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1.
O. Zethner 《BioControl》1980,25(1):27-35
Agrotis segetum Schiff. granulosis virus (GV) propagated in Danish laboratory cultures was applied against field populations ofA. segetum in experimental latin square plots planted with beetroots, carrots and potatoes. Some test plots were isolated by net tents extending 15 cm into the soil whereas others were not caged. Plots were treated with suspensions of GV containing 106 to 108 capsules per ml with 50 ml being applied per m2 of plot. In 4 tests in which released eggs or larvae were caged over plots, cutworm numbers and damage were reduced by approximately 80% compared to untreated plots. The comparable reductions in 3 open experiments with natural populations of cutworms were 65–75%. GV-treatment 4 days after release of eggs appeared to be more effective than treatment 10 days after release. Whereas treatments with 107 and 108 capsules per ml reduced damages to approximately 75%, the effect of 106 Capsules was only 50%. Damage reduction by treatment with parathion varied from 50% in 2 caged experiments to approximately 20% in 2 open experiments, indicating that parathion was less effective than GV. The data indicated a residual effect of GV from one year to the next. Furthermore GV appeared to have spread at least 10 m from foci of application. 相似文献
2.
O. Zethner Bashir M. Khan M. Ismail Chaudhry B. Bolet Sharfuddin Khan Habibullah Khan Hanif Gul L. Øgaard M. Zaman Gul Nawaz 《BioControl》1987,32(5):449-455
Agrotis segetum Schiff granulosis virus (AsGV) propagated in Denmark was supplied against naturally occurring cutworm populations (A. ipsilon and to a less extentA. segetum) in experimental field plots of tobacco, okra, potato and sugar beet in northern Pakistan. AsGV doses varied between 4 ×
107 and 4 × 1011 capsules per m2 plot, and no. of applications between 1 and 3.
One treatment with AsGV did not reduce cutworm damage significantly to tobacco seedlings and potato plants.
Two treatments with AsGV reduced cutworm damage significantly. In tobacco, reduction was 64–82%, in okra and potato 85% and
77% respectively. Damage in sugar beet was reduced 78%.
Three treatments with AsGV dis not reduce damage significantly better than two treatments.
AsGV and the chemical insecticides Tamaran and Dieldrin, andBacillus thuringiensis (Thuricide) were about equally effective, reducing damage by 85%, 79%, 87% and 69%, respectively. No difference was found
between the efficiency of highly purified AsGV to which activated charcoal was added and partially purified AsGV without charcoal.
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3.
Laboratory studies showed that 1st-instarBonnetia comta (Fallén) maggots (planidia) had a significant impact (P<0.05) on mortality of all black cutworm (BCW),Agrotis ipsilon (Hufnagel), instars, either by killing 1st- and 2nd-instar BCWs 2.3 to 9.7 days after parasitization or by producing a puparium from older host instars. Diet consumption and utilization by BCW larvae parasitized byB. comta as 4th instars were similar to those of nonparasitized larvae until 1 to 2 days before the parasitoid emerged. In a 2-year host exposure study in Iowa, it was found thatB. comta primarily parasitized released BCWs in June through September and did not seem to play a role in controlling the damaging 1st generation of BCW larvae. Techniques were developed to produce and store large numbers ofB. comta planidia.Bonnetia comta deposited large numbers of planidia on filter paper treated with a fecal supernatant. These planidia could be stored on filter paper in a covered Petri dish at 4.4°C for 5 days with minimal mortality. Preliminary field data show that planidia placed around corn seedlings infested with 4th-instar BCW larvae do parasitize the pest and reduce the hosts cutting potential. 相似文献
4.
