Interspecific microsatellite markers for the study of pinniped populations

Microsatellites have rapidly become the marker of choice for a wide variety of population genetic studies. Here we describe 20 pinniped microsatellite markers which have been tested across 18 pinniped species. The majority of these markers have broad utility in all pinnipeds and provide a strong base for detailed population genetic studies in the Pinnipedia.     

Integration of research and management in optimizing multiple uses of reservoirs: the experience in South America and Brazilian case studies

The construction of large reservoirs in South America and particularly in Brazil has intensified within the last 50 years. Built up primarily for hydroelectricity production, these artificial ecosystems now serve purposes such as: water storage for public use; fisheries and aquaculture; recreation; tourism, and irrigation. These artificial ecosystems were also built up with the purpose of enhancing the regional development. These activities produce multiple impacts among which are eutrophication, a serious problems with various ecological, economic, and social consequences. Basic studies on reservoirs have identified their main ecological characteristics and described some of their fundamental mechanisms of functioning. This information cover: factors involved in reservoir complexity, such as spatial scale, vertical and horizontal heterogeneity, temporal variation at several time scales and, in some cases, watershed/reservoir relationships, and the impact of non-point and point sources of nutrients. Numerous studies have been dedicated to describing aquatic biota, its relationship with environmental factors, and the impacts of degradation/pollution/ eutrophication on biodiversity. Research priorities have been established mainly by limnologists in response to needs identified by environmental impact assessment. Due to the pressure of multiple uses, population growth, and economic factors, aquatic scientists now face a number of questions being posed by managers and engineers. These questions relate to a wide range of practical problems, the solutions to which depend on accumulated data related to the structural complexities and function mechanisms previously referred to. These problems can be divided into three main groups: (i) eutrophication processes and their characteristics, and water quality control; (ii) impact of fisheries, aquaculture, and exotic species introduction on the biota and the water quality; (iii) sustainable development of reservoirs and optimization management of their multiple uses. Predictive capabilities required in the face of these problems should be based on modeling development and intensive use of data-bases resulting from long-term studies on reservoirs. The predictions thus made possible would involve economic factors and the costs of recovery of eutrophic reservoirs. The experience of reservoir research and management in South America and in Brazil is, probably part of a world wide tendency on reservoir research and development with the aim to optimize multiple uses. Based on the cooperation of limnologists and engineers, ecosystem management models must eventually be applied at the watershed / reservoir level, where demands on the part of managers and decision makers will certainly promote a predictive, integrated, and adaptive approach,based on fundamental research.     

Reproductive performance in grey seals: age-related improvement and senescence in a capital breeder

1. Three hypotheses have been advanced to account for age-related improvement in performance: the selection hypothesis predicts improved due to the loss of lower quality phenotypes, the constraint hypothesis predicts individuals improve function, and the restraint hypothesis predicts younger individuals forego or reduce effort because of mortality risks. A decline in age-related performance (i.e. senescence) is predicted by mutation accumulation, antagonistic pleiotropy and disposable soma (wear and tear) hypotheses. 2. Using five measures of performance - birth rate, maternal and pup birth mass, pup weaning mass, weaning success and lactation length - we tested these hypotheses concerning age-related change in reproduction in 279 female grey seals (Halichoerus grypus), ages 4-42 years, over a 23-year period between 1983 and 2005 on Sable Island, Nova Scotia. These females produced 2071 pups. 3. Although body mass of primiparous females increased with age (4-7 years) birth mass of their pups did not, but pup weaning mass did. Second- and third-parity females of the same age as primiparous females gave birth to and weaned heavier pups. However, parity and age were dropped from models when maternal body mass was included. 4. The proportion of females giving birth varied significantly with maternal age, increasing in young females and then declining late in life. Weaning success rate also increased rapidly to about 8 years and subsequently declined in females > 32 years. 5. Generalized additive models indicated nonlinear changes in 3 day body mass (i.e. approximately birth mass) and weaning mass of pups as a function of maternal age, after accounting statistically for the effects of maternal body mass. Mixed-effects, repeated-measures models fitted to longitudinal data further supported the conclusion that pup birth mass and weaning mass vary nonlinearly with maternal age and indicated nonlinear changes in lactation duration. 6. We found some support for the constraint hypothesis, but our findings were not consistent with the selection hypothesis or the restraint hypothesis as the basis for improvement in reproductive performance. 7. Senescence was evident in multiple female and offspring traits, indicating the degeneration in function of several physiological systems as predicted by the disposable soma hypothesis.     

Sex differences in grey seal diet reflect seasonal variation in foraging behaviour and reproductive expenditure: evidence from quantitative fatty acid signature analysis

