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1.
An investigation has been made into the growth regulators presentin ethanol extracts of the seedling roots of Vicia faba afterseparation on paper partition chromatograms, using segmentsof Avena coleoptiles and mesocotyls and of Pisum sativum.rootsas biological assay material. Acetonitrile purification shows the presence of at least threeauxins running in isobutanol: methanol: water, at Rfs of 00·25,0·40·6, and 0·650·95;the latter may represent two different auxins. A similar, butclearer, picture is shown by the ether-soluble acid fraction.Here an auxin at Rf 00·25 also stimulates rootgrowth and could be accelerator . A second atRf 00·25 is an indole compound which inhibitsroot growth and does not seem to be be IAA. A third at Rf 0·81·0is also a root-growth inhibitor and gives no indole reaction.The inhibitor ß complex was demonstrated(Rf 0·650·85) together with a number ofother inhibitors at lower Rf value. The ether-soluble neutral component also contains auxins orauxin precursors. The water-soluble, ether-insoluble fraction contains four readilyinterconvertible substances with auxin properties. They allappear to inhibit root growth and give no indole reaction. 相似文献
2.
Extracrts of the shoot tips of normal and frenchedtobacco plants were chemically separated into acidic, neutral,and basic ethersoluble fractions. On chromatograms ofthese, some plant growth regulators were assayed using the Avenacoleoptile section extension test. The acidic auxins and an acids and a neutral growth inhibitorwere found. One auxin, with the samew RF value as indole-3-aceticacid, was four times more concentrated on normal as in frenchedplants. No differences could be established between the twotypes of plants in regard to other growth regulators detected. It is argued that the symptoms of the physiological diseasefrenching could be explained in terms of a auxindeficiency. 相似文献
3.
Developmental control of xylem hydraulic resistances and vulnerability to embolism in Fraxinus excelsior L.: impacts on water relations 总被引:3,自引:0,他引:3
Welbaum G.E.; Bian D.; Hill D.R.; Grayson R.L.; Gunatilaka M.K. 《Journal of experimental botany》1997,48(3):655-663
The freezing tolerance of many plants, such as pea (Pisum sativum),is increased by exposure to low temperature or abscisic acidtreatment, although the physiological basis of this phenomenonis poorly understood. The freezing tolerance of pea shoot tips,root tips, and epicotyl tissue was tested after cold acclimationat 2C, dehydration/rehydration, applications of 104M abscisic acid (ABA), and deacclimation at 25C. Tests wereconducted using the cultivar Alaska, an ABA-deficientmutant wil, and its wildtype. Freezinginjury was determined graphically as the temperature that caused50% injury (T50) from electrical conductivity. Endogenous ABAwas measured using an indirect enzyme-linked immunosorbant assay,and novel proteins were detected using 2-dimensional polyacrylamidegel electrophoresis. The maximum decrease in T50 for root tissuewas 1C for all genotypes, regardless of treatment. For Alaskashoot tips and epicotyl tissue, exogenous ABA increased thefreezing tolerance by 1.5 to 4.0C, while coldtreatment increased the freezing tolerance by 7.5 to14.8C. Cold treatment increased the freezing toleranceof shoot tips by 9 and 15C for wiland wild-type, respectively. Cold acclimationincreased endogenous ABA concentrations in Alaskashoot tips and epicotyls 3- to 4-fold. Immunogold labeling increasednoticeably in the nucleus and cytoplasm of the epicotyl after7 d at 2C and was greatest after 30 d at the time of maximumfreezing tolerance and soluble ABA concentration. Cold treatmentinduced the production of seven, three, and two proteins inshoot, epicotyl, and root tissue of Alaska, respectively.In Alaska shoot tissue, five out of seven novelproteins accumulated in response to both ABA and cold treatment.However, only a 24 kDa protein was produced in wiland wild-type shoot and epicotyl tissues aftercold treatment. Abscisic acid and cold treatment additivelyincreased the freezing tolerance of pea epicotyl and shoot tissuesthrough apparently independent mechanisms that both resultedin the production of a 24 kDa protein. Key words: Pisum sativum, cold acclimation, immuno-localization 相似文献
4.
