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The structure of the nuclear factor-kappaB protein-DNA complex varies with DNA-binding site sequence 总被引:3,自引:0,他引:3
Menetski JP 《The Journal of biological chemistry》2000,275(11):7619-7625
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Searching for DNA-protein interactions by lambda phage display 总被引:5,自引:0,他引:5
Cicchini C Ansuini H Amicone L Alonzi T Nicosia A Cortese R Tripodi M Luzzago A 《Journal of molecular biology》2002,322(4):697-706
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Identification of immunogenic antigens of Helicobacter pylori via the Escherichia coli hemolysin secretion system(1) 总被引:1,自引:0,他引:1
Spreng S Gentschev I Goebel W Mollenkopf H Eck M Müller-Hermelink HK Schmausser B 《FEMS microbiology letters》2000,186(2):251-256
We describe a new procedure allowing the generation and detection of immunogenic antigens from Helicobacter pylori via the hemolysin secretion apparatus of Escherichia coli. The gene (or gene fragment) encoding the H. pylori protein (or protein domain) is inserted in-frame into a residual portion of the hemolysin gene (hlyA), encoding the HlyA secretion signal (HlyA(s)). These fusion proteins are secreted efficiently by E. coli. This new approach allows the identification of immunodominant antigens by using sera derived from H. pylori-infected patients suffering from different gastroduodenal pathologies. Three immunodominant antigens bearing the ureB (urease B-subunit), flaA (flagellin A-subunit), and an unknown ORF (HP0888) encoding an E. coli FecE analogous protein fused to hlyA(s) were identified and characterized. 相似文献
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