑褐色,壁厚1-1.Sμm,纹饰脑状.是迄今生在石蒜科植物上唯一黑粉菌的种,其它几种黑粉菌均属条黑粉菌属.本文增加七种我国新纪录.共计四十九种,寄生于六科四十四属植物,主要是禾本科和蓼科.这仅是黑粉菌属研究的初步报告,在全国范围内大量采集黑粉菌标本后,作者相信会有更多新种和我国新纪录被发现.利罗黑粉菌属(Liroa)是从黑粉菌属(Ustaligo)分出的,此属为单种属.     

Probing the Drosophila retinal determination gene network in Tribolium (II): The Pax6 genes eyeless and twin of eyeless

The Pax6 genes eyeless (ey) and twin of eyeless (toy) are upstream regulators in the retinal determination gene network (RDGN), which instructs the formation of the adult eye primordium in Drosophila. Most animals possess a singleton Pax6 ortholog, but the dependence of eye development on Pax6 is widely conserved. A rare exception is given by the larval eyes of Drosophila, which develop independently of ey and toy. To obtain insight into the origin of differential larval and adult eye regulation, we studied the function of toy and ey in the red flour beetle Tribolium castaneum. We find that single and combinatorial knockdown of toy and ey affect larval eye development strongly but adult eye development only mildly in this primitive hemimetabolous species. Compound eye-loss, however, was provoked when ey and toy were RNAi-silenced in combination with the early retinal gene dachshund (dac). We propose that these data reflect a role of Pax6 during regional specification in the developing head and that the subsequent maintenance and growth of the adult eye primordium is regulated partly by redundant and partly by specific functions of toy, ey and dac in Tribolium. The results from embryonic knockdown and comparative protein sequence analysis lead us further to conclude that Tribolium represents an ancestral state of redundant control by ey and toy.     

IMI与K标本室中生于大戟科植物上链格孢属的种Ⅱ.大戟属,铁苋菜属和守宫木属上的种

本文系作者继大戟属(Euphorbia L.)、守宫木属(Sauropus B1.)和铁苋菜属(Acalypha L.)植物上链格孢属(Alternaria Nees)真菌研究(Zhang, 1995)之后,对生于大戟科(Euphorbiaceae)其它属植物上一些链格孢真菌种级分类单位评鉴结果的后续报道.内容包括:一个新种,巴豆生链格孢(A. croronicola T. Y.Zhang & J. C. David), Macrosporium compactum Cooke:对其模式标本(holotype)进行T订正、巴豆链格孢[A.crotonis kamal,Singh & Kumar]:提出关于新模式(neotype)标本的建议;对蓖麻链格孢[A. ricini(yoshii)Hansford]典型性状作了补充描述.此外,还在大戟科其它一些植物上检查到长极链格孢[A. longissima Deighton et MacGarvie]和细极链格孢[A. tenuissima(Nees ez Fr.) Wiltshire]。     

下延叉蕨和芽胞叉蕨配子体发育的研究

利用光学显微镜详细观察了叉蕨属(Tectaria)下延叉蕨(Tectaria decurrens)和芽胞叉蕨(T.fauriei)的配子体发育过程,记录了配子体各发育阶段的特征。结果表明:(1)下延叉蕨和芽胞叉蕨的孢子均为单裂缝,具周壁,由周壁形成纹饰,孢子极面观椭圆形,赤道面观豆形或肾形。(2)孢子萌发方式为向心型。(3)原叶体发育方式为三叉蕨型。(4)成熟原叶体心脏形,两翼向斜上方扩展。(5)均具单细胞和多细胞毛状体,在丝状体或片状体阶段出现。研究认为,从配子体发育角度看,叉蕨属是较进化的陆生真蕨类;毛状体的类型、位置和出现时间等特征在叉蕨属种间存在差异,可作为该属种间分类的特征。     

Hydroxylation of steroids by Fusarium oxysporum, Exophiala jeanselmei and Ceratocystis paradoxa

