共查询到20条相似文献,搜索用时 31 毫秒
1.
In the present work, the effect of LiCl on phosphoenolpyruvate carboxylase kinase (PEPCase-k), C4 phosphoenolpyruvate carboxylase (PEPCase: EC 4.1.1.31) and its phosphorylation process has been investigated in illuminated
leaf disks and leaves of the C4 plant Sorghum vulgare. Although this salt induced severe damages to older leaves, it did not significantly alter the physiological parameters (photosynthesis,
transpiration rate, intercellular CO2 concentration) of young leaves. An immunological approach was used to demonstrate that the PEPCase-k protein accumulated
rapidly in illuminated leaf tissues, consistent with the increase in its catalytic activity. In vivo, LiCl was shown to strongly
enhance the light effect on PEPCase-k protein content, this process being dependent on protein synthesis. In marked contrast,
the salt was found to inhibit the PEPCase-k activity in reconstituted assays and to decrease the C4 PEPCase content and phosphorylation state in LiCl treated plants. Short-term (15 min) LiCl treatment increased IP3 levels, PPCK gene expression, and PEPCase-k accumulation. Extending the treatment (1 h) markedly decreased IP3 and PPCK gene expression, while PEPCase-k activity was kept high. The cytosolic protein synthesis inhibitor cycloheximide (CHX), which
blocked the light-dependent up-regulation of the kinase in control plants, was found not to be active on this process in preilluminated,
LiCl-treated leaves. This suggested that the salt causes the kinase turnover to be altered, presumably by decreasing degradation
of the corresponding polypeptide. Taken together, these results establish PEPCase-k and PEPCase phosphorylation as lithium
targets in higher plants and that this salt can provide a means to investigate further the organization and functioning of
the cascade controlling the activity of both enzymes. 相似文献
2.
T. O. Yastreb Yu. E. Kolupaev A. A. Lugovaya A. P. Dmitriev 《Applied Biochemistry and Microbiology》2017,53(6):719-724
The effect of hydrogen peroxide treatment on the salt tolerance of wild-type Arabidopsis thaliana L. plants (Col-0) and plants transformed with the bacterial salicylate hydroxylase gene (NahG) was studied. The base tolerance to salt stress caused by 200 mM of NaCl in solution culture was higher in plants with the NahG genotype in comparison with the wild-type plants. Growth inhibition was observed for wild-type plants under the action of exogenous hydrogen peroxide, which was not observed for the NahG transformants; salt tolerance increased in the both types of plants after treatment, which was assessed based on the growth indicators and the ability to preserve the chlorophyll pool following NaCl treatment. The content of endogenous Н2О2 in the leaves of wild-type plants increased significantly following exogenous hydrogen peroxide treatment and salt stress, while it practically did not change in the leaves of the NahG genotype. The SOD activity increased in both genotypes after treatment with exogenous hydrogen peroxide, and remained at an elevated level after salt stress in comparison with the nontreated plants. Furthermore, the catalase activity increased in leaves of the salicylate-deficient genotype but not in the Col-0 genotype. The guaiacol peroxidase activity increased in plants of both genotypes under the action of hydrogen peroxide and salt stress, with the NahG plants demonstrating a higher degree of increase. The Н2О2 treatment facilitated the increase of the proline content in leaves of the plants of both genotypes under conditions of salt stress. It was concluded that there were hydrogen peroxide signal transduction pathways in Arabidopsis plants that were salicylic acid independent and that the antioxidant system functioned more effectively in salicylate-deficient Arabidopsis plants. 相似文献
3.
