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1.
A gas chromatographic method for the determination of aldose and uronic Acid constituents of plant cell wall polysaccharides 总被引:25,自引:14,他引:11
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A major problem in determining the composition of plant cell wall polysaccharides has been the lack of a suitable method for accurately determining the amounts of galacturonic and glucuronic acids in such polymers. A gas chromatographic method for aldose analysis has been extended to include uronic acids. Cell wall polysaccharides are depolymerized by acid hydrolysis followed by treatment with a mixture of fungal polysaccharide-degrading enzymes. The aldoses and uronic acids released by this treatment are then reduced with NaBH4 to alditols and aldonic acids, respectively. The aldonic acids are separated from the alditols with Dowex-1 (acetate form) ion exchange resin, which binds the aldonic acids. The alditols, which do not bind, are washed from the resin and then acetylated with acetic anhydride to form the alditol acetate derivatives. The aldonic acids are eluted from the resin with HCl. After the resin has been removed, the HCl solution of the aldonic acids is evaporated to dryness, converting the aldonic acids to aldonolactones. The aldonolactones are reduced with NaBH4 to the corresponding alditols, dried and acetylated. The resulting alditol acetate mixtures produced from the aldoses and those from the uronic acids are analyzed separately by gas chromatography. This technique has been used to determine the changes in composition of Red Kidney bean (Phaseolus vulgaris) hypocotyl cell walls during growth, and to compare the cell wall polysaccharide compositions of several parts of bean plants. Galacturonic acid is found to be a major component of all the cell wall polysaccharides examined. 相似文献
2.
Sugar constituents of fucose-containing polysaccharides from various Japanese brown algae 总被引:3,自引:0,他引:3
Sugar constituents of the fucose-containing polysaccharides (FCPs) from 21 species of brown algae were analyzed. FCPs were extracted with hot water (100 °C, 4 h), separated by precipitation with 20% (v:v) ethanol in the presence of 0.05 M MgCl2 to remove contaminating soluble alginate, and purified by DEAE-Sephadex column chromatography. The samples were hydrolyzed with HCI, and neutral sugar and uronic acid were separated by anion exchange chromatography. Their amounts were determined by gas-liquid chromatography. The neutral sugars in the FCPs from Ishige okamurae, Laminaria ochotensis, Myelophycus simplex, Padina arborescens and Sargassum thunbergii all contained arabinose, fucose, galactose, glucose, mannose, rhamnose and xylose residues. The FCPs from Ishige okamurae, Padina arborescens, Sargassum hemiphyllum, S. patents and S. sagamianum contained the four uronic acids, galacturonic acid, glucuronic acid, guluronic acid and mannuronic acid. 相似文献
3.
This report describes a procedure for analyzing glucuronic and iduronic acids using the Technicon automated sugar chromatography system. Glueronic and iduronic acids of standard samples of glycosaminoglycans have been analyzed after hydrolysis by formic acid. The method has been applied to quantitate uronic acids in chondroitin sulfates and dermatan sulfate mixtures obtained by Dowex 1 Cl? column fractionation of glycosaminoglycans from aortas of different animal species. The results are in good agreement with those obtained by the gas-liquid chromatographic technique. 相似文献
4.
Anthony B. Blakeney Philip J. Harris Robert J. Henry Bruce A. Stone 《Carbohydrate research》1983,113(2):291-299
A simple and rapid method is described for the preparation of alditol acetates from monosaccharides. It can be performed in a single tube without transfers or evaporations. Monosaccharides are reduced with sodium borohydride in dimethyl sulphoxide and the resulting alditols acetylated using 1-methylimidazole as the catalyst. Removal of borate is unnecessary and acetylation is complete in 10 min at room temperature. Monosaccharides are quantitatively reduced and acetylated by this procedure. The alditol acetates are completely separated by glass-capillary, gas-liquid chromatography on Silar 10C. The method has been applied to the analysis of monosaccharides in acid hydrolysates of a plant cell-wall. 相似文献
5.