Microplitis mediator (Haliday) a gregarious endoparasite was recorded for the first time fromAgrotis segetum (Schiff) in Ankara, Turkey. The female parasites found their hosts by responding to the faeces of the caterpillars. An average,
females laid 15.5±1.6 eggs in the bodies of their hosts. The newly laid eggs were elongated, oval in shape and 0.23±0.004
mm long and 0.07 mm wide. They hatched in 5, 4 and 3 days at 20±2°C, 25±2°C and 30±2°C respectively when maintained at 60–70%
R.H. and 14∶10 light∶dark regime. At the same temperatures, the larval stage lasted for 24.9±0.6, 18.2±0.4 and 17.1±0.5 days
respectively. The prepupal stage was completed in 2 days at 25±2°C, whereas the prepupal and pupal (cocoon) stage lasted 10.9±0.2,
7.0±0.1 and 6.2±0.1 days respectively at the temperatures mentioned above. The adults started mating and feeding shortly after
emergence. Female parasites started laying after one day, 7–11 hours and 5–7 hours at the temperatures stated above. At these
temperatures females lived for 10.8±0.2, 5.4±0.1, 4.6±0.1 days and laid on average 556, 484 and 363 eggs respectively, whereas
the males survived 10.5±0.3, 4.7±0.1 and 4.4±0.1 days respectively.
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5.
A kairomone in the frass and vomitus of larvae ofAgrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae) triggered larviposition activity in its habitual parasitoidBonnetia comta (Fallen) (Diptera: Tachinidae). Laboratory bioassays showed that no measurable differences existed in the larviposition-stimulating activity of frass fromA. ipsilon larvae reared on 3 different food sources. In other tests, corn seedlings damaged by late-instar larvae ofA. ipsilon elicited strong larviposition activity inB. comta; other corn seedlings damaged with a razor blade did not elicit strong activity. Frass aged for 8-days was only slightly less effective at releasing a larviposition response when compared to fresh frass.B. comta was not stimulated to larviposit by oven dried frass or an India ink dot the color and shape of a fresh pellet from a host larva. The host habitat location and host finding process forB. comta and other tachinid species that deposit free-living maggots is discussed. 相似文献
6.
The ability of female larvae endoparasitoids [Microplitis croceipes Cresson:Cardiochiles nigriceps Viereck andCumpoletis sonorensis (Carlson)] to distinguish between unparasitized tobacco budworm,Heliothis virescens (F.), larvae andH. virescens larvae parasitized by the egg-larval parasitoidChelonus insularis,Cresson, was determined in laboratory studies. The 3 species of larval endoparasitoid females did not appear capable of distinguishing between unparasitized andC. insularis parasitized larvae resulting in multiple parasitoidism. The results of the ensuing competition between the 3 species for possession of the host demonstrated that bothC. sonorensis andM. croceipes were intrinsically superior toC. insularis. Both larva endoparasitoids destroyed the olderC. insularis larvae by physically attacking the latter. The presence ofC. insularis larvae in the host was found to prevent the hatch of compeatingC. nigriceps eggs through physiological suppression. The results show that the early attack of a host, as in the egg-larval parasitoid habit, is not necessarily advantageous. 相似文献
7.
Mortality responses ofTrichoplusia ni neonate larvae fed with the homologous granulosis virus are reported. The onset, duration and intensity of the associated
viremia are described. The prolonged eclipse phase of granulosis virus replication is compared with other baculoviruses.
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8.
Observations on the parasitoids of cotton bollworms in the Punjab were made during 1978 and 1979. The 2 trichogrammatid egg parasitoids, viz.Trichogramma achaeae Nagaraja & Nagarkatti andTrichogrammatoidea sp. nearguamensis Nagaraja (MS) were recovered fromPectinophora gossypiella (Saunders) in addition to 1 braconid egg-larval parasitoidChelonus sp. and 7 larval parasitoids, viz. 4 braconidsApanteles angaleti Muesebeck,Bracon greeni Ashsmead,Camptothlipsis sp. andRogas sp., 1 elasmid,Elasmus johnstoni Ferrière, 1 bethylid,Goniozus sp. and 1 ichneumonid,Scambus lineipes (Morley). FromEarias insulana Boisduval andEarias vittella F., 3 trichogrammatid egg parasitoids,T. achaeae, Trichogramma chilonis Ishii, andT. sp. nearguamensis 2 braconid larval parasitoids,B. greeni andRogas sp. and 1 chalcid pupal parasitoid,Brachymeria nephantidis Gahan, were recovered. One eurytomid hyperparasitoid,Eurytoma braconidis Ferrière was also recovered from the cocoons ofB. greeni. Of these parasitoids,T. achaeae, T. sp. nearguamensis, Camptothlipsis sp. andS. lineipes fromP. gossypiella, T. achaeae andB. nephantidis fromEarias spp. andE. braconidis fromB. greeni are new records. 相似文献
9.
