共查询到20条相似文献,搜索用时 46 毫秒
1.
David Popp Fujiet Koh Clement P. M. Scipion Umesh Ghoshdastider Akihiro Narita Kenneth C. Holmes Robert C. Robinson 《BioEssays : news and reviews in molecular, cellular and developmental biology》2018,40(4)
Structural biology has experienced several transformative technological advances in recent years. These include: development of extremely bright X‐ray sources (microfocus synchrotron beamlines and free electron lasers) and the use of electrons to extend protein crystallography to ever decreasing crystal sizes; and an increase in the resolution attainable by cryo‐electron microscopy. Here we discuss the use of these techniques in general terms and highlight their application for biological filament systems, an area that is severely underrepresented in atomic resolution structures. We assemble a model of a capped tropomyosin‐actin minifilament to demonstrate the utility of combining structures determined by different techniques. Finally, we survey the methods that attempt to transform high resolution structural biology into more physiological environments, such as the cell. Together these techniques promise a compelling decade for structural biology and, more importantly, they will provide exciting discoveries in understanding the designs and purposes of biological machines. 相似文献
2.
The field of membrane structural biology represents a fast-moving field with exciting developments including native nanodiscs that allow preparation of complexes of post-translationally modified proteins bound to biological lipids. This has led to conceptual advances including biological membrane:protein assemblies or “memteins” as the fundamental functional units of biological membranes. Tools including cryo-electron microscopy and X-ray crystallography are maturing such that it is becoming increasingly feasible to solve structures of large, multicomponent complexes, while complementary methods including nuclear magnetic resonance spectroscopy yield unique insights into interactions and dynamics. Challenges remain, including elucidating exactly how lipids and ligands are recognized at atomic resolution and transduce signals across asymmetric bilayers. In this special volume some of the latest thinking and methods are gathered through the analysis of a range of transmembrane targets. Ongoing work on areas including polymer design, protein labelling and microfluidic technologies will ensure continued progress on improving resolution and throughput, providing deeper understanding of this most important group of targets. 相似文献
3.
Hernández-Santoyo A 《Protein and peptide letters》2012,19(7):770-777
Macromolecular crystallography has been, for the last few decades, the main source of structural information of biological macromolecular systems and it is one of the most powerful techniques for the analysis of enzyme mechanisms and macromolecular interactions at the atomic level. In addition, it is also an extremely powerful tool for drug design. Recent technological and methodological developments in macromolecular X-ray crystallography have allowed solving structures that until recently were considered difficult or even impossible, such as structures at atomic or subatomic resolution or large macromolecular complexes and assemblies at low resolution. These developments have also helped to solve the 3D-structure of macromolecules from twin crystals. Recently, this technique complemented with cryo-electron microscopy and neutron crystallography has provided the structure of large macromolecular machines with great precision allowing understanding of the mechanisms of their function. 相似文献
4.
Molecular architecture of bacteriophage T4 总被引:4,自引:0,他引:4
Mesyanzhinov VV Leiman PG Kostyuchenko VA Kurochkina LP Miroshnikov KA Sykilinda NN Shneider MM 《Biochemistry. Biokhimii?a》2004,69(11):1190-1202
In studying bacteriophage T4—one of the basic models of molecular biology for several decades—there has come a Renaissance, and this virus is now actively used as object of structural biology. The structures of six proteins of the phage particle have recently been determined at atomic resolution by X-ray crystallography. Three-dimensional reconstruction of the infection device—one of the most complex multiprotein components—has been developed on the basis of cryo-electron microscopy images. The further study of bacteriophage T4 structure will allow a better understanding of the regulation of protein folding, assembly of biological structures, and also mechanisms of functioning of the complex biological molecular machines.Translated from Biokhimiya, Vol. 69, No. 11, 2004, pp. 1463–1476.Original Russian Text Copyright © 2004 by Mesyanzhinov, Leiman, Kostyuchenko, Kurochkina, Miroshnikov, Sykilinda, Shneider. 相似文献
5.
