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1.
Summary. Recent literature suggests that both caffeine and taurine can induce diuresis and natriuresis in rat and man. Although they
act via different cellular mechanisms, their diuretic actions might be additive. This is of considerable interest, as several
commercially available energy drinks contain both substances.
In this study we examined the possible diuretic effects of caffeine and taurine in a cross-over-design in which 12 healthy
male volunteers received each of 4 different test drinks (750 ml of energy drink containing 240 mg caffeine and 3 g taurine,
the three other test drinks either lacked caffeine, taurine or both) after restraining from fluids for 12 h.
Mixed model analyses demonstrated that urinary output and natriuresis were significantly increased by caffeine (mean differences
243 ml and 27 mmol; both p < 0.001) and that there were no such effects of taurine (mean differences 59 ml and −4 mmol). Additionally, urinary osmolarity
at baseline was significantly related to the urinary output (p < 0.001). Urine osmolarity values at baseline and in the 6 h urine collection did not differ significantly between treatments.
Taken together, our study demonstrates that diuretic and natriuretic effects of the tested energy drink were largely mediated
by caffeine. Taurine played no significant role in the fluid balance in moderately dehydrated healthy young consumers. Consequently,
the diuretic potential of energy drinks will not differ significantly from other caffeine containing beverages. 相似文献
2.
Summary. The aim of the present study was to measure MPO activity in PMN leukocytes after endotoxin administration, and to compare
the levels of NO2
− competing with taurine for reaction with HOCl. Furthermore we aimed to determine TauCl levels, a product of MPO–H2O2–Halide system, and to evaluate anti-inflammatory properties of PMN in endotoxemia. In addition, our second objective was
to investigate the effect of taurine, an antioxidant amino acid, on anti-bactericidal and anti-inflammatory functions of PMN
after administration of endotoxin together with taurine.
All experiments were performed with four groups (control, taurine, endotoxemia, and taurine plus endotoxin) of ten guinea
pigs. After endotoxin administration (4 mg/kg), MPO activities increased and taurine levels decreased. Therefore levels of
TauCl, NO2
•− increased. We observed the effects of taurine as conflicting. When taurine was administrated alone (300 mg/kg), all of these
parameters decreased.
Consequently, we suggested that taurine is influential in infected subjects but not on healthy ones as an antioxidative amino
acid. In addition, we believe that in vivo effects of taurine may differ from those in vitro depending on its dosage. 相似文献
3.
Guz G Oz E Lortlar N Ulusu NN Nurlu N Demirogullari B Omeroglu S Sert S Karasu C 《Amino acids》2007,32(3):405-411
Summary. Ischemia-reperfusion (I/R) injury is one of the most common causes of renal dysfunction. Taurine is an endogenous antioxidant
and a membrane-stabilizing, intracellular, free beta-amino acid. It has been demonstrated to have protective effects against
I/R injuries to tissues other than kidney. The aim of this study was to determine whether taurine has a beneficial role in
renal I/R injury. Forty Wistar-Albino rats were allocated into four groups as follows: sham, taurine, I/R, and I/R + taurine.
Taurine 7.5 mg/kg was given intra-peritoneally to rats in the groups taurine and I/R + taurine. Renal I/R was achieved by
occluding the renal arteries bilaterally for 40 min, followed by 6 h of reperfusion. Immediately thereafter, blood was drawn
and tissue samples were harvested to measure 1) serum levels of BUN and creatinine; 2) serum and/or tissue levels of malondialdehyde
(MDA), glutathione (GSH), glucose 6-phosphate dehydrogenase (G-6PD), 6-phosphogluconate dehydrogenase (6-PGD) and glutathione
reductase (GSH-red); 3) renal morphology; and 4) immunohistochemical staining for P-selectin. Taurine administration reduced
I/R-induced increases in serum BUN and creatinine, and serum and tissue MDA levels (p < 0.05). Additionally, taurine lessened
the reductions in serum and tissue glutathione levels secondary to I/R (p < 0.05). Taurine also attenuated histopathologic
evidence of renal injury, and reduced I/R-induced P-selectin immunoreactivity (p < 0.05). Overall, then, taurine administration
appears to reduce the injurious effects of I/R on kidney. 相似文献
4.
