Experimental measurements and design guidelines for real-time software encryption in multimedia wireless LANs

To secure interactive multimedia applications in wireless LANs (WLANs), it is pertinent to implement real time cryptographic services. In this paper we evaluate the use of software based encryption algorithms that are implemented in the layer service provider as defined by WinSock 2 for Windows 95/NT. Our measurements show that software implementation of various encryptors can sustain the throughput requirements of interactive multimedia applications for WLANs such as telephone-quality audio, video conferencing, and MPEG video. We present a design methodology that includes guidelines for a secure multimedia system design in terms of the encryption method chosen as a function of required application throughput, system configuration, protocol layers overhead and wireless LAN throughput. This revised version was published online in July 2006 with corrections to the Cover Date.     

QoS Support by the HiperLAN/2 MAC Protocol: A Performance Evaluation

HiperLAN/2 (HIgh PErformance Radio Local Area Network) is a new standard from ETSI (European Telecommunications Standards Institute) for high-speed wireless LANs, interconnecting portable devices to each other and to broadband core networks, based on different networking technologies such as IP, ATM, IEEE 1394, and others. This paper introduces the basic features of the HiperLAN/2 MAC protocol. It presents performance evaluation results, specifically related to the mechanisms provided by HiperLAN/2 to manage bandwidth resource requests and granting. These results are assessed in terms of their flexibility and efficiency in supporting delay sensitive traffic, such as voice and Web data traffic, which are expected to be transported by broadband wireless LANs.     

Effects of a wireless local area network (LAN) system, a telemetry system, and electrosurgical devices on medical devices in a hospital environment

Concerns have been raised about interference of wireless local area network (LAN) systems and telemetry systems with medical devices in hospitals. The authors have investigated the susceptibility of 65 electromedical devices to a wireless LAN system and a telemetry system in preselected areas of a hospital. Testing was based on the American National Standards Institute Standard C63.18. The wireless LAN system operated at 2.42 GHz with an output power of 100 mW. The telemetry system operated at 466 MHz with an output power of 4 mW. Of the 65 devices tested, only two hand-held Doppler units, a Mini Doppler Model D900 (Huntleigh Healthcare Ltd) and a Ultrasonic Doppler Model 811 (Parks Medical Electronics, Inc.), were affected by the LAN system. Placed within 10 cm of the LAN system in standby mode, both units emitted periodic high-pitched beating sounds, which could be misinterpreted as normal beating sounds from the patient. These changed to random static noise during data transmission by the LAN. Under normal conditions of use, a LAN system would never be placed this close to a medical device. The quality of data transmission from the LAN system changed from "good" to "acceptable" in the colonoscopy room. This deterioration in transmission quality could have been caused by the lead shielding in the room. Electrosurgical devices operating at 0.5 to 1 MHz did not affect the LAN system at distances up to 3 m. None of the devices was affected by the telemetry system. These findings suggest that wireless LAN systems and telemetry systems can be acceptable for use in hospitals. Nevertheless, other systems should be tested on potentially susceptible devices by the hospital before use.     

Collision avoidance and resolution multiple access (CARMA)

The collision avoidance and resolution multiple access (CARMA) protocol is presented and analyzed. CARMA uses a collision avoidance handshake in which the sender and receiver exchange a request to send (RTS) and a clear to send (CTS) before the sender transmits any data. CARMA is based on carrier sensing, together with collision resolution based on a deterministic tree-splitting algorithm. For analytical purposes, an upper bound is derived for the average number of steps required to resolve collisions of RTSs using the tree-splitting algorithm. This bound is then applied to the computation of the average channel utilization in a fully connected network with a large number of stations. Under light-load conditions, CARMA achieves the same average throughput as multiple access protocols based on RTS/CTS exchange and carrier sensing. It is also shown that, as the arrival rate of RTSs increases, the throughput achieved by CARMA is close to the maximum throughput that any protocol based on collision avoidance (i.e., RTS/CTS exchange) can achieve if the control packets used to acquire the floor are much smaller than the data packet trains sent by the stations. Simulation results validate the simplifying approximations made in the analytical model. Our analysis results indicate that collision resolution makes floor acquisition multiple access much more effective. This revised version was published online in August 2006 with corrections to the Cover Date.     

