Purification of protease-extracted glycosaminoglycans by short-distance paper chromatography with a zinc acetate solvent

A method for the purification of tissue-extracted glycosaminoglycans, using short-distance (5-cm path) paper chromatography with 0.25 m zinc acetate solvent, pH 5.0, is presented. The method removed 96 to 97% of the impurities present in the original tissue extract, based on absorbance at 260 nm and protein and phosphate contents. Recovery of control samples of dermatan sulfate averaged 88%. In place of zinc, manganous and mercuric acetates were used to test the method; however, zinc was found to be preferable.     

A new solvent system for the separation of neutral glycosphingolipids

A solvent system and a column for high performance liquid chromatography for the separation of glycosphingolipids without derivatization is described. A column pakced with porous silica gel (latrobeads) and eluted with a mixture of isopropanol-hexane-water with increasing water content and decreasing hexane content was used. Glycosphingolipids with mono- to dodeca- or tetrakaidecasaccharides were separated within 60 min and the separation pattern was highly reproducible. The method was applied for preparative separation of highly complex glycolipids with blood group activity.     

Use of solvent mixtures for total lipid extraction of Chlorella vulgaris and gas chromatography FAME analysis

Bioprocess and Biosystems Engineering - Lipid extraction is the bottleneck step for algae-based biodiesel production. Herein, 12 solvent mixture systems (mixtures of three non-polar and two polar...