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目的:建立高效液相色谱同时测定引种白芷中5种香豆素成分含量的方法,为筛选出适合江苏栽培的白芷种源提供依据.方法:采用Phenomenex Gemini C18(250 mm×4.60 mm,5μm)色谱柱;流动相为甲醇-水(70∶30),流速0.8 mL·min-1,柱温30℃;UV检测波长310 nm.结果:江苏引种的不同种质来源白芷中香豆素的含量差异明显.结论:该检测方法简单,准确,重现性好.江苏引种的川白芷生长量最大,且五种香豆素的总含量最高. 相似文献
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采用高效液相色谱法同时测定明党参组培和栽培品中7-羟基香豆素、花椒毒酚、欧前胡素和珊瑚菜内酯4种香豆素成分的含量。Agilent-C18色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈—水溶液梯度洗脱,流速1.0 mL/min,柱温30℃;检测波长为314 nm,进样量为10μL。实验结果表明,栽培明党参的根皮4种香豆素成分7-羟基香豆素、花椒毒酚、欧前胡素、珊瑚菜内酯的含量普遍偏高,分别为5.8970、1.2710、0.0301、0.9236mg/g,明党参愈伤组织诱导根中7-羟基香豆素、花椒毒酚含量较高分别为3.7939、0.8384 mg/g;该方法简便、快速、具有良好的重现性,可用于明党参组培和栽培品中香豆素成分的含量测定。 相似文献
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建立HPLC法同时测定不同产地川佛手中木犀草素、6,7-二甲氧基香豆素、莨菪亭、5,7-二甲氧基香豆素、7-羟基香豆素、橙皮苷、槲皮素、阿魏酸8种成分含量的方法。采用Thermo BDS HYPERSIL C18(250 mm×4. 6 mm,5μm)色谱柱,甲醇-0. 2%冰醋酸水溶液进行梯度洗脱,流速1 m L/min,柱温30℃,检测波长322 nm。在该色谱条件下,川佛手中8种成分分离良好,平均加样回收率为91. 22%~96. 52%,RSD <5. 0%。不同产地的川佛手中8种成分含量存在差异,部分成分之间存在相关性,样品聚为3类。产于四川省宜宾市长宁县老翁镇的川佛手质量最优。该方法可为川佛手的资源开发利用、质量控制以及安全评价提供参考依据。 相似文献
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《中国野生植物资源》2019,(3)
目的:比较新疆戈宝麻花、种子、叶片中芦丁的含量。方法:采用回流提取法提取芦丁,高效液相层析法法测定新疆戈宝麻不同部位中芦丁的含量。色谱柱为Eclipse-C18柱(4.6 mm×150 mm, 5 um);流动相为乙腈-0.25%磷酸溶液(V/V,14.5∶85.5);流速为1.0 mL/min;柱温为30℃;检测波长为360 nm;进样量为20 uL。结果:芦丁含量在0.01~0.1 mg/mL范围内与峰面积呈良好的线性关系,相关系数为0.993 6;其中花、种子、叶片中芦丁得率分别为:0.094%、0.014%、0.6%。结论:新疆戈宝麻不同部位芦丁的含量分布为:叶﹥花﹥种子,上述结果可为增加戈宝麻新药源和新的药用部位开发利用提供有力依据。 相似文献
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采用高效液相色谱法建立了白芷水提液指纹图谱,色谱柱为Lichrospher 5-C18 (250 mm×4.6 mm,5μm),以甲醇-水为流动相进行二元梯度洗脱,柱温30℃,流速为1 mL/min,检测波长为254 nm.对12批不同产地的白芷药材进行了检测,共发现13个共有色谱峰,各色谱峰分离情况良好.精密度、重复性和稳定性试验中各共有峰相对保留时间和相对峰面积的RSD均小于3%,符合指纹图谱要求.该方法简便、可靠,精密度、稳定性和重复性良好,可用于白芷药材的质量评价. 相似文献
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林进生 《中国野生植物资源》2022,(1):44-46
目的:建立蛇床子中蛇床子素的含量测定方法.方法:采用高效液相色谱法,色谱柱选择Agilent ZORBAX SB-C18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-体积分数0.05%磷酸溶液(体积比70:30),流速为1.0 mL/min,检测波长为322 nm,柱温30℃.结果:蛇床子素的线性关系方程... 相似文献
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建立新疆阿魏中不同部位阿魏酸的高效液相色谱含量测定方法.采用PLATISIL ODS(250 mm×4.6mm,5μm)色谱柱,甲醇~0.1%冰醋酸水溶液(35∶ 65)为流动相,流速1.0 mL/min,检测波长320 nm,柱温30℃.测得阿魏酸进样量在0.440~5.396μg/mL(r=0.9997)范围内与峰面积有良好的线性关系,平均回收率达97.8%,RSD为1.79%(n=9).新疆阿魏胶、根、茎和叶中的阿魏酸含量分别为0.2600、0.0362、0.0225和0.0275 mg/g,胶中阿魏酸的含量远高于根、茎和叶.该方法简便、准确、重复性好,可用于新疆阿魏中不同部位阿魏酸含量的测定. 相似文献
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目的:考察不同采收期东北铁线莲药材中灵仙新苷(Clematichinenoside AR)含量,研究其动态含量变化和最佳采收期.方法:采用色谱柱Diamonsil C18(250 mm×4.6 mm,5μm)柱,流动相:乙腈-水溶液梯度洗脱程序,流速1.0 mL/min,检测波长203 nm,柱温:30℃.结果:灵仙新苷以8月下旬到9月中旬的含量最高.结论:东北铁线莲药材的最佳采收期为8月下旬到9月中旬. 相似文献
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Giuseppe Benedetti 《Mycopathologia》1943,3(3-4):262-279
