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1.
Adrenal gland function is mediated through secreted hormones, which play a vital role in the autonomic and hypothalamic-pituitary-adrenal (HPA)-axis-mediated stress response. The genetic underpinnings of the stress response can be approached using a quantitative trait locus (QTL) analysis. This method has been used to investigate genomic regions associated with variation in complex phenotypes, but it has not been used to explore the structure of the adrenal. We used QTL analyses to identify candidate genes underlying adrenal weight and adrenal cortical zone and medulla widths. We used 64 BXD recombinant inbred (RI) strains of mice (n?=?528) and 2 parental strains (C57BL/6J and DBA/2J; n?=?20) to measure adrenal weights and adrenal zone widths. For adrenal weight, we found significant QTLs on chromosome 3 for females (Fawq1) and Chr 4 for males (Mawq1) and suggestive QTLs on Chrs 1, 3, 10, and 14 for females and Chrs 2, 4, 10, 17, and X for males. We identified a significant QTL on Chr 10 (Mawdq1) and a suggestive QTL on Chr 13 for male adrenal total width. For male adrenal medulla width, we found a significant QTL on Chr 5 (Mmwdq1) and a suggestive QTL on Chr 1. We also identified significant QTLs on Chrs 10 (Mxwdq1) and 14 (Mxwdq2) for male X-zone width. There are 113 genes that mapped within the significant QTL intervals, and we identified 4 candidate genes associated with adrenal structure and/or function. In summary, this study is an important first step for detecting genetic factors influencing the structure of the adrenal component of the HPA axis using QTL analyses, which may relate to adrenal function and provide further insights into elucidating genes critical for stress-related phenotypes. 相似文献
2.
Eui‐Soo Kim Tad S. Sonstegard Marcos Vinicius G. B. Silva Louis C. Gasbarre Curtis P. Van Tassell 《Animal genetics》2014,45(1):117-121
DNA markers associated with quantitative trait loci (QTL) affecting host tolerance to gastrointestinal (GI) parasite infection are ideal targets for marker‐assisted selection. However, few studies in cattle have attempted to identify this type of QTL due to the difficulty of generating accurate phenotypic data from a resource population with adequate statistical power for detection. For this effort, we amassed fecal egg count (FEC) measures from annual natural field challenges with GI nematodes that spanned 12 different contemporary groups of Angus calves (1992–2000) derived from a closed breeding population. FEC and blood pepsinogen measures were taken weekly over a 26‐week period post‐weaning, and the FEC data were Box‐Cox transformed to normalize the distribution of phenotypes. These 305 test animals and more than 100 founding animals from the extended pedigree were genotyped across 190 microsatellites markers. The genome‐wide analyses identified a suggestive genome‐wide QTL on bovine chromosome (Chr) 8 (P < 0.002) and nominal QTL on Chr 4, 12 and 17 (P < 0.05). These findings were unique for cattle, and some corresponded to previously identified QTL locations for parasite‐related traits in sheep to provide genome locations for further fine mapping of parasite resistance/susceptibility in Angus cattle. 相似文献
3.