Transmission of a nuclear polyhedrosis virus (NPV) inHeliothis zea (Boddie) on soybean was examinated. Artificial infestations ofH. zea were established at densities of 6.5, 19.5 and 58.5 larvae/row-m. Additional larvae infected to die from NPV in the 2nd stage
were released into subplots to simulate 5 and 25% mortality levels. Virus transmission from infected to noninfected larvae
was correlated with the initial incidence of infection in the population but not the density of larvae/row-m. Deposition of
virus on plants from cadavers of larvae that died of virus infection was correlated with the initial incidence of infection
in the populations and the density of larvae/row-m.
After pupation of larvae in the 1st population, noninfected larvae only were again released to examine transmission of viral
inoculum remaining on plants and soil. The percent mortality of larvae collected from the 2nd release was low and did not
differ significantly between treatments. The concentration of virus on foliage and in soil after the 2nd release was directly
correlated with density of larvae/row-m but not the incidence of infection within the population in the 1st release.
This material is based upon work supported in part by the U.S. Department of Agriculture under Agreement No 82 CRSR-2-1000. 相似文献
10.
A purified granulosis virus isolated fromPieris brassicae (L.) was tested in the field against an introduced population ofPieris rapae (L.) larvae on cabbage (cv January King) in small experimental plots at Littlehampton, Sussex. Experiments were designed to compare the relative efficacy of single and multiple applications of virus (2.1×1012 and 3.7×1012 or 2.1×1014 and 3.7×1014 virus capsules/ha) in reducing numbers ofP. rapae larvae. An experiment was carried out in June 1978 and repeated in August to coincide approximately with the 2 natural generations ofP. rapae in southern England. Larval populations were monitored by regularin situ assessment of plants and by destructive sampling. Within 10 days of spraying virus there was a significant reduction in the mean larval population on all virus-treated plots compared with untreated controls. Sprays of 2.1×1014 and 3.7×1014 capsules/ha reduced larval numbers more quickly than 2.1×1012 and 3.7×1012/ha treatments. In the 1st experiment, three sprays of virus at either 2.1×1012 or 2.1×1014 capsules/ha gave no increase over the final level of control achieved by a single spray. However, in the 2nd experiment, a single spray of 3.7×1012 capsules/ha did not significantly reduce the numbers of larvae. It is likely that this failure could be accounted for by a combination of the larger “natural” population ofP. rapae recorded midway through the 2nd experiment and the rapid inactivation of virus deposits which left little infectious virus to infect these larvae. Virus inactivation was so rapid that only 7–33 % of the initial virus deposits remained 1 day after application. These results suggest that further understanding of virus formulation, persistence and dosage rates are needed before such a virus can be used in a rational manner. 相似文献
11.
G. M. Tatchell 《BioControl》1981,26(3):291-299
The effects of temperature and granulosis virus infection both on the development ofPieris rapae L. and the area of cabbage leaf consumed by larvae were investigated. The duration of the larval period increased from 12.9
days at 24°C to 45.3 days at 13°C. The thermal constant for larval development was 196.1 day °C above the threshold temperature
of 9.4°C. The area of cabbage leaf consumed per day increased with larval age so that 5th instar larvae ate 70.9% of the total
food consumption. Virus infection extended the larval instar in which death occurred and reduced the potential food consumption
by as much as 99.6%. Larger virus doses reduced food consumption within the same instar more than smaller doses. The results
are discussed in relation to the feasibility of using granulosis virus for the control ofP. rapae.
Résumé L'étude a porté sur les effets de la température et de l'infection provoquée par le virus de la granulose sur le développement dePieris rapae L. et la quantité de feuillage de chou consommée par les larves. La durée du stade larvaire a augmenté de 12,9 j à 24°C à 45,3 j à 13°C. La constante thermique pour le développement larvaire a été de 196,1°C au-delà du seuil de température de 9,4°C. La superficie de feuillage consommée par jour s'est accrue en fonction de l'age des larves de sorte que les larves au 5e stade ont mangé 70,9% de la superficie totale consommée. L'infection provoquée par le virus a augmenté la durée du stade de mortalité et a diminué la consommation potentielle de feuillage de 99,6%. Des doses plus élevées de virus ont réduit la consommation dans le même stade davantage que les doses plus faibles. Les résultats sont discutés par rapport à la possibilité d'utiliser le virus de la granulose dans la lutte biologique contreP. rapae.相似文献
12.
