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1.
A novel actinomycete, strain NEAU-st1T, was isolated from a soil sample collected in Shaanxi province, Northwest China and characterized using a polyphasic approach. 16S rRNA gene sequence similarity studies showed that strain NEAU-st1T belongs to the genus Nonomuraea, being most closely related to Nonomuraea rosea GW12687T (98.91 %), Nonomuraea solani NEAU-Z6T (98.44 %), Nonomuraea rhizophila YIM67092T(98.24 %) and Nonomuraea monospora PT708T (98.02 %); similarities to sequences of other type strains of the genus Nonomuraea were lower than 98 %. Both tree-making algorithms used also supported the position that strain NEAU-st1T formed a distinct clade with its most closely related species. Morphological and physiological characteristics confirmed that the strain belongs to the genus Nonomuraea and distinguished it from its most closely related species. DNA–DNA hybridization further differentiated strain NEAU-st1T from its nearest phylogenetic neighbours. These results suggested that strain NEAU-st1T represents a novel species of the genus Nonomuraea, for which the name Nonomuraea shaanxiensis sp. nov. is proposed. The type strain is NEAU-st1T (=CGMCC 4.7096T = DSM 45877T).  相似文献   

2.
The taxonomic status of a moderately halophilic bacterium, strain N4T, isolated from soil of a chicken farm in China was determined. It was Gram-negative, non-spore-forming, motile, and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequence indicated that this strain belonged to the genus Salinicola, as it showed the highest sequence similarities to Salinicola salaries M27T (98.3 %), Salinicola socius SMB35T (98.1 %), and Salinicola halophilus CG4.1T (98.1 %). The major cellular fatty acids were C16:0 (25.6 %), C18:1ω7c (35.0 %), and C19:0 cyclo ω8c (11.9 %), which are properties shared by members of the genus Salinicola. The DNA G+C content of strain N4T was 69.1 mol %. The level of DNA–DNA relatedness between strain N4T and the other three type strains of the genus of Salinicola salaries M27T, Salinicola socius SMB35T, and Salinicola halophilus CG4.1T were 34.3, 28.7, and 26.9 %, respectively. Based on the results of phenotypic, chemotaxonomic, DNA–DNA relatedness, and phylogenetic analysis, strain N4T should be classified as a novel species of the genus Salinicola, for which the name Salinicola zeshunii sp. nov. is proposed, with strain N4T (=KACC 16602T = CCTCC AB 2012912T) as the type strain.  相似文献   

3.
A novel Gram-positive, rod-shaped, motile, spore-forming, nitrogen-fixing bacterium, designated strain 112T, was isolated from cabbage rhizosphere in Beijing, China. The strain was found to grow at 10–40 °C and pH 4–11, with an optimum of 30 °C and pH 7.0, respectively. Phylogenetic analysis based on a fragment of the full-length 16S rRNA gene sequence revealed that strain 112T is a member of the genus Paenibacillus. High levels of 16S rRNA gene similarities were found between strain 112T, Paenibacillus sabinae DSM 17841T (97.82 %) and Paenibacillus forsythiae DSM 17842T (97.22 %). However, the DNA–DNA hybridization values between strain 112T and the type strains of these two species were 10.36 and 6.28 %, respectively. The predominant menaquinone was found to be menaquinone 7 (MK-7). The major fatty acids were determined to be anteiso-C15:0 and C16:0. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and unknown aminophospholipids. The cell wall peptidoglycan was found to contain meso-diaminopimelic acid. The DNA G+C content was determined to be 55.4 mol%. On the basis of its phenotypic characteristics, 16S rRNA gene sequence analysis and the value of DNA–DNA hybridization, strain 112T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus brassicae sp. nov. is proposed. The type strain is 112T (= ACCC 01125T = DSM 24983T).  相似文献   

4.
An actinomycete capable of lysing cyanobacteria, strain JXJ 0074T, was isolated from a soil sample collected from Jiangxi province, south China, and characterized by using polyphasic taxonomy. The new isolate showed morphological and chemotaxonomic properties typical of members of the genus Streptomyces. Phylogenetic analysis of the near-complete 16S rRNA gene sequence indicated that strain JXJ 0074T should be affiliated to the genus Streptomyces and exhibited highest similarities to Streptomyces shenzhenensis DSM 42034T (98.99 %) and Streptomyces lucensis NBRC 13056T (98.60 %), while the similarities to other members of the genus are lower than 98.22 % similarity. However, the DNA–DNA hybridization values between strain JXJ 0074T and S. shenzhenensis DSM 42034T or S. lucensis NBRC 13056T were 46.2 ± 2.6 and 32.6 ± 3.1 %, respectively. Thus, on the basis of the polyphasic data, strain JXJ 0074T represents a novel species of the genus Streptomyces, for which the name Streptomyces jiujiangensis sp. nov. is proposed. The type strain is JXJ 0074T (= BCRC 16953T = KCTC 29262T).  相似文献   