Scrutiny of unruly and abuse in wireless networks to mitigate physical layer threats using discriminate based misbehavior prevention

The open nature of communication medium in wireless networks becomes easy for the attackers to indulge in jamming attack. Jamming blocks the communication channel with the intent of preventing the flow of useful information. Jammers effectively and stealthy corrupts the packet by injecting high level of noise thereby keeping the channel busy so that the legitimate traffic gets completely blocked, resulting in packet loss at the receiver side. Securing the information from the jammers has become very important. Therefore an effective approach is needed to prevent this attack and this paper proposes Discriminate based Misbehavior Prevention DMP scheme to identify and to detach the jammer that corrupts the packet in wireless network. The proposed scheme consists of three modules. In module one, the log files are analyzed and using the trusting mechanism the suspected traces are identified. In module two, three reshuffling algorithms has been developed for reshuffling and identifying doubtful traces. In module three, the jammer nodes are identified and detached from the network. By simulation studies, it is observed that the proposed scheme attains higher throughput and packet delivery ratio while attaining lower delay.     

Histological analysis of the aortic nerve in the rat raised in space (Rapid communication on Neurolab Project).

To study development of the aortic nerve baroreflex under conditions of microgravity, we examined the cross section of the left aortic nerve (LAN), which is the afferent of the baroreflex, in the neonate rats aged 25 days raised in microgravity on the space shuttle Columbia (flight:FLT group) for 16 days. In this paper, we report a part of the result obtained from the data of the myelinated fibers of LAN analyzed with an electron microscope. Two kind of ground control groups were compared to the FLT group; one was asynchronous ground control (AGC) group where the rats were housed in the same cage as that on the shuttle, and the other was vivarium(VIV) group where the rats were housed in a commercial cage. The LANs in each group were extirpated the from rats perfused with a fixative and embedded for histological analysis. We observed the transverse sections of LAN and took pictures of several areas (magnified to x 2K to x 200K). No irregular myelination was found in all fibers of FLT group when they were compared with two control groups. The thickness of myelin of the maximally myelinated fibers were 0.55 +/- 0.17 micrometer in FLT(n=5), 0.45 +/- 0.10 micrometer in AGC(n=5), and O.47 +/- 0.06 micrometer meter in VIV(n=5). There was no significant difference among three groups (unpared t-test). The results suggest that there is no effect of space environment on the myelin formation of each nerve fiber in the aortic nerve.     

IEEE 802.11b Ad Hoc Networks: Performance Measurements

In this paper we investigate the performance of IEEE 802.11b ad hoc networks by means of an experimental study. An extensive literature, based on simulation studies, there exists on the performance of IEEE 802.11 ad hoc networks. Our analysis reveals several aspects that are usually neglected in previous simulation studies. Firstly, since different transmission rates are used for control and data frames, different transmission ranges and carrier-sensing ranges may exist at the same time in the network. In addition, the transmission ranges are in practice much shorter than usually assumed in simulation analysis, not constant but highly variable (even in the same session) and depends on several factors. Finally, the results presented in this paper indicate that for correctly understanding the behavior of an 802.11b network operating in ad hoc mode, several different ranges must be considered. In addition to the transmission range, the physical carrier sensing range is very important. The transmission range is highly dependent on the data rate and is up to 100 m, while the physical carrier sensing range is almost independent from the data rate and is approximately 200 m. Furthermore, even though stations are outside from their respective physical carrier sensing range, they may still interfere if their distance is lower than 350 m.Giuseppe Anastasi received the Laurea degree in Electronics Engineering and the Ph.D. degree in Computer Engineering both from the University of Pisa, Italy, in 1990 and 1995, respectively. He is currently an associate professor of Computer Engineering at the Department of Information Engineering of the University of Pisa. His research interests include architectures and protocols for mobile computing, energy management, QoS in mobile networks, and ad hoc networks. He was a co-editor of the book Advanced Lectures in Networking (LNCS 2497, Springer, 2002), and published more than 50 papers, both in international journals and conference proceedings, in the area of computer networking. He served in the TPC of several international conferences including IFIP Networking 2002 and IEEE PerCom 2003. He is a member of the IEEE Computer Society.Eleonora Borgia received the Laurea degree in Computer Engineering from the University of Pisa, Italy, in 2002. She is currently working toward her Ph.D. degree at the IIT Institute of the Italian National Research Council (CNR). Her research interests are in the area of the wireless and mobile networks with particular attention to MAC protocols and routing algorithms for ad hoc networks.Marco Conti received the Laurea degree in Computer Science from the University of Pisa, Italy, in 1987. In 1987 he joined the Italian National Research Council (CNR). He is currently a senior researcher at CNR-IIT. His research interests include Internet architecture and protocols, wireless networks and ad hoc networking, mobile computing, and QoS in packet switching networks. He co-authored the book Metropolitan Area Networks (Springer, London, 1997), and published in journal and conference proceedings more than 100 research papers related to design, modeling, and performance evaluation of computer-network architectures and protocols. He served as the technical program committee chair of the IFIP-TC6 conferences Networking 2002 and PWC 2003, and technical program committee co-chair of ACM WoWMoM 2002. He is serving as technical program committee co-chair of the IEEE Symposium on a World of Wireless, Mobile and Multimedia Networks (WoWMoM 2005). He served as guest editor for the Cluster Computing Journal (special issue on Mobile Ad Hoc Networking), IEEE Transactions on Computers (special issue on Quality of Service issues in Internet Web Services), and ACM/Kluwer Mobile Networks & Applications Journal (special issue on Mobile Ad hoc Networks). He is member of IFIP WGs 6.2, 6.3 and 6.8.Enrico Gregori received the Laurea in electronic engineering from the University of Pisa in 1980. He joined CNUCE, an institute of the Italian National Research Council (CNR) in 1981. He is currently a CNR research director. In 1986 he held a visiting position in the IBM research center in Zurich working on network software engineering and on heterogeneous networking. He has contributed to several national and international projects on computer networking. He has authored more than 100 papers in the area of computer networks and has published in international journals and conference proceedings and is co-author of the book Metropolitan Area Networks (Springer, London, 1997). He was the General Chair of the IFIP TC6 conferences: Networking2002 and PWC2003 (Personal Wireless Communications). He served as guest editor for the Networking2002 journal special issues on: Performance Evaluation, Cluster Computing and ACM/Kluwer Wireless Networks Journals. He is a member of the board of directors of the Create-Net association, an association with several Universities and research centres that is fostering research on networking at European level. He is on the editorial board of the Cluster Computing, of the Computer Networks and of the Wireless Networks Journals. His current research interests include: Wireless access to Internet, Wireless LANs, Quality of service in packet-switching networks, Energy saving protocols, Evolution of TCP/IP protocols.     