Welbaum G.E.; Bian D.; Hill D.R.; Grayson R.L.; Gunatilaka M.K. 《Journal of experimental botany》1997,48(3):643-654
The freezing tolerance of many plants, such as pea (Pisum sativum),is increased by exposure to low temperature or abscisic acidtreatment, although the physiological basis of this phenomenonis poorly understood. The freezing tolerance of pea shoot tips,root tips, and epicotyl tissue was tested after cold acclimationat 2C, dehydration/rehydration, applications of 104M abscisic acid (ABA), and deacclimation at 25C. Tests wereconducted using the cultivar Alaska, an ABA-deficientmutant wil, and its wildtype. Freezinginjury was determined graphically as the temperature that caused50% injury (T50) from electrical conductivity. Endogenous ABAwas measured using an indirect enzyme-linked immunosorbant assay,and novel proteins were detected using 2-dimensional polyacrylamidegel electrophoresis. The maximum decrease in T50 for root tissuewas 1C for all genotypes, regardless of treatment. For Alaskashoot tips and epicotyl tissue, exogenous ABA increased thefreezing tolerance by 1.5 to 4.0C, while coldtreatment increased the freezing tolerance by 7.5 to14.8C. Cold treatment increased the freezing toleranceof shoot tips by 9 and 15C for wiland wild-type, respectively. Cold acclimationincreased endogenous ABA concentrations in Alaskashoot tips and epicotyls 3- to 4-fold. Immunogold labeling increasednoticeably in the nucleus and cytoplasm of the epicotyl after7 d at 2C and was greatest after 30 d at the time of maximumfreezing tolerance and soluble ABA concentration. Cold treatmentinduced the production of seven, three, and two proteins inshoot, epicotyl, and root tissue of Alaska, respectively.In Alaska shoot tissue, five out of seven novelproteins accumulated in response to both ABA and cold treatment.However, only a 24 kDa protein was produced in wiland wild-type shoot and epicotyl tissues aftercold treatment. Abscisic acid and cold treatment additivelyincreased the freezing tolerance of pea epicotyl and shoot tissuesthrough apparently independent mechanisms that both resultedin the production of a 24 kDa protein. Key words: Pisum sativum, cold acclimation, immuno-localization 相似文献
5.
Previous experiments on the effects of auxins on the geotropicresponses of seedling pea roots (Audus and Brownbridge, 1957)have been extended using the anti-auxin -(I-naphthylmethylsulphide)propionicacid (NMSP) alone and in combination with indole-3-acetic acid(IAA) and 2:4-dichlorophenoxyacetic acid (2:4-D). NMSP action differs from that of the auxins in that it reducesthe rate of curvature progressively as the concentration isincreased, irrespective of whether the overall extension growthof the roots is being stimulated (10 and 30 p.p.m.) or inhibited(100 p.p.m.). Correspondingly the reaction time is lengthenedby 2550 per cent. in all concentrations. Studies of responsesin mixtures of growth-stimulating concentrations of NMSP (30p.p.m.) and growth-inhibiting concentrations of IAA (108)and 2:4-D (3 x 108) show that auxins and antiauxinsare mutually antagonistic in most, if not all, their actionson growth and curvature. The results suggest that the anti-auxin NMSP may stimulate rootgrowth and inhibit curvature by interfering with the synthesisor distribution of a natural endogenous inhibitor, which isnot IAA. NMSP inhibition of root growth in high concentrationsmust, however, be exerted independently of this natural inhibitor.The mutual antagonisms shown between the auxins and NMSP arebest explained in terms of an interference with access to thegrowth centres; competitive action at the growth centres themselvesseems not to be involved. 相似文献
6.