5.
A novel, red-pigmented and coccoid haloarchaeon, designated strain CBA1101T, was isolated from a marine sediment. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CBA1101T is most closely related to the genus Halococcus in the family Halobacteriaceae. Strain CBA1101T had a highest 16S rRNA gene sequence similarity of 98.4 % with Halococcus dombrowskii DSM 14522T, followed by 93.7–98.3 % with sequences of other type strains in the genus Halococcus. The RNA polymerase subunit B′ gene sequence similarity of strain CBA1101T with that of Halococcus qingdaonensis JCM 13587T is 89.5 % and lower with those of other members of the genus Halococcus. Strain CBA1101T was observed to grow at 25–40 °C, pH 6.0–9.0 and in the presence of 15–30 % (w/v) NaCl, with optimal growth at 35–40 °C, pH 7.0 and with 20 % NaCl. The cells of strain CBA1101T are Gram-negative and did not lyse in distilled water. The major polar lipids were identified as phosphatidylglyerol, phosphatidylglycerol phosphate methyl ester, sulfated diglycosyl diether, unidentified phospholipids and unidentified glycolipids. The genomic DNA G+C content was determined 66.0 mol%. The DNA–DNA hybridization experiment showed that there was less than 40 % relatedness between strain CBA1101T and the reference species in the genus Halococcus. Based on this polyphasic taxonomic analysis, strain CBA1101T is considered to represent a new species in the genus Halococcus, for which the name Halococcus sediminicola sp. nov. is proposed. The type strain is CBA1101T (=JCM 18965T = CECT 8275T).  相似文献   

6.
A Gram stain-negative, aerobic and rod-shaped bacterium, strain DY22T, was isolated from a deep-sea sediment collected from the east Pacific Ocean. The isolate was found to grow in the presence of 0–20.0 % (w/v) NaCl and at pH 4.5–8.5; optimum growth was observed with 0.5–2.0 % (w/v) NaCl and at pH 5.0–7.0. Chemotaxonomic analysis showed the presence of ubiquinone-9 as predominant respiratory quinone and C16:0, C19:0 ω8c cyclo and C12:0 3-OH as major cellular fatty acids. The genomic DNA G+C content was determined to be 59.6 mol%. Comparative 16S rRNA gene sequence analysis revealed that the novel isolate belongs to the genus Salinicola. Strain DY22T exhibited the closest phylogenetic affinity to the type strain of Salinicola salarius with 97.2 % sequence similarity and less than 97 % sequence similarity with respect to other Salinicola species with validly published names. The DNA–DNA reassociation values between strain DY22T and S. salarius DSM 18044T was 52 ± 4 %. On the basis of phenotypic, chemotaxonomic and genotypic data, strain DY22T represents a novel species of the genus Salinicola, for which the name Salinicola peritrichatus sp. nov. (type strain DY22T = CGMCC 1.12381T = JCM 18795T) is proposed.  相似文献   

7.
A novel Gram-positive, motile, non-spore-forming coccus-shaped bacterial strain, designated KLBMP 1274T, was isolated from a halophytic plant (Limonium sinense) collected from the coastal region of Nantong, Jiangsu Province, in east China. Phylogenetic analyses based on the 16S rRNA gene sequence showed that strain KLBMP 1274T belongs to the genus Kineococcus and is closely related to Kineococcus rhizosphaerae RP-B16T (98.72 %), Kineococcus aurantiacus IFO 15268T (98.71 %), Kineococcus radiotolerans SRS30216T (98.69 %) and Kineococcus gynurae KKD096T (97.33 %). The 16S rRNA gene sequence similarity to other species of the genus Kineococcus was <97 %. The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid, with arabinose and galactose as the characteristic sugars. The predominant menaquinone was MK-9(H2). The polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, an unknown phospholipid, an unknown glycolipid, and three unknown lipids. Major cellular fatty acids were found to be anteiso-C15: 0 and iso-C14: 0. The chemotaxonomic data for strain KLBMP 1274T were typical of the genus Kineococcus. The total DNA G+C content was 73.4 mol %. DNA–DNA relatedness and differential phenotypic data demonstrated that strain KLBMP 1274T was clearly distinguished from all closely related species of the genus Kineococcus. Thus, strain KLBMP 1274T represents a novel species of the genus Kineococcus, for which the name Kineococcus endophytica sp. nov. is proposed. The type strain is KLBMP 1274T (=KCTC 19886 T = NBRC 108674T).  相似文献   