Estimations of Uronic Acids as Quantitative Measures of Extracellular and Cell Wall Polysaccharide Polymers from Environmental Samples 总被引:4,自引:3,他引:1
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Steven A. Fazio David J. Uhlinger Jeffrey H. Parker David C. White 《Applied microbiology》1982,43(5):1151-1159
The extracellular polysaccharide polymers can bind microbes to surfaces and can cause physical modification of the microenvironment. Since uronic acids appear to be the components of these extracellular films that are most concentrated in a location outside the cell membrane, a quantitative assay for uronic acids was developed. Polymers containing uronic acids are resistant to quantitative hydrolysis, and the uronic acids, once released, form lactones irreproducibly and are difficult to separate from the neutral sugars. These problems were obviated by the methylation of the uronic acids and their subsequent reduction with sodium borodeuteride to the corresponding alcohol while they were in the polymer and could not form lactones. This caused the polymers to lose the ability to adhere to their substrates, so they could be quantitatively recovered. The hydrolysis of the dideuterated sugars was reproducible and could be performed under conditions that were mild enough that other cellular and extracellular polymers were not affected. The resulting neutral sugars were readily derivatized and then were separated and assayed by glass capillary gas-liquid chromatography. The dideuterated portion of each pentose, hexose, or heptose, identified by combined capillary gas-liquid chromatography and mass spectrometry, accurately provided the proportion of each uronic acid in each carbohydrate of the polymer. Examples of the applications of this methodology include the composition of extracellular polymers in marine bacteria, invertebrate feeding tubes and fecal structures, and the microfouling films formed on titanium and aluminum surfaces exposed to seawater. 相似文献
6.
Mulberry tree leaves were shown to have mucilaginous polysaccharides. The extracted water-soluble mucilage was separated into three fractions via a cetylpyridinum chloride complex and purified by anion-exchange chromatography. Five acidic polysaccharides were separated from these fractions, one of which was a major polysaccharide (Mp-3) that was structurally analyzed and used for antibody preparation. The Mp-3 polysaccharide contained rhamnose, galactose, glucose, galacturonic acid, and glucuronic acid in a molar ratio of 1 : 0.2 : 0.5 : 2.3 : 1.5 as constituent monosaccharides. Methylation and gas chromatography-mass spectrometry analysis indicated that the polysaccharide was a rhamnogalacturonan mainly consisting of 1,2,3-linked rhamnose residues, 1,3,4- and 1,4-linked uronic acid residues, and terminal uronic acid residues. Its molecular weight was estimated to be 5.5 x 10(5). Immunohistological observation revealed that the Mp-3 polysaccharide is specifically localized in inner epidermal cells situated in adaxial leaves, and electron microscopy showed that its subcellular location is between the plasma membrane and the cell wall. In young leaves, numerous secretory vesicles were present in a shrunken cytoplasm that was surrounded by fibers. In mature leaves, more than 20% of total epidermal cells were these inner cells in which polysaccharide deposition was significantly increased. The deposits appeared as a rounded electron-dense mass throughout the inner cells by electron microscopy. 相似文献
7.
The factors affecting the stability, hydrolysis, reduction, acetylation, quantitation, and identification of the neutral sugars from vegetable fiber preparations have been studied critically and optimized. The recommended method offers a consolidation of the recent modifications of the alditol acetate procedure for the estimation of neutral sugars. The recovery of the sugars was tested by glc and ion-exchange chromatography. Also, the modified carbazole method of Bitter and Muir was adapted to make it applicable for the estimation of uronic acid content of fiber because uronic acid cannot be estimated quantitatively by the acetylation procedure. It is emphasized that the proposed method is applicable only to highly purified fiber preparations which are free of coprecipitated intracellular compounds. Also, the levels of pentoses and hexoses in the fiber must be well defined and a suitable correction made for their interference in the assay. 相似文献
8.
A novel,highly viscous polysaccharide excreted by an alteromonas isolated from a deep-sea hydrothermal vent shrimp 总被引:4,自引:0,他引:4
Raguénès G Cambon-Bonavita MA Lohier JF Boisset C Guezennec J 《Current microbiology》2003,46(6):448-452
A deep-sea, mesophilic, aerobic, and heterotrophic microorganism, able to produce an extracellular polysaccharide, was isolated from a shrimp collected near an active hydrothermal vent of the Mid-Atlantic Ridge. On the basis of phenotypic and phylogenetic analyses and DNA/DNA relatedness, this strain could be assigned to the species Alteromonas macleodii as a variant of the fijiensis subspecies. It was selected for its ability to exhibit a swarming mucoid phenotype on specific media. The bacterium secreted, under laboratory conditions, an extremely viscous exopolysaccharide consisting of glucose, galactose as neutral sugars, and glucuronic, galacturonic acids as uronic acids, along with pyruvate and acetate as main substituents. 相似文献
9.