Ethylene Production by Fe-deficient Roots and its Involvement in the Regulation of Fe-deficiency Stress Responses by Strategy I Plants 总被引:5,自引:1,他引:5
Species that showed marked morphological and physiological responsesby their roots to Fe-deficiency (Strategy I plants) were comparedwith others that do not exhibit these responses (Strategy IIplants). Roots from Fe-deficient cucumber (Cucumis sativusL.Ashley), tomato (Lycopersicon esculentumMill.T3238FER) and pea (Pisum sativumL. Sparkle) plantsproduced more ethylene than those of Fe-sufficient plants. Thehigher production of ethylene in Fe-deficient cucumber and peaplants occurred before Fe-deficient plants showed chlorosissymptoms and was parallel to the occurrence of Fe-deficiencystress responses. The addition of either the ethylene precursorACC, 1-aminocyclopropane-1-carboxylic acid, or the ethylenereleasing substance, Ethephon, to several Fe-sufficient StrategyI plants [cucumber, tomato, pea, sugar beet (Beta vulgarisL.),Arabidopsis(Arabidopsis thaliana(L.) Heynh Columbia), plantago(Plantago lanceolataL.)] promoted some of their Fe-deficiencystress responses: enhanced root ferric-reducing capacity andswollen root tips. By contrast, Fe-deficient roots from severalStrategy II plants [maize (Zea maysL. Funo), wheat(Triticum aestivumL. Yécora), barley (HordeumvulgareL. Barbarrosa)] did not produce more ethylenethan the Fe-sufficient ones. Furthermore, ACC had no effecton the reducing capacity of these Strategy II plants and, exceptin barley, did not promote swelling of root tips. In conclusion,results suggest that ethylene is involved in the regulationof Fe-deficiency stress responses by Strategy I plants.Copyright1999 Annals of Botany Company. Arabidopsis (Arabidopsis thaliana(L.) Heynch), barley (Hordeum vulgareL.), cucumber (Cucumis sativusL.), ethylene, iron deficiency, maize (Zea maysL.), pea (Pisum sativumL.), plantago (Plantago lanceolataL.), ferric-reducing capacity, sugar beet (Beta vulgarisL.), tomato (Lycopersicon esculentumMill.), wheat (Triticum aestivumL.). 相似文献
7.
Increased Synthesis of ABA in Partially Dehydrated Root Tips and ABA Transport from Roots to Leaves 总被引:27,自引:3,他引:24
Zhang, J. and Davies, W. J. 1987. Increased synthesis of ABAin partially dehydrated root tips and ABA transport from rootsto leaves.J. exp. Bot. 38: 20152023. Isolated root tips of pea (Pisum sativum L. cv. Feltham First)and Commelina communis L. were air-dried until they lost between10% and 40% of their fresh weight, followed by a period of incubationat these reduced water contents. These treatments resulted inincreased ABA production, suggesting that root tips of bothspecies have the capacity to synthesize ABA in increased amountswhen water deficits develop in the root. The ABA concentrationin pea roots increased linearly as turgors fell below about0·15 M Pa and relative water contents (R WC) fell below90%. Commelina roots produced more ABA when RWC fell below asimilar value but the threshold turgor for increased ABA productionin Commelina roots was around 0·30 MPa. Roots of intact plants loaded with ABA as a result of incubationin solutions of varying concentrations provided ABA to leaveswhich resulted in increased ABA concentrations in the leaveswhen these were assayed several hours later. This occurred whenthese roots were not contributing substantially to transpirationalflux. Leaves on shoots that were enclosed and darkened and thereforenot transpiring, did not accumulate ABA from loadedroots. A role for root-sourced ABA in root-to-shoot communication ofthe effects of soil drying is discussed. Key words: ABA, roots, water relations 相似文献
8.
Some Constituents of Xylem Sap and their Possible Relationship to Xylem Differentiation 总被引:1,自引:1,他引:0
Bleeding sap of Actinidia chinensis and Betula populifolia andguttation fluidof Avena sativa were analysed for sugars, amino-acids,auxin, and certain enzymes. A wide range of amino-acids wasfound in all three. Auxin was not detected in the bleeding sap,but was present in Avena guttation fluid (5.1 µg IAA equivalent/l).IAA oxidase, acid phosphatase, ribonuclease, deoxyribonuclease,and protease were detected in the bleeding sap and guttationfluid. The possibility that some of the substances found insap and guttation fluid are products of autolysing, differentiatingxylem cells in the roots is discussed. 相似文献
9.
《Journal of experimental botany》1977,28(6):1247
Effects of coupled solute and water flow in plant roots withspecial reference to Brouwer's experiment. Edwin L. Fiscus. p. 71 Abstract: Line 3 delete interval insertinternal. p. 73 Materials and Methods: line 6: delete diversion insert division line 9 equation should read Jv=Lp PRT(C0C1). 74 Last line of figure legend: 101 should read 1011. 75 Line 11: delete seems insert seem. le 1 column heading106 should read 1011. 77 delete ...membrane in series of... insert membranein series or... Delete final paragraph. 相似文献
10.