1. Intraspecific variation in diet can be an important component of a species niche breadth. We tested the hypothesis that sex differences in seasonal foraging behaviour and energy storage of sexually size dimorphic grey seals Halichoerus grypus (Fabrisius 1971) are reflected in differences in the diet and niche breadth. Diet composition was estimated for 496 adult (226 males, 270 females) and 91 juvenile (46 males/45 females; all 6 months old) grey seals sampled between 1993 and 2000 using quantitative fatty acid signature analysis. Niche breadth and overlap were estimated using the Shannon-Weaver diversity index (H') and the Morisita-Horn index (C(H)), respectively. 2. Sand lance Ammodytes dubius (Reinhardt 1837) and redfish Sebastes sp. (Cuvier 1829) accounted for a high proportion of the diet in both sexes and age groups. However, the diets of adult males were significantly more diverse across all seasons (H': males 0.36 +/- 0.007 vs. females 0.28 +/- 0.007) and less energy dense in spring (male 5.3 +/- 0.07 kJ g(-1) vs. females 5.6 +/- 0.09 kJ g(-1)) than those of adult females. 3. Season and sex explained most of the observed variation in adult diets, but there were significant sex-season interactions. These differences were most evident during the post-breeding (spring) foraging period when energy acquisition is important to female recovery of nutrient stores needed to support pregnancy. Females selected fewer and higher quality prey species in spring than males. 4. There were no sex differences in the diets of juvenile grey seals. Although many of the species overlapped with those eaten by adults, juvenile niche breadth (H': 0.41 +/- 0.014, n = 91) was significantly broader than that of adults (H': 0.30 +/- 0.011, n = 115). Juvenile diets were also of lower energy density (5.3 +/- 0.04 kJ g(-1)) than those of adults (5.6 +/- 0.09 kJ g(-1)), suggesting less selectivity in these young and relatively na?ve predators. 5. Sex-specific seasonal changes in diet correspond to seasonal changes in diving behaviour and rate of body energy accumulation of adult males and females. Sex-specific reproductive requirements appear to be a primary factor generating the intraspecific variation in the seasonal foraging ecology of this large marine carnivore. However, sex differences in the breadth and energy content of diets also suggest the influence of body-size dimorphism as a factor shaping the diet of this species.     

国外旅游生态效率优化与管理

旅游生态效率概念采用定量化方法对旅游业经济、环境影响进行分析,成为旅游业可持续发展评价的重要工具。基于可持续理论的旅游生态效率优化管理方案不断涌现,相关利益主体应用较多的有环境管理系统、旅游生态标签、清洁生产理念、旅游生态效率中心、21世纪地方议程等。在旅游企业日常运营中积极推动生态效率优化管理,是贯彻绿色发展理念,提高目的地旅游经济、环境绩效的一种新思路。从可持续背景下旅游生态效率优化模型入手,系统化分析了国外旅游生态效率优化管理方案的内容与特征。其中,环境管理系统在目的地层面为旅游生态效率优化设计了一套评估管理流程,旅游生态标签为目的地生态效率水平提供了可视化标志符号,21世纪地方议程为旅游可持续发展提供一致性整合方案,清洁生产理念是一种基于生态效率优化的长期战略,旅游生态效率中心作为非营利性机构有利于中小企业获得较好的环境绩效表现。国外旅游生态效率优化管理方案特征明显、设计合理、管理科学,对我国目的地旅游生态效率优化提升具有重要的借鉴作用。     

Pleistocene population expansions of Antarctic seals

We sequenced a portion ( c . 475 bp) of the mitochondrial control region of three species of Antarctic phocid carnivores (Weddell seal, Leptonychotes weddellii , N  = 181; crabeater seal, Lobodon carcinophaga , N  = 143; and Ross seal, Ommatophoca rossii , N  = 41) that live seasonally or permanently in the fast ice and seasonal pack ice of the western Amundsen and Ross seas of western Antarctica. We resolved 251 haplotypes with a haplotype diversity of 0.98 to 0.99. Bayesian estimates of Θ from the program LAMARC ranged from 0.075 for Weddell seals to 0.576 for crabeater seals. We used the values of theta to estimate female effective population sizes ( N_EF ), which were 40 700 to 63 000 for Weddell seals, 44 400 to 97 800 for Ross seals, and 358 500 to 531 900 for crabeater seals. We used mismatch distributions to test for historical population size expansions. Weddell seals and crabeater seals had significant, unimodal mean pairwise difference distributions ( P  = 0.56 and 0.36, respectively), suggesting that their populations expanded suddenly around 731 000 years ago (Weddell seals) and around 1.6 million years ago (crabeater seals). Both of these expansions occurred during times of intensified glaciations and may have been fostered by expanding pack ice habitat.     

Bacteria used in the biological control of plant-parasitic nematodes: populations, mechanisms of action, and future prospects

As a group of important natural enemies of nematode pests, nematophagous bacteria exhibit diverse modes of action: these include parasitizing; producing toxins, antibiotics, or enzymes; competing for nutrients; inducing systemic resistance of plants; and promoting plant health. They act synergistically on nematodes through the direct suppression of nematodes, promoting plant growth, and facilitating the rhizosphere colonization and activity of microbial antagonists. This review details the nematophagous bacteria known to date, including parasitic bacteria, opportunistic parasitic bacteria, rhizobacteria, Cry protein-forming bacteria, endophytic bacteria and symbiotic bacteria. We focus on recent research developments concerning their pathogenic mechanisms at the biochemical and molecular levels. Increased understanding of the molecular basis of the various pathogenic mechanisms of the nematophagous bacteria could potentially enhance their value as effective biological control agents. We also review a number of molecular biological approaches currently used in the study of bacterial pathogenesis in nematodes. We discuss their merits, limitations and potential uses.     

Ten novel dinucleotide microsatellite loci cloned from the Galápagos sea lion (Zalophus californianus wollebaeki) are polymorphic in other pinniped species

We isolated and characterized 10 novel dinucleotide microsatellite loci from the Galápagos sea lion (Zalophus californianus wollebaeki) and tested their amplification utility in four further otariid species (Zalophus californianus californianus, Arctocephalus gazella, Arctocephalus australis and Eumetopias jubatus) and three phocid species (Hydrurga leptonyx, Halichoerus grypus and Phoca vitulina). All of the loci amplified polymorphic polymerase chain reaction (PCR) products in at least three species other than the Galápagos sea lion. These markers will be useful for studies of pinniped mating systems, genetic structure and genetic diversity.