Summary. The aim of this study was to evaluate the effect of endotoxin on PMN leukocyte respiratory burst activity by measuring G6PD,
NADPH oxidase and XO activities in guinea pig. In addition, the possible protective role of taurine against endotoxin-mediated
PMN leukocyte function was examined. All experiments were performed with four groups (control, taurine, endotoxemia, taurine
plus endotoxin) of ten guinea pigs. After the endotoxin was administrated (4 mg/kg) both G6PD and NADPH oxidase activities
were significantly reduced compared with the control group. NADPH oxidase activity returned to the control value and G6PD
activity also increased but it did not reach the control value. However when taurine was administrated (300 mg/kg) the activity
of NADPH oxidase reached the control value; furthermore, G6PD activity also increased but it could not reach to the control
value. When taurine was administrated alone, no effect on these enzymes was observed. Following the endotoxin administration,
the activity of XO considerably increased. When taurine was administrated together with endotoxine and alone, this activity
decreased compared to control value in both conditions. These results indicate that the O2
•− formation in PMN leukocytes after the endotoxin administration is ensured by the catalysis of XO due to the inhibited NADPH
oxidase activity. It was observed that taurine has considerable anti-inflammatory and antioxidant effects. However, conflicting
results were obtained when taurine was administrated alone or together with an oxidant agent. 相似文献
5.
Baran H 《Amino acids》2006,31(3):303-307
Summary. The aim of the study was to investigate the changes of taurine in the kainic acid (KA, 10 mg/kg, s.c.) chronic model of epilepsy,
six months after KA application. The KA-rats used were divided into a group of animals showing weak behavioural response to
KA (WDS, rare focal convulsion; rating scale <2 up to 3 h after KA injection) and a group of strong response to KA (WDS, seizures;
rating >3 up to 3 h after KA injection). The brain regions investigated were caudate nucleus, substantia nigra, septum, hippocampus,
amygdala/piriform cortex, and frontal, parietal, temporal and occipital cortices. KA-rats with rating <2 developed spontaneous
WDS which occurred chronically and six months after KA injection increased taurine levels were found in the hippocampus (125.4%
of control). KA-rats with rating >3 developed spontaneous recurrent seizures and six months after injection increased taurine
levels were found in the caudate nucleus (162.5% of control) and hippocampus (126.6% of control), while reduced taurine levels
were seen in the septum (78.2% of control). In summary, increased taurine levels in the hippocampus may involve processes
for membrane stabilisation, thus favouring recovery after neuronal hyperactivity. The increased taurine levels in the caudate
nucleus could be involved in the modulation of spontaneous recurrent seizure activity. 相似文献
6.
Summary. Taurine as well as tauret (retinyliden taurine) levels were measured in locust Locusta migratoria compound eyes. HPLC measurements revealed relatively low taurine levels (1.9 ± 0.16 mM) in dark-adapted eyes. Glutamate,
aspartate and glycine levels were 2.0 ± 0.2, 2.7 ± 0.4 and 3.0 ± 0.37 mM, respectively, while GABA was present only in trace
amounts. After about 4 h of light adaptation at 1500–2000 lx, amino acid levels in the compound eye were as follows: taurine,
1.8 ± 0.17 mM; glutamate, no change at 2.1 ± 0.2 mM; aspartate sharply increased to 4.7 ± 0.7 mM; glycine slightly decreased
to 2.8 ± 0.3 mM; and GABA trace levels. In the compound eye of locust Locusta migratoria, the existence of endogenous tauret in micro-molar range was established. In the dark, levels were several times higher compared
with compound eye after light adaptation 1500 lx for 3 h, as estimated by TLC in combination with spectral measurements. Existence
of tauret in compound eye is of special interest because in the compound eye, rhodopsin regeneration is based on photoregeneration. 相似文献
7.
