现代生物医学进展编辑委员会

名誉主编:(HoNORARY EDIT()R一IN一CHIEF)刘颂豪(LIU song一hao)傅世英(r[}shi一ying)工酒谦(WANc Nai一qian)主编:(El)ITOR一IN一CHIEF)刘文成(LIU wen一。heng)周晋(zHOU Jin)副主编:(ASSOCIATE EDIT()RS一IN一CHIEF)唐一源(TAN(;Yi一yuan)王国栋(wANG Gu〔卜‘long)黄培林(H     

现代生物医学进展编辑委员会

名誉主编:《HONORARY EDITOR一IN一CHIEF)刘颂豪(Llu song一hao)阮长耿(RuAN ehang一geng)曾溢滔(zENG Yi一tao)傅世英(FU shi一ying)王酒谦(wANe Nai一qian)主编:(EDIToR一IN一eHIEF)周晋(zHou Jin)李钮(Ll四)副主编:(ASSoCIATE EDIToRS一州一eHxEF)唐一源(TANo Yi一”an)曾凡一(zENo Fan一扒)邓红文(oENo Hong一wen)王国栋(wANG ouo一dong)黄培林(HLJANo Pei一lin)代文杰(oAI wen一ie)张清媛(zHANo Qin一四an)刘同奎(L川Tong一kui)编委:《EDITORS) (以姓氏汉语拼音为序)卞卡(B认NKa)米蔡维君(e…     

水稻多元有害生物为害特征及产量损失量化

收集了云南粳稻主产区沾益、寻甸两县106块稻田水稻有害生物为害和产量等信息,并用两种方法对其分析。第一种方法应用聚类分析和对应分析描述水稻有害生物为害类型和产量水平之间的关系,第二种方法应用主成分分析和多元逐步回归估计各为害的产量损失。聚类分析确定了7种有害生物为害类型,其中IN1、IN2和IN3为害水平较低,而IN5、IN6和IN7为害水平较高。有害生物为害类型和水稻产量水平之间的对应分析,在前两个轴构成的因子空间内绘出了各为害类型和产量水平的位置,并给出了一条与为害类型紧密联系的产量水平增加路线。该分析暗示与位于该因子平面右边的有害生物为害类型(IN1、IN2和IN3)相比,位于左边的为害类型(IN5、IN6和IN7)将引起水稻更大的产量损失。主成分多元回归分析评估了水稻各种病、虫、草害所造成的减产量及其相对重要性。分析结果表明,高于水稻冠层杂草、蛀茎害虫(白穗)、稻纵卷叶螟、白叶枯病、粘虫、叶瘟病和稻飞虱是该稻作区对水稻产量影响较大的为害因子。     

T66I突变提高HIV-1整合酶大肠杆菌表达可溶性研究

在研究HIV-1整合酶(IN)抗药性突变T66I时,发现这一突变同时可以提高整合酶的溶解性。原核表达了IN1?288/T66I和野生型(WT),取菌体破碎后的上清,SDS-PAGE和his标签蛋白质染色进行分析,结果表明IN1?288/T66I可溶性约是WT的2.4倍。600ml培养基中诱导表达IN1?288/T66I/BL21,亲和层析纯化共收获蛋白质4.72mg。用改进的ELISA方法测定IN1?288/T66I和IN1-288/F185K/C280S链转移催化活性,结果显示两种蛋白质活性基本相当。提供了有别于F185K/C280S突变的另外一种整合酶可溶性表达的途径,IN1?288/T66I重组蛋白还可以应用到整合酶抑制剂筛选中,以获取避开T66I抗药性突变的抑制剂。     

SCIENCE IN CHINA(SCIENTIA SINICA)

~~SCIENCE IN CHINA(SCIENTIA SINICA)     

