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1.
Woo-Jin Kim Young-Ok Kim Bo-Hye Nam Hee Jeong Kong Eun-Mi Park Jung-Ha Kang Jeong-Ho Lee Kyung-Kil Kim 《Conservation Genetics》2009,10(4):1105-1111
Expressed sequence tags (ESTs) can be used to identify microsatellite markers. We developed 81 polymorphic microsatellite
markers from 4,940 ESTs of the olive flounder, Paralichthys olivaceus. Out of 100 EST-derived microsatellites for which PCR primers were designed, 81 loci were polymorphic in 30 individuals from
a single natural population with 2–28 (mean 10.6) alleles per locus. The observed and expected heterozygosities of these loci
were 0.033–1.000 and 0.033–0.965, respectively. Segregation analysis within a mapping family revealed non-amplifying null
alleles at five loci. These new EST-derived microsatellite markers should be useful for population genetic analyses, pedigree
tracing and constructing a linkage map for olive flounder. 相似文献
2.
We report the development of 15 new microsatellite markers for Emys orbicularis and Emys trinacris. A survey of 20 individuals showed that all loci are highly polymorphic with 3–14 alleles per locus. Additionally, 22 Glyptemys muhlenbergii primers were checked for cross-species amplification, with 14 being amplified successfully and polymorphic (2–14 alleles).
A set of eight markers was selected and combined into two multiplex PCRs. Levels of genetic diversity were assessed in 648
individuals covering the complete distribution area. The number of alleles ranged from 13 to 24 and observed heterozygosity
varied between 0.515 and 0.852. 相似文献
3.
A total of 12 polymorphic microsatellite loci were developed and characterized for a Chinese medicinal plant, Epimedium brevicornum (Berberidaceae). A genomic DNA enrichment protocol was used to isolate microsatellite loci and polymorphism was explored
using 38 individuals from one natural population. The observed number of alleles ranged from 2–14. The ranges of observed
and expected heterozygosity were 0.00–0.83 and 0.15–0.88, respectively. In addition, successful cross-species amplification
of this set of microsatellite markers in other four medicinal Epimedium species suggested that they would provide a useful tool for the genetic and conservation studies of Epimedium species. 相似文献
4.
The blue mussel Mytilus trossulus occurs in the Pacific and in the North Atlantic. We developed and characterized six microsatellite loci for Baltic M. trossulus. Seventeen microsatellite loci were screened, of which six were polymorphic. The number of alleles among 50 individuals ranged
from 3 to 13 and the observed and expected heterozygosity were 0.09–0.46 and 0.34–0.86, respectively. The loci were also tested
for cross amplification in M. edulis, in which four of the six microsatellite loci were successfully amplified. 相似文献
5.
We report on the isolation and characterization of 11 microsatellite loci in the Sichuan snub-nosed monkey (Rhinopithecus roxellana) from AC-enriched genomic libraries. 20 microsatellite loci were screened from the libraries, of which 11 were polymorphic.
The number of observed alleles among 32 samples of snub-nosed monkey ranged from three to nine. The observed and expected
heterozygosity were 0.071–0.815 and 0.201–0.819, respectively. The high variability revealed in this study should be useful
tools for further study on social structure and population genetics of snub-nosed monkey in the future. 相似文献
6.
Marta Dubreuil Federico Sebastiani Maria Mayol Santiago C. González-Martínez Miquel Riba Giovanni G. Vendramin 《Conservation Genetics》2008,9(6):1665-1668
Seven polymorphic nuclear microsatellite markers for Taxus baccata L. (English yew) were developed using an enriched-library method. An additional polymorphic SSR was obtained by testing eight
primer pairs from the congeneric species Taxus sumatrana. Mendelian inheritance for the seven Taxus baccata SSRs was proved by genotyping 17 individuals and 124 megagametophytes (conifer seed haploid tissue). A total of 96 individuals
from 5 different populations (10–26 samples per population) were used to estimate genetic diversity parameters. High levels
of genetic diversity, with values ranging from 0.533 to 0.929 (6–28 alleles per SSR) were found. No linkage disequilibrium
between pairs of loci was detected. All loci but one showed significant departures from Hardy–Weinberg equilibrium. Excess
of homozygosity was probably due to high inbreeding in English yew populations, an outcome of low effective population size
and/or fragmented distribution. Highly polymorphic SSRs will be used to conduct population genetic studies at different geographical
scales and to monitor gene flow. 相似文献
7.