Summary. Taurine has been reported to enhance cholesterol 7α-hydroxylase (CYP7A1) mRNA expression in animal models. However, no in vitro studies of this effect have been reported. The Hep G2 human hepatoma cell line has been recognized as a good model for studying
the regulation of human CYP7A1. This work characterizes the effects of taurine on CYP7A1 mRNA levels of Hep G2 cells in a
dose- and time-dependent manner. In the dose-dependent experiment, Hep G2 cells were treated with 0, 2, 10 or 20 mM taurine
in the presence or absence of cholesterol 0.2 mM for 48 h. In the time-dependent experiment, Hep G2 cells were treated with
0 or 20 mM taurine for 4, 24 and 48 h with and without cholesterol 0.2 mM. Our data revealed that taurine showed time- and
dose-response effects on CYP7A1 mRNA levels in Hep G2 cells. However, glycine – a structural analogue of taurine – did not
have an effect on CYP7A1 gene expression. These results show that, in agreement to previous studies on animal models, taurine
induces the mRNA levels of CYP7A1 in Hep G2 cells, which could enhance cholesterol conversion into bile acids. Also, Hep G2
cell line may be an appropriate model to study the effects of taurine on human cholesterol metabolism. 相似文献
8.
Summary. Glutathione (reduced form GSH and oxidized form GSSG) constitutes an important defense against oxidative stress in the brain,
and taurine is an inhibitory neuromodulator particularly in the developing brain. The effects of GSH and GSSG and glycylglycine,
γ-glutamylcysteine, cysteinylglycine, glycine and cysteine on the release of [3H]taurine evoked by K+-depolarization or the ionotropic glutamate receptor agonists glutamate, kainate, 2-amino-3-hydroxy-5-methyl-4-isoxazolepropionate
(AMPA) and N-methyl-D-aspartate (NMDA) were now studied in slices from the hippocampi from 7-day-old mouse pups in a perfusion system.
All stimulatory agents (50 mM K+, 1 mM glutamate, 0.1 mM kainate, 0.1 mM AMPA and 0.1 mM NMDA) evoked taurine release in a receptor-mediated manner. Both
GSH and GSSG significantly inhibited the release evoked by 50 mM K+. The release induced by AMPA and glutamate was also inhibited, while the kainate-evoked release was significantly activated
by both GSH and GSSG. The NMDA-evoked release proved the most sensitive to modulation: L-Cysteine and glycine enhanced the
release in a concentration-dependent manner, whereas GSH and GSSG were inhibitory at low (0.1 mM) but not at higher (1 or
10 mM) concentrations. The release evoked by 0.1 mM AMPA was inhibited by γ-glutamylcysteine and cysteinylglycine, whereas
glycylglycine had no effect. The 0.1 mM NMDA-evoked release was inhibited by glycylglycine and γ-glutamylcysteine. In turn,
cysteinylglycine inhibited the NMDA-evoked release at 0.1 mM, but was inactive at 1 mM. Glutathione exhibited both enhancing
and attenuating effects on taurine release, depending on the glutathione concentration and on the agonist used. Both glutathione
and taurine act as endogenous neuroprotective effectors during early postnatal life.
Authors’ address: Prof. Simo S. Oja, Brain Research Center, Medical School, FI-33014 University of Tampere, Finland 相似文献
9.
Sved DW Godsey JL Ledyard SL Mahoney AP Stetson PL Ho S Myers NR Resnis P Renwick AG 《Amino acids》2007,32(4):459-466
Summary. Three biodisposition studies with taurine were performed in male and female adult rats at dosages of 30 and 300 mg/kg. A single
oral dose of 14C-taurine was rapidly absorbed, distributed to tissues and excreted unchanged in urine. Elimination of radioactivity from
intracellular pools was slow. Pre-treatment of animals for 14 days with unlabelled taurine did not significantly affect the
fate of 14C-taurine. At the higher dose there was more extensive excretion combined with a lower percentage of the dose in the carcass,
indicating the possibility of saturation of the tubular reabsorption mechanism for taurine. Daily administration of unlabelled
taurine for 14 days did not result in an increase in total taurine in the brain. The data indicate that exogenous taurine
rapidly equilibrates with endogenous body pools and that any excess is rapidly eliminated by the kidneys. 相似文献
10.