Magalie S. Leduc Rachael S. Hageman Qingying Meng Ricardo A. Verdugo Shirng‐Wern Tsaih Gary A. Churchill Beverly Paigen Rong Yuan 《Aging cell》2010,9(5):823-836
The IGF‐1 signaling pathway plays an important role in regulating longevity. To identify the genetic loci and genes that regulate plasma IGF‐1 levels, we intercrossed MRL/MpJ and SM/J, inbred mouse strains that differ in IGF‐1 levels. Quantitative trait loci (QTL) analysis of IGF‐1 levels of these F2 mice detected four QTL on chromosomes (Chrs) 9 (48 Mb), 10 (86 Mb), 15 (18 Mb), and 17 (85 Mb). Haplotype association mapping of IGF‐1 levels in 28 domesticated inbred strains identified three suggestive loci in females on Chrs 2 (13 Mb), 10 (88 Mb), and 17 (28 Mb) and in four males on Chrs 1 (159 Mb), 3 (52 and 58 Mb), and 16 (74 Mb). Except for the QTL on Chr 9 and 16, all loci co‐localized with IGF‐1 QTL previously identified in other mouse crosses. The most significant locus was the QTL on Chr 10, which contains the Igf1 gene and which had a LOD score of 31.8. Haplotype analysis among 28 domesticated inbred strains revealed a major QTL on Chr 10 overlapping with the QTL identified in the F2 mice. This locus showed three major haplotypes; strains with haplotype 1 had significantly lower plasma IGF‐1 and extended longevity (P < 0.05) than strains with haplotype 2 or 3. Bioinformatic analysis, combined with sequencing and expression studies, showed that Igf1 is the most likely QTL gene, but that other genes may also play a role in this strong QTL. 相似文献
4.
A genome-wide scan for quantitative trait loci (QTLs) controlling body weight at 10 weeks after birth was carried out in
a population of 387 intersubspecific backcross mice derived from a cross between C57BL/6J inbred mice (Mus musculus domesticus) and wild mice (M. m. castaneus) captured in the Philippines, in order to discover novel QTLs from the wild mice that have about 60% lower body weight than
C57BL/6J. By interval mapping, we detected four QTLs: a highly significant QTL on Chromosome (Chr) 2, which was common in
both sexes; two significant QTLs on Chr 13, one male-specific and the other female-specific; and a suggestive male-specific
QTL on X Chr. By composite interval mapping, we confirmed the presence of the three QTLs on Chrs 2 and 13, but not of the
male-specific X-linked QTL. The composite interval mapping analysis newly identified three QTLs: a significant male-specific
QTL on Chr 11 and two highly significant female-specific QTLs on Chrs 9 and X. Individual QTLs explained 3.8–11.6% of the
phenotypic variance, and all the QTL alleles derived from the wild mice decreased body weight. A two-way analysis of variance
revealed a significant epistatic interaction between the Chr 2 QTL and the background marker locus D12Mit4 on Chr 12 only in males. The interaction effect unexpectedly increased body weight. The chromosomal region containing the
Chr 2 QTL did not coincide with those of growth or fatness QTLs mapped in previous studies. These results suggest that a population
of wild mice may play an important role as new sources of valuable QTLs.
Received: 14 January 2000 / Accepted: 14 April 2000 相似文献
5.
Detection of quantitative trait loci for meat quality in a commercial slaughter pig cross 总被引:10,自引:0,他引:10
The primary goal of this study was to localize quantitative trait loci (QTL) affecting meat quality traits in swine. In total,
42 traits were scored on 305 F2 individuals from a commercial slaughter pig cross in Norway. F1 and F2 individuals were genotyped for 29 markers on Chromosomes (Chrs) 4, 6, and 7, since previous studies had revealed QTL affecting
meat quality traits on these chromosomes. The most evident result was detection of a QTL affecting amount of intramuscular
fat on Chr 6. The QTL might also influence tenderness, whereas no effect was observed for back-fat thickness. Additionally,
suggestive evidence for QTL affecting other meat quality traits was found on Chr 4 and Chr 7.
Received: 16 June 2000 / Accepted: 5 December 2000 相似文献
6.