The present study investigates the effects of triacontanol (CH3(CH2)28CH2OH),on plant growth (root and stem), peroxidase activity (apicalmeristem tissue), and auxin destruction (apical meristem tissue)in Little Marvel dwarf (LM) and Alaskapeas (AP). Triacontanol inhibited root growth in LM comparedto untreated controls. However, root growth in AP tissue wasenhanced by 1.0 mg I1 triacontanol and inhibited by allother treatments, in comparison to untreated controls. Wateruptake in triacontanol-treated AP plants was greater than inuntreated controls, with the converse being the case for LM.Triacontanol treatment caused an increase in peroxidase activityin both LM and AP plants compared to untreated controls. Interms of (114C)IAA destruction, GA3 + 0.01 mg 11triacontanol caused appreciable auxin breakdown (40%) in LMtissue, with GA3 + 0.1 mg 11 triacontanol giving a 43%decrease compared to untreated controls. In AP tissue, 10 µMGA3 increased auxin destruction by 188% whereas 0.1 mg I1triacontanol caused a 20% decrease compared to untreated controls.The effects of triacontanol on root and stem growth, peroxidaseactivity, and auxin destruction appear to be cultivar-specific,with respect to LM and AP varieties of peas. 相似文献
11.
Cultured shoot tips of Dianthus caryophyllus often develop asabnormal, vitrified plants that have the characteristicsof teratomata: stable, unlimited development with fasciatedshoot apices, a bush habit, reduced stem elongation and succulentleaves. High concentrations of NAA in the culture medium increasedand benzylaminopurine decreased the proportion of shoot-tipsthat developed as abnormal plants. The requirements of abnormaland normal plants for continued development differed. Duringthe initial stages of development abnormal plants did not requireauxin and they were able to continue their growth in the absenceof roots. It is suggested that the abnormal plants are teratomata,similar to those induced by Crown Gall bacteria, and their inductionand abnormal traits could be due to imbalance of auxins andcytokinins. Dianthus caryophyllus L., carnation, abnormal plant growth, habituation, vitrification, teratoma (plant) 相似文献
12.
The Geoelectric Effect in Plant Shoots: III. DEPENDENCE UPON AUXIN CONCENTRATION GRADIENTS AND AEROBIC METABOLISM 总被引:1,自引:0,他引:1
The development of the geoelectric effect has been followedin Zea coleoptiles with a flowing-solution electrode system,and its dependence upon auxin concentration gradients and aerobicmetabolism assessed. A symmetrical source of IAA can effectively replace the coleoptiletip in allowing the geo-electric potential to occur. The diffusatefrom coleoptile tips, when applied asymmetrically to the apexof a vertical decapitated coleoptile, generates a potentialdifference across the coleoptile indistinguishable from thatinduced by the asymmetrical application of IAA. Asymmetricalapplication of IAA to vertical Avena and Zea coleoptiles andHelianthus hypocotyls induces closely similar responses. Neither the geoelectric effect nor a geotropic response developswhen intact Zea coleoptiles are placed horizontally after beingdeprived of oxygen, but they both occur when an aerobic atmosphereis restored. The lateral potential difference induced by theasymmetrical application of IAA to the apex of a vertical coleoptiledoes not occur under anoxic conditions. With a static-drop electrode system and a decapitated Zea coleoptile,a potential difference develops immediately after reorientationof the coleoptile into the horizontal position, and attainsa maximum value after about 10 min. This potential differencecan be further increased by the asymmetrical application ofIAA to the lower half of the apical cut surface of the coleoptile. Our data support the view that both the geoelectric potentialand the geotropic response are due to the IAA concentrationgradient which arises from the lateral transport of this substancefrom the upper to the lower half of the horizontal shoot. Theyalso bear out our previous conclusions that the geoelectricpotential observed with static-drop electrodes and anintact shoot, is the resultant of two processes. The first isa physical phenomenon arising in the electrodes, or betweenthe electrodes and the plant tissue, and the second arises inthe living tissues of the shoot as the result of gravity-inducedchanges in auxin distribution. 相似文献
13.