A new core tetrasaccharide component from the lipopolysaccharide of Rhizobium trifolii ANU 843 总被引:5,自引:0,他引:5
R I Hollingsworth R W Carlson F Garcia D A Gage 《The Journal of biological chemistry》1989,264(16):9294-9299
A second core oligosaccharide fragment has been isolated and characterized from the lipopolysaccharide (LPS) of Rhizobium trifolii ANU 843. The oligosaccharide is a tetrasaccharide composed of galactose, galacturonic acid, mannose, and 3-deoxy-D-manno-2-octulosonic acid. The mannose residue is alpha-linked to the 4-position of 3-deoxy-D-manno-2-octulosonic acid and the galacturonic acid residue is alpha-linked to the 6-position of mannose. The galactose residue, which is acetylated at the 4-position, is attached to the 4-position of mannose by an alpha-linkage. All of the aldoses are in the pyranose form. The composition of the tetrasaccharide was determined by gas-liquid chromatography of the alditol acetate derivatives of the component monosaccharides. The configuration of anomeric linkages was determined by 1H NMR spectroscopy. Fast atom bombardment-mass spectrometry (FAB-MS) was performed on acetylated, per(trideutero)acetylated and underivatized tetrasaccharide giving sequence information in addition to information on the residue which was acetylated. Similar studies were performed on the oligosaccharide after reduction with sodium cyanoborohydride and peracetylation with labeled and unlabeled acetic anhydride as before. Further linkage and sequence analysis was obtained from methylation analysis, and from electron impact mass spectrometry of the per(trideutero)acetylated oligosaccharide and from collision-induced dissociation fast atom bombardment tandem mass spectrometry using linked scans at constant B/E on the cyanoborohydride-reduced, per (trideutero)acetylated oligosaccharide. The exact location of the acetyl group was deduced from 1H NMR double resonance experiments in conjunction with mass spectrometric data. 相似文献
10.
A new electrophoretic method using Titan III cellulose acetate plates has been developed for the separation and quantitation of glucuronic acid and iduronic acid. This method is quite simple, and glucuronic acid and iduronic acid can be separated within 50 min. This method was applied to the analyses of uronic acids in chondroitin sulfates A and C, and dermatan sulfate. 相似文献
11.
Widmer W 《Biotechnology letters》2011,33(2):365-368
While the most accurate method for analysis of sugars in biomass is based on gas chromatography of trimethylsilane or alditol
acetate derivatives of sugars, the derivation method is time consuming and laborious. In comparison, sample preparation for
sugar analysis of hydrolyzed biomass samples using liquid chromatography is a simple dilution procedure with water. A gradient
HPLC method using a anion-exchange column and pulsed-amperometric detection modified to reduce analysis time from 75 to 40 min
was further improved. The new method no longer requires post-column addition to stablilize the baseline using a pulsed-amperometric
detector with the mobile phase gradient. The method provides good resolution of arabinose, rhamnose, galactose, xylose, glucose,
fructose, sucrose, cellobiose, and galacturonic acid in both standards and hydrolyzed citrus waste materials. By changing
the waveform used with the PAD detector, the requirement for post-column addition was eliminated while maintaining a stable
baseline. 相似文献
12.
The use of a single, commercially available column packing, TabsorbR, is described for the g.l.c. separation of a large number of different compounds. The resolution of the homologous members of the following series of compounds was achieved: (1) saturated fatty acids (C1-C18), (2) normal aliphatic saturated dicarboxylic acids (C2-C14), (3) normal aliphatic saturated alcohols (C1-C24), (4) normal aliphatic saturated amines (C1-C12), (5) the common amino acids except arginine, histidine and cysteine, (6) aliphatic hydrocarbons (C10-C20) and (7) monosaccharides. It should be noted that twenty-two monosaccharides including three hexosamines and two anhydrohexoses, could be resolved as alditol acetates in a single run. In addition, galacturonic, glucuronic and iduronic acids could be separated from one another as their 1,4-lactones. The resolution achieved in these series of compounds was found to be consistent and highly reproducible. It is of further interest that certain isomers of the higher fatty acids and hydrocarbons with one double bond could also be separated from the normal and saturated compounds, respectively. The applicability of "Tabsorb" for the g.l.c. separation, although noted above to be considerably broad, is by far not yet exhausted. These procedures which form the basis for the quantitative determinations of the various compounds studied as demonstrated by analysis of glycopeptides for neutral hexoses and proteins for the amino acids, can readily be adapted to preparative methods. From the biochemical point of view "Tabsorb" is an extremely versatile column packing in that it can be used for the identification of many of the common building blocks of natural products. 相似文献
13.
