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1.
O. V. Godukhin A. Yu. Budantsev T. G. Shchipakina V. E. Kondrat'ev 《Neurophysiology》1989,21(4):303-309
Involvement of the adenylate cyclase system in cholinergic modulation of synaptic transmission was investigated in area CA1 in rat hippocampal slices. Microiontophoretic application of acetylcholine as well as addition of carbachol to the superfusate or of tolbutamide (a cAMP-dependent protein kinase inhibitor) depressed transmission at synapses formed by Schaffer collaterals and commissural fibers with dendrites of pyramidal cells belonging to hippocampal area CA1. Both numbers of free quanta of neurotransmitter and the likelihood of transmitter release decreased following carbachol action. Atropine suppressed the inhibitory action of carbachol on synaptic transmission. Dibutyryl cAMP and forskolin increased the amplitude of synaptic potentials and suppressed, either partially or in full, the inhibitory effects of cholinomimetics on synaptic potentials. It was concluded that cholinomimetics and activators of the adenylate cyclase system exert opposing effects on neurotransmission at synapses formed between Schaffer collaterals/commissural fibers and dendrites of pyramidal neurons belonging to hippocampal area CA1.Institute of Biophysics, Academy of Sciences of the USSR, Pushchino. Translated from Neirofiziologiya, Vol. 21, No. 4, pp. 435–442, July–August, 1989. 相似文献
2.
Unit responses of the lateral geniculate bodies (LGB) to polarization of the cells through the recording microelectrode were investigated in acute experiments on cats anesthetized with Nembutal. Under the influence of anodic polarization the firing rate of the LGB cells clearly increased. Complete adaptation of the cells to the polarizing current was not observed during the time intervals investigated (5–10 min). Cathodic polarization by a current of 5–50 nA induced inhibitory effects; neurons with a single type of spontaneous activity under these circumstances generated volleys of 2–5 spikes. Off-responses were recorded in 75–85% of neurons. It is postulated that complex changes in unit activity produced by polarization may be due to the structural characteristics of the functional connections of the LGB neurons investigated. The change to grouped activity on the part of many of the neurons under the influence of cathodic polarization is evidently explained by the specific functional pattern of the synaptic system of the LGB cells.Institute of Experimental Medicine, Academy of Medical Sciences of the USSR, Leningrad. Translated from Neirofiziologiya, Vol. 4, No. 2, pp. 130–140, March–April, 1972. 相似文献
3.
The soundness of hypotheses interpreting the mechanism of formation of background activity (BA) generated by the neurons of the rabbit superior cervical ganglion was analyzed, taking into account the statistical characteristics of this activity. These neurons are supplied with a multiple synapic input. According to the random hypothesis, the BA action potentials in the ganglion neurons result from occassional coincidence of the spikes arriving through independent presynaptic inputs. Our calculations showed that the frequency of BA action potentials observed in the experiments is too high to be explained by the random hypothesis and needs synchronization of firing in different presynaptic neurons, rather than their independent firing.Neirofiziologiya/Neurophysiology, Vol. 28, No. 1, pp. 47–52, January–February, 1996. 相似文献
4.