Extracts of small and mature-size lupin pods yielded four substancesaffecting the growth of wheat-coleoptile sections: one acidpromotor (A), two acid inhibitors(B and X), and one neutralinhibitor(Y). Inhibitor B was extremely active, however, coleoptile sectionsshowed no signs of toxic effects; they resumed growth at a rapidrate after rinsing them and adding ß-indolylaceticand (IAA) to the medium. 1 µg of IAA was required to counteractthe effect of B extracted from 230 mg. Of tissue.On an equal fresh weight basis the inhibiting action of Bin lupin pods was 5001,500 times more potent than thatof inhibitor ß in etiolated pea seedlings. Small pods of plants infected with pea-mosaic virus yielded3 times the amount of A of healthy plants (equivalentto 1 µg. IAA 0.3 µg. IAA per 25 g. of tissue respectively),and approximately the amount of B. Mature podsof virus-infected plants again yielded moreA,but also 2? times more B than pods of healthyplants. Healthy pods yielded more A than virus-infectedpods, and there was no difference in X. A lupin abscission test was developed and the effects of proximaland distal application of -naphthyl acetic acid (NAA) are presented,and discussed with respect to results of other abscission tests. A accelerated abscission when applied proximally,and delayed or prevented it when applied distally. Bstrongly accelerated abscission when applied in either way.A possible mechanism explaining the abscission-inducing effectof developing pods on later flowers is discussed in terms ofthe substances A and B. The partlyprevented abscission observed on virus-infected plants was foundto agree well with the proposed mechanism. 相似文献
14.
The Nitrogen Content of Plants and the Self-thinning Rule of Plant Ecology: A Test of the Core-skin Hypothesis 总被引:3,自引:0,他引:3
The core-skin hypothesis postulates that secondarilythickened plants behave energetically as an inert corecovered by an active skin, the skinbeing two-imensional, the core three-dimensional.This would explain the self-thinning or3/2 rule of plant ecology, that is, the tendencyfor log (dry weight per plant) and log (number of plants perunit area) to progress along a straight line relationship, withslope = 3/2. The hypothesis was tested as follows. Plant nitrogen contentwas used as an estimate of the mass of skin perplant, and dry weight as an estimate of the mass of the core.As plants mature the slope of the relationship between y = log(mass of nitrogen per plant) and x = log (mass of dry matterper plant) is expected to decline from an initial value of 1.0towards a final value of 0.66. The intercept of the relationshipis expected to reflect the intrinsic content of skinper unit of core. Genotypic variation in thisparameter should cause genotypic differences in the maximumattainable yield of biomass per unit area. The expectations were investigated by fitting the function y= p+qx+r exp x to 30 sets of data on plant nitrogencontent, plant weight and time in 18 different vegetables. Simplelinear regressions of y on x were fitted to more limited setsof data on weights and nitrogen contents of mature trees. Theexpectations were, with some minor exceptions, confirmed. Nitrogen, yield, plant competition, self-thinning 相似文献
15.
Mode of action of FC was compared with that of auxin in differentexperimental systems and the following results were obtained.
- FC, as well as auxin, primarily induced elongation of the epidermisof pea epicotyl segments, but it also promoted elongation ofthe inner tissue, as judged by its action in split stem tests,elongation of hollow-cylinder segments and elongation of unpeeledand peeled segments.
- FC decreased the minimum stress relaxationtime (T0) and increasedthe extensibility (mm/gr) of the epidermalcell wall of peaepicotyl segments, as did auxin.
- FC failedto induce expansion growth of Jerusalem artichoketuber sliceswhen given alone or in combination with kinetinor gibberellicacid.
- FC at concentrations lower than 106 M, when givenwithauxin at concentrations lower than 0.03 mg/liter, promotedelongationof Avena coleoptile segments in an additive manner,to achievethe maximum elongation at higher concentrations.
- An antiauxin, 2,4,6-trichlorophenoxyacetic acid, inhibitedtheelongation of Avena coleoptile segments due to auxin butnotthat due to FC.
- Nojirimycin, an inhibitor of ß-glycosidases,inhibitedelongation of pea internode segments due not onlyto auxin butalso to FC.
- At concentrations more than 105MFC promoted root elongationof intact lettuce seedlings, whichwas inhibited by exogenousauxin.
16.
The temperature dependence of the efflux kinetics of labelledwater in isolated maize roots has been studied. The purposeof these experiments was to obtain the energy of activation,E (kcal/mole), of the rate-limiting step in this radial exchangeprocess under various experimental conditions. Estimates ofE were obtained from linear relations between ln{D'w} and thereciprocal of the absolute temperature; values of the apparentdiffusion coefficient, D'w, of labelled water in the root werefound from an analytical treatment of the efflux data in termsof a cylindrical diffusion model. The energy of activation forlabelled-water exchange in normal roots was 14.9 kcal/mole.The corresponding value for dead (boiled) rootswas 3.9 kcal/mole. These values of E substantiate the view thatin normal roots the penetration of water across the membranesof the root cells constitutes the rate-determining step in theefflux whereas in dead roots extracellular diffusionof water is the source of rate-control. Similar temperature dependence studies were performed on theefflux kinetics from normal and dead roots treatedwith 105 M phenylmercuric acetate (PMA). The energiesof activation for labelled-water exchange in normal and deadroots under these conditions were respectively 15.5 and 5.3kcal/mole. Moreover, the results of the efflux experiments onPMA-treated roots were considered to indicate that this inhibitorproduces an alteration in some structural aspect of the rate-controllingmembranes. 相似文献
17.