Gérard Raguénès Marie Anne Cambon-Bonavita Jean François Lohier Claire Boisset Jean Guezennec 《Current microbiology》2003,46(6):0448-0452
A deep-sea, mesophilic, aerobic, and heterotrophic microorganism, able to produce an extracellular polysaccharide, was isolated
from a shrimp collected near an active hydrothermal vent of the Mid-Atlantic Ridge. On the basis of phenotypic and phylogenetic
analyses and DNA/DNA relatedness, this strain could be assigned to the species Alteromonas macleodii as a variant of the fijiensis subspecies. It was selected for its ability to exhibit a swarming mucoid phenotype on specific media. The bacterium secreted,
under laboratory conditions, an extremely viscous exopolysaccharide consisting of glucose, galactose as neutral sugars, and
glucuronic, galacturonic acids as uronic acids, along with pyruvate and acetate as main substituents.
Received: 10 July 2002 / Accepted: 13 August 2002 相似文献
14.
Usov AI Smirnova GP Kamenarska Z Dimitrova-Konaklieva S Stefanov KL Popov SS 《Bioorganicheskaia khimiia》2004,30(2):182-189
GC-MS of trimethylsilyl derivatives of the compounds present in the butanolic extract of biomass of brown seaweed Colpomenia peregrina from the Black Sea aided in identification of 24 components, including aliphatic hydroxy and keto and aromatic acids, glycerol, mannitol, floridoside, and monosaccharides. The polysaccharide composition of the biomass was also studied, with high sodium alginate and laminaran contents and a comparatively low level of fucoidan being revealed. The polysaccharides were isolated from the biomass by fractional extraction and purified by precipitation or ion exchange chromatography. The structures of alginic acid and laminaran were deduced from 13C NMR spectra and confirmed, in the case of laminaran, by methylation analysis. The sodium alginate was shown to contain more guluronic (G) than mannuronic acid (M) residues, the M/G ratio being 0.48. Laminaran was demonstrated to be a beta-glucan with 1-->3 linkages in its backbone and 1-->6 linkages in its branching points, which is characteristic of brown algae. Fucoidan turned out to be a complex heteropolysaccharide containing, in addition to fucose and sulfate, other neutral monosaccharides and uronic acids. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 2; see also http://www.maik.ru 相似文献
15.
Lamia Trabelsi Nour Houda M’sakni Hatem Ben Ouada Hassen Bacha Sadok Roudesli 《Biotechnology and Bioprocess Engineering》2009,14(1):27-31
In this study, the physico-chemical characteristics of extracellular polysaccharides (EPS) produced by Arthrospira platensis were evaluated. Elemental analysis and a bicinchoninic acid (BCA) reaction indicated that the EPS were heteropolysaccharides
that contain carbohydrate (13%) and protein (55%) moieties. Analysis of the infrared spectrum and elemental analysis revealed
the presence of a sulfate group (0.5%). The UV-visible spectrum showed high UV absorption at 190∼230 nm and a shoulder at
260∼280 nm. In addition, this spectrum indicated that EPS can form aggregates with mycosporine-like amino acids and/or scytonemin.
Gas chromatography analysis of the carbohydrate portion of the EPS indicated that it was composed of seven neutral sugars:
galactose (14.9%), xylose (14.3%), glucose (13.2%), frucose (13.2%), rhamnose (3.7%), arabinose (1%), and mannose (0.3%) and
two uronic acids, galacturonic acid (13.5%) and glucuronic acid (0.9%). 相似文献
16.
