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1.
The specific activities of pyruvate kinase of cardiac and skeletal (gastrocnemius) muscles of adult rats of both sexes are lower than those of immature rats. The activity does not change after adulthood in the cardiac muscle, but decreases in the gastrocnemius. The activity of pyruvate kinase of the heart of immature and adult rats of both sexes decreases after castration, but is unaffected in old rats. Castration has no effect on the activity of pyrovate kinase of the gastrocnemius muscle of rats of both sexes at any age. In invo administration of estradiol (50 μg/100 g body weight) increases the activity of pyruvate kinase of the heart of castrated male and female rats of the three ages. For the skeletal muscle, the activity increases in castrated adult female and old male rats only. A higher dose (100 μg) of estradiol has variable effects on pyruvate kinase of the heart of male and female castrated rats of different ages. This dose increase pyruvate kinase significantly in the skeletal muscle of old castrated male and female rats. However, it decreases it in the skeletal muscle of adult castrated male rats. Testosterone (100 μm) increases the activity of pyruvate kinase of the heart of castrated male rats. This increase is lower in old age. It has no effect in the heart of castrated female rats of any age. Testosterone (50 μg) increases pyruvate kinase activity of the skeletal muscle of young ovariectomized rats only. A higher dose (100 μg) causes a significant increase in pyruvate kinase of the skeletal muscle of castrated adult and old male, and young and adult female rats, respectively. These data show that sex steroid hormones induce pyruvate kinase of striated muscles, and that the age- and sex-dependent variations may be due to changes in the levels of receptor proteins. 相似文献
2.
The specific activity of creatine phosphokinase (CPK) was measured in the muscle of mdg/mdg and control embryos of 14-18 days' gestation. CPK specific activity values were similar in mutant and normal embryos at the earliest stages examined (14-15 days). However, after 15 1/2 days, the specific activity of the enzyme in the mdg/mdg embryos was approximately 50% lower than in the controls. The dysgenic and normal muscle extracts exhibited comparable stability after storage at -85 C. CPK activity levels in the muscle of adult heterozygotes (+/mdg) and wild-type (+/+) controls were found to be statistically identical. The findings suggest that the mdg mutation does not have a primary or direct effect on CPK activity. 相似文献
3.
The induction of the enzyme creatine phosphokinase (CPK) in cultures of chick breast muscle myoblasts has been distinguished from the process of fusion of myoblasts resulting in the formation of multinucleated myotubes. Primary cultures of myoblasts grown in the presence of phospholipase C, BUdR or EGTA, all of which prevent cell fusion, contain amounts of CPK similar to the level in untreated cultures. Both the brain and muscle isozymes are present in all cultures. We conclude that the induction of CPK is not dependent upon the formation of multinucleated myotubes. 相似文献
4.
R I Potapenko 《Ukrainski? biokhimicheski? zhurnal》1983,55(5):563-566
Adenosine phosphate and creatine phosphate amount was determined in the brain tissue of 3-4-week, 6-8 month and 26-26 month old mongrel female rats. The maximum ATP and creatine phosphate amount and the minimum of ADP and AMP were found in young rats. In adult rats as compared with the young the ATP amount is the same, the ADP and AMP level rises, that of creative phosphate falls and energy charge decreases. In the brain of old rats the ATP and ADP amount falls, that of creatine phosphate and AMP remains at the level of mature-age animals. Despite a decrease in the ATP amount in the brain at the old age, the Mg, DNP- ATPase activity of mitochondria isolated from brain cortex and stem of the old rats remains at the level typical of adult animals. 相似文献
5.
6.
The activity and induction pattern of phosphoenolpyruvate carboxykinase (PEPCK) in the liver and brain of young (6-), adult (30-) and old (90-weeks) male rats were studied. The activity of this enzyme increases in both tissues until adulthood and decreases gradually thereafter. Further, the activity of PEPCK is higher in the liver than the brain. Adrenalectomy decreases significantly the activity of this enzyme in the liver of rats of all ages. However, this treatment inhibits brain PEPCK in young and adult rats. Administration of hydrocortisone to adrenalectomized rats increases PEPCK in both tissues of young and adult rats. However, the magnitude of induction is higher in the young, as compared to the adult, rats. This hormone-mediated induction of the enzyme is actinomycin D-sensitive. 相似文献
7.