Deborah Prè Michael W. Nestor Andrew A. Sproul Samson Jacob Peter Koppensteiner Vorapin Chinchalongporn Matthew Zimmer Ai Yamamoto Scott A. Noggle Ottavio Arancio 《PloS one》2014,9(7)
Many protocols have been designed to differentiate human embryonic stem cells (ESCs) and human induced pluripotent stem cells (iPSCs) into neurons. Despite the relevance of electrophysiological properties for proper neuronal function, little is known about the evolution over time of important neuronal electrophysiological parameters in iPSC-derived neurons. Yet, understanding the development of basic electrophysiological characteristics of iPSC-derived neurons is critical for evaluating their usefulness in basic and translational research. Therefore, we analyzed the basic electrophysiological parameters of forebrain neurons differentiated from human iPSCs, from day 31 to day 55 after the initiation of neuronal differentiation. We assayed the developmental progression of various properties, including resting membrane potential, action potential, sodium and potassium channel currents, somatic calcium transients and synaptic activity. During the maturation of iPSC-derived neurons, the resting membrane potential became more negative, the expression of voltage-gated sodium channels increased, the membrane became capable of generating action potentials following adequate depolarization and, at day 48–55, 50% of the cells were capable of firing action potentials in response to a prolonged depolarizing current step, of which 30% produced multiple action potentials. The percentage of cells exhibiting miniature excitatory post-synaptic currents increased over time with a significant increase in their frequency and amplitude. These changes were associated with an increase of Ca2+ transient frequency. Co-culturing iPSC-derived neurons with mouse glial cells enhanced the development of electrophysiological parameters as compared to pure iPSC-derived neuronal cultures. This study demonstrates the importance of properly evaluating the electrophysiological status of the newly generated neurons when using stem cell technology, as electrophysiological properties of iPSC-derived neurons mature over time. 相似文献
5.
A. Ya. Ivanov 《Neurophysiology》1987,19(5):495-501
Tonic activity in rabbit superior cervical ganglion neurons was investigated using intracellular recording techniques as well as changes produced when the animal breathed a gaseous mix with a raised CO2 level. The test neurons were divided into three groups depending on the pattern of their tonic activity and reflex change. Action potentials were produced by the activity of dominant and accessory preganglionic inputs in the firing pattern of all neuronal groups, implying the existence of other types of inputs into the neurons innervating different organs. Having analyzed changes in action potential rate and EPSP in the tonic activity of neurons from different groups, it was presumed that preganglionic fibers with a similar activity pattern converge on the majority of neurons in each group.A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 19, No. 5, pp. 665–672, September–October, 1987. 相似文献
6.
The effects of subiculum stimulation were investigated in 80 antidromically identified hypothalamic supraoptic neurons in lactating rats. Inhibition manifesting as suppression of antidromic action potentials (or of their somatodendritic component) was revealed in 26% of cells, induced by applying conditioned and test stimuli to the subiculum and neurohypophysial stalk. In some instances inhibition arose following a latency of 5–25 msec after each subicular stimulus and lasted only briefly; it set in gradually in other cases, leading to stable long-term changes in the excitability of neurosecretory cells. No activation was produced by this stimulation. It is deduced that subicular inhibitory inputs follow different patterns, thus reflecting morphological organizational aspects of synaptic inhibitory inputs to neurosecretory cells.A. A. Ukhtomskii, Institute of Physiology, A. A. Zhdanov State University, Leningrad. Translated from Neirofiziologiya, Vol. 20, No. 4, pp. 431–437, July–August, 1988. 相似文献
7.
We report on factors affecting the spontaneous firing pattern of the identified serotonin-containing Retzius neurons of the medicinal leech. Increased firing activity induced by intracellular current injection is followed by a ‘post-stimulus-depression’ (PSD) without spiking for up to 23 s. PSD duration depends both on the duration and the amplitude of the injected current and correlates inversely with the spontaneous spiking activity. In contrast to serotonin-containing neurons in mammals, serotonin release from the Retzius cells presumably does not mediate the observed spike suppression in a self-inhibitory manner since robust PSD persists after synaptic isolation. Moreover, single additional spikes elicited at specific delays after spontaneously occurring action potentials are sufficient to significantly alter the firing pattern. Since sub-threshold current injections do not affect the ongoing spiking pattern and PSD persists in synaptically isolated preparations our data suggest that PSD reflects an endogenous and ‘spike-dependent’ mechanism controlling the spiking activity of Retzius cells in a use-dependent way. 相似文献
8.
