共查询到20条相似文献,搜索用时 46 毫秒
1.
Araki M Nagayama T 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》2012,198(8):607-615
The biogenic amines, octopamine and serotonin, modulate the synaptic activity of the lateral giant interneuron (LG) circuitry of the crayfish escape behavior. Bath application of both octopamine and serotonin enhances the synaptic responses of LG to sensory stimulation. We have shown previously (Araki et al. J Neurophysiol 94:2644-2652, 2005) that a serotonin-induced enhancement of the LG response was mediated by an increase in cAMP levels following activation of adenylate cyclase; however, octopamine acts independently. Here, we clarify how octopamine enhances the LG response during sensory stimulation using physiological and pharmacological analyses. When phospholipase C inhibitor U-73122 was directly injected into the LG before biogenic amine application, it abolished the enhancing effect of octopamine on direct sensory input to the LG, but did not block indirect input via sensory interneurons or the effect of serotonin. Direct injection of IP(3), and its analogue adenophostin A, into the LG increased the synaptic response of the LG to sensory stimulation. Thus, IP(3) mediates octopamine-induced synaptic enhancement of the LG, but serotonin acts independently. These results indicate that both octopamine and serotonin enhance the synaptic responses of the LG to sensory stimulation, but that they activate two different signaling cascades in the LG. 相似文献
2.
Habituation (extinction) of postsynaptic unit responses of the cat motor cortex to repetitive electrodermal, photic, acoustic, and combined bimodal stimulation was investigated by intracellular recording. Habituation was shown by a decrease in the number of spikes per grouped discharge and a decrease in the amplitude and duration of the EPSPs, and sometimes IPSPs, on repetition of the stimulus. The way in which the course of habituation depends on the modality and duration of stimulation (at a constant frequency of 1/sec) is examined. Habituation of postsynaptic responses to sensory stimuli is observed with neurons of different functional groups, namely identified neurons of pyramidal tract and unidentified neurons, some of which were evidently pyramidal neurons and interneurons. The hypothesis is put forward that the habituation of PSPs of the cortical neurons is based on processes taking place mainly at the subcortical level.A. A. Zhdanov Leningrad State University. Translated from Neirofiziologiya, Vol. 4, No. 5, pp. 545–553, September–October, 1972. 相似文献
3.
Changes in spike potentials and EPSPs and IPSPs of neurons in the general cortex of the turtle forebrain were investigated intracellularly during habituation to flashes. The amplitudes of all these potentials were reduced although the level of the membrane potential remained unchanged. Their dependence on membrane potential was disturbed. The lowering of amplitude of the short-latency spike in response to flashes was greater than that of the spontaneous spike or of the spike after an IPSP. Considering that with extracellular recording only a selective lowering of the short-latency spike is observed, it can be concluded that depression of the spontaneous spike and of the post-IPSP spike reflects a nonspecific decrease in neuron excitability on account of prolonged intracellular recording, whereas the lowering of the short-latency spike reflects habituation at the neuronal level. Disinhibition of the amplitude of spikes and postsynaptic potentials was observed. The hypothesis that a population of synapses activated by a particular stimulus when applied repeatedly induces a short-term change in the electrogenic prperties of the nonreceptor neuron membrane, which determines the depression of the electrical responses, is put forward and discussed.M. V. Lomonosov State University, Moscow. Translated from Neirofiziologiya, Vol. 8, No. 1, pp. 22–29, January–February, 1976. 相似文献
4.
The neural circuit that underlies the lateral giant fiber (LG)-mediated reflex escape in crayfish has provided findings relating synaptic change to nonassociative learning such as sensitization and habituation. The LGs receive sensory inputs from the primary sensory afferents and a group of mechanosensory interneurons (MSIs). An increase of excitability by suprathreshold repetitive excitation of this circuit, which is similar to Hebbian long-term potentiation (LTP), has been reported [Miller et al. (1987) J Neurosci 7:1081]. This potentiation was previously thought to result from the enhancement of transmission at cholinergic synapses between primary afferents and MSIs but not the electrical synapses onto LG. In this study, we found that potentiation of synaptic signaling at the electrical synapse onto LG can also be induced when the synapse was activated with subthreshold repetitive pulses or with a few strong suprathreshold shocks. LG LTP was induced in the preparation which had received pulses at limited frequency range. Although whether this LTP is involved in the learning process of escape behavior in crayfish is not clear, the intensity and amount of sensory stimulation used here mimicked those that could easily be produced by a predator trying to catch a crayfish and could be of adaptive significance in life. 相似文献
5.