Rea Victoria P Anunciado Masahiko Nishimura Masayuki Mori Akira Ishikawa Shin Tanaka Fumihiko Horio Tamio Ohno Takao Namikawa 《Experimental Animals》2003,52(1):37-42
Quantitative trait locus (QTL) analysis of serum insulin, triglyceride, total cholesterol and phospholipid levels at 10 weeks of age was performed in 321 F2 offspring from SM/J and A/J mice. Interval mapping revealed a total of 22 suggestive QTLs affecting the four traits: two insulin QTLs on Chromosomes (Chrs) 6 and 8; six triglyceride QTLs on Chrs 4, 8, 9, 11, 12 and 19; six total-cholesterol QTLs on Chrs 1, 3, 4, 14, 17 and 19; and eight phospholipid QTLs on Chrs 2, 3, 4, 6, 8, 10 and 19. Gender influenced the expression of eight of the suggestive QTLs. The total-cholesterol QTLs on Chrs 4, 14 and 17, the triglyceride QTL on Chr 9 and the phospholipid QTL on Chr 4 were specific to females. The phospholipid QTLs on Chrs 2 and 6 and the insulin QTL on Chr 8 were specific to males. In addition, common QTLs involved in the regulation of some of the traits were identified. The female-specific QTL on Chr 4 appeared to be involved in the regulation of total cholesterol and phospholipid levels. The QTL on Chr 8 affected insulin and phospholipid levels, whereas the Chr 19 QTL was common to the three lipid parameters. 相似文献
7.
Growth control is of fundamental importance to biology in general and of critical importance to cancer research in particular.
Tumors develop when control of the normal growth process is lost. The rat pituitary is a model system for control of estrogen-dependent
growth. Chronic estrogen treatment induces uncontrolled growth in the pituitaries of Fischer 344 (F344) rats, but not of Brown
Norway (BN) rats. We have identified five quantitative trait loci (QTL) for estrogen-dependent pituitary mass (Edpm) in an F2 intercross of F344 and BN. These QTL reside on rat Chromosomes (Chrs) 2, 3, 5, and 9 and explain a total of 55% of the genetic
variance in the F2. We have also detected suggestive evidence for a QTL on rat Chr 14. For Edpm2-1, Edpm2-2, Edpm3, and Edpm5, the F344 allele corresponds with increased pituitary mass, as expected. Surprisingly, for Edpm9 and the suggested QTL on Chr 14, the BN allele corresponds with increased pituitary mass. We also find evidence for interaction
(epistasis) between Edpm3 and Edpm9 and between Edpm5 and the suggested QTL on Chr 14.
Received: 3 March 1997 / Accepted: 1 July 1997 相似文献
8.
Shin-hyo Lee Osamu Ichii Saori Otsuka Yoshiharu Hashimoto Yasuhiro Kon 《Mammalian genome》2010,21(3-4):162-171
MRL/MpJ (MRL) is a model mouse for autoimmune diseases such as dermatitis, vasculitis, arthritis, and glomerulonephritis. In addition to these immune-associated disorders, we found that older MRL mice develop ovarian cysts originating from the rete ovarii, which is lined by ciliated or nonciliated epithelium and considered remnants of mesonephric tubules. Ovarian cysts, which are reported to have several sources, are associated with female infertility, but information regarding the genetic etiology of ovarian cysts originating from the rete ovarii is rare. In this study, to elucidate the genetic background of development of ovarian cysts, we performed quantitative trait locus (QTL) analysis using 120 microsatellite markers, which cover the whole genome of murine chromosomes, and 213 backcross progenies between female MRL and male C57BL/6N mice. The quantitative trait measured was the circumferences of rete ovarii or ovarian cysts. As a result, suggestive linkages were detected on Chrs 3, 4, 6, and 11, but significant linkages were located on Chr 14 by interval mapping. We thereby designated the 27.5-cM region of Chr 14 “MRL Rete Ovarian Cysts (mroc).” The peak regions of Chrs 4 and 14 in particular showed a close additive interaction (p < 0.00001). From these results we concluded that multiple loci on Chrs 3, 4, 6, 11, and 14 interact to result in development of ovarian cysts in MRL mice. 相似文献
9.