Neuronal activity was recorded in surviving hippocampal and medial preoptic thalamic slices from guinea pigs using extracellular techniques during thermal changes. Rate of generating action potentials changed in seven of the 19 hypothalamic cells tested once a threshold temperature of 36–38°C had been reached. Above this range, activity in these neurons was temperature dependent. It is suggested that these neurons form a sensory element in the system controlling brain temperature over a narrow (1–2°C) range. In the hippocampus (the control structure), pyramidal layer cells were insensitive to temperatures in the 32–40°C range.Institute of Physiology, Academy of Sciences of the Byelorussian SSR, Minsk. Translated from Neirofiziologiya, Vol. 21, No. 3, pp. 358–365, May–June, 1989. 相似文献
9.
Modulation of synaptic events by heavy metals in the central nervous system of mollusks 总被引:1,自引:0,他引:1
Summary 1. The effects of heavy metals (Pb2+, Hg2+, and Zn2+) on synaptic transmission in the identified neural network ofHelix pomatia L. andLymnaea stagnalis L. (Gastropoda, Mollusca) were studied, with investigation of effects on inputs and outputs as wells as on interneuronal connections.2. The sensory input running from the cardiorenal system to the central nervous system and the synaptic connections between central neurons were affected by heavy metals.3. Lead and mercury (10–5–10–3
M) eliminated first the inhibitory, then the excitatory inputs running from the heart to central neurons. At the onset of action lead increased the amplitude of the excitatory postsynaptic potentials, but blockade of sensory information transfer occurred after 10–20 min of treatment.4. The monosynaptic connections between identified interneurons were inhibited by lead and mercury but not by zinc. Motoneurons were found to be less sensitive to heavy metal treatment than interneurons or sensory pathways.5. The treatment with Pb2+ and Hg2+ often elicited pacemaker and bursting-type firing in central neurons, accompanied by disconnection of synaptic pathways, manifested by insensitivity to sensory synaptic influences.6. Zn2+ treatment also sometimes induced pacemaker activity and burst firing but did not cause disconnection of the synaptic transmission between interneurons.7. A network analysis of heavy metal effects can be a useful tool in understanding the connection between their cellular and their behavioral modulatory influences. 相似文献
10.
L. A. Tatarchenko 《Neurophysiology》1989,21(2):193-200
Intracellular recording techniques were used to record electrical response from neurons of the rabbit (isolated) superior cervical ganglion to single stimuli applied to bundles of preganglionic fibers as well as tonic electrical neuronal activity in this ganglion during acute experiments in situ. A review of the findings obtained confirms that neurons of the ganglion receive preganglionic synaptic inputs of two types, the first of a single pattern, formed by a preganglionic fiber; excitatory action of the latter on ganglion units suffices to induce postsynaptic action potentials (AP I) and the second of a multiple pattern, formed by several preganglionic fibers with relatively faint excitatory action, capable of evoking postsynaptic action potentials (AP II) only when excited in unison. Interspike intervals for AP I and AP II in tonic neuronal activity conformed to a normal distribution and a random distribution pattern respectively.A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 21, No. 2, pp. 252–261, March–April, 1989. 相似文献
11.
N. A. Otmakhov 《Neurophysiology》1986,18(1):80-88
The activity of 67 nonpyramidal neurons from the str. radiatum moleculare (NSRM) and 8 presumed interneurons of str. orienspyramidale (NSOP) was recorded in guinea pig hippocampal slices. In contrast with the high frequency grouped discharges characteristic of NSOP, NSRM had low frequency background activity consisting of a single action potential (77%) and grouped spikes (23%). The spontaneous firing rate of neurons of the radial layer decreased with increasing distance from the pyramidal layer. NSRM responded with one or two action potentials rather than a burst of spikes to electrical stimulation of the dentate fascia. The threshold of response for NOSP was lower, while that of NSRM was the same on average but was significantly higher than for NSOP on a number of occasions. The response of both types of neuron was usually accompanied by an inhibitory pause. During induced epileptoid activity single or grouped discharges arose in neurons of both types concurrently with synchronized bursts in the pyramidal cells. It is postulated that NSOP exert an excitatory effect on NSRM.Institute of Biophysics, Academy of Sciences of the USSR, Puschino-on-Oka, Moscow Province, Translated from Neirofiziologiya, Vol. 18, No. 1, pp. 99–108, January–February, 1986. 相似文献
12.