M. G. Moldavan 《Neurophysiology》1991,23(2):145-153
During heteromodal extraneous stimulation (ES), a large part of responding neurons of the thalamic reticular nucleus (RN) exhibit selectivity by responding with excitation to the stimulation of only one type of sensory input. To visual ES, 12 of 32 tested neurons responded; and 4 of 21 tested neurons responded to auditory ES. Response of neurons to ES diminished during the process of habituation to these stimuli; and after habituation was completed, the number of neurons responsive to the ES also diminished. Use of ES led to disappearance of high-frequency, grouped discharges in the responses of the RN neurons. Initial responses to ES and to the following conditioning stimulus (CS) appeared during external inhibition of the conditioned reflex (CR), but the later components of impulse responses that ordinarily accompany realized CR were suppressed in nearly one half of the studied neurons. We reach the conclusion that RN neurons participate in external inhibition of CR and in habituation to ES.A. A. Bogomolets Physiology Institute, Academy of Sciences of the USSR, Kiev. Translated from Neirofiziologiya, Vol. 23, No. 2, pp. 189–199, March–April, 1991. 相似文献
6.
Neuromodulators orchestrate complex behavioral routines by their multiple and combined effects on the nervous system. In the desert locust, Schistocerca gregaria, frontal ganglion neurons innervate foregut dilator muscles and play a key role in the control of foregut motor patterns. To further investigate the role of the frontal ganglion in locust behavior, we currently focus on the frontal ganglion central pattern generator as a target for neuromodulation. Application of octopamine, a well-studied insect neuromodulator, generated reversible disruption of frontal ganglion rhythmic activity. The threshold for the modulatory effects of octopamine was 10–6 mol l–1, and 10–4 mol l–1 always abolished the ongoing rhythm. In contrast to this straightforward modulation, allatostatin, previously reported to be a myoinhibitor of insect gut muscles, showed complex, tri-modal, dose-dependent effects on frontal ganglion rhythmic pattern. Using a novel cross-correlation analysis technique, we show that different allatostatin concentrations have very different effects not only on cycle period but also on temporal characteristics of the rhythmic bursts of action potentials. Allatostatin also altered the frontal ganglion rhythm in vivo. The analysis technique we introduce may be instrumental in the study of not fully characterized neural circuits and their modulation. The physiological significance of our results and the role of the modulators in locust behavior are discussed.Abbreviation CPG central pattern generator - FG frontal ganglion - JH juvenile hormone - STNS stomatogastric nervous system 相似文献
7.
Tetraploid plants were obtained in pomegranate (Punica granatum L. var. `Nana') by colchicine treatment of shoots propagated in vitro. Shoots cultured on MS medium supplemented with 10 mg l–1 colchicine, 1.0 mg l–1 BA and 0.1 mg l–1 NAA for 30 days produced tetraploids at a high frequency of 20%. No tetraploids were detected by treating the shoots in 5000 mg l–1 colchicine for 114 h. Shoots treated by 5000 mg l–1 colchicine for 96 h produced three morphological mutants with narrow leaves, which were later confirmed as mixoploids that separated into diploids and tetraploids after further subculture. In vitro tetraploid plants had shorter roots, wider and shorter leaves than the diploid ones. Tetraploid pomegranate plants grew and flowered normally in pots, but possessed flowers with increased diameter and decreased length compared to diploids. The number of pollen grains per anther was higher in tetraploids, but the viability of pollen decreased significantly. 相似文献
8.
Habituation of the tentacle retraction reflex was studied at the following response levels: (1) Muscle tension elicited in the tentacle retractor muscle by repeated stimulation of a cerebral nerve (at 60-sec intervals) declined in parallel with evoked activity of the largest unit in the tentacle retractor nerve. (2) The largest unit in the tentacle retractor nerve (L4) showed spontaneous recovery and dishabituation. The rate of response decrement was inversely related to the strength of stimulus, and an optimal interstimulus interval ca. 60 s was found. Retention of habituation for 24 h was exhibited. (3) The major retractor motoneurons (L2, L3, L4) all showed habituation, dishabituation, and spontaneous recovery. The decline of L4 activity was parallelled by a decline in muscle response. (4) Compound EPSPs elicited in the retractor motoneurons by stimulation of sensory pathways showed habituation and dishabituation. (5) Unitary EPSPs elicited by stimulation of cerebral nerves and connectives with minimal stimulus strengths also showed habituation and were unaffected by spontaneously occurring EPSPs. Dishabituation by another pathway was also shown. (6) Depolarization of L4 by a constant current produced spike trains of constant firing rate and evoked a constant level of muscle tension in repeated trials, suggesting the absence of habituation in a peripheral nerve net or at the neuromuscular junction. 相似文献
9.
