共查询到20条相似文献,搜索用时 31 毫秒
1.
A quantitative trait locus for reduced culm internode length in barley segregates as a Mendelian gene 总被引:1,自引:0,他引:1
M. Sameri S. Nakamura S. K. Nair K. Takeda Takao Komatsuda 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(4):643-652
Yield losses caused by lodging in cereals can be partially controlled by reducing plant height. A progeny of recombinant inbred
lines from a cross of two Japanese barley varieties was used to study the inheritance of culm and culm internode lengths.
An unexpected QTL for reduced culm length (qCUL), which affected mainly the length of the third and fourth culm internodes, was contributed by ‘Kanto Nakate Gold’. This
QTL was also associated with reduced lodging in two experiments. A near-isogenic line (culm length 62.9–73.4 cm) in an ‘Azumamugi’
background, carrying a chromosome segment containing the qCUL allele from Kanto Nakate Gold, was significantly shorter than its recurrent parent (82.9–89.4 cm). The F2 generation from the next backcross segregated for plant height in a Mendelian monogenic ratio. The qCUL locus was shown to be tightly linked (1.2 cM) with the codominant STS marker ABG608.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
2.
Mammadov JA Steffenson BJ Maroof MA 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(8):1651-1660
The rapidly growing expressed sequence tag (EST) resources of species representing the Poacea family and availability of comprehensive sequence information for the rice (Oryza sativa) genome create an excellent opportunity for comparative genome analysis. Extensive synteny between rice chromosome 1 and
barley (Hordeum vulgare L.) chromosome 3 has proven extremely useful for saturation mapping of chromosomal regions containing target genes of large-genome
barley with conserved orthologous genes from the syntenic regions of the rice genome. Rph5 is a gene conferring resistance to the barley leaf rust pathogen Puccinia hordei. It was mapped to chromosome 3HS, which is syntenic with rice chromosome 1S. The objective of this study was to increase
marker density within the sub-centimorgan region around Rph5, using sequence-tagged site (STS) markers that were developed based on barley ESTs syntenic to the phage (P1)-derived artificial
chromosome (PAC) clones comprising the distal region of rice chromosome 1S. Five rice PAC clones were used as queries in a
blastn search to screen 375,187 barley ESTs. Ninety-four non-redundant EST sequences were identified from the EST database
and used as templates to design 174 pairs of primer combinations. As a result, 9 barley EST-based STS markers were incorporated
into the ‘Bowman’ × ‘Magnif 102’ high-resolution map of the Rph5 region. More importantly, six markers, including five EST-derived STS sequences, were found to co-segregate with Rph5. The results of this study demonstrate the usefulness of rice genomic resources for efficient deployment of barley ESTs for
marker saturation of targeted barley genomic regions. 相似文献
3.
A genetic linkage map of tef [Eragrostis tef (Zucc.) Trotter] based on amplified fragment length polymorphism 总被引:2,自引:0,他引:2
G. Bai H. Tefera M. Ayele H. T. Nguyen 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(3-4):599-604
A genetic linkage map of tef was constructed with amplified fragment length polymorphism (AFLP) markers using F5 recombinant inbred lines (RILs) derived by single seed descent from the intraspecific cross of ’Kaye Murri’×’Fesho’. A total
of 192 EcoRI/MseI primer combinations were screened for parental polymorphism. Around three polymorphic fragments per primer combination were
detected, indicating a low polymorphism level in tef. Fifty primer combinations were selected to assay the mapping population,
and 226 loci segregated among 85 F5 RILs. Most AFLP loci behaved as dominant markers (presence or absence of a band), but about 15% of the loci were codominant.
Significant deviations from the expected Mendelian segregation ratio were observed for 26 loci. The genetic linkage map comprised
211 markers assembled into 25 linkage groups and covered 2,149 cM of genome. AFLP is an efficient marker system for mapping
plant species with low polymorphism such as tef. This is the first genetic linkage map constructed for tef. It will facilitate
the mapping of genes controlling agronomically important traits and cultivar improvement in tef.
Received: 27 April 1998 / Accepted: 4 January 1999 相似文献
4.