Synaptic response to single (2 Hz) and regular (30–50 Hz) stimuli applied to the pontine inhibitory site were recorded in decerebrate cats. A change to regular stimulation was usually accompanied by a rise in the firing index of synaptic discharges and raised amplitude of inhibitory and (to a lesser extent) excitatory postsynaptic potentials. Suppression of background spike activity was observed in some neurons. It was deduced that frequency potentiation makes a considerable contribution to the functional effect of stimulating the inhibitory site, i.e., terminating evoked locomotion.Institute for Information Transmission Studies, Academy of Sciences of the USSR, Moscow. Translated from Neirofiziologiya, Vol. 20, No. 2, pp. 172–180, March–April, 1988. 相似文献
13.
A small modification to a voltage-clamp set-up for studying isolated neurons, and the use of simple hippocampal slices allowed stable recording of excitatory postsynaptic currents (EPSCs) that were evoked by stimulating the Shaffer's collaterals of individual CA1 pyramidal neurons. With the developed method EPSCs and focal extracellular potentials could be recorded simultaneously. It was confirmed that the EPSC consists of two components that are mediated via N-methyl-D-aspartate (NMDA)- and non-NMDA-receptors. The effects of different blockers of these receptors on the postsynaptic current were investigated, as were the effects of adenosine, which, depending on its concentration, could either depress or potentiate the synaptic transmission.A. A. Bogomolets Institute of Physiology, Ukrainian Academy of Sciences, Kiev. Translated from Neirofiziologiya, Vol. 23, No. 6, pp. 731–738, November–December, 1991. 相似文献
14.
N. A. Otmakhov 《Neurophysiology》1988,20(1):52-60
Investigations were performed on the effects of acetylcholine (ACh), norepinephrine (NE), 5-hydroxytryptamine (5-HT), and -aminobutyric acid (GABA) on the background firing of the three following groups of field CA3 neurons in guinea pig hippocampal slices: nonpyramidal neurons of the stratum radiatum moleculare (NSR), stratum pyramidale cells with single spike discharges (SD units), and those with complex discharge patterns (CD units) within the same layer. The action of ACh and NE on presumed interneurons of the pyramidal layer (IPL) was also investigated; CD units were found to differ from the remaining groups, which reacted similarly to the transmitters tested. It was shown that NE, 5-HT, and GABA inhibited the activity of CD cells, while ACh produced inhibitory-activating response in 50% of these units. Both NE and ACh exerted a monophasic activating effect on NSR, ISP, and SD, however, while 5-HT and GABA induced activation in a proportion of NSR and SD cells, as well as inhibitory response. The excitatory effects produced by ACh, NE, and 5-HT on NSR persisted during blockade of synaptic transmission, indicating that associated afferent fibers may be acting directly on these cells.Institute of Biological Physics, Academy of Sciences of the USSR, Pushchino-on-Oka. Translated from Neirofiziologiya, Vol. 20, No. 1, pp. 64–74, January–February, 1988. 相似文献
15.
G. G. Arakelov 《Neurophysiology》1973,5(1):71-76
Intracellular microelectrode recordings from neurons ofHelix pomatia revealed several local zones of action potential generation both on the soma and on some of the branches of the neurons. Under certain conditions the activity of individual loci of the neuron membrane was synchronized to produce a normal action potential. It is suggested that the somatic membrane of neurons is heterogeneous in structure and consists of separate loci of an electrically excitable membrane, incorporating active and latent pacemaker zones. Neurons ofH. pomatia are characterized by two types of action potential with different triggering mechanisms: one (synaptic) type is generated under the influence of the EPSP, the other (pacemaker) arises through activation of endogenous factors for the neuron (pacemaker potentials). The interaction between synaptic and pacemaker potentials during integrative activity of the neuron is discussed.M. V. Lomonosov Moscow State University. Translated from Neirofiziologiya, Vol. 5, No. 1, pp. 88–94, January–February, 1973. 相似文献
16.
