共查询到20条相似文献,搜索用时 15 毫秒
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IncorporationofResistanceGeneintoTomatousingBinaryVector SystemZHAOShu-hui(赵淑慧);XUEGuo-xiong(薛国雄)(InstituteofDevelopmentalBio... 相似文献
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建立了一种利用双T-DNA载体培育无选择标记转基因植物的方法。通过体外重组构建了双T-DNA双元载体pDLBRBbarm。载体中,选择标记nptⅡ基因和另一代表外源基因的bar基因分别位于2个独立的T-DNA。利用农杆菌介导转化烟草(Nicotiana tabacum L.),在获得的转化植株中,同时整合有nptⅡ基因和bar基因的频率为59.2%。对4个同时整合有nptⅡ和bar基因植株自交获得的T1代株系进行检测分析,发现在3个T1代株系2个T-DNA可以发生分离,其中约19.5%的转基因T1代植株中只存在bar基因而不带选择标记nptⅡ。这一结果说明双T-DNA载体系统能有效地用于培育无选择标记的转基因植物。研究还利用位于2个不同载体上的nptⅡ基因与bar基因通过农杆菌介导共转化烟草,获得共转化植株的频率为20.0%~47.4%,低于使用双T-DNA转化的共转化频率。 相似文献
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建立了一种利用双T-DNA载体培育无选择标记转基因植物的方法.通过体外重组构建了双T-DNA双元载体pDLBRBbarm.载体中,选择标记nptⅡ基因和另一代表外源基因的bar基因分别位于2个独立的T-DNA.利用农杆菌介导转化烟草(Nicotiana tabacum L.),在获得的转化植株中,同时整合有nptⅡ基因和bar基因的频率为59.2%.对4个同时整合有nptⅡ和bar基因植株自交获得的T1代株系进行检测分析,发现在3个T1代株系2个T-DNA可以发生分离,其中约19.5%的转基因T1代植株中只存在bar基因而不带选择标记nptⅡ.这一结果说明双T-DNA载体系统能有效地用于培育无选择标记的转基因植物.研究还利用位于2个不同载体上的nptⅡ基因与 bar基因通过农杆菌介导共转化烟草,获得共转化植株的频率为20.0%~47.4%,低于使用双T-DNA转化的共转化频率. 相似文献
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小麦耐逆基因-TaLEA2转化拟南芥的研究 总被引:9,自引:0,他引:9
研究小麦第3组LEA基因中T aLEA2对耐旱和耐盐性能的影响.将小麦第3组LEA基因T aLEA2连接在双元表达载体pB I121 C aM V 35S启动子下游,构建了能在植物中高效表达的载体pB I121-T aLEA2.通过农杆菌介导的真空渗透法,将其转入野生拟南芥中,经抗性筛选及PCR验证,获得T0代转基因植株,并用不同浓度的PEG 4000和N aC l对转基因拟南芥的耐逆性进行检测.结果表明,这些转基因植株可明显改进拟南芥在10%PEG及0.8%N aC l培养基上的生长状态.在实验条件下,转基因拟南芥的耐旱性及耐盐性均有所提高,提示T aLEA2基因在植物水分调节方面有重要作用. 相似文献
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《Bioscience, biotechnology, and biochemistry》2013,77(4):804-807
We made two series of Gateway binary vectors, pGWBs and R4pGWBs, possessing a UDP-N-acetylglucosamine: dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene driven by the nopaline synthase promoter (Pnos) as a tunicamycin resistance marker for the transformation of Arabidopsis thaliana. The reporters and tags employed in this system are sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, TagRFP, 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7, and TAP. Selection of transformants was successful on plates containing 0.15 mg/L of tunicamycin. These vectors were compatible with existing pGWB and R4pGWB vectors for kanamycin, hygromycin B, and BASTA® selection, and are useful new tools for making transgenic Arabidopsis. 相似文献
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增加作物的叶绿素含量,尤其是叶绿素b的含量,是增强作物耐弱光性的可能途径.将拟南芥叶绿素合成的关键酶——谷氨酰tRNA还原酶(glutamyl-tRNA reductase,HEMAl)的基因和促进叶绿素b合成的关键酶——叶绿素酸酯a加氧酶(chlorophyllide a oxygenase,CAO)的基因,构建于同一个植物双元表达载体,经农杆菌介导整合进烟草基因组.结果显示,弱光下转基因烟草比野生烟草的叶绿素总量增加不显著,但叶绿素b含量增加16%~17%、叶绿素a/b比值降低9% ~12%、光合作用增强42% ~65%,均达到显著水平,而且生长发育比野生烟草快.为今后改良作物的耐弱光性奠定了基础. 相似文献
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J. Langridge 《BioEssays : news and reviews in molecular, cellular and developmental biology》1994,16(10):775-778
Arabidopsis thaliana is a small cruciferous weed which grows naturally, mainly in Europe. Because of its qualities of small size, rapid growth, low chromosome number and self-fertilisation, I adapted it to aseptic growth in purified agar in sterile test-tubes. I found that it secreted various substances into the medium, but not in type or amount likely to interfere with the expression of biosynthetic mutants. Following X-irradiation of seed, I obtained a number of mutants, including several lethals. One lethal mutant I discovered to be deficient in thiamine synthesis. It was the first biosynthetic mutant to be found in flowering plants. 相似文献