Sulfolobus solfataricus used 2-propanol and 2-propanone (acetone) when grown in static cultures at 78 °C with or without glucose at 10 g l–1. The presence of 3.92 g 2-propanol l–1 in both cases inhibited growth. However, acetone accumulation following 2-propanol depletion suggested that 2-propanol was co-metabolized via the acetone metabolic pathway. Glucose at 10 g l–1 increased 2-propanol and acetone utilization from 0.93 g l–1 to 1.77 g l–1 and from 0.11 g l–1 to 1.62 g l–1, respectively. Without glucose, immobilized S. solfataricus cells increased the 2-propanol removal rate to 0.035 g l–1 h–1, compared to 0.0012 g l–1 h–1 by its suspended counterpart. The results suggest the establishment of an immobilized reactor configuration is preferential for the treatment of high temperature solvent waste streams by this acidothermophilic Crenarchaeon. 相似文献
10.
The effects of TEA, 4-AP, Co++, Cd++, Cs+, EDTA, and verapamil on the sensory epithelium of ampullae of Lorenzini were studied inRaja clavata (Black Sea skate). During voltage clamping, transepithelial application of TEA to the basal surface caused oscillations in transepithelial potentials in response to presentation of an excitatory stimulus, which had been suppressed by Co++, Cd++, and EDTA. Application of Cs+ was followed by complete or partial suppression of spike response adaptation. When applied to the apical epithelial surface, TEA produced an increase in spike response to stimulation and highly accelerated adaption. No substantial changes took place after apical application of Co++, Cd++, and verapamil. The mechanisms of these phenomena are discussed.Deceased.I. P. Pavlov Institute of Physiology, Academy of Sciences of the USSR, Leningrad. I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Academy of Sciences of the USSR, Leningrad. Translated from Neirofiziologiya, Vol. 17, No. 5, pp. 652–659, September–October, 1985. 相似文献
11.
BackgroundMindfulness as a mode of sustained and receptive attention promotes openness to each incoming stimulus, even if repetitive and/or aversive. Mindful attention has been shown to attenuate sensory habituation in expert meditators; however, others were not able to replicate this effect. The present study used acoustic startle reflex to investigate the effect of mindfulness practice intensity on sensory habituation.MethodsAuditory Startle Response (ASR) to 36 startling probes (12 trials x 3 block with 40ms inter-block intervals), was measured using electromyography (EMG) in three groups of participants (N = 12/group): meditation-naïve, moderate practice, and intensive practice.ResultsIntensive practice group showed attenuated startle habituation as evidenced by significantly less habituation over the entire experiment relative to the meditation-naïve and moderate practice groups. Furthermore, there was a significant linear effect showing between-block habituation in meditation-naïve and moderate practice groups, but not in the intensive practice group. However, the Block x Group interaction between the intensive practice and the meditation-naive groups was not significant. Moderate practice group was not significantly different from the meditation-naïve in the overall measure of habituation, but showed significantly stronger habituation than both meditation-naïve and intensive practice groups in Block 1. Greater practice intensity was significantly correlated with slower overall habituation and habituation rate in Blocks 2 and 3 in the intensive, but not in the moderate, practice group.ConclusionsThe study provides tentative evidence that intensive mindfulness practice attenuates acoustic startle habituation as measured by EMG, but the effect is modest. Moderate practice, on the other hand, appears to enhance habituation, suggesting the effect of mindfulness practice on startle habituation might be non-liner. Better understanding of the effect of mindful attention on startle habituation may shed new light on sensory information processing capacity of the human brain and its potential for de-automatisation of hard-wired processes. 相似文献
12.
Effects of acetylcholine, octopamine, and their antagonists, as well as of glutamic acid were studied on contractions of dorsal longitudinal muscle of the mollusc Lymnaea stagnalis L., evoked by electrical stimulation of n. cervicalis inferior. Acetylcholine and octopamine increased amplitude of contractions evoked by applications at concentrations about 10–8 M and decreased at concentrations higher than 10–7 M. Glutamic acid produced only inhibitory effect on the contraction amplitude, which appeared at concentrations beginning from 10–9 M and higher. The acetylcholine antagonists atropine and d-tubocurarine also decreased amplitude of evoked contractions. Their blocking effect rose with increase of their concentrations in the range from 10–9 M to 10–5 M. Specificity of the effect of the studied substances was established in experiments with a combined application of the transmitters and their antagonists. The obtained results indicate multiplicity of chemical mechanisms of regulation of contractions of the dorsal longitudinal muscle in L. stagnalis.Translated from Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, Vol. 41, No. 1, 2005, pp. 44–50.Original Russian Text Copyright © 2005 by Kononenko, Zhukov. 相似文献
13.
