共查询到11条相似文献,搜索用时 0 毫秒
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Some prerequisites for ecological risk assessment of transgenic plant releases are examined, with the aim of identifying research needs in Austria. Austria harbours high habitat diversity, extending from the Alps to the Pannonian continental lowlands. The Pannonian flora includes potential cross-breeding partners of several crops, which do not occur in western Europe. The border zones of major biogeographical regions contain high biodiversity, both in terms of species and habitat diversity; whether the genetic variability of widespread plant species is also increased in these areas requires further investigation. Especially in Alpine regions, transgenic grasses might eventually cause profound changes in community structure. Ecological risk assessment of transgenic crop releases should not only consider adverse effects on non-target species and ecosystems, but also their likely impact on the agroecosystems. 相似文献
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The cultivation of Lepidoptera‐resistant Bt‐maize may affect nontarget butterflies. We assessed the risk posed by event MON89034 × MON88017 (expressing Cry1A.105 and Cry2Ab2 against corn borers) to nontarget Lepidoptera. Using the small tortoiseshell Aglais urticae, a butterfly species common in central Europe, as a test organism we (i) assessed the toxicity of Bt‐maize pollen on butterfly larvae; (ii) measured pollen deposition on leaves of the host plant Urtica dioica; (iii) mapped the occurrence and distribution of host plants and larvae in two arable landscapes in Germany during maize anthesis; and (iv) described the temporal occurrence of a 1‐year population of A. urticae. (i) Larvae‐fed 200 Bt‐maize pollen grains/cm2 had a reduced feeding activity. Significant differences in developmental time existed at pollen densities of 300 Bt‐maize pollen grains/cm2 and in survival at 400 grains/cm2. (ii) The highest pollen amount found was 212 grains/cm2 at the field margin. Mean densities were much lower. (iii) In one region, over 50% of A. urticae nests were located within 5 m of a maize field, while in the other, all nests were found in more than 25 m distance to a maize field. (iv) The percentage of larvae developing during maize anthesis was 19% in the study area. The amount of pollen from maize MON89034 × MON88017 found on host plants is unlikely to adversely affect a significant proportion of larvae of A. urticae. This paper concludes that the risk of event MON89034 × MON88017 to populations of this species is negligible. 相似文献
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Suzuki S Murakami S Tsujimae K Findlay I Kurachi Y 《Progress in biophysics and molecular biology》2008,98(1):52-60
The main components of repolarization reserve for the ventricular action potential (AP) are the rapid (IKr) and slow (IKs) delayed outward K+ currents. While many drugs block IKr and cause life-threatening arrhythmias including torsades de pointes, the frequency of arrhythmias varies between different IKr-blockers. Different types of block of IKr cause distinct phenotypes of prolongation of action potential duration (APD), increase in transmural dispersion of repolarization (TDR) and, accordingly, occurrence of torsades de pointes. Therefore the assessment of a drug's proarrhythmic risk requires a method that provides quantitative and comprehensive comparison of the effects of different forms of IKr-blockade upon APDs and TDR. However, most currently available methods are not adapted to such an extensive comparison. Here, we introduce IKr–IKs two-dimensional maps of APD and TDR as a novel risk-assessment method. Taking the kinetics of IKr-blockade into account, APDs can be calculated upon a ventricular AP model which systematically alters the magnitudes of IKr and IKs. The calculated APDs are then plotted on a map where the x axis represents the conductance of IKr while the y axis represents that of IKs. TDR is simulated with models corresponding to APs in epicardial, midcardial and endocardial myocardium. These two-dimensional maps of APD and TDR successfully account for differences in the risk resulting from three distinct types of IKr-blockade which correspond to the effects of dofetilide, quinidine and vesnarinone. This method may be of use to assess the arrhythmogenic risk of various IKr-blockers. 相似文献
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Carol Barrette Emily Cobb Ronald McNicol Gary Lyon 《Plant Cell, Tissue and Organ Culture》1997,47(2):135-144
Agrobacterium transformation systems forBrassica, Solanum andRubus, using carbenicillin, cefotaxime and ticaracillin respectively to eliminate contamination, were examined for the presence of residualAgrobacterium. The results indicated that none of the antibiotics in question, succeeded in eliminatingAgrobacterium and the contamination levels increased in explants from 12 to 16 weeks to such an extent thatSolanum cultures senesced and died. This may be due to the fact that four times the Minimum bactericidal concentration values (concentration to be used for elimination of contaminants in culture), for the three antibiotics, were higher than the concentrations employed in the culture medium. Contamination in shoot material decreased over 16 to 24 weeks possibly due to bacteriostatis and the use only of the apical node for further culture. The presence of the binary vector was also noted under non-selective conditions, even up to 6 months after transformation, where approx. 50% of contaminated material still harboured bacterial cells with the binary vector at levels of approx. 107 Colony forming units per gram. 相似文献
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ANDREW CHIN PETER M. KYNE TERENCE I. WALKER RORY B. McAULEY 《Global Change Biology》2010,16(7):1936-1953