Aquatic-living colonial filaments of the terrestrial cyanobacterium Nostoc flagelliforme, developed from single cells in laboratory under aquatic conditions, were cultured at different salt concentrations (0–400 mM), and their photosynthetic responses were investigated to see their physiological tolerance. Light-saturated photosynthesis, photosynthetic efficiency and dark respiration showed the highest values in treatments at 20 mM NaCl for 24 or 48 h incubation. Changes in salt level exerted little influence on light saturation point and light compensation point. Patterns of photosynthetic performance as a function of salt were the same after 48 h as those after 24 h treatment, with the largest values at 20 mM NaCl, though photochemical efficiency increased with increased NaCl concentrations in the colonies treated for 48 h. From an applied point of view, the laboratory-generated aquatic living colonies are able to tolerate salt stress when transferred from aquatic to terrestrial environments. 相似文献
4.
W. Liu Y. Zhang X. Yuan Y. Xuan Y. Gao Y. Yan 《Russian Journal of Plant Physiology》2016,63(1):132-142
In the present study, the physiological responses of Nitraria tangutorum Bobr. seedlings to NaCl stress and the regulatory function of exogenous application of salicylic acid (SA) were investigated. NaCl in low concentration (100 mM) increased while in higher concentrations (200–400 mM) decreased the individual plant dry weights (wt) of seedlings. Decreased relative water content (RWC) and chlorophyll content were observed in the leaves of seedlings subjected to salinity stress (100–400 mM NaCl). Furthermore, NaCl stress significantly increased electrolyte leakage and malondialdehyde (MDA) content. The levels of osmotic adjustment solutes including proline, soluble sugars, and soluble protein were enhanced under NaCl treatments as compared to the control. In contrast, exogenous application of SA (0.5–1.5 mM) to the roots of seedlings showed notable amelioration effects on the inhibition of individual plant dry wt, RWC, and chlorophyll content. The increases in electrolyte leakage and MDA content in the leaves of NaCl-treated seedlings were markedly inhibited by SA application. The SA application further increased the contents of proline, soluble sugars, and soluble protein. The activities of antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) were up-regulated by NaCl stress and the activities of SOD, POD, and CAT were further enhanced by SA treatments. Application of SA in low concentration (0.5 mM) enhanced while in higher concentrations (1.0 and 1.5 mM) inhibited APX activities in leaves of NaCl-treated seedlings. These results indicate that SA effectively alleviated the adverse effects of NaCl stress on N. tangutorum. 相似文献
5.
Cristina Echevarría Sofía Garcia-Mauriño Rosario Alvarez Andrés Soler Jean Vidal 《Planta》2001,214(2):283-287
In C4 plants, the photosynthetic enzyme phosphoenolpyruvate carboxylase (PEPCase; EC 4.1.1.31) is subjected to a phosphorylation process via the light-dependent up-regulation of a Ca2+-independent PEPCase-kinase. The present work aimed to study the effect of salt stress on PEPCase phosphorylation in Sorghum vulgare Pers. leaves. The growth of salt-treated plants was reduced compared with that of the control plants. PEPCase activity modestly increased (around 20-40%) whereas PEPCase phosphorylation was markedly enhanced, on a protein basis, in extracts from illuminated leaves. The enhanced protein kinase activity was found to display a low molecular mass in the range 32-35 kDa, to be independent of Ca2+ and to be up-regulated by light. Furthermore, up-regulation was blocked in vivo by the cytosolic protein synthesis inhibitor cycloheximide. Collectively, these data demonstrated that salinity stress altered the Ca2+-independent PEPCase-kinase, presumably by increasing the mesophyll content of the enzyme. Potassium chloride, but not abscisic acid, mimicked the effect of NaCl on PEPCase-kinase activity. 相似文献
6.