Performance evaluation of grouping and regrouping scheme for mitigating hidden station problem in IEEE 802.11ah network

Cluster Computing - An Access Point can support up to 8192 stations with a coverage area of 1 km in the IEEE 802.11ah network. Due to its large communication area, this network severely suffers...     

Access Point Selection Game with Mobile Users Using Correlated Equilibrium

One of the most important issues in wireless local area network (WLAN) systems with multiple access points (APs) is the AP selection problem. Game theory is a mathematical tool used to analyze the interactions in multiplayer systems and has been applied to various problems in wireless networks. Correlated equilibrium (CE) is one of the powerful game theory solution concepts, which is more general than the Nash equilibrium for analyzing the interactions in multiplayer mixed strategy games. A game-theoretic formulation of the AP selection problem with mobile users is presented using a novel scheme based on a regret-based learning procedure. Through convergence analysis, we show that the joint actions based on the proposed algorithm achieve CE. Simulation results illustrate that the proposed algorithm is effective in a realistic WLAN environment with user mobility and achieves maximum system throughput based on the game-theoretic formulation.     

On the oxygen reactivity of flavoprotein oxidases: an oxygen access tunnel and gate in brevibacterium sterolicum cholesterol oxidase

The flavoprotein cholesterol oxidase from Brevibacterium sterolicum (BCO) possesses a narrow channel that links the active center containing the flavin to the outside solvent. This channel has been proposed to serve for the access of dioxygen; it contains at its "bottom" a Glu-Arg pair (Glu-475-Arg-477) that was found by crystallographic studies to exist in two forms named "open" and "closed," which in turn was suggested to constitute a gate functioning in the control of oxygen access. Most mutations of residues that flank the channel have minor effects on the oxygen reactivity. Mutations of Glu-311, however, cause a switch in the basic kinetic mechanism of the reaction of reduced BCO with dioxygen; wild-type BCO and most mutants show a saturation behavior with increasing oxygen concentration, whereas for Glu-311 mutants a linear dependence is found that is assumed to reflect a "simple" second order process. This is taken as support for the assumption that residue Glu-311 finely tunes the Glu-475-Arg-477 pair, forming a gate that functions in modulating the access/reactivity of dioxygen.     