Kalko SG Chagoyen M Jiménez-Lozano N Verdaguer N Fita I Carazo JM 《European biophysics journal : EBJ》2000,29(6):457-462
Advances in structural biology are opening greater opportunities for understanding biological structures from the cellular
to the atomic level. Particularly promising are the links that can be established between the information provided by electron
microscopy and the atomic structures derived from X-ray crystallography and nuclear magnetic resonance spectroscopy. Combining
such different kinds of structural data can result in novel biological information on the interaction of biomolecules in large
supramolecular assemblies. As a consequence, the need to develop new databases in the field of structural biology that allow
for an integrated access to data from all the experimental techniques is becoming critical. Pilot studies performed in recent
years have already established a solid background as far as the basic information that an integrated macromolecular structure
database should contain, as well as the basic principles for integration. These efforts started in the context of the BioImage
project, and resulted in a first complete database prototype that provided a versatile platform for the linking of atomic
models or X-ray diffraction data with electron microscopy information. Analysis of the requirements needed to combine data
at different levels of resolution have resulted in sets of specifications that make possible the integration of all these
different types in the context of a web environment. The case of a structural study linking electron microscopy and X-ray
data, which is already contained within the BioImage data base and in the Protein Data Bank, is used here to illustrate the
current approach, while a general discussion highlights the urgent need for integrated databases.
Received: 26 January 2000 / Revised version: 15 May 2000 / Accepted: 15 May 2000 相似文献
6.
The combination of cryo-electron microscopy to study large biological assemblies at low resolution with crystallography to determine near atomic structures of assembly fragments is quickly expanding the horizon of structural biology. This technique can be used to advantage in the study of large structures that cannot be crystallized, to follow dynamic processes, and to "purify" samples by visual selection of particles. Factors affecting the quality of cryo-electron microscopy maps and limits of accuracy in fitting known structural fragments are discussed. 相似文献
7.
Peter B. Rosenthal 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2015,370(1666)
Elucidation of the structure of biological macromolecules and larger assemblies has been essential to understanding the roles they play in living processes. Methods for three-dimensional structure determination of biological assemblies from images recorded in the electron microscope were therefore a key development. In his paper published in Philosophical Transactions B in 1971, Crowther described new computational procedures applied to the first three-dimensional reconstruction of an icosahedral virus from images of virus particles preserved in negative stain. The method for determining the relative orientation of randomly oriented particles and combining their images for reconstruction exploited the high symmetry of the virus particle. Computational methods for image analysis have since been extended to include biological assemblies without symmetry. Further experimental advances, combined with image analysis, have led to the method of cryomicroscopy, which is now used by structural biologists to study the structure and dynamics of biological machines and assemblies in atomic detail. This commentary was written to celebrate the 350th anniversary of the journal Philosophical Transactions of the Royal Society. 相似文献
8.
Electron cryomicroscopy is a high-resolution imaging technique that is particularly appropriate for the structural determination of large macromolecular assemblies, which are difficult to study by X-ray crystallography or NMR spectroscopy. For some biological molecules that form two-dimensional crystals, the application of electron cryomicroscopy and image reconstruction can help elucidate structures at atomic resolution. In instances where crystals cannot be formed, atomic-resolution information can be obtained by combining high-resolution structures of individual components determined by X-ray crystallography or NMR with image-derived reconstructions at moderate resolution. This can provide unique and crucial information on the mechanisms of these complexes. Finally, image reconstructions can be used to augment X-ray studies by providing initial models that facilitate phasing of crystals of large macromolecular machines such as ribosomes and viruses. 相似文献
9.
Electron microscopy of high pressure frozen samples: bridging the gap between cellular ultrastructure and atomic resolution 总被引:3,自引:3,他引:0
Transmission electron microscopy has provided most of what is known about the ultrastructural organization of tissues, cells,
and organelles. Due to tremendous advances in crystallography and magnetic resonance imaging, almost any protein can now be
modeled at atomic resolution. To fully understand the workings of biological “nanomachines” it is necessary to obtain images
of intact macromolecular assemblies in situ. Although the resolution power of electron microscopes is on the atomic scale,
in biological samples artifacts introduced by aldehyde fixation, dehydration and staining, but also section thickness reduces
it to some nanometers. Cryofixation by high pressure freezing circumvents many of the artifacts since it allows vitrifying
biological samples of about 200 μm in thickness and immobilizes complex macromolecular assemblies in their native state in
situ. To exploit the perfect structural preservation of frozen hydrated sections, sophisticated instruments are needed, e.g.,
high voltage electron microscopes equipped with precise goniometers that work at low temperature and digital cameras of high
sensitivity and pixel number. With them, it is possible to generate high resolution tomograms, i.e., 3D views of subcellular
structures. This review describes theory and applications of the high pressure cryofixation methodology and compares its results
with those of conventional procedures. Moreover, recent findings will be discussed showing that molecular models of proteins
can be fitted into depicted organellar ultrastructure of images of frozen hydrated sections. High pressure freezing of tissue
is the base which may lead to precise models of macromolecular assemblies in situ, and thus to a better understanding of the
function of complex cellular structures. 相似文献
10.