G. Urbinati P. Nota A. Frattarelli P. Di Cori S. Lucioli C. Forni E. Caboni 《Acta Physiologiae Plantarum》2018,40(8):151
The physiological and antioxidant response to salinity was studied in pomegranate (Punica granatum L.) by exposing in vitro growing shoots of the Italian variety Profeta Partanna to 125 or 250 mM NaCl for 10 and 20 days. 250 mM NaCl significantly reduced shoot length, leaf area and water content of the shoots, regardless the length of the salt treatment,with respect to the control and to the 125 mM NaCl treatment. After 20 days the shoots treated with 250 mM NaCl also showed a significant reduction in relative growth rate (RGR) together with marked necroses and abscission of the oldest leaves. Salt treatments significantly decreased the contents of chlorophylls and carotenoids in both exposure times, depending on NaCl concentration. Proline, total phenolic compounds and ellagic acid did not increase or even decrease with the salt treatments. The levels of lipid peroxidation decreased, ascorbate peroxidase (APX) activity significantly increased in both treatment times and concentrations, while guaiacol peroxidase (G-POD) activity significantly increased in shoots treated with 250 mM NaCl for 20 days suggesting the rapid involvement of APX in controlling the oxidative stress in this species, even at low salt concentrations, and a delayed complementary role of G-POD. 相似文献
7.
8.
HuiPing Mao Yoshikazu Okada Shizuka Michimata Wen Wang Fumiko Iwanaga Norikazu Yamanaka Fukuju Yamamoto 《Landscape and Ecological Engineering》2010,6(1):11-21
Growth, photosynthesis, and Na+, K+, Ca2+, and Mg2+ distributions were examined in two-year-old hydroponically cultured Populus nigra and Populus alba cuttings exposed to salt stress (0, 50, or 100 mM NaCl) for four or six weeks and to nonaeration stress for one or three weeks,
followed by a three-week aeration period in 2/5 Hoagland solution. Salt stress with 100 mM NaCl totally inhibited height increase
in P. nigra cuttings. Combined salinity and nonaeration inhibited height increase to a greater degree than either stress alone in both
species. Simple salt stress did not affect diameter increase in P. alba, whereas combined high salinity (100 mM NaCl) and nonaeration inhibited diameter increase. Growth and biomass accumulation
were more sensitive to salt stress in P. nigra cuttings than in P. alba, although P. alba showed a more rapid decrease in photosynthesis in response to nonaeration stress. Ion distributions in the leaves and roots
differed between species. P. alba was superior to P. nigra in terms of Na+ exclusion capacity, such that most of the absorbed Na+ was confined to the root system, with little reaching the leaves. The distributions of K+, Ca2+, and Mg2+ in the leaves and roots of each species under the two stressors were also analyzed. The lower Na+/K+ ratio in leaves indicated that P. alba was more tolerant to salt stress than P. nigra. 相似文献
9.
Amal Fadl Abdelkader Henrik Aronsson Christer Sundqvist 《Acta Physiologiae Plantarum》2010,32(5):971-978
To examine the effects of salt stress on dark-grown wheat (Triticum aestivum), seedlings of the salt-tolerant cultivar Sids 1 and the susceptible cultivar Giza 168 were grown in darkness for 14 days
in nutrient solution with and without 200 mM of supplementary salt (100 mM of NaCl and 100 mM of KCl). During this time, we
monitored their protochlorophyllide (Pchlide) contents, ratios of photoactive to non-photoactive forms of Pchlide (from 655/633-nm
emission ratios in their 77 K fluorescence emission spectra) and (following flash irradiation) ratios of newly formed chlorophyllide
(Chlide) to non-photoactive Pchlide. In addition, the accumulation of chlorophyll a in leaf sections was monitored during prolonged (24 h) irradiation. The results depended on the developmental state of the
seedlings. However, the salt stress treatment caused marked increases in both Pchlide contents in dark-grown leaves and in
Chlide contents following irradiation of leaf sections of both cultivars. The ratio of phototransformable to non-phototransformable
Pchlide and the abundance of newly formed Chlide were also increased by the salt stress. Further, leaves of salt-stressed
seedlings consistently accumulated more chlorophyll a than leaves of unstressed seedlings when floating on the nutrient solution (with or without supplementary salt) in continuous
white light. The findings are consistent with the hypothesis that increased levels of the long-wavelength form of Pchlide
contribute to protective mechanisms against salt stress. 相似文献
10.