Achnatherum sibiricum is a threaten and toxic perennial bunchgrass mainly in the north of China. We developed 11 polymorphic microsatellite loci
from A. sibiricum by combining biotin capture method. After validating and scoring, these loci were polymorphic in a test population with the
range of alleles from four to 13 per locus. The observed and expected heterozygosities ranged from 0.1649 to 0.5624 and from
0.3071 to 0.8826, respectively. All 11 microsatellite markers were in Hardy–Weinberg equilibrium. These polymorphic microsatellites
will be useful for genetic diversity analysis and population structure construction for A. sibiricum. 相似文献
8.
In order to study hybridisation, taxonomic boundaries and phylogeography in the genus Pseudopanax (Araliaceae), we developed seven novel microsatellite loci from enriched genomic libraries of P. lessonii and P. crassifolius. These loci were characterised in 16 individuals from a single population of P. crassifolius, and displayed 2–11 alleles per locus. Observed heterozygosities ranged from 0.063 to 0.688. Most loci were polymorphic in
the closely related Pseudopanax species, and several loci amplified widely across the Araliaceae. 相似文献
9.
Eleven dinucleotide and five tetranucleotide polymorphic microsatellite loci were presented for the Indo-Pacific humpback
dolphin Sousa chinensis, a species categorized as ‘data deficiency’ in the IUCN Red List. These markers were developed to allow future population
studies, such as characterization of population structure and genetic diversity that are important for the species’ conservation.
The number of alleles ranged from 2 to 9 with observed heterozygosity ranging from 0.167 to 0.917, and expected heterozygosity
ranging from 0.159 to 0.913. Test of these loci in five additional cetacean species found that 10–13 loci have successful
cross-amplifications. 相似文献
10.
Shichao Xing Changwei Shao Xiaolin Liao Yongsheng Tian Songlin Chen 《Conservation Genetics》2009,10(3):789-791
In the present study, we reported 13 polymorphic microsatellite loci isolated from a dinucleotide-enriched genomic library
of Nibea albiflora. The number of alleles, observed and expected heterozygosity per locus in 44 individuals ranged from 2 to 13, from 0.0909
to 0.9773 and from 0.0886 to 0.9073, respectively. Three loci significantly deviated from Hardy–Weinberg equilibrium after
Bonferroni correction. Analysis of linkage disequilibrium showed non-significant among the two pairs of loci. As a result,
13 microsatellite loci probably located on different chromosome pairs and these polymorphic microsatellite loci should provide
sufficient level of genetic diversity to investigate the fine-scale population structure and evaluate the breeding strategy
in Nibea albiflora.
Shichao Xing, Changwei Shao contributed equally to this work. 相似文献
11.
Xiaolin Liao Dan Wang Xiaomu Yu Weitao Li Lei Cheng Jianwei Wang Jingou Tong 《Conservation Genetics》2007,8(4):1003-1007
Rare minnow (Gobiocypris rarus) is an endangered small fish endemic to upper reach of the Yangtze River. From a (GT)n enriched genomic library, 32 microsatellites were isolated and characterized. Nineteen of these loci were polymorphic in
a test population with alleles ranging from 2–7, and observed and expected heterozygosities from zero to 0.8438, and 0.2679
to 0.8264, respectively. In the cross-species amplifications, 13 out of 19 polymorphic loci were found to be also polymorphic
in at least one of the 7 closely related species of the subfamily Gobioninae. These polymorphic microsatellite loci should
provide sufficient level of genetic diversity to evaluate the fine-scale population structure in rare minnow and its closely
related species for the conservation purpose. 相似文献
12.