Kathryn Gill Nicolas Desaulniers Paule Desjardins Kristine Lake 《Mammalian genome》1998,9(12):929-935
The purpose of the present study was to characterize the C57BL/6J, A/J, and AXB/BXA Recombinant Inbred (RI) strains of mice
for voluntary alcohol consumption. Quantitative Trait Locus (QTL) analysis was used to provide provisional location of QTLs
for alcohol consumption. The inbred strains were screened for levels of alcohol intake (calculated as alcohol preference and
absolute alcohol consumption) by receiving 4 days of forced exposure to a 10% (wt/vol) solution of alcohol, followed by 3
weeks of free choice between water and 10% alcohol. A wide and continuous distribution of values for alcohol consumption and
preference was obtained in the AXB/BXA RI strains, confirming polygenic influences on alcohol-related behaviors. Significant
gender differences were found for both alcohol preference [F28,651= 2.12, p < 0.001] and absolute alcohol consumption [F28,647= 2.57, p < 0.001]. In males, putative QTLs were mapped to chromosomes (Chrs) 2, 5, 7, 10, 11, and 16. Multiple regression analysis
indicated that approximately 75% of the genetic variance in alcohol preference in males could be accounted for by three of
the QTL regions. Several of the putative QTLs appeared to be male-specific (Tyr on Chr 7; D10Mit126 on Chr 10; D11Mit61 on Chr 11). In females, seven putative QTLs were mapped to Chrs 2, 4, 5, 7, 11, 16, and 19. Approximately 90% of the genetic
variance in alcohol preference in females could be accounted for by four QTL regions, as determined by multiple regression.
The QTL on Chr 11 near D11Mit35 appeared to be female-specific. This site was close to a female-specific QTL (Alcp2) previously mapped in C57BL/6J × DBA/2J backcrosses by Melo and coworkers (Nat Genet 13, 147, 1996). The QTLs mapped for
alcohol preference in the present study must be considered suggestive at the present time, since only D2Mit74 met very strict statistical criteria for significance. However, the concordance across several studies for the loci on Chrs
2, 4, 7, 9, and 11 suggest that some common QTLs influencing alcohol preference have been identified. Confirmation of QTLs
mapped in the present study is currently being conducted in a new series of recombinant congenic (RC) strains developed from
reciprocal backcrosses between the A/J and C57BL/6J progenitors. The concomitant use of both RI and RC strains developed from
the same progenitors should provide a powerful means of detecting, confirming, and mapping QTLs for alcohol-related traits.
Received: 25 August 1998 / Accepted: 8 October 1998 相似文献
10.
Eri Nishihara Shirng-Wern Tsaih Chieko Tsukahara Sarah Langley Susan Sheehan Keith DiPetrillo Satoshi Kunita Ken-ichi Yagami Gary A. Churchill Beverly Paigen Fumihiro Sugiyama 《Mammalian genome》2007,18(8):573-583
In a previous study in 15 inbred mouse strains, we found highest and lowest systolic blood pressures in NZO/HILtJ mice (metabolic
syndrome) and C3H/HeJ mice (common lean strain), respectively. To identify the loci involved in hypertension in metabolic
syndrome, we performed quantitative trait locus (QTL) analysis for blood pressure with direction of cross as a covariate in
segregating F2 males derived from NZO/HILtJ and C3H/HeJ mice. We detected three suggestive main-effect QTLs affecting systolic and diastolic
blood pressures (SBP and DBP). We analyzed the first principle component (PC1) generated from SBP and DBP to investigate blood
pressure. In addition to all the suggestive QTLs (Chrs 1, 3, and 8) in SBP and DBP, one suggestive QTL on Chr 4 was found
in PC1 in the main scan. Simultaneous search identified two significant epistatic locus pairs (Chrs 1 and 4, Chrs 4 and 8)
for PC1. Multiple regression analysis revealed three blood pressure QTLs (Bpq10, 100 cM on Chr 1; Bpq11, 6 cM on Chr 4; Bpq12, 29 cM on Chr 8) accounting for 29.4% of blood pressure variance. These were epistatic interaction QTLs constructing a small
network centered on Chr 4, suggesting the importance of genetic interaction for development of hypertension. The blood pressure
QTLs on Chrs 1, 4, and 8 were detected repeatedly in multiple studies using common inbred nonobese mouse strains, implying
substantial QTL independent of development of obesity and insulin resistance. These results enhance our understanding of complicated
genetic factors of hypertension in metabolic diseases.