Karim Ben Hamed Antonella Castagna Elkahoui Salem Annamaria Ranieri Chedly Abdelly 《Plant Growth Regulation》2007,53(3):185-194
The present study was carried out to compare the effect of NaCl on growth, cell membrane damage, and antioxidant defences
in the halophyte Crithmum maritimum L. (sea fennel). Physiological and biochemical changes were investigated under control (0 mM NaCl) and saline conditions
(100 and 300 mM NaCl). Biomass and growth of roots were more sensitive to NaCl than leaves. Roots were distinguished from
leaves by increased electrolyte leakage and high malondialdehyde (MDA) concentration. Superoxide dismutase (SOD), catalase
(CAT) and ascorbate peroxidase (APX) activities, ascorbic acid (AA) and glutathione (GSH) concentrations were lower in the
roots than in the leaves of control plants. The different activity patterns of antioxidant enzymes in response to 100 and
300 mM NaCl indicated that leaves and roots reacted differently to salt stress. Leaf CAT, APX and glutathione reductase (GR)
activities were lowest at 300 mM NaCl, but they were unaffected by 100 mM NaCl. Only SOD activity was reduced in the latter
treatment. Root SOD activity was significantly decreased in response to 300 mM NaCl and root APX activity was significantly
higher in plants treated with 100 and 300 mM compared to the controls. The other activities in roots were insensitive to salt.
The concentration of AA decreased in leaves at 100 and 300 mM NaCl, and in roots at 300 mM NaCl, when compared to control
plants. The concentrations of GSH in NaCl-treated leaves and roots were not significantly different from the controls. In
both organs, AA and GSH were predominating in the total pool in ascorbic acid and glutathione, under control or saline conditions. 相似文献
11.
José A. Monreal Cirenia Arias-Baldrich Vanesa Tossi Ana B. Feria Alfredo Rubio-Casal Carlos García-Mata Lorenzo Lamattina Sofía García-Mauriño 《Planta》2013,238(5):859-869
Nitric oxide (NO) is a signaling molecule that mediates many plant responses to biotic and abiotic stresses, including salt stress. Interestingly, salinity increases NO production selectively in mesophyll cells of sorghum leaves, where photosynthetic C4 phosphoenolpyruvate carboxylase (C4 PEPCase) is located. PEPCase is regulated by a phosphoenolpyruvate carboxylase-kinase (PEPCase-k), which levels are greatly enhanced by salinity in sorghum. This work investigated whether NO is involved in this effect. NO donors (SNP, SNAP), the inhibitor of NO synthesis NNA, and the NO scavenger cPTIO were used for long- and short-term treatments. Long-term treatments had multifaceted consequences on both PPCK gene expression and PEPCase-k activity, and they also decreased photosynthetic gas-exchange parameters and plant growth. Nonetheless, it could be observed that SNP increased PEPCase-k activity, resembling salinity effect. Short-term treatments with NO donors, which did not change photosynthetic gas-exchange parameters and PPCK gene expression, increased PEPCase-k activity both in illuminated leaves and in leaves kept at dark. At least in part, these effects were independent on protein synthesis. PEPCase-k activity was not decreased by short-term treatment with cycloheximide in NaCl-treated plants; on the contrary, it was decreased by cPTIO. In summary, NO donors mimicked salt effect on PEPCase-k activity, and scavenging of NO abolished it. Collectively, these results indicate that NO is involved in the complex control of PEPCase-k activity, and it may mediate some of the plant responses to salinity. 相似文献
12.
Nitraria retusa and Atriplex halimus (xero-halophytes) plants were grown in the range 0–800 mM NaCl while Medicago arborea (glycophyte) in 0–300 mM NaCl. Plants were harvested after 120 days of salt-treatment. The present study was designed to
study the effect of salinity on root, stem and leaf anatomy, water relationship, and plant growth in greenhouse conditions.