Adenosine and the adenosine 5'-phosphates (5'-AMP, 5'-ADP and 5'-ATP) depress the spontaneous firing of cerebral cortical neurons. In this study adenosine analogs, adenosine transport blockers and adenosine deaminase inhibitors have been used to gain further insight into the nature of the adenosine receptor and the likely routes of metabolism of extracellularly released adenosine. The firing rate of cortical neurons, including identified corticospinal neurons, was depressed by 2-substituted derivatives of adenosine. 2-Halogenated derivatives of adenosine were potent depressors while 2-aminoadenosine and 2-hydroxyadenosine (crotonoside) were slightly less potent than adenosine. The α,β-methylene isosteres of 5'-ADP and 5'-ATP were almost devoid of agonist activity while the β,γ-methylene analog was an active agonist. This suggests that ADP and ATP must be converted to AMP or possibly adenosine before they can activate the adenosine receptor. 2'-, 3'- and 5'- deoxyadenosine depressed spontaneous firing without antagonizing the effect of adenosine. Adenosine deaminase inhibitors, deoxycoformycin and -9-(2-hydroxy-3-nonyl) adenine had potent, long lasting depressant actions on the spontaneous firing of cortical neurons and concurrently potentiated the actions of adenosine or 5'-AMP. Inhibitors of adenosine transport, papaverine and 2-hydroxy-5-nitrobenzylthioguanosine, prolonged the duration of action of adenosine and 5'-AMP. Intracellular recordings show that 5'-AMP hyperpolarizes cerebral cortical neurons and suppresses spontaneous and evoked excitatory postsynaptic potentials, in the absense of any pronounced alterations in membrane resistance. 相似文献
17.
In isolated slices of hypothalamus, suprachiasmatic nucleus (SCN) neurons were recorded intracellularly. Blockade of Ca++ channels increased spike duration, eliminating an early component of the afterhyperpolarization (AHP) that followed evoked spikes. The duration and reversal potential of AHPs were, however, unaffected, suggesting that only an early, fast component of the AHP was Ca(++)-dependent. Unlike other central neurons that exhibit pacemaker activity, therefore, SCN neurons do not display a pronounced, long-lasting Ca(++)-dependent AHP. Extracellular Ba++ and intracellular Cs+ both revealed slow depolarizing potentials evoked either by depolarizing current injection, or by repolarization following large hyperpolarizations. They had different effects on the shape of spikes and the AHPs that followed them, however. Cs+, which blocks almost all K+ channels, dramatically reduced resting potential, greatly increased spike duration (to tens of milliseconds), and blocked AHPs completely. In contrast, Ba++ had little effect on resting potential and produced only a small increase in spike duration, depressing an early Ca(++)-dependent component and a later Ca(++)-independent component of the AHP. The relatively weak pacemaker activity of SCN neurons appears to involve voltage-dependent activation of at least one slowly inactivating inward current, which brings the cells to firing threshold and maintains tonic firing; both Ca(++)-dependent and Ca(++)-independent K+ channels, which repolarize cells after spikes and maintain interspike intervals; and Ca++ channels, which contribute to activation of Ca(++)-activated K+ currents and may also contribute to slow depolarizing potentials. In the absence of powerful synaptic inputs, SCN neurons express a pacemaker activity that is sufficient to maintain an impressively regular firing pattern. Slow, repetitive activation of optic input, however, increases local circuit activity to such an extent that the normal pacemaker potentials are overridden and firing patterns are altered. Since SCN neurons are very small and have large input resistances, they are particularly susceptible to synaptic input. 相似文献
18.