Average Waiting Time of Clusterhead Controlled Token for Virtual Base Station On-Demand in MANETs

A wireless mobile Ad hoc network (MANET) is a collection of wireless mobile hosts forming nodes that are arbitrarily and randomly changing their locations and communicating without the aid of any centralized administration or standard support services. Ad hoc cluster-based routing protocols establish a dynamic wireless mobile infrastructure to mimic the operation of the fixed infrastructure in cellular networks. A clusterhead is elected from a set of nominees, based on an agreed upon rule, to act as a temporary base station within its zone or autonomous system. Mobile stations elected as clusterheads are used to track other mobile stations in the ad hoc network. In each cluster, we use the clusterhead controlled token to assign the channel among contending Mobile Terminals (MTs). A clusterhead controlled token supports multiple class of services and minimizes collisions. In this paper, we derive formulas to calculate the average waiting time for a packet, in order to get transmitted. In our study, we use two polling schemes, namely: Exhaustive polling and Partially Gated polling controlled token.Tarek Sheltami is currently an assistant professor at the Computer Engineering Department at King Fahd University of Petroleum and Minerals (KFUPM) Dhahran, Kingdom of Saudi Arabia. He joined the department on September, 2004. Before joining the KFUPM, Dr. Sheltami was a research associate professor at the School of Information Technology and Engineering (SITE), University of Ottawa, Ontario, Canada. He has two years of industrial experience at GamaEng Inc (2002–2004). He is the co-author of the Warning Energy Aware Clusterhead (WEAC) infrastructure protocol and the Virtual Base Station On-demand (VBS-O) routing protocol. Dr. Sheltami has been a member of a technical program and organizing committees of several international IEEE conferences. Dr. Sheltamis research interests are in the area of wireless communications, wireless ad hoc and sensors networks, mobile infrastructure protocols, network control/mobility management, UMTS, and performance evaluation of wireless communication networks.Hussein Mouftah joined the School of Information Technology and Engineering (SITE) of the University of Ottawa in September 2002 as a Canada Research Chair (Tier 1) Professor in Optical Networks. He has been with the Department of Electrical and Computer Engineering at Queens University (1979–2002), where he was prior to his departure a Full Professor and the Department Associate Head. He has three years of industrial experience mainly at Bell Northern Research of Ottawa, now Nortel Networks (1977–79). He has spent three sabbatical years also at Nortel Networks (1986–87, 1993–94, and 2000–01), always conducting research in the area of broadband packet switching networks, mobile wireless networks and quality of service over the optical Internet. He served as Editor-in-Chief of the IEEE Communications Magazine (1995–97) and IEEE Communications Society Director of Magazines (1998–99) and Chair of the Awards Committee (2002–2003). He is a Distinguished Speaker of the IEEE Communications Society since 2000. Dr. Mouftah is the author or coauthor of five books, 22 book chapters and more than 700 technical papers and 8 patents in this area. He is the recipient of the 1989 Engineering Medal for Research and Development of the Association of Professional Engineers of Ontario (PEO), and the Ontario Distinguished Researcher Award of the Ontario Innovation Trust. He is the joint holder of the Best Paper Award for a paper presented at SPECTS 2002, and the Outstanding Paper Award for papers presented at the IEEE HPSR 2002 and the IEEE ISMVLõ1985. Also he is the joint holder of a Honorable Mention for the Frederick W. Ellersick Price Paper Award for Best Paper in the IEEE Communications Magazine in 1993. He is the recipient of the IEEE Canada (Region 7) Outstanding Service Award (1995). Also he is the recipient of the 2004 IEEE Communications Society Edwin Howard Armstrong Achievement Award, and the 2004 George S. Glinski Award for Excellence in Research of the Faculty of Engineering, University of Ottawa. Dr. Mouftah is a Fellow of the IEEE (1990), the Canadian Academy of Engineering (2003) and the Engineering Institute of Canada (2005).     

Knapsack - TOPSIS Technique for Vertical Handover in Heterogeneous Wireless Network

In a heterogeneous wireless network, handover techniques are designed to facilitate anywhere/anytime service continuity for mobile users. Consistent best-possible access to a network with widely varying network characteristics requires seamless mobility management techniques. Hence, the vertical handover process imposes important technical challenges. Handover decisions are triggered for continuous connectivity of mobile terminals. However, bad network selection and overload conditions in the chosen network can cause fallout in the form of handover failure. In order to maintain the required Quality of Service during the handover process, decision algorithms should incorporate intelligent techniques. In this paper, a new and efficient vertical handover mechanism is implemented using a dynamic programming method from the operation research discipline. This dynamic programming approach, which is integrated with the Technique to Order Preference by Similarity to Ideal Solution (TOPSIS) method, provides the mobile user with the best handover decisions. Moreover, in this proposed handover algorithm a deterministic approach which divides the network into zones is incorporated into the network server in order to derive an optimal solution. The study revealed that this method is found to achieve better performance and QoS support to users and greatly reduce the handover failures when compared to the traditional TOPSIS method. The decision arrived at the zone gateway using this operational research analytical method (known as the dynamic programming knapsack approach together with Technique to Order Preference by Similarity to Ideal Solution) yields remarkably better results in terms of the network performance measures such as throughput and delay.     