Summary. The main objective of the present study was to evaluate the in vivo and in vitro effect of Arg on serum nucleotide hydrolysis. The action of Nω-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide synthase, on the effects produced by Arg was also examined.
Sixty-day-old rats were treated with a single or a triple (with an interval of 1 h between each injection) intraperitoneal
injection of saline (group I), Arg (0.8 g/kg) (group II), L-NAME (2.0 mg/kg or 20 mg/kg) (group III) or Arg (0.8 g/kg) plus
L-NAME (2.0 mg/kg or 20 mg/kg) (group IV) and were killed 1 h later. The present results show that a triple Arg administration
decreased ATP, ADP and AMP hydrolysis. Simultaneous injection of L-NAME (20 mg/kg) prevented such effects. Arg in vitro did not alter nucleotide hydrolysis. It is suggested that in vivo Arg administration reduces nucleotide hydrolysis in rat serum, probably through nitric oxide or/and peroxynitrite formation.
Both are first authors. 相似文献
11.
Summary. The purpose of this study was to determine whether the γ-aminobutyric acid (GABA) affects the rate of brain protein synthesis
in male rats. Two experiments were done on five or three groups of young rats (5 wk) given the diets containing 20% casein
administrated 0 mg, 25 mg, 50 mg, 100 mg or 200 mg/100 g body weight GABA dissolved in saline by oral gavage for 1 day (d)
(Experiment 1), and given the diets contained 0%, 0.25% or 0.5% GABA added to the 20% casein diet (Experiment 2) for 10 d.
The plasma concentration of growth hormone (GH) was the highest in rats administrated 50 mg and 100 mg/100 g body weight GABA.
The concentration of serum GABA increased significantly with the supplementation groups. The fractional (Ks) rates of protein
synthesis in brain regions, liver and gastrocnemius muscle increased significantly with the 20% casein + 0.25% GABA diet and
still more 20% casein + 0.5% GABA compared with the 20% casein diet. In brain regions, liver and gastrocnemius muscle, the
RNA activity [g protein synthesized/(g RNA·d)] significantly correlated with the fractional rate of protein synthesis. The
RNA concentration (mg RNA/g protein) was not related to the fractional rate of protein synthesis in any organ. Our results
suggest that the treatment of GABA to young male rats are likely to increase the concentrations of plasma GH and the rate
of protein synthesis in the brain, and that RNA activity is at least partly related to the fractional rate of brain protein
synthesis. 相似文献
12.
Taranukhin AG Taranukhina EY Saransaari P Djatchkova IM Pelto-Huikko M Oja SS 《Amino acids》2008,34(1):169-174
Summary. Taurine is a sulphur-containing amino acid abundant in the nervous system. It protects cells from ischemia-induced apoptosis,
but the mechanism underlying this is not well established. The aim of our study was to explore the effects of taurine on two
main pathways of apoptosis induced by ischemia: receptor-mediated and mitochondrial cell death. Brain slices containing the
supraoptic (SON) and paraventricular (PVN) nuclei of the hypothalamus were incubated in vitro under control and simulated
ischemic (oxygen-glucose deprivation for 30 min) conditions in the absence and presence of 20 mM taurine. Brain slices were
harvested after the 180-min “postischemic” period and fixed in 4% paraformaldehyde. To estimate apoptosis, immunostaining
was done for caspase-8 and caspase-9 in paraffin-embedded sections. Immunoreactive caspase-8 and caspase-9 cells were observed
in SON and PVN in all experimental groups, but in the “ischemic” group the expression of caspase-8 and caspase-9 and the number
of immunoreactive cells was significantly increased in both hypothalamic nuclei. Addition of taurine (20 mM) to the incubation
medium induced a marked decrease in caspase-8 and caspase-9 immunoreactivity after ischemia in SON and PVN when compared with
the taurine-untreated “ischemic” group. Taurine reduces ischemia-induced caspase-8 and caspase-9 expression, the key inductors
of apoptosis in SON and PVN.