8.
A bacterial strain, designated M26T, was isolated from a fish gastrointestinal tract, collected from Zhanjiang Port, South China. 16S rRNA gene sequence analysis indicated that strain M26T belongs to the subclass α-Proteobacteria, being related to the genus Paracoccus, and sharing highest sequence similarity with Paracoccus alcaliphilus JCM 7364T (98.1 %), Paracoccus huijuniae FLN-7T (97.3 %), Paracoccus stylophorae KTW-16T (97.1 %) and Paracoccus seriniphilus DSM 14827T (96.9 %). The major quinone was determined to be ubiquinone Q-10, with Q-9 and Q-8 as minor components. The major fatty acid was identified as C18:1ω7c, with smaller amounts of C18:0 and C16:0. The G+C content of the genomic DNA was determined to be 64.3 mol%. The DNA hybridization value between strain M26T and the most closely related type strain, P. alcaliphilus, was 29.0 ± 1.0 %. The results of physiological and biochemical tests and low DNA–DNA relatedness showed that the strain could be readily distinguished from closely related species. On the basis of these phenotypic and genotypic data, strain M26T is concluded to represent a novel species of the genus Paracoccus, for which the name Paracoccus siganidrum sp. nov. is proposed. The type strain is M26T (=CCTCC AB 2012865T = DSM 26381T).  相似文献   

9.
A Gram-negative, coccobacilli, non-spore forming and non-motile bacterium, designated PN1T, was isolated from a banana leaf collected in Mattra island, Thailand. This isolate was observed to grow optimally at 30 °C and pH 7.0, and to grow with 0–3 % NaCl. Comparative 16S rRNA gene sequence analysis showed that strain PN1T is closely related to members of the genus Roseomonas, exhibiting the highest 16S rRNA gene sequence similarity to Roseomonas aestuarii JC17T (96.5 %). The DNA G + C content of strain PN1T was determined to be 69.7 mol %. Based on physiological and biochemical tests, and genotypic differences between strain PN1T and the validly named species of the genus Roseomonas, it is proposed that the strain be classified as a new species of Roseomonas for which the name Roseomonas musae sp. nov. is proposed. The type strain is PN1T (= BCC 44863T = NBRC 107870T).  相似文献   

10.
A novel halophilic, filamentous actinomycete, designated TRM 4064T, was isolated from a hypersaline habitat in Sichuan Province, China. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence of strain TRM 4064T showed that it was most closely related to Actinopolyspora mortivallis (99.1 % sequence similarity). The sequence similarities between strain TRM 4064T and other Actinopolyspora species with validly-published names were <97.0 %. However, it had relatively low mean values for DNA–DNA relatedness with the A. mortivallis DSM 44261T (23.2 %). Optimal growth occurred at 37 °C, pH 7.0 and in the presence of 13 % (w/v) NaCl. The whole-cell sugar pattern consists of xylose, glucose, ribose and arabinose. The predominant menaquinones are MK-10(H4) (38.2 %), MK-9(H4) (25.1 %), MK-9(H2) (28.6 %) and MK-8(H4) (7.3 %). The major fatty acids are anteiso-C17:0 (36.9 %) and iso-C17:0 (19.3 %). The diagnostic phospholipids detected were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylcholine (PC), phosphatidylinositol (PI) and two unknown phospholipids. The G+C content of the genomic DNA of the type strain is 66.3 mol%. Strain TRM 4064T therefore represents a novel species of the genus Actinopolyspora, for which the name Actinopolyspora dayingensis sp. nov. is proposed. The type strain is TRM 4064T (= KCTC 19979T = CCTCC AA 2010010T).  相似文献   