Ionworks HT: a new high-throughput electrophysiology measurement platform

To address the throughput restrictions of classical patch clamp electrophysiology, Essen Instruments has developed a plate-based electrophysiology measurement platform. The instrument is an integrated platform that consists of computer-controlled fluid handling, recording electronics, and processing tools capable of voltage clamp whole-cell recordings from thousands of individual cells per day. To establish a recording, the system uses a planar, multiwell substrate (a PatchPlate). The system effectively positions 1 cell into a hole separating 2 fluid compartments in each well of the substrate. Voltage control and current recordings from the cell membrane are made subsequent to gaining access to the cell interior by applying a permeabilizing agent to the intracellular side. Based on the multiwell design of the PatchPlate, voltage clamp recordings of up to 384 individual cells can be made in minutes and are comparable to measurements made using traditional electrophysiology techniques. An integrated pipetting system allows for up to 2 additions of modulation agents. Typical throughput, measurement fidelity, stability, and comparative pharmacology of a recombinant voltage-dependent sodium channel (hNav1.3) and a voltage-gated potassium channel (hKv1.5) exogenously expressed in CHO cells are presented. The IonWorks HT device can be used in biophysical and pharmacological profiling of ion channels in an environment compatible with high-capacity screening.     

A Genetic Algorithm for the Bi-Level Topological Design of Local Area Networks

Local access networks (LAN) are commonly used as communication infrastructures which meet the demand of a set of users in the local environment. Usually these networks consist of several LAN segments connected by bridges. The topological LAN design bi-level problem consists on assigning users to clusters and the union of clusters by bridges in order to obtain a minimum response time network with minimum connection cost. Therefore, the decision of optimally assigning users to clusters will be made by the leader and the follower will make the decision of connecting all the clusters while forming a spanning tree. In this paper, we propose a genetic algorithm for solving the bi-level topological design of a Local Access Network. Our solution method considers the Stackelberg equilibrium to solve the bi-level problem. The Stackelberg-Genetic algorithm procedure deals with the fact that the follower’s problem cannot be optimally solved in a straightforward manner. The computational results obtained from two different sets of instances show that the performance of the developed algorithm is efficient and that it is more suitable for solving the bi-level problem than a previous Nash-Genetic approach.     

Optimal Transmission Radius for Flooding in Large Scale Sensor Networks

One of the principal characteristics of large scale wireless sensor networks is their distributed, multi-hop nature. Due to this characteristic, applications such as query propagation rely regularly on network-wide flooding for information dissemination. If the transmission radius is not set optimally, the flooded packet may be holding the transmission medium for longer periods than are necessary, reducing overall network throughput. We analyze the impact of the transmission radius on the average settling time—the time at which all nodes in the network finish transmitting the flooded packet. Our analytical model takes into account the behavior of the underlying contention-based MAC protocol, as well as edge effects and the size of the network. We show that for large wireless networks there exists an intermediate transmission radius which minimizes the settling time, corresponding to an optimal tradeoff between reception and contention times. We also explain how physical propagation models affect small wireless networks and why there is no intermediate optimal transmission radius observed in these cases. The mathematical analysis is supported and validated through extensive simulations.Marco Zuniga is currently a PhD student in the Department of Electrical Engineering at the University of Southern California. He received his Bachelors degree in Electrical Engineering from the Pontificia Universidad Catolica del Peru in 1998, and his Masters degree in Electrical Engineering from the University of Southern California in 2002. His interests are in the area of Wireless Sensor Networks in general, and more specifically in studying the interaction amongst different layers to improve the performance of these networks. He is a member of IEEE and the Phi Kappa Phi Honor society.Bhaskar Krishnamachari is an Assistant Professor in the Department of Electrical Engineering at the University of Southern California (USC), where he also holds a joint appointment in the Department of Computer Science. He received his Bachelors degree in Electrical Engineering with a four-year full-tuition scholarship from The Cooper Union for the Advancement of Science and Art in 1998. He received his Masters degree and his Ph.D. in Electrical Engineering from Cornell University in 1999 and 2002, under a four-year university graduate fellowship. Dr. Krishnamacharis previous research has included work on critical density thresholds in wireless networks, data centric routing in sensor networks, mobility management in cellular telephone systems, multicast flow control, heuristic global optimization, and constraint satisfaction. His current research is focused on the discovery of fundamental principles and the analysis and design of protocols for next generation wireless sensor networks. He is a member of IEEE, ACM and the Tau Beta Pi and Eta Kappa Nu Engineering Honor Societies     

