共查询到20条相似文献,搜索用时 31 毫秒
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Background
Recent work has identified that many long mRNA-like noncoding RNAs (lncRNAs) are expressed in the developing nervous system. Despite their abundance, the function of these ncRNAs has remained largely unexplored. We have investigated the highly abundant lncRNA RNCR2 in regulation of mouse retinal cell differentiation. 相似文献2.
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Stefan E Seemann Andreas S Richter Jan Gorodkin Rolf Backofen 《Algorithms for molecular biology : AMB》2010,5(1):22
Background
Many regulatory non-coding RNAs (ncRNAs) function through complementary binding with mRNAs or other ncRNAs, e.g., microRNAs, snoRNAs and bacterial sRNAs. Predicting these RNA interactions is essential for functional studies of putative ncRNAs or for the design of artificial RNAs. Many ncRNAs show clear signs of undergoing compensating base changes over evolutionary time. Here, we postulate that a non-negligible part of the existing RNA-RNA interactions contain preserved but covarying patterns of interactions. 相似文献4.
Wenjie Shu Xiaochen Bo Rujia Liu Dongsheng Zhao Zhiqiang Zheng Shengqi Wang 《BMC bioinformatics》2006,7(1):404-7
Background
The diverse functions of ncRNAs critically depend on their structures. Mutations in ncRNAs disrupting the structures of functional sites are expected to be deleterious. RNA deleterious mutations have attracted wide attentions because some of them in cells result in serious disease, and some others in microbes influence their fitness. 相似文献5.
Background
Non-coding RNAs (ncRNAs) have a multitude of roles in the cell, many of which remain to be discovered. However, it is difficult to detect novel ncRNAs in biochemical screens. To advance biological knowledge, computational methods that can accurately detect ncRNAs in sequenced genomes are therefore desirable. The increasing number of genomic sequences provides a rich dataset for computational comparative sequence analysis and detection of novel ncRNAs. 相似文献6.
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Long noncoding RNAs with enhancer-like function in human cells 总被引:5,自引:0,他引:5
Ørom UA Derrien T Beringer M Gumireddy K Gardini A Bussotti G Lai F Zytnicki M Notredame C Huang Q Guigo R Shiekhattar R 《Cell》2010,143(1):46-58
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Background
Recent discoveries of a large variety of important roles for non-coding RNAs (ncRNAs) have been reported by numerous researchers. In order to analyze ncRNAs by kernel methods including support vector machines, we propose stem kernels as an extension of string kernels for measuring the similarities between two RNA sequences from the viewpoint of secondary structures. However, applying stem kernels directly to large data sets of ncRNAs is impractical due to their computational complexity. 相似文献13.
Dong Jia Lun Cai Housheng He Geir Skogerbø Tiantian Li Muhammad Nauman Aftab Runsheng Chen 《BMC molecular biology》2007,8(1):86
Background
The 2,2,7-trimethylguanosine (TMG) cap structure is an important functional characteristic of ncRNAs with critical cellular roles, such as some snRNAs. Here we used immunoprecipitation with both K121 and R1131 anti-TMG antibodies to systematically identify the TMG cap structures for all presently characterized ncRNAs in C. elegans. 相似文献14.
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Susan DJ Chapple Anna M Crofts S Paul Shadbolt John McCafferty Michael R Dyson 《BMC biotechnology》2006,6(1):49-15
Background
A variety of approaches to understanding protein structure and function require production of recombinant protein. Mammalian based expression systems have advantages over bacterial systems for certain classes of protein but can be slower and more laborious. Thus the availability of a simple system for production and rapid screening of constructs or conditions for mammalian expression would be of great benefit. To this end we have coupled an efficient recombinant protein production system based on transient transfection in HEK-293 EBNA1 (HEK-293E) suspension cells with a dot blot method allowing pre-screening of proteins expressed in cells in a high throughput manner. 相似文献18.
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Testis-expressed profilins 3 and 4 show distinct functional characteristics and localize in the acroplaxome-manchette complex in spermatids 总被引:1,自引:0,他引:1
Martina Behnen Kai Murk Petri Kursula Heike Cappallo-Obermann Martin Rothkegel Abraham L Kierszenbaum Christiane Kirchhoff 《BMC cell biology》2009,10(1):34-16
Background
Multiple profilin isoforms exist in mammals; at least four are expressed in the mammalian testis. The testis-specific isoforms profilin-3 (PFN3) and profilin-4 (PFN4) may have specialized roles in spermatogenic cells which are distinct from known functions fulfilled by the "somatic" profilins, profilin-1 (PFN1) and profilin-2 (PFN2). 相似文献20.
Marissa A Bowden Ying Li Yi-Xun Liu Jock K Findlay Lois A Salamonsen Guiying Nie 《Reproductive biology and endocrinology : RB&E》2008,6(1):22