J. K. Roy M. Prasad R. K. Varshney H. S. Balyan T. K. Blake H. S. Dhaliwal H-Singh K. J. Edwards P. K. Gupta 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):336-340
In bread wheat, the transfer of tolerance to preharvest sprouting (PHS) that is associated with genotypes having red kernel
colour to genotypes with amber kernels is difficult using conventional methods of plant breeding. The study here was undertaken
to identify DNA markers linked with tolerance to PHS as these would allow indirect marker-assisted selection of PHS-tolerant
genotypes with amber kernels. For this purpose, a set of 100 recombinant inbred lines (RILs) was developed using a cross between
a PHS-tolerant genotype, SPR8198, with red kernels and a PHS-susceptible cultivar, ‘HD2329’, with white kernels. The two parents
were analysed with 232 STMS (sequence-tagged microsatellite site) and 138 STS (sequence-tagged site) primer pairs. A total
of 300 (167 STMSs and 133 STSs) primer pairs proved functional by giving scorable PCR products. Of these, 57 (34%) STMS and
30 (23%) STS primer pairs detected reproducible polymorphism between the parent genotypes. Using these primer pairs, we carried
out bulked segregant analysis on two bulked DNAs, one obtained by pooling DNA from 5 PHS-tolerant RILs and the other similarly
derived by pooling DNA from 5 PHS-susceptible RILs. Two molecular markers, 1 STMS primer pair for the locus wmc104 anda STS primer pair for the locus MST101, showed apparent linkage with tolerance to PHS. This was confirmed following selective genotyping of individual RILs included
in the bulks. Chi-square contingency tests for independence were conducted on the cosegregation data collected on 100 RILs
involving each of the two molecular markers (wmc104 and MST101) and PHS. The tests revealed a strong association between each of the markers and tolerance to PHS. Using nullisomic-tetrasomic
lines, we were able to assign wmc104 and MST101 to chromosomes 6B and 7D, respectively. The results also indicated that the tolerance to PHS in SPR8198 is perhaps governed
by two genes (linked with two molecular markers) exhibiting complementary interaction.
Received: 15 October 1998 / Accepted: 19 December 1998 相似文献
5.
Molecular mapping of the blast resistance gene, Pi44(t), in a line derived from a durably resistant rice cultivar 总被引:12,自引:0,他引:12
D.-H. Chen M. dela Viña T. Inukai D. J. Mackill P. C. Ronald R. J. Nelson 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(6-7):1046-1053
A recombinant inbred line derived from a cross between CO39 and ‘Moroberekan’, RIL276, was found to be resistant to lineage
44 isolates of Pyricularia grisea in the Philippines. One hundred F2 individuals were obtained from a backcross of RIL276 and CO39. Phenotypic analysis showed that RIL276 carries a single locus,
tentatively named Pi44(t), conferring complete resistance to lineage 44 isolates of P. grisea. RFLP probes, STS primers and AFLP markers were applied to identify DNA markers linked to Pi44(t). Neither RFLP nor STS-PCR analysis gave rise to DNA markers linked to the locus. Using bulk segregant AFLP analysis, however,
two dominant AFLP markers (AF348 and AF349) linked to Pi44(t) were identified. AF349 and AF348 were located at 3.3±1.5 cM and 11±3.5 cM from Pi44(t), respectively. These markers were mapped on chromosome 11 using an F2 population derived from a cross between ‘Labelle’ and ‘Black Gora’. The location of AF348 on chromosome 11 was confirmed using another F2 mapping population derived from IR40931-26-3-3-5/ PI543851. DNA products at the loci linked to Pi44(t) were amplified from RIL276, ‘Labelle’ and PI543851 using the same primer pairs used to amplify AF349 and AF348. Sequence analysis of these bands showed 100% identity between lines. This result indicates that these AFLP markers could
be used for the comparison of maps or assignment of linkage groups to chromosomes.
Received: 12 May 1998 / Accepted: 13 November 1998 相似文献
6.
A linkage map of the pea (Pisum sativum L.) genome containing cloned sequences of known function and expressed sequence tags (ESTs) 总被引:3,自引:0,他引:3
B. J. Gilpin J. A. McCallum T. J. Frew G. M. Timmerman-Vaughan 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(8):1289-1299
A linkage map of the pea (Pisum sativum L.) genome is presented which is based on F2 plants produced by crossing the marrowfat cultivar ‘Primo’ and the blue-pea breeding line ‘OSU442-15’. This linkage map consists
of 209 markers and covers 1330 cM (Kosambi units) and includes RFLP, RAPD and AFLP markers. By mapping a number of anchor
loci, the ‘Primo’בOSU442-15’ map has been related to other pea linkage maps. A feature of the map is the incorporation of
29 loci representing genes of known function, obtained from other laboratories. The map also contains RFLP loci detected using
sequence-characterized cDNA clones developed in our laboratory. The putative identities of 38 of these cDNA clones were assigned
by examining public-sequence databases for protein or nucleotide-sequence similarities. The conversion of sequence-characterized
pea cDNAs into PCR-amplifiable and polymorphic sequence-tagged sites (STSs) was investigated using 18 pairs of primers designed
for single-copy sequences. Eleven polymorphic STSs were developed.