The involvement of soluble growth-promoting factors in stretch-induced hypertrophy of the Patagialis muscle (PAT) in the chicken wing was investigated. Soluble extracts were prepared from young chicken PAT muscles made hypertrophic by passive stretch and from unstretched contralateral controls. Extracts were tested for their ability to stimulate cell proliferation and creatine phosphokinase (CPK) activity in primary monolayer cultures of chick embryo muscle cells. Factors were present in muscle extracts which showed a dose-dependent stimulation of cell proliferation and CPK activity in vitro. Passive stretch for 5 days produced a rapid hypertrophy of the PAT which was accompanied by a dramatic increase in the activity of the growth factor(s). Release of stretch resulted in an arrest of growth and an immediate fall in growth factor activity. The difference in growth-stimulating activity between control and stretched PAT extracts could be demonstrated in chicken transferrin-sensitive chick myoblast cultures. Stretch thus induces an increase in a class-specific growth factor, possibly Transferrin, in the PAT. Stretched PAT extracts stimulated: (a) chick myoblast proliferation to a greater extent than an optimum concentration of chick embryo extract, and (b) CPK activity in vitro to a greater extent than excess Transferrin. Both control and stretched PAT extracts supported the growth of rat myoblasts. We conclude that PAT muscle extracts also contain unknown growth factor(s) which are different from Transferrin. 相似文献
8.
Vikrant Vijay Tao Han Carrie L. Moland Joshua C. Kwekel James C. Fuscoe Varsha G. Desai 《PloS one》2015,10(1)
Cardiovascular disease (CVD) is the leading cause of mortality worldwide. Moreover, sex and age are considered major risk factors in the development of CVDs. Mitochondria are vital for normal cardiac function, and regulation of mitochondrial structure and function may impact susceptibility to CVD. To identify potential role of mitochondria in sex-related differences in susceptibility to CVD, we analyzed the basal expression levels of mitochondria-related genes in the hearts of male and female rats. Whole genome expression profiling was performed in the hearts of young (8-week), adult (21-week), and old (78-week) male and female Fischer 344 rats and the expression of 670 unique genes related to various mitochondrial functions was analyzed. A significant (p<0.05) sexual dimorphism in expression levels of 46, 114, and 41 genes was observed in young, adult and old rats, respectively. Gene Ontology analysis revealed the influence of sex on various biological pathways related to cardiac energy metabolism at different ages. The expression of genes involved in fatty acid metabolism was significantly different between the sexes in young and adult rat hearts. Adult male rats also showed higher expression of genes associated with the pyruvate dehydrogenase complex compared to females. In young and adult hearts, sexual dimorphism was not noted in genes encoding oxidative phosphorylation. In old rats, however, a majority of genes involved in oxidative phosphorylation had higher expression in females compared to males. Such basal differences between the sexes in cardiac expression of genes associated with energy metabolism may indicate a likely involvement of mitochondria in susceptibility to CVDs. In addition, female rats showed lower expression levels of apoptotic genes in hearts compared to males at all ages, which may have implications for better preservation of cardiac mass in females than in males. 相似文献
9.
Experiments were conducted to test the hypothesis that injury to skeletal muscle in rats resulting from prolonged downhill running is prevented to a greater extent by prior downhill training than by either uphill or level training. Changes in plasma creatine phosphokinase (CPK) activity and glucose-6-phosphate dehydrogenase (G-6-PDase) activity in the soleus (S), vastus intermedius (VI), and medial head of triceps brachii (TM) muscles were evaluated as markers of muscle injury 48 h after 90 min of intermittent downhill running (16 m . min -1). Prior to this acute downhill run, groups of rats were trained by either downhill (-16 degrees), level (0 degrees), or uphill (+16 degrees) running (16 m . min -1) for 30 min/day. Training duration was either 5 days or 1 day. A training effect (i.e., reduced muscle injury) was indicated if muscle G-6-PDase or plasma CPK activity in a trained group following the 90-min downhill run was not different from that of nonexercised control animals and/or if it was lower than that of nontrained runners. A significant training effect was achieved in all three muscles with 5 days of either downhill or level training, but only in S after 5 days of uphill training. Elevation of plasma CPK activity was prevented by 5 days of training on all three inclines.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
10.