The potential of Fusarium oxysporum var. cubense UAMH 9013 to perform steroid biotransformations was reinvestigated using single phase and pulse feed conditions. The following natural steroids served as substrates: dehydroepiandrosterone (1), pregnenolone (2), testosterone (3), progesterone (4), cortisone (5), prednisone (6), estrone (7) and sarsasapogenin (8). The results showed the possible presence of C-7 and C-15 hydroxylase enzymes. This hypothesis was explored using three synthetic androstanes: androstane-3,17-dione (9), androsta-4,6-diene-3,17-dione (10) and 3α,5α-cycloandrost-6-en-17-one (11). These fermentations of non-natural steroids showed that C-7 hydroxylation was as a result of that position being allylic. The evidence also pointed towards the presence of a C-15 hydroxylase enzyme.The eleven steroids were also fed to Exophialajeanselmei var. lecanii-corni UAMH 8783. The results showed that the fungus appears to have very active 5α and 14α-hydroxylase enzymes, and is also capable of carrying out allylic oxidations.Ceratocystis paradoxa UAMH 8784 was grown in the presence of the above-mentioned steroids. The results showed that monooxygenases which effect allylic hydroxylation and Baeyer–Villiger rearrangement were active. However, redox reactions predominated.     

Prosthecium species with Stegonsporium anamorphs on Acer

Data from microscopic morphology, single-spore cultures, and DNA analyses of teleomorphs and anamorphs support the recognition of five species of Prosthecium with Stegonsporium anamorphs on Acer: P. acerinum sp. nov., the teleomorph of S. acerinum; P. acerophilum comb. nov., formerly known as Dictyoporthe acerophila; P. galeatum comb. nov., originally described as Massaria galeata; P. opalus sp. nov.; and P. pyriforme sp. nov., the teleomorph of S. pyriforme s. str. The morphology of both type specimens and freshly collected material was investigated. The teleomorphs have brown ellipsoidal ascospores with five distosepta and often a longitudinal distoseptum. The anamorphs of all species described here belong to Stegonsporium; their connection to the Prosthecium teleomorphs was demonstrated by morphology and DNA sequences of single spore cultures derived from both ascospores and conidia. The anamorphs and teleomorphs of all five Prosthecium species are described and illustrated by LM images, and a key to these species is provided. As perceived from this work, S. pyriforme is restricted to Europe and does not occur in North America, whereas S. acerinum is restricted to North America, not found in Europe. The host associations given in the literature are revised and evidence is provided that only A. opalus, A. pseudoplatanus, and A. saccharum are confirmed hosts of Prosthecium with Stegonsporium anamorphs. Molecular phylogenetic analyses of tef1, ITS rDNA, and partial nuLSU rDNA sequences confirm that the species with Stegonsporium anamorphs are closely related to P. ellipsosporum, the generic type species. Stilbospora macrosperma is confirmed as the anamorph of P. ellipsosporum by DNA data of single spore isolates obtained from both ascospores and conidia.     

The phylogenetic placement of Ostropales within Lecanoromycetes (Ascomycota) revisited

Results of molecular studies regarding the phylogenetic placement of the order Ostropales and related taxa within Lecanoromycetes were thus far inconclusive. Some analyses placed the order as sister to the rest of Lecanoromycetes, while others inferred a position nested within Lecanoromycetes. We assembled a data set of 101 species including sequences from nuLSU rDNA, mtSSU rDNA, and the nuclear protein-coding RPB1 for each species to examine the cause of incongruencies in previously published phylogenies. MP, minimum evolution, and Bayesian analyses were performed using the combined three-region data set and the single-gene data sets. The position of Ostropales nested in Lecanoromycetes is confirmed in all single-gene and concatenated analyses, and a placement as sister to the rest of Lecanoromycetes is significantly rejected using two independent methods of alternative topology testing. Acarosporales and related taxa (Acarosporaceae group) are basal in Lecanoromycetes. However, if the these basal taxa are excluded from the analyses, Ostropales appear to be sister to the rest of Lecanoromycetes, suggesting different ingroup rooting as the cause for deviating topologies in previously published phylogenies.     

New species of Phaeoramularia, Pseudocercospora and Stenella from Western Ghats of India

Four new species of the hyphomycete genera Phaeoramularia viz. Ph. caesalpinae, Pseudocercospora viz., Ps. tiliacearum, Stenella, viz. S. argyreiae and S. grewiae occurring on Caesalpinia bonducella Fleming (Caesalpiniaceae), Grewia sp. (Tiliaceae), Argyrea sp. Lour (Convolvulaceae) and Grewia sp. L. (Tiliaceae), respectively are described and illustrated here. All these fungi were collected from Western Ghats of India.