共查询到20条相似文献,搜索用时 15 毫秒
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Bernhard Horsthemke Karl Bauer 《Biochemical and biophysical research communications》1981,103(4):1322-1328
An enzyme that hydrolyzes the fluorogenic chymotrypsin substrate glutaryl-Gly-Gly-Phe-β-naphthylamide has been partially purified from extracts of bovine anterior pituitaries. Like chymotrypsin, this enzyme hydrolyzes the neuropeptide Luliberin (LH-RF, <Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2) at the carboxyl-side of Trp and Tyr, but it differs from the pancreatic protease by its high molecular weight, insensitivity towards OH-reactive agents and other enzymechemical parameters. It seems, however, to be identical to the “cation-sensitive neutral endopeptidase”. In the course of this study evidence has also been obtained that LH-RF is not degraded by the cystinyl-arylamidase. 相似文献
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Mary E. Bayliss Stella H. Wilkes John M. Prescott 《Archives of biochemistry and biophysics》1980,204(1):214-219
Kinetic measurements on the action of Aeromonas neutral protease toward blocked peptide substrates were made in order to determine the most favorable fit on the enzyme subsites that bind the residues flanking the scissile bond and to define the number of secondary sites involved in catalysis. Variations in the identity of P1′,3 the residue furnishing the amino group to the scissile bond, produced significant changes in krmcat, whereas the identity of P1′, the residue donating the carboxyl group, was of much less catalytic importance. Comparison of these results with those of previous investigators of other bacterial neutral proteases indicated distinct differences in specificity of the Aeromonas enzyme and revealed that phenylalanyl residues, rather than leucyl, were preferred in the P1′ position. Additional binding sites on the carboxyl side of the scissile bond were shown to be important to catalytic efficiency and it is evident that at least three residues (P1t P2′, P3′) are involved while only two residues (P2, P1) on the amino terminal side of the sensitive bond are implicated. 相似文献
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M.D. Guasch M. Plana E. Itarte 《Biochemical and biophysical research communications》1982,107(1):82-88
The heat-labile inhibitor of casein kinases 1 and 2 from rat liver cytosol (J.F. Bertomeu , FEBS Lett., , 262–264) has been purified extensively and characterized. Analysis by gel filtration and SDS-polyacrylamide gel electrophoresis suggest that the inhibitor has an Mr of 30,000. It did not contain glycosaminoglycans, oligonucleotides or neutral sugars and was totally inactivated by digestion with trypsin. Besides casein kinases, the inhibitor also inhibited the catalytic subunit of cAMP-dependent protein kinase to the same extent. The data suggest that the inhibitor is a monomeric protein that could modulate intracellular protein phosphorylation by both casein kinases and cAMP-dependent protein kinase. 相似文献
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Norman A. Nelson R.W. Jackson A.T. Au D.J. Wynalda E.E. Nishizawa 《Prostaglandins & other lipid mediators》1975,10(5):795-806
The synthesis of -4,5,6-trinor-3,7-inter--phenylene-3-oxaprostaglandins oxaprostaglandins of the E1 and F1α series7 from 6-endo-(1-heptenyl)-bicyclo[3:1:0]hexan-3-one (III), is described. Preliminary biological screening data for gerbil colon smooth muscle stimulation, rat blood pressure and substrate specificity toward 15-hydroxyprostaglandin dehydrogenase is presented. Platelet function studies, both and of -4,5,6-trinor-3,7-inter--phenylene-3-oxa-PGE1, methyl ester (VIII) are presented. 相似文献