11.
A novel bacterial strain designated 9PNM-6T was isolated from an abandoned lead–zinc ore mine site in Meizhou, Guangdong Province, China. The isolate was found to be Gram-negative, rod-shaped, orange-pigmented, strictly aerobic, oxidase- and catalase-positive. Growth occurred at 0–4 % NaCl (w/v, optimum, 0 %), at pH 6.0–8.0 (optimum, pH 7.0) and at 15–32 °C (optimum, 28–30 °C). Phylogenetic analysis based on 16S rRNA gene sequence similarities showed that strain 9PNM-6T belongs to the genus Sphingomonas, with the highest sequence similarities with Sphingomonas jejuensis NBRC 107775T (99.7 %), Sphingomonas koreensis KCTC 2882T (95.1 %) and Sphingomonas dokdonesis KCTC 12541T (95.1 %). The chemotaxonomic characteristics of strain 9PNM-6T were consistent with those of the genus Sphingomonas. The predominant respiratory quinone was identified as ubiquinone Q-10, the major polyamine as sym-homospermidine, and the major cellular fatty acids as C18:1 ω7c, C16:0, C16:1 ω7c and/or C16:1 ω6c and C14:0 2-OH. The major polar lipids are sphingoglycolipid, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatideylcholine, an unidentified phospholipid and four unidentified aminolipids. The genomic DNA G+C content of strain 9PNM-6T was determined to be 69.2 ± 0.6 mol%. Based on comparative analyses of morphological, physiological and chemotaxonomic data, and levels of DNA–DNA relatedness values, strain 9PNM-6T is considered to represent a novel species of the genus Sphingomonas, for which the name Sphingomonas gimensis sp. nov. (Type strain 9PNM-6T = GIMCC 1.655T = CGMCC 1.12671T = DSM 27569T) is proposed.  相似文献   

12.
The strain NSC3T, a novel, facultative, chemolithotrophic, denitrifying, alkaliphilic, sulfide-oxidizing bacterium isolated from a hot spring in Yang-Ming Mountain, Taiwan, was Gram negative, rod shaped, and motile by single polar flagella and grew facultatively by adopting a denitrifying metabolism. The 16S rRNA sequence analysis revealed that strain NSC3T belongs to beta subclass of the Proteobacteria and most closely related to Azoarcus evansii KB740T (95.44 %), Azoarcus toluvorans Td-21T (95.21 %), Azoarcus tolulyticus Tol-4T (95.08 %), and Azoarcus toluclasticus MF63T (94.94 %). The phylogenetic analyses based on 16S rRNA gene sequences indicated that the strain NSC3T formed a distinct lineage in the Betaproteobacteria and that it exhibited the highest level of sequence similarity with species of the genera Azoarcus (95.28–93.13 %). The major fatty acids of the type strain were C16:0 (26.9 %), C16:1w7c (28.9 %), C18:0 (9.6 %), and C18:1w7c/w6c (29.9 %). The DNA G+C content of genomic DNA was 63.7 mol%. On the basis of the 16S rRNA sequence similarity, phenotypic and genotypic characteristics, and chemotaxonomic data, the strain NSC3T could be differentiated from other species of the genus Azoarcus. Therefore, strain NSC3T (equal to BCRC 80111T and DSM 24109T) is proposed as a novel species in genus Azoarcus, for which the name Azoarcus taiwanensis sp. nov. is proposed. The strain NSC3T is deposited in Bioresource Collection and Research Center, Taiwan, under the reference number BCRC 80111T, and German Collection of Microorganisms and Cell Cultures, Germany (DSMZ), with DSM 24109T.  相似文献   

13.
A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 6Alg 8T, was isolated from the common Pacific green alga Ulva fenestrata. The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 97.6 % to Polaribacter dokdonensis DSW-5T and 92.8–96.1 % to other recognized Polaribacter species. The prevalent fatty acids of strain 6Alg 8T were iso-C15:0, iso-C15:1, iso-C15:0 2-OH, C15:0 and C15:1ω6. The polar lipid profile consisted of the major lipids phosphatidylethanolamine, two unknown aminolipids and one unknown lipid. The DNA G+C content of the type strain is 31.6 mol%. The new isolate and the type strains of recognized species of the genus Polaribacter were readily distinguished based on a number of phenotypic characteristics. A combination of the genotypic and phenotypic data showed that the algal isolate represents a novel species of the genus Polaribacter, for which the name Polaribacter reichenbachii sp. nov. is proposed. The type strain is 6Alg 8T (= KCTC 23969T = KMM 6386T = LMG 26443T).  相似文献   