Repetitive stimulation of the locus coeruleus with a frequency of 40 Hz and strength of 50–150 µA in decerebellated cats anesthetized with chloralose was accompanied by a decrease in the inhibitory action of flexor reflex afferents (FRA) on the extensor monosynaptic reflex. This effect, which appeared after 600 msec, reached a maximum 1500–1700 msec after the beginning of repetitive stimulation. A minimum of 7–10 stimuli was needed to evoke the effect. After the end of stimulation the inhibitory action of FRA was not fully restored until after 2–3 sec. During application of a single stimulus or a short high-frequency series of stimuli of the same strength to the locus coeruleus no such effect was found. An increase in the strength of stimulation in that case was accompanied by activation of adjacent more rapidly conducting structures. The advantage of repetitive stimulation for detecting effects of slowly-conducting brain structures is discussed.A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 13, No. 2, pp. 187–195, March–April, 1981. 相似文献
14.
John M. Ratcliffe James H. Fullard Benjamin J. Arthur Ronald R. Hoy 《Proceedings. Biological sciences / The Royal Society》2011,278(1704):364-370
Moths and butterflies flying in search of mates risk detection by numerous aerial predators; under the cover of night, the greatest threat will often be from insectivorous bats. During such encounters, the toxic dogbane tiger moth, Cycnia tenera uses the received intensity, duration and emission pattern of the bat''s echolocation calls to determine when, and how many, defensive ultrasonic clicks to produce in return. These clicks, which constitute an acoustic startle response, act as warning signals against bats in flight. Using an integrated test of stimulus generalization and dishabituation, here we show that C. tenera is able to discriminate between the echolocation calls characteristic of a bat that has only just detected it versus those of a bat actively in pursuit of it. We also show that C. tenera habituates more profoundly to the former stimulus train (‘early attack’) than to the latter (‘late attack’), even though it was initially equally responsive to both stimuli. Matched sensory and behavioural data indicate that reduced responsiveness reflects habituation and is not merely attributable to sensory adaptation or motor fatigue. In search of mates in the face of bats, C. tenera''s ability to discriminate between attacking bats representing different levels of risk, and to habituate less so to those most dangerous, should function as an adaptive cost–benefit trade-off mechanism in nature. 相似文献
15.
Lomascolo Anne Lesage-Meessen Laurence Haon Mireille Navarro David Antona Claudine Faulds Craig Marcel Asther 《World journal of microbiology & biotechnology》2001,17(1):99-102
Aspergillus
niger was explored, for the first time, for the production of 2-phenylethanol (a rose-like aroma) using L-phenylalanine as precursor. Among the strains screened, A. niger CMICC 298302 was shown to produce, in a culture medium containing 6 g L-phenylalanine l–1 and 60 g glucose l–1, 1375 mg 2-phenylethanol l–1 with a productivity of 153 mg l–1 day–1 and a molar yield of 74%. 2-Phenylethanol concentrations of 1 to 2 g l–1 led to a two-fold and ten-fold decrease, respectively, in the mycelial radial growth rate. However, 2-phenylethanol was synthesized as the sole aromatic product and accumulated in the culture broth. 相似文献
16.
Several phases were distinguished in single-unit responses in areas 3 and 4 during defensive conditioning to acoustic stimulation: an initial response, short inhibition of the spike discharge, early and late after-discharges, and changes arising after the end of acoustic stimulation. The initial spike response appeared or intensified (if present already) in the first period of defensive conditioning parallel with an increase in spontaneous unit activity. After-discharges appeared later. The conditioned-reflex movement usually began 100–400 msec after stimulation began. This latent period of the first movement was the same whether for a real conditioned reflex or an after-discharge. Comparison of the latent periods of conditioned movements with the phases of the unit responses showed that the conditioned responses of the cortical neuron were primarily modified after-discharges of neurons evoked by a conditioned stimulus. Differential unit responses to acoustic stimulation, also based on after-discharges, were formed just as actively as positive. The basic role of reinforcement during conditioning is not to increase the excitability of the neurons, which is important in connection with their acquisition of polysensory properties, but to modify the after-discharges of the neurons.A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 10, No. 4, pp. 339–347, July–August, 1978. 相似文献
17.