Salinity induced anatomical changes in the roots, stems and leaves. The cuticle and epidermis of N. retusa and A. halimus stems were unaffected by salinity. However, root anatomical parameters (root cross section area, cortex thickness and stele
to root area ratio), and stem anatomical parameters (stem cross section area and cortex area) were promoted at 100–200 mM
NaCl. Indicating that low to moderate salinity had a stimulating effect on root and stem growth of these xero-halophytic species.
At higher salinities, root and stem structures were altered significantly, and their percentages of reduction were higher
in A. halimus than in N. retusa whereas, in M. arborea, they were strongly altered as salinity rose. NaCl (100–300 mM) reduced leaf water content by 21.2–56.2% and specific leaf
area by 51–88.1%, while increased leaf anatomical parameters in M. arborea (e.g. increased thickness of upper and lower epidermis, palisade and spongy mesophyll, entire lamina, and increased palisade
to spongy mesophyll ratio). Similar results were evidenced in A. halimus leaves with salinity exceeding 100 mM NaCl. Leaves of N. retusa were thinner in salt-stressed plants while epidermis thickness and water content was unaffected by salinity. The size of
xylem vessel was unchanged under salinity in the leaf’s main vein of the three species while we have increased number in M. arborea leaf main vein in the range of 200–300 mM NaCl. A longer distance between leaf vascular bundle, a reduced size and increased
number of xylem vessel especially in stem than in root vascular system was evidenced in M. arborea treated plants and only at (400–800 mM) in the xero-halophytic species. The effects of NaCl toxicity on leaf, stem and root
ultrastructure are discussed in relation to the degree of salt resistance of these three species. Our results suggest that
both N. retusa and A. halimus show high tolerance to salinity while M. arborea was considered as a salt tolerant species. 相似文献
13.
Mónica Calvo-Polanco Melanie D. Jones Janusz J. Zwiazek 《Acta Physiologiae Plantarum》2009,31(3):515-522
In the present study, we investigated the effects of pH treatments on NaCl tolerance in mycorrhizal and non-mycorrhizal American
elm. American elm (Ulmus americana) seedlings were inoculated with Hebeloma crustuliniforme, Laccaria bicolor or with both mycorrhizal fungi and subsequently subjected to different pH solutions (pH 3, 6 and 9) containing 0 mM (control)
and 60 mM NaCl for 4 weeks. Inoculation with the mycorrhizal fungi did not have a large effect on seedling dry weights when
the pH and NaCl treatments were considered independently. However, when the inoculated seedlings were treated with 60 mM NaCl
at pH 3 or 6, shoot to root ratios and root hydraulic conductivity were higher compared with non-inoculated plants, likely
reflecting changes in seedling water flow properties. At pH 6, transpiration rates were about twofold lower in non-inoculated
plants treated with NaCl compared with non-treated controls. For NaCl-treated H. crustuliniforme- and L. bicolor-inoculated plants, the greatest reduction of transpiration rates was at pH 9. Treatment with 60 mM NaCl reduced leaf chlorophyll
concentrations more in non-inoculated compared with inoculated plants, with the greatest, twofold, decrease occurring at pH
6. At pH 3, root Na concentrations were higher in inoculated than non-inoculated seedlings; however, there was no effect of
inoculation on root Na concentrations at pH 6 and 9. Contrary to the roots, the leaves of inoculated plants had lower Na concentrations
at pH 6 and 9, but not at pH 3. The results point to an interaction between ECM fungi and root zone pH for salt tolerance
of American elm. 相似文献
14.