Authors’ address: Dr. Andrey Taranukhin, Tampere Brain Research Center, Medical School, University of Tampere, FI-33014 Finland 相似文献
13.
Summary. Caffeine- and taurine-containing drinks have been on the European market for about a decade, and research on the individual
constituents of these drinks indicates an improvement in cognitive performance resulting from consumption of such drinks.
In this double-blind, placebo-controlled study using 10 graduate students, we obtained the P300 components of event-related
potential (ERP) waveforms following an auditory oddball paradigm, measured motor reaction time, and applied the d2 test for
the assessment of attention. Status of mood was assessed by the “Basler-Befindlichkeitsbogen” questionnaire, a standard test
for evaluation of feelings of well-being. Measurements were made at night, prior to and starting one hour after consumption
of energy drink ingredients or placebo.
At the end of the experiment (midnight), P300 latency and motor reaction time were significantly longer compared with baseline
measurements in the placebo group, but were unchanged in the energy drink group. In the test system for evaluating feelings
of well-being, total scores, vitality scores and social extrovertedness scores were significantly decreased in the placebo
group but not in the energy drink group.
The findings clearly indicate that the mixture of three key ingredients of Red BullR Energy Drink used in the study (caffeine, taurine, glucuronolactone) have positive effects upon human mental performance
and mood. These effects may be mediated by the action of caffeine on purinergic (adenosinergic) receptors and taurine modulation
of receptors. As half of the study cohort were non-caffeine users, the described effects cannot be explained in terms of the
restoration of plasma caffeine levels to normal following caffeine withdrawal.
Received January 5, 2000/Accepted June 5, 2000 相似文献
14.
El Idrissi A 《Amino acids》2008,34(2):321-328
Summary. We have determined the role of mitochondria in the sequestration of calcium after stimulation of cerebellar granule cells
with glutamate. In addition we have evaluated the neuroprotective role of taurine in excitotoxic cell death. Mitochondrial
inhibitors were used to determine the calcium buffering capacity of mitochondria, as well as how taurine regulates the ability
of mitochondria to buffer intracellular calcium during glutamate depolarization and excitotoxicity. We report here that pre-treatment
of cerebellar granule cells with taurine (1 mM, 24 h) significantly counteracted glutamate excitotoxicity. The neuroprotective
role of taurine was mediated through regulation of cytoplasmic free calcium ([Ca2+]
i
), and intra-mitochondrial calcium homeostasis, as determined by fluo-3 and 45Ca2+-uptake. Furthermore, the overall mitochondrial function was increased in the presence of taurine, as assessed by rhodamine
accumulation into mitochondria and total cellular ATP levels. We specifically tested the hypothesis that taurine reduces glutamate
excitotoxicity through both the enhancement of mitochondrial function and the regulation of intracellular (cytoplasmic and
intra-mitochondrial) calcium homeostasis. The role of taurine in modulating mitochondrial calcium homeostasis could be of
particular importance under pathological conditions that are characterized by excessive calcium overloads. Taurine may serve
as an endogenous neuroprotective molecule against brain insults.
Authors’ address: Abdeslem El Idrissi, Biology Department and Center for Developmental Neuroscience, College of Staten Island/CUNY,
6S-134 Staten Island, NY 10314, U.S.A. 相似文献
15.