14.
A Gram-negative, aerobic, short-rod-shaped, non-motile bacterium designated Rs7T, was isolated from peat soil collected from Russia and was characterized to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that the strain Rs7T belongs to the class Betaproteobacteria. The highest degree of sequence similarities were determined to be with Burkholderia tropica Ppe8T (98.4 %), Burkholderia unamae MTI-641T (97.8 %), Burkholderia bannensis E25T (97.7 %), Burkholderia heleia SA41T (97.0 %), and Burkholderia sacchari IPT101T (97.0 %). Chemotaxonomic data revealed that the strain Rs7T possesses ubiquinone Q-8. The polar lipid profile of strain Rs7T contained phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, and an unknown amino phospholipid. The predominant fatty acids were C16:0, C19:0 cyclo ω8c, and C17:0 cyclo, all of which corroborated the assignment of the strain to the genus Burkholderia. The DNA G+C content was 63.2 mol%. DNA–DNA hybridization experiments showed less than 37.8 % DNA relatedness with closely related type strains, thus confirming separate species status. The results of physiological and biochemical tests allowed phenotypic differentiation of strain Rs7T from the members of the genus Burkholderia. Based on these data, Rs7T (=KEMC 7302-068T = JCM 18069T) should be classified as the type strain for a novel Burkholderia species, for which the name Burkholderia humi sp. nov. is proposed.  相似文献   

15.
A Gram-negative, oxidase-positive, facultatively anaerobic bacterium, designated strain E20121, was isolated from the digestive tract of a Japanese prawn (Marsupenaeus japonicus) collected from the coastal sea water area of Zhuhai, Guangdong province, China. The new isolate was determined to be closely related to Vibrio ponticus DSM 16217T, having 97.6 % 16S rRNA gene sequence similarity. Phylogenetic analysis based on recA, pyrH and rpoA also showed low levels of sequence similarities (72.6–96.6 %) with all species of the genus Vibrio. A multigene phylogenetic tree using concatenated sequences of the four genes (16S rRNA, rpoA, recA and pyrH) clearly showed that the new isolate is different from the currently known Vibrio species. DNA–DNA hybridization experiments revealed similarity values below 70 % with the closest related species V. ponticus DSM 16217T. Several phenotypic traits enabled the differentiation of strain E20121 from the closest phylogenetic neighbours. The DNA G+C content of strain E20121 was determined to be 47.6 mol % and the major fatty acid components identified were C16:1ω7c and/or C16:1ω6c (39.8 %), C18:1ω7c (13.6 %) and C16:0 (9.6 %). Based on genotypic, phenotypic, chemotaxonomic, phylogenetic and DNA–DNA hybridization analyses, strain E20121 is proposed to represent a novel species of the genus Vibrio for which the name Vibrio zhuhaiensis sp. nov. is proposed. The type strain is E20121T(=DSM 25602T = CCTCC AB 2011174T).  相似文献   

16.
A rod-shaped actinobacterium, designated Sco-A25T, was isolated from a red-coloured layer of scoria (volcanic ash) in the Republic of Korea and subjected to a polyphasic taxonomic characterization. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain Sco-A25T is a member of the genus Nocardioides and formed a tight monophyletic unit with the type strain of Nocardioides plantarum (98.7 % gene similarity). LL-Diaminopimelic acid was detected in the cell wall. The predominant menaquinone is MK-8(H4). The polar lipids found were phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol, and an unknown phospholipid. The fatty acid profile was represented by large amounts of saturated, unsaturated and iso-branched fatty acids. The DNA G+C content was 71.6 mol %. Genomic DNA similarity between strain Sco-A25T and N. plantarum KCTC 9577T was 47.8 %. On the basis of the results of phenotypic, genetic and phylogenetic analyses presented here, strain Sco-A25T is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides rubroscoriae sp. nov. is proposed. The type strain is Sco-A25T (=KCTC 19805T = DSM 23986T = NBRC 107916T).  相似文献   

17.
A novel actinomycete strain, designated VRC07T, was isolated from a Callistemon citrinus rhizosphere sample collected from Hyderabad, India. Its taxonomic status was determined by using polyphasic approach. It is a Gram-positive, aerobic, non-motile, weakly acid-fast strain. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain VRC07T is a member of the genus Nocardia. The highest levels of 16S rRNA gene sequence similarity was found between the strains Nocardia niwae W9241T (99.6 %), Nocardia amikacinitolerans W9988T (99.3 %) and Nocardia arthritidis IFM 10035T (98.9 %); similarity to other type strains of the genus Nocardia was below 98.7 %. The organism had chemical and morphological features consistent with its classification in the genus Nocardia such as meso-diaminopimelic acid as the diagnostic diamino acid in the cell wall peptidoglycan. Arabinose and galactose as the diagnostic sugars. Diagnostic polar lipids were phosphatidylinositol, diphosphatidylglycerol, and phosphatidylglycerol. The predominant menaquinone was MK-8(H4, ω-cycl). The major fatty acids were C16:0, C18:0, C18:1 w9c, C18:0 10-methyl TBSA and sum in feature 3 (16:1 w7c/16:1 w6c). The G+C content of the genomic DNA was 68.5 mol%. The DNA–DNA relatedness data, together with phenotypic differences clearly distinguished the isolate from its closest relatives. On the basis of these phenotypic and genotypic data, the isolate represents a novel species, for which the name Nocardia bhagyanesis sp. nov., is proposed. The type strain is VRC07T (=KCTC 29209T = MTCC 11725T = ATCC BAA-2548).  相似文献   