《植物生理与分子生物学学报》编辑委员会

编委会顾问(Advisers to the Edito五al Board):(以姓氏拼音为序) 洪孟民(HONG Meng一Min)匡廷石(KUANG Ting一Yun) 沈允钢(SHEN Yun一Gang)施教耐(SHI Jiao一Nai) 袁隆平(YuAN Long一Ping)主编(Editor一in一Chieo:许大全(XU Da一Quan)副主编(Assoeiate Editors一in一e址eo:(以姓氏拼音为序) 陈晓亚(CHENXiao一Ya)瞿伟氰QUwei一Jing) 许煌泉(xu Yu一Quan)主编顾问(Adviser to the Editor一in一ChieO:王天铎(WANG Tian一Duo)编委(Members of the Board):(以姓氏拼音为序,*为常务编委) 曹仪植(CAo Yi一z址)种康’(…     

新版图书推介

正《系统解剖学(第4版)》名誉主编:钟世镇徐达传主编:廖华书号:978-7-04-049567-6出版时间:2018年5月定价:79.00元《妇产科学(双色版)》主编:赵霞马丁书号:978-7-04-048813-5出版时间:2018年2月定价:59.80元《药理学(第3版)》主编:颜光美书号:978-7-04-047167-0出版时间:2018年02月定价:68.00元     

Retroviral integrase protein and intasome nucleoprotein complex structures

Retroviral replication proceeds through the integration of a DNA copy of the viral RNA genome into the host cellular genome, a process that is mediated by the viral integrase(IN) protein. IN catalyzes two distinct chemical reactions: 3'-processing, whereby the viral DNA is recessed by a di- or trinucleotide at its 3'-ends, and strand transfer, in which the processed viral DNA ends are inserted into host chromosomal DNA. Although IN has been studied as a recombinant protein since the 1980 s, detailed structural understanding of its catalytic functions awaited high resolution structures of functional IN-DNA complexes or intasomes, initially obtained in 2010 for the spumavirus prototype foamy virus(PFV). Since then, two additional retroviral intasome structures, from the α-retrovirus Rous sarcoma virus(RSV) and β-retrovirus mouse mammary tumor virus(MMTV), have emerged. Here, we briefly review the history of IN structural biology prior to the intasome era, and then compare the intasome structures of PFV, MMTV and RSV in detail. Whereas the PFV intasome is characterized by a tetrameric assembly of IN around the viral DNA ends, the newer structures harbor octameric IN assemblies. Although the higher order architectures of MMTV and RSV intasomes differ from that of the PFV intasome, they possess remarkably similar intasomal core structures. Thus, retroviral integration machineries have adapted evolutionarily to utilize disparate IN elements to construct convergent intasome core structures for catalytic function.     

The Second Editorial Board of Chinese Journal of Biotechnology

ADVISORJIAORui Shen (J.S .Chao)　　Professor(InstituteofPlantPhysiologyandEcology,ShanghaiInstitutesforBiologicalSciences,ChineseAcademyofSciences,Shanghai2 0 0 0 32 ,China)EDITOR IN CHIEFYANGSheng Li　　Academician(ShanghaiResearchCenterforBiotechnology ,ChineseAcademyofSciences,Shanghai 2 0 0 2 33,China)VICE EDITOR IN CHIEF (alphabetically)FANGRong Xiang　　Academician(InstituteofMicrobiology ,ChineseAcademyofSciences,Beijing 10 0 0 80 ,China)HEFu Chu　　A…     

The Eighth Editorial Board of Acta Microbiologica Sinica

EDITOR IN CHIEFLIJi Lun　Academician(CollegeofBiology,ChineseAgriculturalUniversity,Beijing 10 0 0 94 ,China)VICE EDITOR IN CHIEFTANHua Rong　Professor(InstituteofMicrobiology ,ChineseAcademyofSciences,Beijing 10 0 0 80 ,China)LUDe Ru　Professor(InstituteofGenetics,SecondMilitaryMedicalUniversity ,Shanghai 2 0 0 4 33,China)WANGAo Quan　Professor(InstituteofMicrobiology ,ChineseAcademyofSciences,Beijing 10 0 0 80 ,China)QUYin Bo　Professor(SchoolofLifeScience ,…