Summary. Increased levels in plasma homocysteine and cysteine, and more recently, decreased levels in cysteinylglycine have been indicated
as a risk factor for vascular diseases. Most assays focused their attention only on homocysteine determination and when also
other thiols were measured, analytical times drastically increased. By modifying our previous method for thiols detection,
we set up a rapid capillary electrophoresis method for the selective quantification of plasma cysteinylglycine, cutting the
analysis time of about 50%. Samples were treated with tri-n-butylphosphine as reducing agent, proteins were precipitated with
trichloroacetic acid and released thiols were successively derivatized by the selective thiol laser-induced fluorescence-labeling
agent 5-iodoacetamidofluorescein and separated by capillary electrophoresis. A baseline separation between peaks was obtained
in about 2 min using 3 mmol/L sodium phosphate/2.5 mmol/L boric acid as electrolyte solution with 75 mmol/L N-methyl-D-glucamine
at pH 11.25 in a 47 cm long capillary with a cartridge temperature of 45 °C. The method application was checked by measuring
plasma Cys-Gly levels in a group of patients affected by retinal vein occlusion (RVO), an important cause of visual loss in
the elderly. The low levels of Cys-Gly found in the RVO patients suggest that these small thiols may have importance in the
disease development.
Authors’ addresses: Dr. Angelo Zinellu, Dr. Ciriaco Carru, Department Biomedical Sciences, Chair of Clinical Biochemistry,
University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy 相似文献
16.
Summary. Goldfish retinal explant outgrowth in the presence of fetal calf serum is stimulated by taurine. In the absence of it, but
with glucose in the medium, length of neurites is still elevated by the amino acid. Using the medium in the presence of glucose,
but in the absence of fetal calf serum, we explored the effect of optic tectum medium from cultures of them coming from goldfish
without crush of the optic nerve or 3, 5, 10, 14 and 20 days after crush. Retinal explants, intact or from goldfish with crush
of the optic nerve 10 days prior to starting the culture, were employed in order to measure the possible effect of optic tectum
media and the inter action with taurine. In other type of experiments the optic nerve was crushed 1, 2, 4, 7 and 10 days before
dissection of the optic tectum, and then co-cultured with intact or 10 days post-crush retinal explants. Optic tectum media
produced a time-dependent effect on outgrowth in lesioned retinas with a maximum effect around 5 days after the lesion for
the corresponding optic tectum. Taurine, 4 mM, did not further affect the outgrowth in the presence of optic tectum media,
but did significantly increase length of neurites either in intact or in post-lesion retinas. Co-culture of optic tectum at
different days post-lesion and retinas at 10 days post-lesion increased the outgrowth around 4 days post-lesion, in a preparation
resulting in mutual effects of both types of tissues. The addition of taurine in these conditions did not further increase
outgrowth, rather inhibited it according to the time after lesion of optic nerve corresponding to the co-cultured optic tectum.
The effect of taurine was concentration-dependent, since 0.2 mM was more effective than 2 or 4 mM in the presence of optic
tectum with lesion of 2 days. These results demonstrate the time-course of the regeneration processes in the visual system
of goldfish, indicating the crucial periods after crush in which the tectum could produce stimulation and later decrease or
no effect on outgrowth from the retina. In addition, they are evidences of the interaction between taurine and optic tectum
production of time-produced specific agents. The mechanisms underlying these effects are closely related to calcium, as it
was demonstrated by the addition of extracellular or intracellular chelators to the medium, which inhibited the effects of
the optic tectum and the trophic properties of taurine in this system. The inhibitor of taurine transport, guanidoethylsulfonate,
also decreased the stimulatory effects of the optic tectum and of taurine, indicating an interaction of substances produced
by the tectum with taurine, and an effect of taurine mediated through its entrance to the cells. Overall, retinal explants
outgrowth in the absence of fetal calf serum, the interaction of agents of the optic tectum and taurine modulates outgrowth
from the retina, and these effects are mediated by calcium levels and by the levels of intracellular taurine. 相似文献
17.
Garcia RF Gazola VA Barrena HC Hartmann EM Berti J Toyama MH Boschero AC Carneiro EM Manso FC Bazotte RB 《Amino acids》2007,33(1):151-155
Summary. Our purpose was to determine the blood amino acid concentration during insulin induced hypoglycemia (IIH) and examine if the
administration of alanine or glutamine could help glycemia recovery in fasted rats. IIH was obtained by an intraperitoneal
injection of regular insulin (1.0 U/kg). The blood levels of the majority of amino acids, including alanine and glutamine
were decreased (P < 0.05) during IIH and this change correlates well with the duration than the intensity of hypoglycemia. On the other hand,
the oral and intraperitoneal administration of alanine (100 mg/kg) or glutamine (100 mg/kg) accelerates glucose recovery.