Received: 18 June 1997 / Accepted: 11 August 1997 相似文献
7.
Genome scanning for resistance-gene analogs in rice, barley, and wheat by high-resolution electrophoresis 总被引:31,自引:8,他引:23
X. M. Chen R. F. Line H. Leung 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(3):345-355
Genes cloned from diverse plants for resistance to different pathogens have sequence similarities in domains presumably involved
in pathogen recognition and signal transduction in triggering the defense response. Primers based on the conserved regions
of resistance genes often amplify multiple fragments that may not be separable in an agarose gel. We used denaturing polyacrylamide-gel
electrophoresis to detect PCR products of plant genomic DNA amplified with primers based on conserved regions of resistance
genes. Depending upon the primer pairs used, 30–130 bands were detected in wheat, rice, and barley. As high as 47%, 40%, and
27% of the polymorphic bands were detected in rice, barley, and wheat, respectively, and as high as 12.5% of the polymorphic
bands were detected by certain primers in progeny from a cross of the wheat cultivars ‘Stephens’ and ‘Michigan Amber’. Using
F6 recombinant inbred lines from the ‘Stephens’בMichigan Amber’ cross, we demonstrated that polymorphic bands amplified with
primers based on leucine-rich repeats, nucleotide-binding sites and protein kinase genes, were inherited as single loci. Linkages
between molecular markers and stripe rust resistance genes were detected. This technique provides a new way to develop molecular
markers for assessing the genetic diversity of germplasm based upon potential candidate resistance genes in diverse species.
Received : 5 September 1997 / Accepted : 6 November 1997 相似文献
8.
Transfer of mapping information between related species has facilitated the development of restriction fragment length polymorphism (RFLP) maps in the cereals. Sequence tagged site (STS) primer sets for use in the polymerase chain reaction may be developed from mapped RFLP clones. For this study, we mapped 97 STS primer sets to chromosomes in wheat and barley to determine the potential transferability of the primer sets and the degree of correspondence between RFLP and STS locations. STS products mapped to the same chromosome group in wheat and barley 75% of the time. RFLP location predicted STS location 69% of the time in wheat and 56% of the time in barley. Southern hybridizations showed that most primer sets amplified sequences homologous to the RFLP clone, although additional sequences were often amplified that did not hybridize to the RFLP clone. Nontarget sequences were often amplified when primer sets were transferred across species. In general, results suggest a good probability of success in transferring STSs between wheat and barley, and that RFLP location can be used to predict STS location. However, transferability of STSs cannot be assumed, suggesting a need for recombinational mapping of STS markers in each species as new primer sets are developed. Key words : sequence tagged sites, PCR, wheat, barley. 相似文献
9.
G. Schwarz W. Michalek V. Mohler G. Wenzel A. Jahoor 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(3-4):521-530
The complex Mla locus of barley determines resistance to the powdery mildew pathogen Erysiphe graminis f. sp. hordei. With a view towards gene isolation, a population consisting of 950 F2 individuals derived from a cross between the near-isogenic lines ‘P01’ (Mla1) and ‘P10’ (Mla12) was used to construct a high-resolution map of the Mla region. A fluorescence-based AFLP technique and bulked segregant analysis were applied to screen for polymorphic, tightly
linked AFLP markers. Three AFLP markers were selected as suitable for a chromosome-landing strategy. One of these AFLP markers
and a closely linked RFLP marker were converted into sequence-specific PCR markers. PCR-based screening of approximately 70 000
yeast artificial chromosome (YAC) clones revealed three identical YACs harbouring the Mla locus. Terminal insert sequences were obtained using inverse PCR. The derived STS marker from the right YAC end-clone was
mapped distal to the Mla locus.
Received: 17 July 1998 / Accepted: 9 August 1998 相似文献
10.