Watching the components of photosynthetic bacterial membranes and their in situ organisation by atomic force microscopy 总被引:3,自引:0,他引:3
The atomic force microscope has developed into a powerful tool in structural biology allowing information to be acquired at submolecular resolution on the protruding structures of membrane proteins. It is now a complementary technique to X-ray crystallography and electron microscopy for structure determination of individual membrane proteins after extraction, purification and reconstitution into lipid bilayers. Moving on from the structures of individual components of biological membranes, atomic force microscopy has recently been demonstrated to be a unique tool to identify in situ the individual components of multi-protein assemblies and to study the supramolecular architecture of these components allowing the efficient performance of a complex biological function. Here, recent atomic force microscopy studies of native membranes of different photosynthetic bacteria with different polypeptide contents are reviewed. Technology, advantages, feasibilities, restrictions and limits of atomic force microscopy for the acquisition of highly resolved images of up to 10 A lateral resolution under native conditions are discussed. From a biological point of view, the new insights contributed by the images are analysed and discussed in the context of the strongly debated organisation of the interconnected network of membrane-associated chlorophyll-protein complexes composing the photosynthetic apparatus in different species of purple bacteria. 相似文献
11.
Simon Scheuring 《生物化学与生物物理学报:生物膜》2005,1712(2):109-127
The atomic force microscope has developed into a powerful tool in structural biology allowing information to be acquired at submolecular resolution on the protruding structures of membrane proteins. It is now a complementary technique to X-ray crystallography and electron microscopy for structure determination of individual membrane proteins after extraction, purification and reconstitution into lipid bilayers. Moving on from the structures of individual components of biological membranes, atomic force microscopy has recently been demonstrated to be a unique tool to identify in situ the individual components of multi-protein assemblies and to study the supramolecular architecture of these components allowing the efficient performance of a complex biological function.Here, recent atomic force microscopy studies of native membranes of different photosynthetic bacteria with different polypeptide contents are reviewed. Technology, advantages, feasibilities, restrictions and limits of atomic force microscopy for the acquisition of highly resolved images of up to 10 Å lateral resolution under native conditions are discussed. From a biological point of view, the new insights contributed by the images are analysed and discussed in the context of the strongly debated organisation of the interconnected network of membrane-associated chlorophyll-protein complexes composing the photosynthetic apparatus in different species of purple bacteria. 相似文献
12.
EU-OPENSCREEN is an academic research infrastructure initiative in Europe for enabling researchers in all life sciences to take advantage of chemical biology approaches to their projects. In a collaborative effort of national networks in 16 European countries, EU-OPENSCREEN will develop novel chemical compounds with external users to address questions in, among other fields, systems and network biology (directed and selective perturbation of signalling pathways), structural biology (compound-target interactions at atomic resolution), pharmacology (early drug discovery and toxicology) and plant biology (response of wild or crop plants to environmental and agricultural substances). EU-OPENSCREEN supports all stages of a tool development project, including assay adaptation, high-throughput screening and chemical optimisation of the ‘hit’ compounds. All tool compounds and data will be made available to the scientific community. EU-OPENSCREEN integrates high-capacity screening platforms throughout Europe, which share a rationally selected compound collection comprising up to 300,000 (commercial and proprietary compounds collected from European chemists). By testing systematically this chemical collection in hundreds of assays originating from very different biological themes, the screening process generates enormous amounts of information about the biological activities of the substances and thereby steadily enriches our understanding of how and where they act. 相似文献
13.
Detailed close-ups and the big picture of spliceosomes 总被引:1,自引:0,他引:1
Jurica MS 《Current opinion in structural biology》2008,18(3):315-320
14.
Super-resolution imaging by single-molecule localization (localization microscopy) provides the ability to unravel the structural organization of cells and the composition of biomolecular assemblies at a spatial resolution that is well below the diffraction limit approaching virtually molecular resolution. Constant improvements in fluorescent probes, efficient and specific labeling techniques as well as refined data analysis and interpretation strategies further improved localization microscopy. Today, it allows us to interrogate how the distribution and stoichiometry of interacting proteins in subcellular compartments and molecular machines accomplishes complex interconnected cellular processes. Thus, it exhibits potential to address fundamental questions of cell and developmental biology. Here, we briefly introduce the history, basic principles, and different localization microscopy methods with special focus on direct stochastic optical reconstruction microscopy (dSTORM) and summarize key developments and examples of two- and three-dimensional localization microscopy of the last 8 years. 相似文献
15.