Eri Nishihara, Shirng-Wern Tsaih, Chieko Tsukahara and Sarah Langley contributed equally to this work. 相似文献
11.
Michal Pravenec Václav Zídek Alena Musilová Miroslava Simáková Vlastimil Kostka Petr Mlejnek Vladimír Kren Drahomíra Krenova Vlasta Bílá Blanka Míková Marie Jáchymová Karel Horký Ludmila Kazdová Elizabeth St. Lezin Theodore W. Kurtz 《Mammalian genome》2002,13(5):253-258
Abnormalities in carbohydrate and lipid metabolism are common in patients with essential hypertension and in the spontaneously
hypertensive rat (SHR). To identify chromosome regions contributing to this clustering of cardiovascular risk factors in the
SHR, we searched for quantitative trait loci (QTL) associated with insulin resistance, glucose intolerance, and dyslipidemia
by using the HXB/BXH recombinant inbred (RI) strains. Analysis of variance in RI strains suggested significant effects of
genetic factors. A genome screening of the RI strains with more than 700 markers revealed QTL significantly associated with
insulin resistance on Chromosomes (Chrs) 3 and 19. The Chr 19 QTL was confirmed by testing a previously derived SHR-19 congenic
strain: transfer of a Chr 19 segment delineated by markers D19Rat57 and D19Mit7 from the Brown Norway (BN/Cr) strain onto the genetic background of the SHR/Ola was associated with decreased insulin and
glucose concentrations and ameliorated insulin resistance at the tissue level. These findings suggest that closely linked
genes on Chr 19, or perhaps even a single gene with pleiotropic effects, influence the clustering of metabolic disturbances
in the SHR-BN model. 相似文献
12.
T. Agui T. Miyamoto C.-G. Jung T. Tsumagari K. Masuda T. Manabe 《Mammalian genome》2000,11(10):862-865
The LEC rat has been reported to exhibit X-ray hypersensitivity and deficiency in DNA double-strand break (DSB) repair. The
present study was performed to map the locus responsible for this phenotype, the xhs (X-ray hypersensitivity), as the first step in identifying the responsible gene. Analysis of the progeny of (BN × LEC)F1× LEC backcrosses indicated that the X-ray hypersensitive phenotype was controlled by multiple genetic loci in contrast to
the results reported previously. Quantitative trait loci (QTL) linkage analysis revealed two responsible loci located on Chromosomes
(Chr) 4 and 1. QTL on Chr 4 exhibited very strong linkage to the X-ray hypersensitive phenotype, while QTL on Chr 1 showed
weak linkage. The Rad52 locus, mutation of which results in hypersensitivity to ionizing radiation and impairment of DNA DSB repair in yeast, was
reported to be located on the synteneic regions of mouse Chr 6 and human Chr 12. However, mapping of the rat Rad52 locus indicated that it was located 23 cM distal to the QTL on Chr 4. Furthermore, none of the radio-sensitivity-related
loci mapped previously in the rat chromosome were identical to the QTL on Chrs 4 and 1 in the LEC rat. Thus, it seems that
X-ray hypersensitivity in the LEC rat is caused by mutation(s) in as-yet-undefined genes.
Received: 14 February 2000 / Accepted: 17 May 2000 相似文献
13.