K. J. Williams A. Lichon P. Gianquitto J. M. Kretschmer A. Karakousis S. Manning P. Langridge H. Wallwork 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):323-327
Spot form of net blotch (SFNB) (Pyrenophora teres f maculata) is an economically damaging foliar disease of barley in many of the world’s cereal growing areas. The development of SFNB-resistant
cultivars may be accelerated through the use of molecular markers. A screen for SFNB resistance in 96 lines identified four
new sources of resistance, including a feed variety, ‘Galleon’, for which a fully mapped doubled haploid population was available.
Segregation data indicated SFNB resistance was conferred by a single gene in the ‘Galleon’בHaruna Nijo’ cross, positioned
on the long arm of chromosome 7H. This gene is designated Rpt4 and is flanked by the RFLP loci Xpsr117(D) and Xcdo673 at distances of 6.9 cM and 25.9 cM, respectively. The marker Xpsr117(D) was validated using another population segregating for Rpt4, correctly predicting SFNB resistance with more than 90% accuracy.
Received: 24 September 1998 / Accepted: 19 December 1998 相似文献
11.
J. M. Kretschmer K. J. Chalmers S. Manning A. Karakousis A. R. Barr A. K. M. R. Islam S. J. Logue Y. W. Choe S. J. Barker R. C. M. Lance P. Langridge 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(8):1060-1064
The cereal cyst nematode (CCN), Heterodera avenae Woll., is an economically damaging pest of barley in many of the world’s cereal-growing areas. The development of CCN-resistant
cultivars may be accelerated through the use of molecular markers. A number of resistance genes against the pest are well
known; one of them, the single dominant Ha 2 resistance gene, has been shown to be effective against the Australian pathotype and maps to chromosome 2 of barley. Segregation
analysis identified two restriction fragment length polymorphism (RFLP) markers flanking the resistance gene in two doubled-haploid
populations of barley. AWBMA 21 and MWG 694 mapped 4.1 and 6.1 cM respectively from the Ha 2 locus in the Chebec×Harrington cross and 4.0 and 9.2 cM respectively in the Clipper×Sahara cross. Analysis of a further seven
sources of CCN resistance in the form of near-isogenic lines (NILs) indicates that all available sources of resistance to
the Australian pathotype of CCN in barley represent the Ha 2 locus.
Received: 5 December 1996 / Accepted: 20 December 1996 相似文献
12.
V. Ivandic S. Malyshev V. Korzun A. Graner A. Börner 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(5):728-731
We report the genetic mapping of Dwf2, a dominant gibberellic acid (GA3)-insensitive dwarfing gene which has been previously described to cause a very short growth habit in barley (Hordeum vulgare) mutant ‘93/B694’. Using RFLP and microsatellite markers we performed segregation analysis in an F2 population comprising 86 individuals developed from a cross of ‘93/B694’ (Dwf2) with ‘Bonus M2’ (dwf2). Dwf2 was mapped on the short arm of barley chromosome 4H proximal to microsatellite marker XhvOle (5.7 cM) and distal to RFLP marker Xmwg2299 (18.3 cM). The genetic localization of the Dwf2 gene at a homoeologous position to the multiallelic Rht-B1 and Rht-D1 loci in wheat suggests synteny of GA-insensitive dwarfing genes within the Triticeae. Moreover, the extremely prostrate growth habit exhibited in barley ‘93/B694’ (Dwf2) resembles that of wheat plants carrying the genes Rht-B1c (Rht3) or Rht-D1c (Rht10).
Received: 1 July 1998 / Accepted: 17 September 1998 相似文献
13.
Mano Y Komatsuda T 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,105(5):708-715
Quantitative trait loci (QTLs) controlling callus growth (CG), subsequent shoot differentiation ratio (SD) and green shoot ratio (GS) in immature embryo culture were identified in barley. A base map was developed from 99 recombinant inbred lines (RILs) of 'Azumamugi' 2 'Kanto Nakate Gold'. The tissue-culture traits were evaluated at the F7 and F10 generations of the RILs. The RILs showed wide and continuous variations in each of the three tissue-culture traits. Three QTLs for CG, three QTLs for SD and two QTLs for GS were detected by using composite interval mapping. A QTL for SD on chromosome 3H had a large effect, and 'Kanto Nakate Gold', which has a high differentiation ability, contributed to this QTL. The location of this QTL is identical to, or very close to, the uzu locus. We discuss the relationships between tissue-culture loci in 'Azumamugi' 2 'Kanto Nakate Gold' and those in other mapping populations. 相似文献
14.