Simple approaches to close the open structure of microfluidic chips and connecting them to the macro-world

Microchip electrophoresis has a great potential to improve the speed and throughput of chemical and biochemical analyses. Conventional electrophoresis microchip fabrication methods comprise the main steps of channel formation, cover plate binding and access hole construction. While the fabrication of appropriate cover plates and their bonding process are quite essential to the creation of closed microfluidic networks, connection means of microchips to the macro-world is one of the most important parts of microchip fabrication. In this paper the most commonly used approaches are discussed for cover plate connector fabrication in conjunction with high and low temperature glue-less binding processes. The microchannels in the glass substrate were fabricated by sawing and powder blasting under regular laboratory settings, i.e., not necessitating the use of a clean-room environment, making in this way broader availability for electrophoresis microchip technology.     

Proxy based seamless connection management method in mobile cloud computing

Nowadays, complex smartphone applications are developed that support gaming, navigation, video editing, augmented reality, and speech recognition which require considerable computational power and battery lifetime. The cloud computing provides a brand new opportunity for the development of mobile applications. Mobile Hosts (MHs) are provided with data storage and processing services on a cloud computing platform rather than on the MHs. To provide seamless connection and reliable cloud service, we are focused on communication. When the connection to cloud server is increased explosively, each MH connection quality has to be declined. It causes several problems: network delay, retransmission, and so on. In this paper, we propose proxy based architecture to improve link performance for each MH in mobile cloud computing. By proposed proxy, the MH need not keep connection of the cloud server because it just connected one of proxy in the same subnet. And we propose the optimal access network discovery algorithm to optimize bandwidth usage. When the MH changes its point of attachment, proposed discovery algorithm helps to connect the optimal access network for cloud service. By experiment result and analysis, the proposed connection management method has better performance than the 802.11 access method.     

Metabolic Implications of Exposure to Light at Night: Lessons from Animal and Human Studies

Lately, the incidence of overweight, obesity, and type 2 diabetes has shown a staggering increase. To prevent and treat these conditions, one must look at their etiology. As life on earth has evolved under the conditions of nature’s 24‐hour light/dark cycle, it seems likely that exposure to artificial light at night (LAN) would affect physiology. Indeed, ample evidence has shown that LAN impacts many metabolic parameters, at least partly via the biological clock in the suprachiasmatic nucleus of the hypothalamus. This review focuses on the impact of chronic and acute effects of LAN of different wavelengths on locomotor activity, food intake, the sleep/wake cycle, body temperature, melatonin, glucocorticoids, and glucose and lipid metabolism. While chronic LAN disturbs daily rhythms in these parameters, experiments using short‐term LAN exposure also have shown acute negative effects in metabolically active peripheral tissues. Experiments using LAN of different wavelengths not only have indicated an important role for melanopsin, the photopigment found in intrinsically photosensitive retinal ganglion cells, but also provided evidence that each wavelength may have a specific impact on energy metabolism. Importantly, exposure to LAN has been shown to impact glucose homeostasis also in humans and to be associated with an increased incidence of overweight, obesity, and atherosclerosis.     

Cell to aperture interaction in patch-clamp chips visualized by fluorescence microscopy and focused-ion beam sections

Patch-clamp is an important method to monitor the electrophysiological activity of cells and the role of pharmacological compounds on specific ion channel proteins. In recent years, planar patch-clamp chips have been developed as a higher throughput approach to the established glass-pipette method. However, proper conditions to optimize the high resistance cell-to-probe seals required to measure the small currents resulting from ion channel activity are still the subject of conjecture. Here, we report on the design of multiple-aperture (sieve) chips to rapidly facilitate assessment of cell-to-aperture interactions in statistically significant numbers. We propose a method to pre-screen the quality of seals based on a dye loading protocol through apertures in the chip and subsequent evaluation with fluorescence confocal microscopy. We also show the first scanning electron micrograph of a focused ion beam section of a cell in a patch-clamp chip aperture.