Quantitative trait loci (QTLs) affecting body weight were investigated in the backcross population derived from nondiabetic
BB/OK and spontaneously hypertensive rat (SHR) strains. The F1 hybrids were backcrossed onto SHR rats, and QTL analysis was performed separately with the resulting backcross populations
for each sex on Chromosomes (Chrs) 1, 3, 4, 10, 13, and 18. The body weight was determined at the age of 14 weeks, and the
statistical analysis was performed with MAPMAKER/QTL 1.1b computer program. According to the stringent threshold for a lod
score of 3.0, markers on Chr 1 were found to be linked with body weight. The QTL with a peak lod score (5.1) on Chr 1 for
a male population was located within markers Igf2 and D1Mgh12. In contrast, in the female population the body weight affecting QTL (lod = 5.7) on Chr 1 was located between the D1Mit3 and Lsn markers. The existence of QTLs on Chr 1 affecting body weight in the male population was confirmed by congenic BB.Sa rats,
carrying chromosomal region of SHR (Sa-Igf2) on the genetic background of BB rat.
Received: 14 July 1997 / Accepted: 22 December 1997 相似文献
14.
A genome-wide scan was performed in order to identify Quantitative Trait Loci (QTL) associated with growth in a population
segregating high growth (hg), a partially recessive mutation that enhances growth rate and body size in the mouse. A sample of 262 hg/hg mice was selected from a C57BL/6J-hg/hg× CAST/EiJ F2 cross and typed with 79 SSLP markers distributed across the genome. Eight significant loci were identified through interval
mapping. Loci on Chromosomes (Chrs) 2 and 8 affected the growth rate of F2 mice. Loci on Chr 2 and 11 affected growth rate and carcass lean mass (protein and ash). A locus on Chr 9 modified femur
length and another one in Chr 17 affected both carcass lean mass and femur length, but none of these had significant effects
on growth rate. Loci on Chrs 5 and 9 modified carcass fat content. Additive effects were positive for C57BL/6J alleles, except
for the two loci affecting carcass fatness. Typing of selected markers in 274 +/+ F2 mice revealed significant interactions between hg and other growth QTL, which were detected as changes in gene action (additive or dominant) and in allele substitution effects.
Knowledge about interactions between loci, especially when major genes are involved, will help in the identification of positional
candidate genes and in the understanding of the complex genetic regulation of growth rate and body size in mammals.
Received: 29 June 2000 / Accepted: 22 November 2000 相似文献
15.
Cécile Vassort Michèle Rivière Gilles Bruneau François Gros Jean Thibault Göran Levan Josiane Szpirer Claude Szpirer 《Mammalian genome》1993,4(4):202-206
By use of rat cDNA probes and a panel of cell hybrids segregating rat chromosomes, the genes encoding three pyridoxal 5-phosphate (PLP)-dependent decarboxylases—namely, DOPA-decarboxylase (Ddc), glutamic acid decarboxylase 1 and 2 (Gad1 and Gad2)—were assigned to rat Chromosomes (Chrs) 14, 3, and 17, respectively. If one takes into account chromosome localizations in the human and the mouse, the present results (i) show that a synteny group is retained on rat Chr 14, human Chr 7, and mouse Chr 11 (Ddc); (ii) strengthen the homology relation known between rat Chr 3 and human and mouse Chrs 2 (Gad1); (iii) suggest that rat Chr 17 has no extensive homology to any human chromosome; and (iv) suggest the order (Prl, Fdp)-Tpl2-Gad2 on the rat Chr 17. 相似文献
16.
Nicola H. Chapman Annette Estes Jeff Munson Raphael Bernier Sara J. Webb Joseph H. Rothstein Nancy J. Minshew Geraldine Dawson Gerard D. Schellenberg Ellen M. Wijsman 《Human genetics》2011,129(1):59-70
Performance IQ (PIQ) greater than verbal IQ (VIQ) is often observed in studies of the cognitive abilities of autistic individuals.
