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Histone-like nucleoid structuring protein (H-NS) is a modular protein that is associated with the bacterial nucleoid. We used chromatin immunoprecipitation to determine the binding sites of H-NS and RNA polymerase on the Salmonella enterica serovar Typhimurium chromosome. We found that H-NS does not bind to actively transcribed genes and does not co-localize with RNA polymerase. This shows that H-NS principally silences gene expression by restricting the access of RNA polymerase to the DNA. H-NS had previously been shown to preferentially bind to curved DNA in vitro. In fact, at the genomic level we discovered that the level of H-NS binding correlates better with the AT-content of DNA. This is likely to have evolutionary consequences because we show that H-NS binds to many Salmonella genes acquired by lateral gene transfer, and functions as a gene silencer. The removal of H-NS from the cell causes un-controlled expression of several Salmonella pathogenicity islands, and we demonstrate that this has deleterious consequences for bacterial fitness. Our discovery of this novel role for H-NS may have implications for the acquisition of foreign genes by enteric bacteria. 相似文献
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Turning lambda Cro into a transcriptional activator 总被引:9,自引:0,他引:9
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Transcription activation at Class I CAP-dependent promoters 总被引:27,自引:4,他引:23
Richard H. Ebright 《Molecular microbiology》1993,8(5):797-802
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Ricksen S. Winardhi Ranjit Gulvady Jay L. Mellies Jie Yan 《The Journal of biological chemistry》2014,289(20):13739-13750