Rym Kaddour Olfa Baatour Hela Mahmoudi Nawel Nasri Maha Zaghdoudi Mokhtar Lachaâl 《Acta Physiologiae Plantarum》2011,33(6):2083-2090
The response to salt treatment and K+ provision of two Arabidopsis thaliana accessions grown for 17 days in the presence of 50 mM NaCl was investigated. Leaf and root dry weight deposition was restricted
by salt, more in Col accession than in NOK2 accession. In both accessions, the growth inhibition induced by salinity was associated
with a decrease in total leaf surface area, which resulted from diminished leaf number, but not from restriction of individual
leaf surface area. Comparing the effects of salt on dry matter production and total leaf surface area revealed large difference
between Col and NOK2 for net assimilation rate (the amount of whole plant biomass produced per unit leaf surface area), which
was augmented by salt and K+ in NOK2 but not in Col. This result, which suggested a better capacity of NOK2 to preserve its photosynthetic machinery against
salt stress, was in agreement with the effect of NaCl on photosynthetic pigments. Indeed, salt significantly reduced chlorophyll
and carotenoid content in Col leaves but had no impact on NOK2 leaf pigment content. Since K+ provision had only marginal effects on these responses to salt stress, leaf mineral unbalance was unlikely. Guaiacol peroxidase
activity was augmented by salt treatment in leaves and roots of both accessions. Salinity decreased the catalase activity
in Col leaves and in roots, and increased this activity in NOK2 organs. In conclusion, when aggressed by salt, NOK2 was able
(1) to produce more leaves than Col, and (2) to efficiently protect its photosynthetic apparatus, perhaps by developing more
efficient antioxidative defense through increased catalase and peroxidase activities. Consequently, the overall photosynthetic
activity was higher and more robust to salt aggression in NOK2 than in Col. 相似文献
15.
Shashank Kumar Mishra Mohammad Haneef Khan Sankalp Misra Vijay Kant Dixit Praveen Khare Suchi Srivastava Puneet Singh Chauhan 《Antonie van Leeuwenhoek》2017,110(2):253-270
Soil bacteria may have properties of plant growth promotion but not be sufficiently beneficial for plants under stress conditions. This challenge has led researchers to extend their searches into extreme environments for potential soil bacteria with multiple plant beneficial traits as well as abiotic stress tolerance abilities. In the current study, an attempt was made to evaluate soil bacteria from an extreme environment, volcano soils, based on plant growth promoting and abiotic stress mitigating characteristics. The screening led to the isolation of eight (NBRISH4, NBRISH6, NBRISH10, NBRISH11, NBRISH13, NBRISH14, NBRISH16 and NBRISH26) bacterial isolates capable of withstanding stresses, namely temperature (up to 45 °C), salt (up to 2 M NaCl) and drought (up to 60% Poly Ethylene Glycol 6000) in vitro. Further, the selected isolates were notable for their in vitro temporal performance with regards to survival (in terms of colony count), phosphate solubilisation, biofilm formation, auxin, alginate and exo-polysaccharide production abilities under abiotic stresses i.e. 40 °C temperature; 500 mM NaCl salt and drought (PEG) conditions. In vivo seed treatments of individual selected bacteria to maize plants resulted into significant enhancement in root and shoot length, root and shoot fresh and dry weight and number of leaves per plant. Overall, the plant growth promoting and abiotic stress tolerance ability was most evident for bacterial isolate NBRISH6 which was identified as an Ochrobactrum sp. using 16S rRNA based phylogenetic analysis. 相似文献
16.
V. M. Passos N. O. Santana F. C. Gama J. G. Oliveira R. A. Azevedo A. P. Vitória 《Biologia Plantarum》2005,49(2):285-288
The effects of treatment with NaCl (3, 100 and 300 mM) for 1, 2, 3 and 7 d on plant growth and ion accumulation were analyzed in 2-week and 8-week-old Annona muricata and A. squamosa plants. Fresh mass and root growth inhibition were directly related to the increase in salinity, particularly for A. squamosa. Two-weeks old seedlings were sensitive to 100 and 300 mM NaCl particularly after 7 d, whereas 8-week-old plants were shown to be more resistant to NaCl even at 300 mM NaCl. Na+ and Cl– mostly accumulated in young leaves. Our results suggest that A. squamosa is more sensitive than A. muricata to salt stress and that older seedlings of both species are more tolerant than younger seedlings. 相似文献
17.