D. Milbourne R. C. Meyer A. J. Collins L. D. Ramsay C. Gebhardt R. Waugh 《Molecular & general genetics : MGG》1998,259(3):233-245
Solanum tuberosum L. DNA sequences containing simple sequence repeat (SSR) motifs were extracted from the EMBL database, cDNA and selectively
enriched small-insert DNA libraries. Enrichment was achieved using either triplex affinity capture or single-strand hybridisation
selection. One hundred and twelve primer pairs which successfully amplified products of the correct size from potato DNA were
ultimately designed and synthesised. Ninety-eight of these revealed length polymorphisms in a panel of four diploid and two
tetraploid clones, in agreement with the high information content of this class of markers which has been found in other species.
All of the markers were assigned a quality score of 1–5 based on their potential usefulness. Eighty-nine loci from 65 of the
primer pairs were located on two genetic linkage maps of potato by segregation analysis of the amplified alleles. Fifty-two
of the SSRs were clearly single locus. The maps were aligned using 23 SSR primer pairs and 13 RFLP loci mapped in both populations.
The markers described constitute a class which should replace Restriction Fragment Length Polymorphisms (RFLP) as the markers
of choice for future genetic studies in potato. The sequences of the primers, together with other information on these markers
are provided.
Received: 12 January 1998 / Accepted: 25 March 1998 相似文献
15.
A bacterial artificial chromosome library for soybean and identification of clones near a major cyst nematode resistance gene 总被引:11,自引:0,他引:11
D. Danesh S. Peñuela J. Mudge R. L. Denny H. Nordstrom J. P. Martinez N. D. Young 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(2):196-202
We constructed a bacterial artificial chromosome (BAC) library for soybean (Glycine max) consisting of approximately 30 000 clones with an average insert size of 120 kilobase pairs. The library was successfully
screened with restriction fragment length polymorphism (RFLP) and microsatellite markers tightly linked to a major resistance
gene for the cyst nematode, Heterodera glycines. Since many soybean RFLPs hybridize to duplicate loci, BACs homologous to duplicate RFLP loci were distinguished by digestion
with the restriction enzyme originally used to map the RFLP, followed by a comparison of the hybridizing fragments. Linkage
mapping of BAC clones identified with markers linked to the cyst nematode resistance gene demonstrated that these clones were
located at the expected chromosomal positions and that there were no indications of chimeras within the genomic inserts.
Received: 3 July 1997/Accepted: 26 August 1997 相似文献
16.
The locus Shd1, which we previously mapped to the long arm of chromosome 2 of Hordeum vulgare L., controls the differentiation of shoots from immature barley embryo callus. The locus has major effects and its action explains more than 65% of the total genetic variance in the shoot-differentiation rate. The allele of cultivar Kanto Nakate Gold designated Shd1K has a significant positive effect on the shoot-differentiation rate, whereas Shd1A of cultivar Azumamugi does not promote shoot differentiation. To identify gene products and characterize the function of Shd1, a set of near-isogenic lines is essential. In this study we produced BC5F1 plants by repeated backcrossing of 'Azumamugi' to F1 plants ('Azumamugi' x 'Kanto Nakate Gold'). The BC5F1 plants were examined for their RFLP genotype and for the shoot-differentiation ability of immature embryo-derived callus. The results indicated that the Shd1 locus was located in a chromosomal region between MWG2081 and MWG503 that flanks the MWG801, cMWG699, v (ear type), and MWG865 loci. Shd1K from 'Kanto Nakate Gold' functions effectively in the genetic background of 'Azumamugi', an indication that backcross breeding is possible for production of near-isogenic lines that would be very suitable for tissue culture. 相似文献
17.
Shen B Wang DM McIntyre CL Liu CJ 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(8):1489-1494
A bacterial artificial chromosome (BAC) library was constructed from the bread wheat (Triticum aestivum L.) genotype ‘Chinese Spring’ (‘CS’). The library consists of 395,136 clones with an estimated average insert size of 157 kb.
This library provides an estimated 3.4-fold genome coverage for this hexaploid species. The genome coverage was confirmed
by RFLP analysis of single-copy RFLP clones. The CS BAC library was used to develop simple sequence repeat (SSR) markers for
targeted genome regions using five sequence-tagged-site (STS) markers designed from the chromosome arm of 3BS. The SSR markers
for the targeted genome region were successfully obtained. However, similar numbers of new SSR markers were also generated
for the other two homoeologous group 3 chromosomes. This data suggests that BAC clones belonging to all three chromosomes
of homoeologous group 3 were isolated using the five STS primers. The potential impacts of these results on marker isolation
in wheat and on library screening in general are discussed. 相似文献
18.