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The Metabolism of cis ABA-aldehyde by the Wilty Mutants of Potato, Pea and Arabidopsis thaliana 总被引:1,自引:0,他引:1
DUCKHAM S. C.; TAYLOR I. B.; LINFORTH R. S. T.; AL-NAIEB R. J.; MARPLES B. A.; BOWMAN W. R. 《Journal of experimental botany》1989,40(8):901-905
RS-[2H1] cis ABA-aldehyde was fed to ABA-deficient mutants ofpotato (droopy), pea (wilty) and Arabidopsis thaliana (aba1)along with appropriate non-mutant controls. Both the wilty andaba1 mutants readily oxidized the monodeuterated ABA-aldehydeto ABA. The incorporation of label into ABA by these two mutantswas indistinguishable from that detected in the non-mutant controls.In contrast, the droopy mutants poorly incorporated the labelledprecursor into ABA. Instead they reduced and isomerized RS-[2H1] cis ABA-aldehyde to a mixture of 2, cis and 2, trans ABA-alcohols.Thus the droopy mutant affects the last step in ABA biosynthesis,a position it shares with the tomato mutants, flacca and sitiens.Genetic evidence suggesting that droopy and sitiens may be correspondinggene loci is discussed. Key words: ABA metabolism, wilty mutants, pea, potato, Arabidopsis 相似文献
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Pajerowska KM Parker JE Gebhardt C 《Molecular plant-microbe interactions : MPMI》2005,18(10):1107-1119
Defense against pests and pathogens is a fundamental process controlled by similar molecular mechanisms in all flowering plants. Using Arabidopsis thaliana as a model, steps of the signal transduction pathways that link pathogen recognition to defense activation have been identified and corresponding genes have been characterized. Defense signaling (DS) genes are functional candidates for controlling natural quantitative variation of resistance to plant pathogens. Nineteen Arabidopsis genes operating in defense signaling cascades were selected. Solanaceae EST (expressed sequence tag) databases were employed to identify the closest homologs of potato (Solanum tuberosum). Sixteen novel DS potato homologs were positioned on the molecular maps. Five DS homologs mapped close to known quantitative resistance loci (QRL) against the oomycete Phytophthora infestans causing late blight and the bacterium Erwinia carotovora subsp. atroseptica causing blackleg of stems and tuber soft rot. The five genes are positional candidates for QRL and are highly sequence related to Arabidopsis genes AtSGT1b, AtPAD4, and AtAOS. Full-length complementary DNA and genomic sequences were obtained for potato genes StSGT1, StPAD4, and StEDS1, the latter being a putative interactor of StPAD4. Our results form the basis for further studies on the contributions of these candidate genes to natural variation of potato disease resistance. 相似文献
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Seo S Gomi K Kaku H Abe H Seto H Nakatsu S Neya M Kobayashi M Nakaho K Ichinose Y Mitsuhara I Ohashi Y 《Plant & cell physiology》2012,53(8):1432-1444