The tobacco caterpillar,Spodoptera litura F. is a major pest in the tobacco nurseries causing damage by defoliation. Inundative releases were carried out with 650,000
parasites from 1st July, 1974 to 30th November, 1976. This paper reports the effectiveness from 1976 to 1979 of the parasite
in reducing the population of tobacco caterpillar.Telenomus remus
Nixon was found significantly effective in reducing the pest population at the peak of infestation in the site of release as compared
to control nurseries where no parasites were released. However, the difference inSpodoptera population between the site of release and control was minimal during the last 1979 nursery season which suggests the necessity
of inundative releases ofT. remus after every 2 years for effectively checking the population ofS. litura in tobacco nurseries.
Paper presented at the 4th Tobacco Symposium held at Rajahmundry-533 105, India, 19–22 Jan. 1981. 相似文献
Résumé La noctuelle du tabac,Spodoptera litura F. est un ravageur très important des pépinières de tabac du fait de ses défoliations. On a procédé à des lachers inondatifs de 650.000 parasites du 1er juillet 1874 au 30 novembre 1976. Cette note indique l'action du parasite entre 1976 et 1979 dans la réduction des populations de chenilles du tabac. On a constaté queTelenomus remus Nixon a une activité significative dans la réduction des populations du ravageur au moment du maximum d'infestation dans le lieu de lacher par rapport aux pépinières témoins sans introduction de parasites. Cependant la différence des niveaux de population entre le lieu de lacher et le terrain a été minimum lors de la dernière période de plantation en pépinière en 1979, ce qui suggère la nécessité d'effectuer tous les 2 ans des lachers inondatifs deT. remus pour lutter efficacement contreSpodoptera dans les pépinières de tabac.
Paper presented at the 4th Tobacco Symposium held at Rajahmundry-533 105, India, 19–22 Jan. 1981. 相似文献
13.
Pieris rapae (L.) an important pest of cole crops in the northeastern United States, is susceptible to a granulosis virus,Pieris rapae GV (PrGV), that has been shown to be an effective control measure by researchers in several countries. As an alternative
to weekly applications of virus to protect cabbage, we tested the use of an action threshold of one small (first-third instar)
larva per plant. Results were compared with those obtained using the same threshold with permethrin, and with weekly applications
of virus. Plots treated weekly with virus received 5 applications but the action threshold was exceeded only once. In all
virus-treated plots, numbers of large (fourth-fifth instar) larvae remained below 0.35 per plant, and were lower at the end
of the season (0.07 in plots treated weekly and 0.1 in plots treated once) than in either the untreated or permethrin-treated
plots (0.5). In late August, numbers of large larvae in the check plots reached almost 3 per plant. At harvest the number
of feeding holes over 0.3 cm in diameter in the 4 innermost frame and the 4 wrapper leaves were counted. Check plots differed
from treated plots by an average of 124.2±6.5 holes per plant in the frame and wrapper leave; virus-treated plots had 51.1±6.9
holes more than the permethrin plots. The difference in overall damage between plots treated 5 times with virus during the
season and those treated once was not significant. Plots treated once with virus had significantly more damage (7.6±2.7) to
wrapper leaves than those treated five times and marketability ratings were somewhat lower, based on fresh market standards.
There were no significant differences in head weight among the treatments. At harvest, a high proportion of larvae collected
from the check plots were diseased (77% versus an average of 46% in the treated plots). Because of the high numbers of large
larvae in the check plots in late August and the extensive damage to plants, we assumed that virus did not affect a significant
number of larvae in these plots until late in the growing season.