V A Saks N V Lipina G B Chernousova V G Sharov V N Smirnov E I Chazov R Grosse 《Biokhimii?a (Moscow, Russia)》1976,41(12):2099-2109
The functional role of particulate MM isozyme of creatine phosphokinase (CPK) bound to heart myofibrils has been studied. It has been shown that in the presence of heart myofibrils and MgATP creatine phosphate can be used to rephosphorylate ADP formed in the MgATPase reaction. The rate of creatine phosphate splitting is determined by the kinetic properties of myofibrillar MgATPase and by the kinetic parameters of myofibrillar CPK. It has been found that a purified heart plasma membrane preparation contains high CPK activity. CPK isozyme bound to plasma membrane of heart cells is identical to MM isozyme of CPK and is able to rephosphorylate effectively ADP, formed in the (Na K)ATPase reaction. The rate of creatine phosphate splitting in these coupled reactions is sensitive to ouabain and is determined by the kinetic parameters both of the (Na, K)ATPase and plasma membrane CPK. The results obtained indicate the important role of myofibrillar and plasma membrane CPK in the intracellular energy transport processes. 相似文献
11.
D O Levitski? T S Levchenko V A Saks V G Sharov V N Smirnov 《Biokhimii?a (Moscow, Russia)》1977,42(10):1766-1773
The functional role of creatine phosphokinase (CPK) in the process of energy supply for the Ca2+-ATPase reaction and ion transport across the membrane of heart sarcoplasmic reticulum (SR) has been studied. It has been shown that isolated and purified preparations of heart SR contain significant activity of CPK. The localization of CPK on the membrane of SR has been revealed also by an electron microscopic histochemical method. Under conditions of the Ca+-ATPase reaction in the presence of creatine phosphate the release of creatine into the reaction medium is observed, the rate of the latter process being dependent upon the MgATP concentration in accordance with the kinetic parameters of the Ca2+-ATPase reaction. CPK localized on the SR membrane is able to maintain higher rate of calcium uptake by SR vesicles, as compared to that with added ATP-regenerating system. The results obtained demonstrate the close functional coupling between CPK and Ca2+-ATPase in the membrane of SR. 相似文献
12.
With electrophoretic evidence for a mitochondrial isoenzyme of creatine phosphokinase (CPK), we feel that functional studies are necessary to help further elucidate the properties of this isoenzyme. As one approach, fluorodinitrobenzene (FDNB) was used to examine its effect on mitochondrial CPK. In both polarographic studies and direct enzymatic studies, 10?5 M FDNB was shown to almost completely inhibit the enzyme activity, as has been shown in skeletal muscle. In addition it was observed that the mitochondrial CPK was just as susceptible to the inhibitory effect of FDNB as the cytoplasmic isoenzyme. 相似文献
13.
R T Dowell 《Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)》1985,178(4):565-574
Progressive postbirth development of mammalian heart contractile function is accompanied by augmentations of aerobic metabolic potential and cardiac myofibrillar ATPase activity. The temporal similarity of the above developmental sequences suggested that a single, unifying factor may coordinate myocardial maturation. It was hypothesized that cardiac sympathetic nervous system development might be regulating other aspects of myocardial growth. To test this hypothesis, previously well-defined aspects of heart metabolism and contractile protein ATPase activity were determined in rats which were either sympathectomized with 6-hydroxydopamine (6-OHDA) or subjected to chronic, beta-adrenergic blockade (propranolol) throughout the postbirth period from 3 to 6 weeks of age. Neither 6-OHDA treatment nor chronic, beta-adrenergic blockade resulted in a significant reduction of any metabolic enzyme specific activity or in myofibrillar ATPase. Myofibrillar creatine phosphokinase (CPK) activity underwent greater enhancement relative to ATPase during normal heart growth. Significant and divergent influences were exerted by 6-OHDA and propranolol drug regimens on myofibrillar CPK/ATPase enzyme activity ratio. These results indicate (a) the potential for independent regulation of myofibrillar CPK and ATPase, and (b) the advisability of evaluating CPK, ATPase, and CPK/ATPase enzymatic activities as myofibrillar correlates of heart contractile function. Nevertheless, the majority of developmentally related processes in the heart are minimally influenced by chemical sympathectomy. 相似文献
14.