Riassunto L'A. ha isolato un ceppo di Mycocandida pseudotropicalis da un caso di broncopneumomicosi pura e primitiva in una giovane donna. Vengono illustrate le caratteristiche colturali, morfologiche e biochimiche del ceppo e sono riferite le ricerche immunologiche, cliniche e sperimentali anatomo-patologiche. Una indagine particolare è rivolta allo studio delle assimilazioni, in special modo dei carboidrati (glicogene, amido, glucosio). Dopo aver illustrato la storia della specie, l'A. ne discute la validità nomenclatoriale, la posizione sistematica in seno alle classificazioni dei lieviti anascosporei.
Zusammenfassung Der Autor hat einen Stamm der Mycocandida pseudotropicalis aus einem Fall reiner und primitiver Bronchopneumomykose bei einer jungen Frau isoliert. Es werden nun die kultivatorischen, morphologischen und biochemischen Charakteristica des Stammes erläutert und es wird über die immunologischen, klinischen und anatomisch-pathologisch experimentellen Forschungen berichtet. Ein besonderes Augenmerk ist auf das Studium der Assimilationen, vor allem der Carbohydrate (Glykogen, Stärke, Glykose) gerichtet. Nach einer Erläuterung der Artgeschichte diskutiert der Autor über die Gültigkeit der Nomenklatur und über die systematische Stellung zwischen den Klassifikationen der anascosporogenen Hefen.相似文献
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Tun-Hai Xu Ya-Juan Xu Dong Han Hong-Feng Zhao Sheng-Xu Xie Dong-Ming Xu 《植物学报(英文版)》2007,49(2):202-206
To investigate saponins from the roots of Pulsatilla cernua (Thunb.) Bercht. et Opiz., two new compounds together with five known trlterpenold saponins were isolated. The structures of the two new trlterpenoid saponins, named cernuasides A and B, were elucidated as 3-O-[β-D-xylopyranosyl(1-)2)]-[α-L-rhamnopyranosyl(1-)4)]-α-L- arablnopyranosyl hederagenin 28-O-β-D-glucopyranosyl ester (compound 1) and 3-O-[α-L-arabinopyranosyl(1→)3)]- [α-L-rhamnopyranosyl (1→)2)]-α-L-arabinopyranosyl hederagenin 28-O-β-D-glucopyranosyl ester (compound 2) by 1D, 2D-NMR techniques, ESIMS analysis, as well as chemical methods. 相似文献
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G. Caretta 《Mycopathologia》1960,12(3):233-250
Riassunto Sono studiati nelle loro caratteristiche morfo-culturali e biologiche quattro ceppi omogenei diCoremiella isolati da feci umane. Questo genera è simile aGeotrichum ma oltre a cellule miceliche clamidosporo-simili presenta formazione apicali miceliche di tipo coremioide reptante.L'artrosporulazione è un processo endogeno come inGeotrichum, e come inGeorichum si hanno artrospore clamidosporioidi fertili opache e artrospore usuali non fertili e jaline, ma meno differenziate.I ceppi vengono riferiti aC. ulmariae e le caratteristiche vengono paragonate anche con le culture su legno diSpirea ulmaria.Il primo esemplare diCoremiella è stato pubblicato comeOospora cuboidea
Sacc. etEll. come è stato accertato studiando l'esemplare autentico diEllis.In conclusione il genereCoremiella
Bub. etKrieg. è stato emendato ed è stata creata la combinazioneC. cuboidea (Sacc. etEll.).