Ibrahim M. Banat Poonam Nigam Roger Marchant 《Applied microbiology and biotechnology》1992,37(3):378-381
Summary A salicylate-hydroxylase-producing strain of Pseudomonas putida with an unusual capability to grow at toxic levels of salicylate up to 10 g l–1 has been isolated. It grew well under continuous culture conditions, with optimum growth at pH 6.5 and a temperature of 25° C. The use of an ammonium salt as a nitrogen source, instead of nitrate, resulted in a 30–40% increase in its biomass yield coefficient. Optimum growth under continuous culture conditions was achieved using 4 g l–1 salicylate at 25° C, pH 6.5 and 0.2 h–1 dilution rate. High salicylate hydroxylase enzyme activity [236 units (U) l–1] and productivity (424.8 U h–1) were obtained at a dilution rate of 0.45 h–1 using a mineral medium containing 4 g l–1 of salicylate. Operating under continuous culture conditions with oxygen limitation and a slight accumulation of residual salicylate (0.2 g l–1) resulted in a decrease in culture performance and enzyme productivity.
Correspondence to: R. Marchant 相似文献
18.
Pluronic F-68, PEG 8000, or PEG 20 000 added to cell suspension cultures of transgenic Nicotiana tabacum promoted cell growth and the production of the recombinant murine granulocyte macrophage-colony stimulating factor (mGM-CSF) in a 5-l stirred tank bioreactor. The specific growth rates were enhanced from 0.27 d–1 to 0.47 d–1, 0.37 d–1 and 0.4 d–1 when Pluronic F-68, PEG 8000, or PEG 20 000 was added, respectively. The maximum cell density was also increased most to 13.6 g l–1 when Pluronic F-68 was added (11.3 g l–1 in the control culture). In terms of mGM-CSF production, PEG 8000 gave the greatest stimulation and with 2 g PEG 8000 l–1, mGM-CSF increased from 1.6 to 6.6 ng ml–1. 相似文献
19.
Siobhan M. Schabrun Michael C. Ridding Mary P. Galea Paul W. Hodges Lucinda S. Chipchase 《PloS one》2012,7(12)
Peripheral electrical stimulation (PES) is a common clinical technique known to induce changes in corticomotor excitability; PES applied to induce a tetanic motor contraction increases, and PES at sub-motor threshold (sensory) intensities decreases, corticomotor excitability. Understanding of the mechanisms underlying these opposite changes in corticomotor excitability remains elusive. Modulation of primary sensory cortex (S1) excitability could underlie altered corticomotor excitability with PES. Here we examined whether changes in primary sensory (S1) and motor (M1) cortex excitability follow the same time-course when PES is applied using identical stimulus parameters. Corticomotor excitability was measured using transcranial magnetic stimulation (TMS) and sensory cortex excitability using somatosensory evoked potentials (SEPs) before and after 30 min of PES to right abductor pollicis brevis (APB). Two PES paradigms were tested in separate sessions; PES sufficient to induce a tetanic motor contraction (30–50 Hz; strong motor intensity) and PES at sub motor-threshold intensity (100 Hz). PES applied to induce strong activation of APB increased the size of the N20-P25 component, thought to reflect sensory processing at cortical level, and increased corticomotor excitability. PES at sensory intensity decreased the size of the P25-N33 component and reduced corticomotor excitability. A positive correlation was observed between the changes in amplitude of the cortical SEP components and corticomotor excitability following sensory and motor PES. Sensory PES also increased the sub-cortical P14-N20 SEP component. These findings provide evidence that PES results in co-modulation of S1 and M1 excitability, possibly due to cortico-cortical projections between S1 and M1. This mechanism may underpin changes in corticomotor excitability in response to afferent input generated by PES. 相似文献
20.
After several unsuccessful management efforts, a split treatment of fluridone was applied to the 6700 ha Lake Harris in March and June 1987, at a rate of 3.4 kg ha–1 (680 and 340 kg fluridone, respectively) to two 3 m deep, hydrilla-infested bays. Fluridone concentrations in the water were sampled following the June treatment. Average fluridone concentrations were 2.1 µg l–1 prior to this second application, and a maximum concentration of 30.2 µg l–1 was detected in the treated area on the day following application. Fluridone residues dissipated out of the plot quickly due to dilution but concentrations declined lake-wide more slowly, following a logarithmic model, with an estimated fluridone half-life of 97 days. Control of hydrilla in Lake Harris resulted from the long exposure (over 25 weeks due to the split application) to fluridone concentrations of 2 µg l–1, well below the maximum labelled rate of 150 µg l–1. 相似文献