M. Mohan P. V. Sathyanarayanan A. Kumar M. N. Srivastava S. Nair 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(5-6):777-782
A PCR-based marker (E20570) linked to the gene Gm4t, which confers resistance to a dipteran pest gall midge (Orseolia oryzae), has been mapped using the restriction fragment length polymorphism (RFLP) technique in rice. Gm4t is a dominant resistance gene. We initially failed to detect useful polymorphism for this marker in a F3 mapping population derived from a cross between two indica parents, ‘Abhaya’בShyamala’, with as many as 35 restriction enzymes. ‘Abhaya’ carries the resistance gene Gm4t and ‘Shyamala’ is susceptible to gall midge. Subsequently, E20570 was mapped using another mapping population represented by a F2 progeny from a cross between ‘Nipponbare’, a japonica variety, and ‘Kasalath’, an indica variety, in which the gene Gm4t was not known to be present. Gm4t mapped onto chromosome 8 between markers R1813 and S1633B. Our method, thus, presents an alternative way of mapping genes
which otherwise would be difficult to map because of a lack of polymorphism between closely related parents differing in desired
agronomic traits.
Received: 1 April 1997 / Accepted: 13 May 1997 相似文献
19.
X. Wang R. Trigiano M. Windham B. Scheffler T. Rinehart J. Spiers 《Tree Genetics & Genomes》2008,4(3):461-468
Abundant, codominant simple sequence repeats (SSRs) markers can be used for constructing genetic linkage maps and in marker-assisted
breeding programs. Enrichment methods for SSR motifs were optimized with the ultimate aim of developing numerous loci in flowering
dogwood (C. florida L.) genome. Small insert libraries using four motifs (GT, CT, TGG, and AAC) were constructed with C. florida ‘Cherokee Brave’ deoxyribonucleic acid (DNA). Colony polymerase chain reaction (PCR) of 2,208 selected clones with three
primers we reported previously indicated that 47% or 1,034 of the clones harbored one of the four targeted SSR motifs. Sequencing
the putative positive clones confirmed that nearly 99% (1,021 of 1,034) of them contained the desired motifs. Of the 871 unique
SSR loci, 617 were dinucleotide repeats (70.8%), and 254 were trinucleotide or longer repeats (29.2%). In total, 379 SSR loci
had perfect structure, 237 had interrupted, and 255 had compound structure. Primer pairs were designed from 351 unique sequences.
The ability of the 351 SSR primer pairs to amplify specific loci was evaluated with genomic DNA of ‘Appalachian Spring’ and
‘Cherokee Brave’. Of these primers, 311 successfully amplified product(s) with ‘Cherokee Brave’ DNA, 21 produced weak or faint
products, and 19 did not amplify any products. Additionally, 218 of the 311 primers pairs revealed polymorphisms between the
two cultivars, and 20 out of 218 primers detected an average of 13.7 alleles from 38 selected Cornus species and hybrids. These SSR loci constitute a valuable resource of ideal markers for both genetic linkage mapping and
gene tagging of flowering dogwood.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
20.
T. Komatsuda S. Kawasaki I. Nakamura F. Takaiwa F. Taguchi-Shiobara S. Oka 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(4):637-642
Recombinant backcross lines of barley were produced from a cross between Kanto Nakate Gold (KNG; two-rowed) and Azumamugi
(AZ; six-rowed) after backcrosses of F1 plants with AZ as the recurrent parent. Each of these lines had an introgressed segment from chromosome 2 of KNG. Two recombinant
backcross lines, L1 and M3-13, were used for an initial screening of polymorphism. After screening a total of 888 oligonucleotides
as arbitrary primers, we identified eight random amplified polymorphic DNAs (RAPDs) between backcross lines and AZ. Among
the RAPD fragments, CMNA-38700 was linked to the v locus with a recombination frequency of zero, while OPJ-09850 and OPP-02700 were linked to the v locus at a map distance of 1.4 cM. Thus, the three RAPD markers were clustered around the v locus since the lengths of introgressed chromosomal segments in the L1 and M3-13 lines were no less than 38 cM. The other
five RAPD fragments that we identified were not linked to the v locus.
Received: 14 January 1997 / Accepted: 14 February 1997 相似文献