Yahav T Maimon T Grossman E Dahan I Medalia O 《Current opinion in structural biology》2011,21(5):670-677
Visualization of cellular processes at a resolution of the individual protein should involve integrative and complementary approaches that can eventually draw realistic functional and cellular landscapes. Electron tomography of vitrified but otherwise unaltered cells emerges as a central method for three-dimensional reconstruction of cellular architecture at a resolution of 2-6 nm. While a combination of correlative light-based microscopy with cryo-electron tomography (cryo-ET) provides medium-resolution insight into pivotal cellular processes, fitting high-resolution structural approaches, for example, X-ray crystallography, into reconstructed macromolecular assemblies provides unprecedented information on native protein assemblies. Thus, cryo-ET bridges the resolution gap between cellular and structural biology. In this article, we focus on the study of eukaryotic cells and macromolecular complexes in a close-to-life-state. We discuss recent developments and structural findings enabling major strides to be made in understanding complex physiological functions. 相似文献
16.
Recent developments in electron cryomicroscopy and image analysis have made it a powerful tool to investigate the structure, assembly, and dynamics of biological supramolecular assemblies. The subjects of study now include a variety of biological samples that may be homogeneous or heterogeneous, symmetric or nonsymmetric. The combination of this technique with X-ray crystallography plays an increasingly important role in structural biology and provides unique structural information for understanding large, complex biological systems. Here we provide an overview of the technologies and specific applications to virus structure and function. 相似文献
17.
Sali A 《Structure (London, England : 1993)》2003,11(9):1043-1047
Recently, some 50 biologists and officials from government funding agencies met at the NIH campus in Bethesda, MD to explore the interdisciplinary science and organization of the emerging field of structural proteomics. Structural proteomics aims to discover most macromolecular complexes and characterize their three-dimensional structures and functional mechanisms in space and time. The goal seems daunting, but the consensus was that the prize would be commensurate with the effort invested, given the importance of molecular machines and functional networks in biology and medicine. Identification of assemblies and transient complexes combined with their structural and functional characterization will allow us to understand, control, design, and change the functioning of larger biological systems as well as to contribute to drug target discovery, lead discovery, and lead optimization for treatment of human disease. 相似文献
18.
The vibrational analysis of elastic models suggests that the essential motions of large biomolecular assemblies can be captured efficiently at an intermediate scale without requiring knowledge of the atomic structure. While prior work has established a theoretical foundation for this analysis, we demonstrate here on experimental electron microscopy maps that vibrational modes indeed describe functionally relevant movements of macromolecular machines. The clamp closure in bacterial RNA polymerase, the ratcheting of 30S and 50S subunits of the ribosome, and the dynamic flexibility of chaperonin CCT are extracted directly from single electron microscopy structures at 15-27 A resolution. The striking agreement of the presented results with experimentally observed motions suggests that the motion of the large scale machinery in the cell is surprisingly independent of detailed atomic interactions and can be quite reasonably described as a motion of elastic bodies. 相似文献
19.
Chiu W Baker ML Jiang W Dougherty M Schmid MF 《Structure (London, England : 1993)》2005,13(3):363-372
Advances in electron cryomicroscopy (cryo-EM) have made possible the structural determination of large biological machines in the resolution range of 6-9 angstroms. Rice dwarf virus and the acrosomal bundle represent two distinct types of machines amenable to cryo-EM investigations at subnanometer resolutions. However, calculating the density map is only the first step, and much analysis remains to extract structural insights and the mechanism of action in these machines. This paper will review the computational and visualization methodologies necessary for analysis (structure mining) of the computed cryo-EM maps of these machines. These steps include component segmentation, averaging based on local symmetry among components, density connectivity trace, incorporation of bioinformatics analysis, and fitting of high-resolution component data, if available. The consequences of these analyses can not only identify accurately some of the secondary structure elements of the molecular components in machines but also suggest structural mechanisms related to their biological functions. 相似文献
20.
Atomic force microscopy (AFM) is the type of scanning probe microscopy that is probably best adapted for imaging biological samples in physiological conditions with submolecular lateral and vertical resolution. In addition, AFM is a method of choice to study the mechanical unfolding of proteins or for cellular force spectroscopy. In spite of 28 years of successful use in biological sciences, AFM is far from enjoying the same popularity as electron and fluorescence microscopy. The advent of high-speed atomic force microscopy (HS-AFM), about 10 years ago, has provided unprecedented insights into the dynamics of membrane proteins and molecular machines from the single-molecule to the cellular level. HS-AFM imaging at nanometer-resolution and sub-second frame rate may open novel research fields depicting dynamic events at the single bio-molecule level. As such, HS-AFM is complementary to other structural and cellular biology techniques, and hopefully will gain acceptance from researchers from various fields. In this review we describe some of the most recent reports of dynamic bio-molecular imaging by HS-AFM, as well as the advent of high-speed force spectroscopy (HS-FS) for single protein unfolding. 相似文献