It has been widely accepted for over 50 years that the elongationrate of a coleoptile is dependent on the supply of auxin fromthe apex. The original coleoptile decapitation experiments whichprovided support for this view have been repeated but the measurementsof coleoptile elongation were made with greater temporal andspatial precision. The experiments confirm that Avena and Zeacoleoptile elongation is retarded by decapitation but the locationand timing of the growth rate changes are not consistent withthe hypothesis that decapitation reduces growth rate solelyby removing the major supply of auxin. Evidence is presentedthat wounding is the prime cause of the effects of decapitation.Data are also presented showing that the recovery of growthrate of coleoptiles after decapitation or wounding is not dearlyassociated with any events near the cut surface and hence thetraditional explanation of this phenomenon (regenerationof the physiological tip) is misleading. Key words: Coleoptile, decapitation, apex 相似文献
18.
《Journal of Molluscan Studies》1953,29(6):258
Part 1, under the frontispiece portrait of Dr. N. B. Eales,the words President 19481951 should havebeen added. Page 103, line 49, for Newton Collection readNorman Collection (Canon Norman). 185, line 37, for capillaris read capillacca. 188, Table 1, for bemoralis. read nemoralis. 188, Table 2, for Cochlicella acuta (Müll)? ventrosa(Fér.) read Cochlicella ventrosa (Fér.). 191, line 24, for araheo- read archeo-. 相似文献
19.
The relationship between the induction of tracheary elementdifferentiation and exogenous L-methionine was examined in agar-growncultures of soya bean callus initiated from Glycine max L. Wayneand Clark 63. Although Wayne is a normal cultivarsoya bean, seedlings of Clark 63 exhibit abnormal growth at25 °C due to exessive ethylene biosynthesis at this temperature.Wayne callus showed increased xylogenesis in the presence ofexogenous L-methionine (3.7 µg 11) in comparisonto IAAKN controls at both 20 and 25 °C. Clark 63callus produced greater numbers of tracheary elements in responseto exogenous L-methionine only at 25 °C. The induction ofxylem differentiation was independent of the maintenance temperatureof the stock cultures of both cultivars. Xylogenesis initiatedbyan IAAKN medium was inhibited by the addition of AgNO3(20 mg 11) to the extent of 76.5 per cent in cv. Wayneand 6 per cent in cv. Clark 63. The inhibitory effect was partiallyreversed by the addition of L-methionine (3.7 µg 11)to the IAAKNAgNO2 medium. These data support thehypothesis that xylogenesis in vitro involves auxin, cytokininand ethylene. differentiation, xylogenesis, L-methionine, ethylene, Glycine max L., soya bean, callus culture, auxin, kinetin 相似文献
20.
DRAKEFORD DAVID R.; MUKHERJEE ILABANTA; REID DAVID M. 《Journal of experimental botany》1985,36(11):1705-1715
Drakeford, D. R., Mukherjee, I. and Reid, D. M. 1985. Some earlyresponses of Helianthus annuus L. to flooding. I. The effectsof flooding on the uptake and leakage of non-electrolytesby roots.J. exp. Bot. 36: 17051715. The object of this work was to examine some of the early effectsof flooding on roots. A hydroponic system was developed thatgave good control over watering, degree of oxygenation of thebathing medium and allowed measurement of short term changesin the composition of the bathing medium. Excised roots, floodedfor 24 h, were shown to take up less [3H) ß-alaninethan non-flooded roots and also leaked more [3H] ß-alanineinto a distilled water bathing medium. Further, flooded excisedroots lost more protein to the bathing medium, with young(57 d) roots showing greater losses than old(1114 d) roots. However, young roots had more proteinin the tissue even after greater loss. Young roots remainedhealthier and lost less fresh weight than old roots. Abscisicacid was shown to have a small role in protecting youngroots from the effects of flooding. Key words: ABA, abscisic acid, anaerobic, flooding, leakage, roots, uptake, waterlogging 相似文献