We report on the isolation and characterization of eight microsatellite markers in the Hainan Eld’s deer (Cervus eldi hainanus) from genomic DNA-enriched libraries. Thirty-three microsatellites were screened from the libraries, and 8 of the screened
microsatellites were polymorphic. The number of observed alleles for each locus in 47 individuals ranged from 2 to 9, and
the expected and observed heterozygosity was 0.141–0.792 and 0.128–0.957, respectively. Three loci (CEH-2, CEH-6 and CEH-8)
of eight deviated from Hardy-Weinberg expectation and no significant linkage association was found among all these loci. These
microsatellite markers provide useful tool for population genetic studies of the Eld’s deer. 相似文献
13.
Shi-Chao Xing Gen-Bo Xu Xiao-Lin Liao Guan-Pin Yang Song-Lin Chen 《Conservation Genetics》2009,10(4):1167-1169
In the study, 34 microsatellite loci were isolated from a dinucleotide-enriched genomic library of Japanese Spanish mackerel
(Scomberomorus niphonius). And 12 microsatellite loci were found to be polymorphic between 3 and 8 alleles. The number of observed and expected heterozygosity
per locus in 23 individuals ranged from 0.6087 to 1.0000 and 0.8908 to 0.9773, respectively. Two loci significantly deviated
from Hardy–Weinberg equilibrium after Bonferroni correction analysis and there was no significant linkage disequilibrium found
between pairs of loci. As a result, 12 microsatellite loci probably located on different chromosome pairs and these polymorphic
microsatellite loci should provide sufficient level of genetic diversity to investigate the fine-scale population structure
and evaluate the breeding strategy in S. niphonius.
The authors Shi-Chao Xing and Gen-Bo Xu contributed equally. 相似文献
14.
Kate Meares Deborah A. Dawson Gavin J. Horsburgh Mike R. Perrin Terry Burke Tiawanna D. Taylor 《Conservation Genetics》2008,9(5):1363-1367
Forty-two unique microsatellite loci were isolated from an unenriched and a tetranucleotide-enriched blue crane (Grus paradisea) genomic library. Fourteen polymorphic loci were characterised in 20 unrelated wild blue crane individuals from the Karoo
region, South Africa, and displayed 4–27 alleles with observed heterozygosities ranging between 0.50 and 0.95. All 14 loci
were also polymorphic in the grey-crowned crane (Balearica regulorum) and the wattled crane (Grus carunculatus). These markers are aimed at assisting the identification of illegal trade in the blue crane but have wider population and
conservation applications for most, if not all, crane species. 相似文献
15.
We report on the isolation and characterization of nine microsatellite markers in the takin (Budorcas taxicolor) from genomic DNA-enriched libraries. Twenty-eight microsatellites were screened from the libraries, and nine of the screened
microsatellites were polymorphic. The number of observed alleles for each locus in 28 individuals ranged from two to seven,
and the expected and observed heterozygosity was 0.105–0.758 and 0.071–0.821, respectively. Four loci (TK01, TK02, TK04 and
TK08) of nine deviated from Hardy-Weinberg expectation and no significant linkage association was found among all these loci.
These microsatellite markers provide useful tool for population genetic studies of the takin. 相似文献
16.
Katherine P. Munkres Line K. Bay Dean R. Jerry Mark I. McCormick Lynne Van Herwerden 《Conservation Genetics》2007,8(4):987-990
Five new polymorphic microsatellite loci were developed for the coral reef damselfish Pomacentrus amboinensis. Twenty-four individuals from two Great Barrier Reef populations were genotyped at the five loci, with numbers of alleles
per locus ranging from 6–23 and observed heterozygosity between 0.42–0.92. In addition, the cross-species testing of six primers
developed for Stegastes partitus revealed one primer (SpGATA40) that was also polymorphic for P. amboinensis. Due to high levels of polymorphism (≥14 alleles) in at least four of the six loci and a high proportion of tetranucleotide
repeats, these microsatellite markers should be useful for parentage assignment as well as other investigations of individual
relatedness. 相似文献
17.