Serum and organ creatine phosphokinase alterations in exercise. 总被引:1,自引:0,他引:1
Rats that swam for 3 h showed a 6-fold increase in serum creatine phosphokinase (SCPK) activity which declined to control values within 7 h after swimming. Of the excess SCPK, 77% was BB isoenzyme; the remainder was mainly MM with traces of MB. Kidney, liver, brain and lung contain mainly BB (50-80%) and only a trace of MB (0-7%). Heart CPK was composed of little BB (8%) and more MB (28%) and MM (64%). Skeletal muscle CPK was almost entirely MM. CPK activity is highest in skeletal muscle, intermediate in heart and brain and lowest in kidney, liver and lung. It is suggested that skeletal muscle and heart are not involved in CPK release in swimming, and kidney, liver and brain may be sites of release. 相似文献
15.
Activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), creatine phosphokinase (CPK), and lactate dehydrogenase (LDH) were determined in plasma, kidney, liver, and muscle from five species of captive birds. Few differences occurred in plasma activities between sexes but considerable differences occurred between species. All five enzymes were detected in each of the tissues sampled. Relative enzyme activities in liver, kidney, and muscle were similar for each species. CPK activity was much higher in muscle than in liver or kidney and, of the five enzymes studied, may be the best indicator of muscle damage. Most of the other enzymes were more evenly distributed among the three tissues, and no organ-specific enzyme could be identified for liver or kidney. Because of interspecific variations in plasma enzyme activities, it is important to establish baseline values for each species to ensure accurate interpretation of results. 相似文献
16.
The standard oxygen consumption rate and the activities of muscle citrate synthase, creatine phosphokinase and lactate dehydrogenase in the tropical fish Oreochromis niloticus acclimated to either 20.5 ± 0.3° C or 26.5 ± 0 ± 5 ± C for at least 3 months were investigated. The standard oxygen consumption rate of individual fish from the two acclimation temperatures was determined at 20, 25 and 30 ± C. At all experimental temperatures, the standard oxygen consumption rate of fish acclimated to 20.5 ± 0.3° C was significantly higher than that of fish kept at 26.5 ± 0.5 ± C. In both groups smaller individuals had a higher oxygen consumption rate than large ones.
Analyses of the activity levels of citrate synthase (CS), creatine phosphokinase (CPK) and lactate dehydrogenase (LDH) in both red and white muscles isolated from fish kept under the two temperature regimes were performed at 26 ± C. The activity of CS in both red and white muscles isolated from the 20.5 ± 0.3° C acclimated fish was significantly higher than that of muscles isolated from the 26.5 ± 0.5 ± C acclimation group. Similarly, the CPK activity in white muscles isolated from fish acclimated to 20.5 ± 0.3 ± C was higher than that of muscles obtained from the 26.5 ± 0.5 ± C acclimation group. However, the CPK activity in red muscles isolated from the two fish groups was not significantly different. The opposite results were obtained for LDH activity. For example, the LDH activity of white muscles isolated from fish acclimated to 26.5 ± 0.5 ± C was significantly higher than that of the same muscles but from the 20.5 ± 0.3 ± C acclimated fish. No differences were observed in the LDH activity of red muscles isolated from the two fish groups. 相似文献
Analyses of the activity levels of citrate synthase (CS), creatine phosphokinase (CPK) and lactate dehydrogenase (LDH) in both red and white muscles isolated from fish kept under the two temperature regimes were performed at 26 ± C. The activity of CS in both red and white muscles isolated from the 20.5 ± 0.3° C acclimated fish was significantly higher than that of muscles isolated from the 26.5 ± 0.5 ± C acclimation group. Similarly, the CPK activity in white muscles isolated from fish acclimated to 20.5 ± 0.3 ± C was higher than that of muscles obtained from the 26.5 ± 0.5 ± C acclimation group. However, the CPK activity in red muscles isolated from the two fish groups was not significantly different. The opposite results were obtained for LDH activity. For example, the LDH activity of white muscles isolated from fish acclimated to 26.5 ± 0.5 ± C was significantly higher than that of the same muscles but from the 20.5 ± 0.3 ± C acclimated fish. No differences were observed in the LDH activity of red muscles isolated from the two fish groups. 相似文献
17.