The soil-borne bacterial pathogen Ralstonia solanacearum invades a broad range of plants through their roots, resulting in wilting of the plant, but no effective protection against this disease has been developed. Two bacterial wilt disease-inhibiting compounds were biochemically isolated from tobacco and identified as sclareol and cis-abienol, labdane-type diterpenes. When exogenously applied to their roots, sclareol and cis-abienol inhibited wilt disease in tobacco, tomato and Arabidopsis plants without exhibiting any antibacterial activity. Microarray analysis identified many sclareol-responsive genes in Arabidopsis roots, including genes encoding or with a role in ATP-binding cassette (ABC) transporters, and biosynthesis and signaling of defense-related molecules and mitogen-activated protein kinase (MAPK) cascade components. Inhibition of wilt disease by sclareol was attenuated in Arabidopsis mutants defective in the ABC transporter AtPDR12, the MAPK MPK3, and ethylene and abscisic acid signaling pathways, and also in transgenic tobacco plants with reduced expression of NtPDR1, a tobacco homolog of AtPDR12. These results suggest that multiple host factors are involved in the inhibition of bacterial wilt disease by sclareol-related compounds. 相似文献
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AGPase基因植物表达载体的构建及对烟草的转化 总被引:1,自引:0,他引:1
将大肠杆菌BL21的AGPase基因定向连接在受体质粒pCAMBIA2301载体上,从而构建植物表达载体,转化烟草获得了转基因植株。经GUS组织化学染色、PCR、Southern blot以及RT-PCR鉴定证实,该基因已导入烟草基因组中。淀粉含量测定结果显示,转基因植株比非转基因植株淀粉含量平均增加了13.1%,由此验证了该载体构建的成功与可用性,为下一阶段用该载体转化木薯主栽品种提高块根淀粉含量的研究奠定了基础。 相似文献
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棉花乙烯合成基因促进拟南芥和烟草不定根发生的研究 总被引:1,自引:0,他引:1
从棉花纤维cDNA中克隆获得乙烯合成基因GhACO3,构建了植物过量表达载体p35S::GhACO3.通过花序侵染法和叶盘法分别转化拟南芥和烟草,利用卡那霉素筛选及分子检测获得转基因阳性拟南芥和烟草植株.结果表明,GhACO3基因已整合到拟南芥和烟草基因组中;经过纯合筛选后获得转基因T2代拟南芥植株;与野生型拟南芥相比,GhACO3基因对拟南芥不定根发生具有显著促进作用;与野生型烟草植株相比,转GhACO3基因烟草不定根发生得到了显著的促进.研究表明,GhACO3基因的过量表达能够促进拟南芥和烟草不定根的形成发育,为进一步探讨GhACO3的生物学功能和进行转基因育种奠定了基础. 相似文献
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T. Pélissier S. Tutois J. M. Deragon S. Tourmente S. Genestier G. Picard 《Plant molecular biology》1995,29(3):441-452
An analysis of Arabidopsis thaliana heterochromatic regions allowed the identification of a new family of retroelements called Athila. These 10.5 kb elements, representing ca. 0.3% of the genome, present several features of retrotransposons and retroviruses. Athila elements are flanked by 1.5 kb long terminal repeats (LTR) that are themselves bounded by 5 bp perfect inverted repeats. These LTRs start and end with the retroviral consensus 5TG...CA3 nucleotides. A putative tRNA-binding site and a polypurine tract are found adjacent to the 5 and 3 LTR respectively. The central domain is composed of two long open reading frames (ORFs) of 935 and 694 amino acids. Despite several indications of recent transposition activity, the translation of these ORFs failed to reveal significant homology with proteins associated to retrotransposition. We suggest that the Athila family could result from the transduction and dispersion of a cellular gene by a retrotransposon. 相似文献
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Development of Transformation System of Rice Based on Binary Bacterial Artificial Chromosome (BIBAC) Vector 总被引:1,自引:0,他引:1
Rui-Feng HE 《Acta Genetica Sinica》2006,33(3):269-276