Ultrastructure and chemical composition of the sheath of Leptothrix discophora SP-6. 总被引:2,自引:1,他引:1
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Light microscopy and transmission electron microscopy of thin sections and metal-shadowed specimens showed that the sheath of Leptothrix discophora SP-6 (ATCC 51168) is a tube-like extracellular polymeric structure consisting of a condensed fabric of 6.5-nm-diameter fibrils underlying a more diffuse outer capsular layer. In thin sections, outer membrane bridges seen to contact the inner sheath layer suggested that the sheath fabric was attached to the outer layer of the gram-negative cell wall. The capsular polymers showed an affinity for cationic colloidal iron and polycationic ferritin, indicating that they carry a negative charge. Cell-free sheaths were isolated by treatment with a mixture of lysozyme, EDTA, and N-lauroylsarcosine (Sarkosyl) or sodium dodecyl sulfate (SDS). Both Sarkosyl- and SDS-isolated sheaths were indistinguishable in microscopic appearance. However, the Mn-oxidizing activity of Sarkosyl-isolated sheaths was more stable than that of SDS-isolated sheaths. The Sarkosyl-isolated sheaths also contained more 2-keto-3-deoxyoctanoic acid and more outer membrane protein than SDS-isolated sheaths. The oven-dried mass of detergent-isolated sheaths represented approximately 9% of the total oven-dried biomass of SP-6 cultures; the oven-dried sheaths contained 38% C, 6.9% N, 6% H, and 2.1% S and approximately 34 to 35% carbohydrate (polysaccharide), 23 to 25% protein, 8% lipid, and 4% inorganic ash. Gas-liquid chromatography showed that the polysaccharide was an approximately 1:1 mixture of uronic acids (glucuronic, galacturonic, and mannuronic acids and at least one other unidentified uronic acid) and an amino sugar (galactosamine). Neutral sugars were not detected. Amino acid analysis showed that sheath proteins were enriched in cysteine (6 mol%). The cysteine residues in the sheath proteins probably provide sulfhydryls for disulfide bonds that play an important role in maintaining the structural integrity of the sheath (D. Emerson and W.C. Ghiorse, J. Bacteriol. 175:7819-7827, 1993). 相似文献
17.
A proteoglycan was isolated from the red alga Laurencia spectabilis Postels & Ruprecht (Ceramiales) by extraction in a dilute buffer—NaCl solution followed by gel and anion exchange chromatography. The molecule was composed of 92% carbohydrate and 8% protein. Galactose and uronic acids were the major monosaccharides. Ester sulfate was not detected. A small quantity of hydroxyproline was present in the protein component. The proteoglycan accounted for a very small portion (less than 1% by fr. wt) of the alga. 相似文献
18.
Characterization of the carbohydrate component of fraction I in the Neurospora crassa cell wall.
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The carbohydrate portion of fraction I of the Neurospora crassa cell wall has been analyzed for sugar composition by gas-liquid chromatography and colorimetric methods. The analysis was performed comparatively in a wild-type strain (RL 3-8A) and three morphological mutants: scumbo (FGSC 49), peak-2a (a mutant known to be allelic to biscuit), and ragged (FGSC 296). Fraction I of all strains studied contains glucose, mannose, and galactose as the main sugars. Uronic acids and amino sugars are also present in small amounts. The glycosidic linkages binding the neutral sugars were analyzed by Lindberg's combined gas chromatography-mass spectrometry techniques for identification of the partially methylated alditol acitate sugar derivatives. The main polymeric portion of fraction I seems to be a linear glucan with the glucose residues linked by 1 leads to 3 and 1 leads to 4 bonds. A mannan portion with a branched configuration is also present, with galactose as the sugar residue which serves as branches in the molecule(s). The branched mannan portion appears to increase in amount in correlation with more drastic morphological changes of the mycelia. In this respect, the mutant ragged has the lowest mycelial growth rate and the largest amount of mannan. The importance of the polysaccharide structure of fraction I on the colonial morphology of the mycelia is discussed. 相似文献
19.
A rapid and sensitive method for the analysis of carbohydrate components in glycoproteins using gas-liquid chromatography 总被引:19,自引:0,他引:19
M F Chaplin 《Analytical biochemistry》1982,123(2):336-341
A rapid and simple gas-liquid chromatographic method for the determination of subnanomolar amounts of carbohydrates derived from glycoproteins is described. The procedure involves methanolysis in the presence of methyl acetate followed by removal of hydrogen chloride by coevaporation with t-butyl alcohol and trimethylsilylation. The method is also applicable to samples containing uronic acids and lipids. 相似文献
20.
Analysis of bacterial exopolysaccharides 总被引:4,自引:0,他引:4
Extracellular polysaccharides have been isolated from cultures of freshwater and marine bacteria originally isolated from material adhering to surfaces and underivatized hydrolysates have been analyzed by high-performance liquid chromatography methods. A scheme has been developed whereby the uronic acids can be identified on strong anion-exchange columns, while neutral monosaccharides can be separated and identified using aminobonded columns or cation-exchange adsorbent loaded with a heavy metal ion. The methods permit rapid and accurate comparison of polysaccharides with differing chemotype. The strains studied show a range of different chemotypes, all containing a uronic acid and several neutral monosaccharides. Some of the polysaccharides isolated from marine bacteria possessed a very high acetyl content. 相似文献