A new colorimetric method has been developed for the determination of creatine phosphokinase (CPK). This method is based on the reaction of creatine, formed enzymatically from creatine phosphate and ADP, with p-nitrophenylglyoxal (PNPG) under alkaline conditions to produce a colored product which absorbs maximally at 480 nm. At 25 degrees C the reaction was complete after 10 min in 0.1 M sodium pyrophosphate, pH 12, containing 0.15 M sodium ascorbate. The colored product was stable for at least 24 h and obeyed Beer's law in the range 0.005-0.05 mM creatine. The color reaction was used to determine the activity of CPK in serum and tissue extracts. The results obtained by this method agreed well with the results obtained by other available methods for CPK determination. However, the PNPG method was more rapid and more sensitive than other colorimetric methods and required a single chromogenic reagent. 相似文献
18.
Vicente Andrés Roser Cussó José Carreras 《Differentiation; research in biological diversity》1989,41(1):72-77
Phosphoglycerate mutase and creatine phosphokinase have in mammals three isozymes (types MM, MB and BB) with similar tissue distribution and developmental transition in muscle cells. To assess whether the phenotype and the developmental switch of these isozymes differ in the diverse types of muscle fibers, the enzymatic activities and the isozyme patterns, analyzed by cellulose acetate electrophoresis, have been determined in rat soleus, extensor digitorum longus and gastrocnemius muscles during postnatal development. Both phosphoglycerate mutase and creatine phosphokinase activity increased in the three muscles, the increase in extensor digitorum longus and gastrocnemius being higher than in soleus. For the two enzymes the increase in activity was due to the progressive increment of the muscle-specific forms. It is concluded that whereas phosphoglycerate mutase and creatine phosphokinase type-B subunits are present at similar levels in both type I and type II muscle fibers, phosphoglycerate mutase and creatine phosphokinase type-M subunits exhibit much higher levels in type II fibers. 相似文献
19.
Since little is known about the training response to exercise in neonatal animals, this study was undertaken to elucidate the potential of oxidative system adaptations in developing skeletal muscle of rats during 50 days of daily treadmill running. The training regimen involved male and female rats (10 days old) initially running 0.1 mph, 0% grade, for 15 min. The program progressed to 1 mph, 25% grade, for 60 min by 50 days of age. At 25 days of age, pyruvate and palmitate oxidative capacity, and citrate synthase activity in red vastus muscle homogenates were elevated in the trained group (T) compared with age- and sex-matched controls (C). These increases were also observed for each subsequent time point tested and occurred in spite of the fact that the peak oxidative capacity of neonatal red vastus muscle was 46% greater than adult values. Further, trained animals tested at 45 days of age responded with a 12% increase in maximal oxygen consumption (Vo2max) compared with controls (P less than 0.05). Assays of muscle phosphofructokinase and of creatine phosphokinase activity conducted at this time point revealed no difference between T and C groups. Collectively, these data suggest that neonatal rats can be successfully trained and that they respond to an endurance-type program qualitatively similarly to adult rats. 相似文献
20.
Control by fibroblast growth factor of differentiation in the BC3H1 muscle cell line 总被引:36,自引:18,他引:18
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The regulation of creatine phosphokinase (CPK) expression by polypeptide growth factors has been examined in the clonal mouse muscle BC3H1 cell line. After arrest of cell growth by exposure to low concentrations of serum, BC3H1 cells accumulate high levels of muscle-specific proteins including CPK. The induction of this enzyme is reversible in the presence of high concentrations of fetal calf serum, which cause quiescent, differentiated cells to reenter the cell cycle. Under these conditions, the rate of CPK synthesis is drastically reduced. We show in the present communication that either pituitary-derived fibroblast growth factor (FGF) or brain-derived FGF are as effective as serum in repressing the synthesis of CPK when added to quiescent, differentiated cells. The decrease in the rate of synthesis of CPK occurs within 22 h after the addition of pituitary FGF to the cells. Pituitary FGF had very little effect, if any, on the rate CPK degradation. The overall rate of protein synthesis and the pattern of synthesis of the major polypeptides made by these cells was not altered by the addition of FGF. Although pituitary FGF was mitogenic for BC3H1 cells, the rate of cell growth was not absolutely correlated with the extent of repression of CPK. Brain-derived FGF fully repressed CPK induction under conditions where it showed no significant mitogenic activity. These results show that the expression of a muscle-specific protein, CPK, can be controlled by a single defined polypeptide growth factor in fully differentiated cultures, and that initiation of cell division is not required for their regulation to take place. 相似文献