Madjid Delghandi Jørgen Stenvik Frank Nilsen Mette Serine Wesmajervi Kjersti Turid Fjalestad Børge Damsgård 《Conservation Genetics》2008,9(3):747-749
Nine polymorphic microsatellite markers were identified by screening of 2464 ESTs derived from a cDNA library of Atlantic
cod (Gadus morhua L.). About 35 novel microsatellite loci were selected and characterised in 96 individual cod. Nine markers were successfully
amplified with number of alleles from 3 to 18 per locus and the average heterozygosity was 0.57 in the panel examined (range
0.29–0.86). All loci followed the Hardy–Weinberg expectation and no significant linkage disequilibrium was found in a test
including all pairwise combinations. The gene identity was determined at four of the loci, confirming the associated microsatellites
as Type I markers. 相似文献
18.
Hai-Long Wu Xiao-Wei Ni Lin-Yuan Zhang Jin-Shi Xia Zhen-Yu Zhong Guo-Ping Zhu Qiu-Hong Wan 《Conservation Genetics》2008,9(6):1679-1682
Père David’s deer is a severely bottlenecked species but without showing inbreeding depression, making it essential to develop
molecular markers to explore her genetic mechanism of population recovery. In this study, we isolated 18 novel polymorphic
microsatellite loci from a dinucleotide-enriched library. This suit of markers presented 2–3 alleles for each locus and their
observed and expected heterozygosities were 0.057–0.610 and 0.056–0.598, respectively. These new microsatellite loci had an
average of 2.12 alleles and thus contributed to relatively low exclusion probabilities of parentage and paternity testing
(0.768 and 0.921). However, when these loci were examined in combination with previous microsatellite markers, overall probabilities
of parentage and paternity exclusion went up to 0.905 and 0.990, respectively, showing that these 26 microsatellite loci should
be adopted together in future genetic analyses for this highly inbred species. 相似文献
19.
María Calero-Riestra Fernando Alda Jose Antonio Dávila Jesús T. García 《European Journal of Wildlife Research》2012,58(1):379-383
New microsatellite loci for Tawny Pipit Anthus campestris were isolated from a genomic library. We were able to unambiguously score six loci: two were dinucleotide, one trinucleotide,
two tetranucleotide and one pentanucleotide that turned out to be sex-linked. Four out of six loci were polymorphic with 7–23
alleles in our population and an observed heterozygosity ranging between 0.286 and 0.936. Cross-utility of these markers was
tested in other 17 steppe-bird species of six families. In addition, 16 microsatellite loci developed for other species were
tested for cross-species amplification in A. campestris. Eight microsatellite markers were successfully amplified; seven of them were polymorphic with 2–43 alleles and an observed
heterozygosity of 0.040–0.863. Overall, 14 functional locus markers have been characterized for A. campestris that could be useful for future studies of paternity, genetic variability and population structure. 相似文献
20.
Yong-Sheng Tian Gui-Dong Miao Chang-Wei Shao Xiao-Lin Liao Song-Lin Chen 《Conservation Genetics》2009,10(4):1041-1043
Stone flounder (Kareius bicoloratus) is a rare fish species in China. Here, we reported 11 polymorphic microsatellite loci isolated from a repeat-enriched genomic
library of stone flounder. The number of alleles, observed and expected heterozygosity per locus in 32 individuals ranged
from 3 to 6, from 0.1613 to 0.8667 and from 0.1549 to 0.7932, respectively. Two loci significantly deviated from Hardy–Weinberg
equilibrium after Bonferroni correction and no significant linkage disequilibrium between pairs of loci was found. Cross-species
amplification of these microsatellite loci in additional three fish species was performed. These polymorphic microsatellite
loci would be useful for investigating genetic population structure and construction of genetic linkage map in Kareius bicoloratus.
Yong-Sheng Tian and Gui-Dong Miao are contributed equally. 相似文献