Ca(v)1.2 L-type calcium channels support hippocampal synaptic plasticity, likely by facilitating dendritic Ca2+ influx evoked by action potentials (AP) back-propagated from the soma. Ca2+ influx into hippocampal neurons during somatic APs is sufficient to activate signalling pathways associated with late phase LTP. Thus, mechanisms controlling AP firing of hippocampal neurons are of major functional relevance. We examined the excitability of CA1 pyramidal cells using somatic current-clamp recordings in brain slices from control type mice and mice with the Ca(v)1.2 gene inactivated in principal hippocampal neurons. Lack of the Ca(v)1.2 protein did not affect either affect basic characteristics, such as resting membrane potential and input resistance, or parameters of single action potentials (AP) induced by 5 ms depolarising current pulses. However, CA1 hippocampal neurons from control and mutant mice differed in their patterns of AP firing during 500 ms depolarising current pulses: threshold voltage for repetitive firing was shifted significantly by about 5 mV to more depolarised potentials in the mutant mice (p<0.01), and the latency until firing of the first AP was prolonged (73.2+/-6.6 ms versus 48.1+/- 7.8 ms in control; p<0.05). CA1 pyramidal cells from the mutant mice also showed a lowered initial spiking frequency within an AP train. In control cells, isradipine had matching effects, while BayK 8644 facilitated spiking. Our data demonstrate that Ca(v)1.2 channels are involved in regulating the intrinsic excitability of CA1 pyramidal neurons. This cellular mechanism may contribute to the known function of Ca(v)1.2 channels in supporting synaptic plasticity and memory. 相似文献
19.
Spontaneous and evoked activity of caudate nucleus neurons was recorded extracellularly in acute experiments on cats. Different forms of potentials were found by analysis of the results. The potentials recorded belong to three types: ordinary action potentials; prepotentials or incomplete spikes differing from ordinary action potentials in their lower amplitude and slower decline, and complex discharges in which a spike of somewhat reduced amplitude is followed by a slow positive-negative wave. In the spontaneous activity prepotentials were observed both in complete action potentials and in isolation. The frequency of the complex discharges was 0.5–1 per second. The slow wave of these discharges blocked prepotential and action potential formation. The origin of these forms of potentials in neurons of the caudate nucleus is discussed and they are compared with analogous forms of potentials described for the Purkinje cells of the cerebellum.A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukranian SSR, Kiev. Translated from Neirofiziologiya, Vol. 9, No. 2, pp. 149–156, March–April, 1977. 相似文献
20.
Three neurons capable of generating coordinated bursting activity or synchronized slow-wave fluctuations in membrane potential (MP) were identified in the left parietal ganglion ofHelix pomatia. The function of these units contributes to regulating rhythmic opening and closing movements in the pneumostome. Both bursting activity and slow-wave neuronal MP synchronize with rhythmic movements of the pneumostome. Onset of bursting activity and fluctuations in MP on the one hand or suppression of these effects due to different influences on the cells on the other leads to initiation or extinction of pneumostome movements respectively. These neurons do not exhibit endogenous bursting activity but do produce a fairly high rate of firing activity without bursting pattern and without slow-waves in MP in isolation. Bursting activity occurs in these neurons in the intact central nervous system (CNS) as a result of gigantic synchronized IPSP in some cases and due to the aforementioned slow waves in MP and in others. No direct chemically- or electrically-operated synaptic connections exist between the three cells. Serotonin triggers both waves in MP and bursting activity in all three neurons in the intact CNS and exerts a pronounced hyperpolarizing action on each of these factors in isolation.N. K. Kol'tsov Institute of Developmental Biology, Academy of Sciences of the USSR. Moscow. Balaton Limnological Research Institute of the Hungarian Academy of Sciences, Tihany. Translated from Neirofiziologiya, Vol. 20, No. 4, pp. 509–517, July–August, 1988. 相似文献