These results indicate the usefulness of PrGV in a cabbage IPM program and that the use of action thresholds can be highly
effective, particularly when insect numbers only occasionally reach damaging levels. While cabbage treated with permethrin
had the least amount of injury, that treated weekly with virus was not significantly different by fresh market standards,
and all cabbages treated with virus met processing standards. For the fresh market, in which cosmetic standards are more important,
PrGV may have to be used weekly or with an action threshold lower than one small larva per plant.
相似文献
14.
Three species of lepidopterous larvae were collected fromAmaranthus hybridus L. growing in field corn during 1975 and 1976 at Hastings, Florida.Spodoptera exigua (Hubner) was the predominant species in May.Spodoptera eridania (Cramer) was predominant in June andHerpetogramma bipunctalis (F.) in July and August. Nine native species of parasites, representing theBraconidae, Eulophidae, Ichneumonidae andTachinidae, emerged from these larvae.Meteorus autographae Muesebeck emerged from bothS. exigua andS. eridania. TheTachinidae, Winthemia rufopicta (Bigot),Eucelatori rubentis (Coquillett) andLespensia sp., emerged from mixtures ofS. exigua andS. eridania. Apanteles marginiventris (Cresson),Temelucha sp., andChelonus texanus Cresson emerged from bothS. exigua andH. bipunctalis larvae, andEuplectrus platyhypenae Howard andOphion sp. emerged fromS. eridania. All the species of parasites from the lepidopterous larvae that feed onAmaranthus hybridus are also reported as parasites ofS. frugiperda, a serious pest of corn. Therefore these larvae onA. hybridus may be a source of the parasites found attackingS. frugiperda. 相似文献
15.
Median lethal doses (LD50s) of nuclear polyhedrosis virus (NPV) were determined in neonatal offspring ofSpodoptera frugiperda (J. E. Smith) (Sf) larvae captured in southeastern Louisiana in 1981, 1982, and 1984. These LD50s ranged from 1.8 to 16.3 polyhedral inclusion bodies (PIB)/insect. The LD50s significantly (P<0.05) increased during the season of 1982 but had no pattern in 1981 or 1984. However, the Sf populations increased in heterogeneity of response to the NPV during all 3 years. The LD50 increased from 4.1 to 18.7 PIB/insect in a Sf laboratory colony exposed to the NPV LD80 for 7 generations, whereas in a control colony not exposed to NPV the LD50 was 5.9 PIB/insect after 7 generations. 相似文献
16.
Treatment ofPseudoplusia includens (Walker) larvae in either the 4th, 5th or 6th stage with a nuclear polyhedrosis virus (NPV) did not affect the weight or sex ratio of surviving pupae. Pupal mortality was low but increased with the dosage of the NPV larvae had received. Also, mortality of pupae from larvae treated in the 5th or 6th stage was higher than from larvae treated in the 4th stage. Adult longevity was not affected by the virus. Fecundity and egg viability were reduced following most treatments of 6th stage larvae but not 4th or 5th stage larvae. Adults exposed to NPV at 3 dosages during the 6th larval stage were released in cages on soybean. Some of the progeny succumbed to NPV infection. Progeny mortality increased over a 7 week period of adult release, but did not differ significantly between treated and untreated groups by the end of the 7 week period. 相似文献
17.
The relationships were surveyed among an introduced nuclear polyhedrosis virus (AgNPV), populations of its larval hostAnticarsia gemmatalis, and other invertebrates associated with soybean. Early applications of AgNPV to small plots effectively suppressedA. gemmatalis populations without causing detectable perturbations in populations of other phytophagous insects or invertebrate predators.
Bioassays of predators collected within and outside treated areas demonstrated that many invertebrates readily fed upon AgNPV-infectedA. gemmatalis under field conditions. These results suggest that the predator complex enhances the epidemic potential of this baculovirus.
The predators, by feeding upon virus infected larvae, are believed to play an important role in maintaining and disseminating
virus inoculum in soybean.
Florida Agricultural Experiment Station Journal Series No. 6272. 相似文献
18.
