共查询到20条相似文献,搜索用时 31 毫秒
1.
2.
3.
4.
Expression of the essential mRNA export factor Yra1p is autoregulated by a splicing-dependent mechanism 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《RNA (New York, N.Y.)》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Recent evidence supports the idea that pre-mRNA splicing and mRNA export are mechanistically coupled. In metazoans, this process appears to be mediated by a multicomponent complex, which associates with the spliced RNA upstream of the exon-exon junction. One of these components (Aly/REF) has a homolog in the budding yeast Saccharomyces cerevisiae known as Yra1p. The YRA1 gene is essential for growth and required for mRNA export. Notably, YRA1 is one of the only approximately 5% intron-containing genes in yeast. Moreover, the YRA1 intron has several unusual features and is conserved in other budding yeast species. Previously, overexpression of intronless YRA1 was shown to be toxic. We show here that overexpression of the intron-containing gene results in increased levels of unspliced pre-mRNA but normal levels of Yra1 protein; conversely, expression of the cDNA results in increased levels of protein and accumulation of nuclear poly(A)+ RNA. Two additional lines of evidence suggest that expression of Yra1p is autoregulated: First, expression of excess Yra1p from a plasmid reduces the level of tagged, chromosomal Yra1p, and, second, this effect requires wild-type protein. Replacement of the YRA1 intron with that of other S. cerevisiae genes cannot rescue the dominant-negative growth defect of intronless YRA1. We conclude that the level of Yra1p is negatively autoregulated by a mechanism that involves splicing of its unusual intron. Tight control of the levels of Yra1p might be necessary to couple the rates of pre-mRNA splicing and mRNA export. 相似文献
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
An intron in the YRA1 gene is required to control Yra1 protein expression and mRNA export in yeast 总被引:2,自引:0,他引:2
下载免费PDF全文
![点击此处可从《EMBO reports》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Yra1p is an essential and conserved mRNA export factor in yeast. Strikingly, removal of the intron from YRA1 causes a dominant-negative growth phenotype and a concomitant inhibition of mRNA export. However, both defects are neutralized by replacement of the intron of YRA1 by a different intron. Significantly, Yra1p is overproduced in yeast when expressed from its intronless gene, but Yra1p levels are the same as the wild type when expressed from an intron-containing YRA1 gene. Thus, an intron in YRA1 controls Yra1p expression and mRNA export. 相似文献
18.
19.