Summary Four homogeneous strains ofCoremiella from human feces were studied from morpho-cultural and biological standpoint. This genus is likeGeotrichum, but in addition to some mycelial, chlamydospore-like cells, coremioid mycelial, reptant strands are formed.The arthrosporulation is an endogeneous process, and, likeGeotrichum, there are usual hyaline and duller chlamydosporioid, fertile arthrospores, but less differentiated.The strains are referred toC. ulmariae, and the characteristics were compared also on timber and woody European Meadowsweet cultures. But the first binomial ofCoremiella has been published asOospora cuboidea Ell. etSacc., as ascertained by the study of the authenticEllis specimen.In conclusion, the genusCoremiella Bub. etKrieg. has been emended, and the combinationC. cuboidea (Sacc. etEll.) established.相似文献
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To investigate saponins from the roots of Pulsatilla cernua (Thunb.) Bercht. et Opiz., two new compounds together with five known triterpenoid saponins were isolated. The structures of the two new triterpenoid saponins, named cernuasides A and B, were elucidated as 3-O-[β-D-xylopyranosyl(1→2)]-[α-L-rhamnopyranosyl(1→4)]-α-Larabinopyranosyl hederagenin 28-O-β-D-glucopyranosyl ester (compound 1) and 3-O-[α-L-arabinopyranosyl(1→3)]-[α-L-rhamnopyranosyl (1→2)]-α-L-arabinopyranosyl hederagenin 28-O-β-D-glucopyranosyl ester (compound 2) by 1D, 2D-NMR techniques, ESIMS analysis, as well as chemical methods. 相似文献
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A. E. Szmidt X. -R. Wang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1993,86(2-3):159-165
Summary Allozyme variation was examined in 22 populations of Pinus densiflora (Sieb, et Zucc.) and four geographic varieties of P. sylvestris (L.): var lapponica (Fries, Hartman), var armena (Komarov), var mongolica (Litvinov) and var sylvestriformis (Takenouchi). In addition, we developed paternal chloroplast (cp) DNA markers that distinguish P. densiflora from var lapponica, var armena and var mongolica. UPGMA cluster analysis based on Nei's distances between all pairwise combinations of the 22 populations revealed patterns corresponding strictly to geographic origin and taxonomic status. Analysis of allozyme variation in var lapponica, var armena and var mongolica demonstrated a high level of intrapopulational variability but a low level of interpopulational differentiation. It appears that the late Pleistocene blending of genetically diverse populations was responsible for the observed variation patterns. The constructed phylogenetic trees also showed late divergence of these three varieties. The var sylvestri formis was genetically distinct from the other three P. sylvestris varieties. The genetic distances separating var sylvestriformis from P. densiflora and the other taxa lend support to a separate taxonomic status for var sylvestriformis and a close relation with P. densiflora. We found that var sylvestriformis harbors admixtures of allozymes and cpDNA from both P. sylvestris and P. densiflora, which suggests an introgressive nature of this variety. Levels of intrapopulational variability were similar in P. sylvestris and P. densiflora, but interpopulational differentiation was much higher in P. densiflora. In the constructed phylogenetic trees, populations of this species were characterized by relatively long internode distances and branch lengths. The present results suggest that P. densiflora has a more advanced evolutionary age than P. sylvestris. 相似文献