R. P. Jaques J. E. Laing C. R. MacLellan M. D. Proverbs K. H. Sanford R. Trottier 《BioControl》1981,26(2):111-117
The protection of apples against damage by the codling moth,Laspeyresia pomonella (L.), by applications of the granulosis virus ofL. pomonella was assessed in apple orchard tests at 4 locations in Canada in 1974–1978. Sprays containing 3×109 to 4×1010 granules/litre, applied 2 or 3 times per generation of codling moth larvae, reduced injury to apples by 44 to 85% compared
to reductions of 72 to 98% by applications of azinphos-methyl or phosmet. Applications of the virus did not reduce numbers
of arthropods predaceous on pest insects and mites.
Résumé L'efficacité de la protection des pommes contre le carpocapse,Laspeyresia pomonella (L.), par des applications du virus de la granulose deL. pomonella a été évaluée par des assais dans 4 vergers du Canada entre 1974 et 1978. Des pulvérisations contenant de 3×109 à 4×1010 granules/litre, appliquées 2 ou 3 fois par génération de larves du carpocapse des pommes, ont réduit les dommages aux pommes de 44 à 85% comparé à des réductions de 72 à 98% obtenues avec des pulvérisations d'azinphos-méthyl ou de phosmet. Les applications de virus n'ont pas réduit les populations d'arthropodes prédateurs d'insectes nuisibles et d'acariens.相似文献
19.
Resumen El desarrollo del bracónidoMeteorus rubens (Nees) se estudió en condiciones de laboratorio sobre las especies de noctuidosAgrotis ipsilon (Hufnagel),A. puta (Hübner),A. segetum (Denis & Schiffermüller),Peridroma saucia (Hübner),Spodoptera littoralis (Boisduval) yAutographa gamma (L.). Las larvas deA. ipsilon yA. puta fueron las únicas adecuadas para el desarrollo del parasitoide. EnA. ipsilon este bracónido parasitó y completó su desarrollo en el tercero, cuarto, quinto y sexto estadios larvarios, mostrando preferencia
por los dos últimos estadios. El número de hembras que parasitan, el porcentaje de larvas parasitadas y el número de parasitoides
emergidos por hospedante estuvo en relación directa con la edad del hospedante en el momento de la parasitación. En las larvas
parasitadas en el tercer estadio el tiempo de desarrollo del parasitoide fue significativamente mayor que en los otros tres
estadios. Sin embargo, el estadio en que fueron parasitadas las larvas no influyó en la longevidad de los adultos.
相似文献
20.
A survey forPrays acmonias
Meyr. and its natural enemies was made in the northern hills in Pakistan. It attackedViburnum spp. in the Murree hills, Azad Kashmir and Swat. Its populations were generally low at most localities.Deraecoris sp.,Haplothrips sp. (egg predators),Apanteles sp. nr.dilectus
Hal.,Elasmus hyblaeae
Ferrière,Elachertus sp.,Euderus sp. andSympiesis sp. (larval parasites),Sphedanolestes sp. (larval predator),Aspergillus flavus
Link andStreptococcus sp. (pathogens) attackedP. acmonias. Among theseApanteles was more common throughout the distribution area of the host and destroyed up to 13% host larvae. It appears to be a possibly
promising parasite for trials in areas wherePrays spp. are pests. However, it did not parasitizePrays oleae (Bern.).
This work was sponsored by the Food and Agriculture Organisation of the United Nations as a part of the project entitled “Research on the control of olive pests and diseases in continental Greece, Crete and Corfu”. 相似文献
Résumé Cette chenille qui attaque lesViburnum spp. dans les collines du Nord du Pakistan s'y trouve généralement en faibles densités.Deraecoris sp.,Haplothrips sp. (prédateurs des œufs),Apanteles sp. nr.dilectus Hal.,Elaemus hyblaeae Ferrière,Elachertus sp.,Euderus sp. etSympiesis sp. (parasites des larves),Sphedanolestes sp. (prédateur des larves),Aspergillus flavus Link etStreptococcus sp. (germes pathogènes) sont les ennemis naturels deP. acmonias. Le plus commun estApanteles, qui peut détruire jusqu'à 13% des chenilles. Il peut être un parasite intéressant pour la lutte contre desPrays spp., mais il ne parasite pasPrays oleae (Perm.).
This work was sponsored by the Food and Agriculture Organisation of the United Nations as a part of the project entitled “Research on the control of olive pests and diseases in continental Greece, Crete and Corfu”. 相似文献