This characteristic is correlated with social and communication impairments, key parts of the autism diagnosis. We present
the first genetic analyses of IQ discrepancy (PIQ–VIQ) as an autism-related phenotype. We performed genome-wide joint linkage
and segregation analyses on 287 multiplex families, using a Markov chain Monte Carlo approach. Genetic data included a genome-scan
of 387 micro-satellite markers in 210 families augmented with additional markers added in a subset of families. Empirical
P values were calculated for five interesting regions. Linkage analysis identified five chromosomal regions with substantial
regional evidence of linkage; 10p12 [P = 0.001; genome-wide (gw) P = 0.05], 16q23 (P = 0.015; gw P = 0.53), 2p21 (P = 0.03, gw P = 0.78), 6q25 (P = 0.047, gw P = 0.91) and 15q23-25 (P = 0.053, gw P = 0.93). The location of the chromosome 10 linkage signal coincides with a region noted in a much earlier genome-scan for
autism, and the chromosome 16 signal coincides exactly with a linkage signal for non-word repetition in specific language
impairment. This study provides strong evidence for a QTL influencing IQ discrepancy in families with autistic individuals
on chromosome 10, and suggestive evidence for a QTL on chromosome 16. The location of the chromosome 16 signal suggests a
candidate gene, CDH13, a T-cadherin expressed in the brain, which has been implicated in previous SNP studies of autism and
ADHD. 相似文献
17.
Marker–quantitative trait locus (QTL) linkage was evaluated in F2 intercross and backcross mouse populations derived from stocks differing dramatically in prolificacy and mature weight. A
highly prolific outbred Quackenbush-Swiss mouse line, or an inbred line derived from it (16.62 ± 0.22 and 14.64 ± 0.27 pups
per litter, respectively) were used as one of the grandparents in these populations. The less prolific C57BL/6J inbred mouse
line (6.67 ± 0.37 pups per litter) was used as the other grandparent. Linkage was evaluated in a three-step process that involved
selective genotyping of F2 intercross progeny representing extremes for prolificacy, genotyping of the full F2 for chromosomal regions potentially associated with prolificacy, and genotyping of the backcross for genomic regions significantly
associated with prolificacy in the F2. Segments of Chromosomes (Chrs) 2 and 4 were significantly (P < 0.05, experiment-wise error rate) associated with prolificacy,
and LOD scores suggestive of linkage were observed for litter size on Chr 9 and growth on Chrs 4 and 11. Existence of growth
QTL was also supported by marker effects that were significant (P < 0.05) or approaching significance (P < 0.10) in the backcross.
Additive litter size QTL effects ranged from 0.56 to 0.79 pups per litter, and dominance deviations ranged from −0.56 to 1.19
pups per litter, suggesting overdominance as a possible mode of gene action in some cases. The observation of pleiotropic
or linked QTL for growth and prolificacy corresponds well with results from many selection experiments identifying positively
correlated responses to selection for these two traits.
Received: 9 August 1997 / Accepted: 30 September 1997 相似文献
18.
Jun-ichi Suto Saori Matsuura Kenkichi Imamura Harumichi Yamanaka Kenji Sekikawa 《Mammalian genome》1998,9(7):506-510
KK mouse is known as a polygenic model for non-insulin-dependent diabetes mellitus with moderate obesity. To identify the
quantitative trait loci (QTLs) responsible for the body weight in KK, linkage analysis with 97 microsatellite markers was
carried out into 192 F2 progeny, comprising 93 mice with a/a genotype at agouti locus and 99 mice with A
y
/a genotype, of a cross between C57BL/6J female and KK-Ay (Ay congenic) male, thereby the influence of A
y
allele on the quantitative regulation of body weight was also examined. In F2
a/a mice, we identified a QTL on Chromosome (Chr) 4, and two loci with suggestive linkage on Chrs 15 and 18. In F2
A
y
/a mice, a QTL was identified on Chr 6, and two loci with suggestive linkage were identified on Chrs 4 and 16. That the QTL
on Chr 4 was held in common between F2
a/a and F2
A
y
/a progenies implies that this locus may be a primary component regulating body weight in KK and KK-Ay. These results suggest that the body weight in KK is controlled by multiple genes, and the different combination of loci
is involved in the presence of A
y
allele. The QTL on Chr 6 seemed to determine the body weight by controlling fat deposition, because the linkage was identified
on body weight and adiposity, and is suggested to be a component involved in the metabolic pathway in obesity caused by the
A
y
allele.