18.
A novel Gram-stain-positive, slightly halophilic, catalase- and oxidase-positive, endospore-forming, motile, aerobic, rod-shaped bacterium, designated strain JSM 081003T, was isolated from non-saline forest soil in Hunan Province, China. Growth occurred with 0.5?C15% (w/v) NaCl (optimum 2?C4%) at pH 6.5?C10.5 (optimum pH 7.5?C8.5) and at 5?C40°C (optimum 30°C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The major cellular fatty acids were iso-C15:0, anteiso-C15:0 and iso-C14:0. Strain JSM 081003T contained MK-7 as the predominant respiratory quinone, and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol as the major polar lipids. The genomic DNA G + C content of strain JSM 081003T was 40.9 mol%. A phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 081003T should be assigned to the genus Bacillus, and was related most closely to the type strains of Bacillus lehensis (sequence similarity 99.6%), Bacillus oshimensis (99.4%) and Bacillus patagoniensis (96.6%); lower than 96.0% sequence similarity was observed with other Bacillus species. The combination of phylogenetic analysis, DNA?CDNA relatedness values, phenotypic characteristics and chemotaxonomic data supports the view that strain JSM 081003T represents a new species of the genus Bacillus, for which the name Bacillus hunanensis sp. nov. is proposed. The type strain is JSM 081003T (= DSM 23008T = KCTC 13711T).  相似文献   

19.
Gram-negative, free-living bacterial strain ptl-3T was isolated from Himalayan valley soil, India. Polyphasic taxonomy was performed including morphological characterization, fatty acid analysis, biochemical tests, 16S rRNA and nifH gene sequence analyses. 16S rRNA gene sequence analysis showed that the strain ptl-3T belonged to the genus Azospirillum and was closely related to A. brasilense (98.7 % similarity) and A. rugosum (97 % similarity). 16S rRNA gene sequence similarity (96–95 %) was shown with other members of the genus Azospirillum. Major fatty acid 18:1ω7c was also similar to the genus Azospirillum. DNA–DNA relatedness value between strain ptl-3T and A. brasilense was found to be 47 %. Various biochemical tests showed that the strain ptl-3T differed from its closely related species A. brasilense. On the basis of phenotypic, chemotaxonomic and molecular genetics evidence, a bacterium with the type strain ptl-3T is proposed as a novel species of the genus Azospirillum. The name of bacterial strain ptl-3T has been proposed as Azospirillum himalayense sp. nov. The type strain of ptl-3T (CCUG 58760T, KCTC 23189T) has been submitted to two culture collection centres. The accession numbers for 16S rRNA and nifH gene are GQ 284588 and GQ 249665. respectively.  相似文献   

20.
A novel actinomycete, designated strain NEAU-GH7T, was isolated from a lake sediment and characterized using a polyphasic approach. Strain NEAU-GH7T was Gram-stain positive, aerobic, non-spore-forming and produced spherical sporangia. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-GH7T formed a monophyletic clade with the closest relative Streptosporangium longisporum DSM 43180T (99.0 %), an association that was supported by a bootstrap value of 74 % in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. However, the low level of DNA–DNA relatedness allowed the strain to be differentiated from its closest relative. Moreover, strain NEAU-GH7T could also be differentiated from S. longisporum DSM 43180T and other Streptosporangium species showing high 16S rRNA gene sequence similarity (>98.0 %) by morphological and physiological characteristics. On the basis of phylogenetic analysis, DNA–DNA hybridization and phenotypic characteristics, strain NEAU-GH7T should be classified as a new species of the genus Streptosporangium, for which the name Streptosporangium shengliensis sp. nov. is proposed. The type strain is NEAU-GH7T (=CGMCC 4.7105T=DSM 45881T).  相似文献   

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