An Integrated Risk Assessment for Climate Change (IRACC) is developed and applied to assess the vulnerability of sharks and rays on Australia's Great Barrier Reef (GBR) to climate change. The IRACC merges a traditional climate change vulnerability framework with approaches from fisheries ecological risk assessments. This semi‐quantitative assessment accommodates uncertainty and can be applied at different spatial and temporal scales to identify exposure factors, at‐risk species and their key biological and ecological attributes, critical habitats a`nd ecological processes, and major knowledge gaps. Consequently, the IRACC can provide a foundation upon which to develop climate change response strategies. Here, we describe the assessment process, demonstrate its application to GBR shark and ray species, and explore the issues affecting their vulnerability to climate change. The assessment indicates that for the GBR, freshwater/estuarine and reef associated sharks and rays are most vulnerable to climate change, and that vulnerability is driven by case‐specific interactions of multiple factors and species attributes. Changes in temperature, freshwater input and ocean circulation will have the most widespread effects on these species. Although relatively few GBR sharks and rays were assessed as highly vulnerable, their vulnerability increases when synergies with other factors are considered. This is especially true for freshwater/estuarine and coastal/inshore sharks and rays. Reducing the impacts of climate change on the GBR's sharks and rays requires a range of approaches including mitigating climate change and addressing habitat degradation and sustainability issues. Species‐specific conservation actions may be required for higher risk species (e.g. the freshwater whipray, porcupine ray, speartooth shark and sawfishes) including reducing mortality, preserving coastal catchments and estuarine habitats, and addressing fisheries sustainability. The assessment identified many knowledge gaps concerning GBR habitats and processes, and highlights the need for improved understanding of the biology and ecology of the sharks and rays of the GBR. 相似文献
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Biosafety and risk assessment framework for selectable marker genes in transgenic crop plants: a case of the science not supporting the politics 总被引:1,自引:0,他引:1
Ramessar K Peremarti A Gómez-Galera S Naqvi S Moralejo M Muñoz P Capell T Christou P 《Transgenic research》2007,16(3):261-280
Selectable marker gene systems are vital for the development of transgenic crops. Since the creation of the first transgenic plants in the early 1980s and their subsequent commercialization worldwide over almost an entire decade, antibiotic and herbicide resistance selectable marker gene systems have been an integral feature of plant genetic modification. Without them, creating transgenic crops is not feasible on purely economic and practical terms. These systems allow the relatively straightforward identification and selection of plants that have stably incorporated not only the marker genes but also genes of interest, for example herbicide tolerance and pest resistance. Bacterial antibiotic resistance genes are also crucial in molecular biology manipulations in the laboratory. An unprecedented debate has accompanied the development and commercialization of transgenic crops. Divergent policies and their implementation in the European Union on one hand and the rest of the world on the other (industrialized and developing countries alike), have resulted in disputes with serious consequences on agricultural policy, world trade and food security. A lot of research effort has been directed towards the development of marker-free transformation or systems to remove selectable markers. Such research has been in a large part motivated by perceived problems with antibiotic resistance selectable markers; however, it is not justified from a safety point of view. The aim of this review is to discuss in some detail the currently available scientific evidence that overwhelmingly argues for the safety of these marker gene systems. Our conclusion, supported by numerous studies, most of which are commissioned by some of the very parties that have taken a position against the use of antibiotic selectable marker gene systems, is that there is no scientific basis to argue against the use and presence of selectable marker genes as a class in transgenic plants. 相似文献
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Duane A. Tolle David J. Hesse G. Bradley Chadwell Joyce S. Cooper David P. Evers 《The International Journal of Life Cycle Assessment》2001,6(2):96-105
Three approaches recommended for characterization of toxicity impact potential in a life cycle impact assessment (LCIA) are
tested on a case study and compared. The two equivalency factor methods are the Persistence, Bioaccumulation, and Toxicity
(PBT) method and the Multimedia Fate Modeling (MFM) method using a Mackay Level III model with state-specific environmental
data. The simplified risk assessment (SRA) method involved dispersion modeling using site-specific environmental data. The
life cycle inventory information evaluated by all three methods was limited to manufacturing of the RDX-based explosive in
Kingsport, Tennessee. The effort to collect site-specific environmental data and conduct air dispersion modeling for the SRA
method required about 24 times more effort than the PBT method and about 4 times more effort than the MFM method. Direct comparison
of impact potential scores for the three approaches were limited to inhalation toxicity scores for nine air pollutants modeled
by SRA. Correlations were made on the rank order of the impact potential scores for the nine air emissions evaluated for all
three LCIA methods. Although the number of chemicals compared is very limited, the best correlation coefficient (0.96) was
between the rank orders for the MFM and the SRA methods. The minimal effort and reduced accuracy of the PBT approach make
it best suited for screening large numbers of chemicals for further evaluation of the highest ranked chemicals. The intermediate
effort and reasonable accuracy (includes transfers to other media) of the MFM approach make it well suited for LCIAs involving
comparative assertions or governmental policy decisions. The maximum effort and assumption of highest accuracy make the SRA
approach suitable only after limiting the locations of interest to a few sites by screening with the other two approaches. 相似文献
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