T. O. Yastreb Yu. E. Kolupaev A. A. Lugovaya A. P. Dmitriev 《Cytology and Genetics》2017,51(5):325-330
18.
Q. Fariduddin T. A. Khan M. Yusuf S. T. Aafaqee R. R. A. E. Khalil 《Photosynthetica》2018,56(3):750-762
Salicylic acid (SA) and polyamines (PA) are widely used to overcome various abiotic stresses including salt (NaCl) stress in plants. In the present investigation, co-application efficacies of SA and PA on the salt stress (200 mM NaCl) were evaluated in Lycopersicon esculentum. After transplantation, at 10-d stage, seedlings were exposed to NaCl through soil and then allowed to grow till 30-d stage. At 31-d stage of growth, plants were sprayed with double distilled water (control) or spermidine (1.0 mM) and/or SA (10–5 M). The salt stress significantly reduced the growth, gas-exchange parameters, but increased antioxidant enzymes and proline content in the leaves. Moreover, the loss caused by salt stress was successfully restored by the following treatment of spermidine and SA. 相似文献
19.
Hamid Sobhanian Ferdous Rastgar Jazii Khadija Razavi Vahid Niknam Setsuko Komatsu 《Russian Journal of Plant Physiology》2010,57(6):784-791
To investigate the salt tolerance mechanisms, Aeluropus lagopoides as a halophytic plant was used. Plants were treated with 0, 150, 450, 600, and 750 mM NaCl and harvested at 0, 4, 8, and
10 days after treatment and 1 day and 1 week after recovery. Optimal growth, measured as fresh and dry weights, occurred at
150 mM NaCl, but it was suppressed by 450, 600, and 750 mM NaCl. Recovery significantly increased fresh and dry weights only
in 750 mM NaCl-treated plants. Water content was decreased after NaCl treatment and increased after recovery. Na+ and proline contents and activity of superoxide dismutase (SOD) were increased after NaCl treatment and decreased after recovery
in all treated plants. In contrast, K+ content and ascorbate peroxidase activity decreased after NaCl treatment and increased after recovery in all treated plants.
Catalase (CAT) was activated only in 750 mM NaCl-treated plants. Total content of soluble protein was slightly changed after
NaCl treatment. It was concluded that proline accumulation for osmotic adjustment, SOD activation for O2·− scavenging, and CAT activation at the higher level of salt stress to detoxify produced H2O2 were main A. lagopoides strategies under salt stress. A. lagopoides salt tolerance was not based on the restriction of Na+ uptake. 相似文献
20.
The effects of NaCl stress on growth, water status, contents of protein, proline, malondialdehyde (MDA), various sugars and
photosynthetic pigments were investigated in seedlings of Salicornia persica and S. europaea grown in vitro. Seeds were germinated under NaCl (0, 100, 200, 300, 400, 500 and 600 mM) on Murashige and Skoog medium for 45 d. The shoot
growth of both species increased under low NaCl concentration (100 mM) and then decreased with increasing NaCl concentrations.
In contrast to S. persica, root length in S. europaea reduced steadily with an increase in salinity. Proline content in S. persica was higher than in S. europaea at most NaCl concentrations. Proline, reducing saccharide, oligosaccharide and soluble saccharide contents increased under
salinity in both species. In contrast, contents of proteins and polysaccharides reduced in both species under salt stress.
MDA content remained close to control at moderate NaCl concentrations (100 and 200 mM) and increased at higher salinities.
MDA content in S. europaea was significantly higher than S. persica at higher salinities. Salt treatments decreased K+ and P contents in seedlings of both species. Significant reduction in contents of chlorophylls and carotenoids due to NaCl
stress was also observed in seedlings of both species. Some differences appeared between S. persica and S. europaea concerning proteins profile. On the basis of the data obtained, S. persica is more salt-tolerant than S. europaea. 相似文献