Received: 16 December 1997 / Accepted: 16 March 1998 相似文献
19.
QTL identification of flowering time at three different latitudes reveals homeologous genomic regions that control flowering in soybean 总被引:1,自引:0,他引:1
Liu W Kim MY Kang YJ Van K Lee YH Srinives P Yuan DL Lee SH 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(4):545-553
Since the genetic control of flowering time is very important in photoperiod-sensitive soybean (Glycine max (L.) Merr.), genes affecting flowering under different environment conditions have been identified and described. The objectives
were to identify quantitative trait loci (QTLs) for flowering time in different latitudinal and climatic regions, and to understand
how chromosomal rearrangement and genome organization contribute to flowering time in soybean. Recombinant inbred lines from
a cross between late-flowering ‘Jinpumkong 2’ and early-flowering ‘SS2-2’ were used to evaluate the phenotypic data for days
to flowering (DF) collected from Kamphaeng Saen, Thailand (14°01′N), Suwon, Korea (37°15′N), and Longjing, China (42°46′N).
A weakly positive phenotypic correlation (r = 0.36) was found between DF in Korea and Thailand; however, a strong correlation (r = 0.74) was shown between Korea and China. After 178 simple sequence repeat (SSR) markers were placed on a genetic map spanning
2,551.7 cM, four independent DF QTLs were identified on different chromosomes (Chrs). Among them, three QTLs on Chrs 9, 13
and 16 were either Thailand- or Korea-specific. The DF QTL on Chr 6 was identified in both Korea and China, suggesting it
is less environment-sensitive. Comparative analysis of four DF QTL regions revealed a syntenic relationship between two QTLs
on Chrs 6 and 13. All five duplicated gene pairs clustered in the homeologous genomic regions were found to be involved in
the flowering. Identification and comparative analysis of multiple DF QTLs from different environments will facilitate the
significant improvement in soybean breeding programs with respect to control of flowering time. 相似文献
20.
Amy F. Eisener-Dorman Laura Grabowski-Boase Brian M. Steffy Tim Wiltshire Lisa M. Tarantino 《Mammalian genome》2010,21(5-6):231-246
Quantitative trait locus (QTL) mapping in the mouse typically utilizes inbred strains that exhibit significant genetic and phenotypic diversity. The development of dense SNP panels in a large number of inbred strains has eliminated the need to maximize genetic diversity in QTL studies as plenty of SNP markers are now available for almost any combination of strains. We conducted a QTL mapping experiment using both a backcross (N2) and an intercross (F2) between two genetically similar inbred mouse strains: C57BL/6J (B6) and C57L/J (C57). A set of additive QTLs for activity behaviors was identified on Chrs 1, 9, 13, and 15. We also identified additive QTLs for anxiety-related behaviors on Chrs 7, 9, and 16. A QTL on Chr 11 is sex-specific, and we revealed pairwise interactions between QTLs on Chrs 1 and 13 and Chrs 10 and 18. The Chr 9 activity QTL accounts for the largest amount of phenotypic variance and was not present in our recent analysis of a B6 × C58/J (C58) intercross (Bailey et al. in Genes Brain Behav 7:761–769, 2008). To narrow this QTL interval, we used a dense SNP haplotype map with over 7 million real and imputed SNP markers across 74 inbred mouse strains (Szatkiewicz et al. in Mamm Genome 19(3):199–208, 2008). Evaluation of shared and divergent haplotype blocks among B6, C57, and C58 strains narrowed the Chr 9 QTL interval considerably and highlights the utility of QTL mapping in closely related inbred strains. 相似文献