Stonin 2 is a major adaptor protein for clathrin-mediated synaptic vesicle retrieval

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Highlights? Targeted RNAi screen of adaptors for vesicle retrieval at hippocampal synapses ? Stonin 2 is a major adaptor for clathrin-mediated retrieval of synaptic vesicles ? Rapid, induced rerouting of stonin 2 demonstrates the role of this adaptor     

The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain

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Highlights? ESCRT-I subunit UBAP1 is essential for degradation of antiviral protein tetherin ? UBAP1 has a domain consisting of a solenoid of overlapping UBAs (SOUBA) ? Each of the three UBAs in the SOUBA binds monoubiquitin     

Adaptor protein self-assembly drives the control of a cullin-RING ubiquitin ligase

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Highlights? The crystal structure of the heterotetrameric CRL3-SPOP ubiquitin ligase is reported ? Substrate adaptor proteins are recruited to CRL complexes through a signature motif ? Tandem BTB and BACK domains potentiate assembly and activity of the CRL3-SPOP ligase ? The SPOPL negative regulator creates a molecular rheostat to fine-tune CRL3 activity     

Structural Basis of Selective Ubiquitination of TRF1 by SCFFbx4

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Highlights? SCF ubiquitin ligase subunit Fbx4 binds TRF1 using an atypical small GTPase fold ? Fbx4 recognizes a globular domain of TRF1, not just short phosphorylated degrons ? Telomere shelterin component TIN2 competes with Fbx4 for TRF1 binding ? TIN2 and SCF^Fbx4 thus control TRF1 ubiquitination and degradation     

CRL1-FBXO11 Promotes Cdt2 Ubiquitylation and Degradation and Regulates Pr-Set7/Set8-Mediated Cellular Migration

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Highlights? Cullin 4A promotes the autoubiquitylation of Cdt2, a substrate adaptor of CRL4^Cdt2 ? CRL1^FBXO11 also ubiquitylates and promotes the degradation of Cdt2 ? FBXO11 degrades Cdt2 to stabilize Set8 and regulate the response to TGF-β ? FBXO11 is similarly required to stabilize Set8 and promote cell migration     

Dynamin 2 Potentiates Invasive Migration of Pancreatic Tumor Cells through Stabilization of the Rac1 GEF Vav1

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Highlights? Dyn2 promotes lamellipodia formation and pancreatic tumor cell migration ? Direct binding between Dyn2 and Vav1 promotes Rac1 activation and migration ? Dyn2 binding protects Vav1 from degradation by the lysosome ? Vav1 is targeted for degradation through an interaction with Hsc70     

The Crystal Structures of the Eukaryotic Chaperonin CCT Reveal Its Functional Partitioning

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Highlights? CCT subunit types can be identified in crystallographic data, even at low resolutions ? Actin and tubulin both bind around CCT6 ? ATP hydrolysis and substrate binding are partitioned in the CCT particle     

piRNA-Triggered MIWI Ubiquitination and Removal by APC/C in Late Spermatogenesis

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Highlights? MIWI is a substrate of APC/C, and piRNA loading is essential for MIWI ubiquitination ? piRNA loading promotes MIWI binding to the APC/C substrate-binding subunit ? MIWI and piRNAs are coordinately eliminated in late spermatids ? Inhibition of MIWI destruction in late spermatids prevents sperm maturation     

Regulation of the CRL4Cdt2 Ubiquitin Ligase and Cell-Cycle Exit by the SCFFbxo11 Ubiquitin Ligase

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Highlights? FBXO11 targets CDT2, a CRL4 substrate receptor, for proteasomal degradation ? CDK-mediated phosphorylation of CDT2 degron inhibits recognition by FBXO11 ? FBXO11-mediated degradation of CDT2 controls the timing of cell-cycle exit ? FBXO11-CDT2 functional interaction is evolutionary conserved from worms to humans     

Insights into FlaI Functions in Archaeal Motor Assembly and Motility from Structures,Conformations, and Genetics

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Highlights? Cross-subunit interface forms building block for transmission of ATPase force ? Structures and mutants reveal functional components for assembly and motility ? The directionality of movement explains FlaI activities and GspE differences ? The phosphate released by ATP hydrolysis triggers subunit rearrangements     

A unique spatial arrangement of the snRNPs within the native spliceosome emerges from in silico studies

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Highlights? We present a computational procedure for fast matching of thousands of conformers ? We obtained a unique model localizing the snRNPs within the native spliceosome ? The 5 snRNPs are accommodated mostly within the large subunit of the spliceosome ? The large spliceosomal cavity emerges as the site of mRNA binding and splicing     

Recruitment of UBPY and ESCRT Exchange Drive HD-PTP-Dependent Sorting of EGFR to the MVB

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Highlights? HD-PTP is essential for sorting EGFR to the MVB lumen ? HD-PTP binds the ESCRT-0 subunit STAM2 at two sites ? CHMP4B and UBPY displace EGFR from ESCRT-0 and drive ESCRT-III association ? UBPY is important for sorting EGFR to the MVB lumen     

Structure and mechanism of the CMR complex for CRISPR-mediated antiviral immunity

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Highlights? EM structure of the CMR complex for viral RNA degradation has been determined ? The crRNA content of CMR has been analyzed by deep sequencing ? Target RNA cleavage by CMR is sequence dependent     

Structure and 3D Arrangement of Endoplasmic Reticulum Membrane-Associated Ribosomes

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Highlights? Subtomogram average of the canine ER-associated ribosome in situ at 31 Å resolution ? Large subunit rRNA ES27L is in direct contact with the ER membrane ? Sec61, TRAP, and potentially OST and the SP complex are resolved ? ER-associated ribosomes adopt a preferred arrangement, likely polyribosome specific     

Repression of the Long Noncoding RNA-LET by Histone Deacetylase 3 Contributes to Hypoxia-Mediated Metastasis

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Highlights? There is low expression of lncRNA-LET in various tumor tissues ? Hypoxia-induced low expression of lncRNA-LET occurred through histone deacetylase 3 ? The lncRNA-LET is bound to NF90, which increases NF90 degradation by the proteasome ? Low lncRNA-LET expression contributes to hypoxia-induced cell invasion     

DNA Minor Groove Sensing and Widening by the CCAAT-Binding Complex

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Highlights? CBC binds to the minor groove of the DNA and bends it in a nucleosome-like manner ? Interactions of subunit HapB with DNA explain the sequence specificity of the CBC ? Anchor A of HapB tethers the CBC to the CCAAT box by minor groove widening ? Interactions of the CBC with the histones H3/H4 of the nucleosome are modeled     

Structural Characterization of a Mouse Ortholog of Human NEIL3 with a Marked Preference for Single-Stranded DNA

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Highlights? Neil3 is an Fpg/Nei DNA glycosylase of the base excision repair pathway ? Neil3 excises a broad spectrum of base lesions, with a marked preference for ssDNA ? We report a crystal structure of Neil3 DNA glycosylase ? The structure provides valuable insights into Neil3′s unusual substrate specificity