This effect was partly at least consequence of the increased capacity of the livers from IIH group to produce glucose from
alanine and glutamine. It was concluded that the blood amino acids availability during IIH, particularly alanine and glutamine,
play a pivotal role in recovery from hypoglycemia. 相似文献
18.
Maize (Zea mays L.) cell cultures incorporated radioactivity from [14C]cinnamate into hydroxycinnamoyl-CoA derivatives and then into polysaccharide-bound feruloyl residues. Within 5–20 min, the
CoA pool had lost its 14C by turnover and little or no further incorporation into polysaccharides then occurred. The system was thus effectively a
pulse–chase experiment. Kinetics of radiolabelling of diferulates (also known as dehydrodiferulates) varied with culture age.
In young (1–3 d) cultures, polysaccharide-bound [14C]feruloyl- and [14C]diferuloyl residues were both detectable within 1 min of [14C]cinnamate feeding. Thus, feruloyl residues were dimerised <1 min after their attachment to polysaccharides. For at least
the first 2.3 h after [14C]cinnamate feeding, polysaccharide-bound [14C]diferuloyl residues remained almost constant at ≈7% of the total polysaccharide-bound [14C]ferulate derivatives. Since feruloyl residues are attached to polysaccharides <1 min after the biosynthesis of the latter,
and >10 min before secretion, the data show that extensive feruloyl coupling occurred intra-protoplasmically. Exogenous H2O2 (1 mM) caused little additional feruloyl coupling; therefore, wall-localised coupling may have been peroxidase-limited. In
older (e.g. 4 d) cultures, less intraprotoplasmic coupling occurred: during the first 2.5 h, polysaccharide-bound [14C]diferuloyl residues were a steady 1.4% of the total polysaccharide-bound [14C]ferulate derivatives. In contrast to the situation in younger cultures, exogenous H2O2 induced a rapid 4- to 6-fold increase in all coupling products, indicating that coupling in the walls was H2O2-limited. In both 2- and 4-d-old cultures, polysaccharide-bound 14C-trimers and larger coupling products exceeded [14C]diferulates 3- to 4-fold, but followed similar kinetics. Thus, although all known dimers of ferulate can now be individually
quantified, it appears to be trimers and larger products that make the major contribution to cross-linking of wall polysaccharides
in cultured maize cells. We argue that feruloyl arabinoxylans that are cross-linked before and after secretion are likely
to loosen and tighten the cell wall, respectively. The consequences for the control of cell expansion and for the response
of cell walls to an oxidative burst are discussed.
Received: 19 January 2000 / Accepted: 13 April 2000 相似文献
19.
Summary. Nitric oxide (NO) has been shown to regulate neurotransmitter release in the brain; both inhibitory and excitatory effects
have been seen. Taurine is essential for the development and survival of neural cells and protects them under cell-damaging
conditions. In the brain stem, it regulates many vital functions such as cardiovascular control and arterial blood pressure.
Now we studied the effects of the NO-generating compounds hydroxylamine (HA), S-nitroso-N-acetylpenicillamine (SNAP) and sodium
nitroprusside (SNP) on the release of preloaded [3H]taurine under normal and ischemic conditions in slices prepared from the mouse brain stem from developing (7-day-old) to
young adult (3-month-old) mice. In general, the effects of NO on the release were somewhat complex and difficult to explain,
as expected from the multifunctional role of NO in the central nervous system. The basal initial release under normal conditions
was enhanced by the NO donors 5 mM HA and 1.0 mM SNAP at both ages, but SNP was inhibitory in developing mice. The release
was markedly enhanced by K+ stimulation. The effects of HA, SNAP and SNP on the basal release were not antagonized by the NO synthase inhibitor NG-nitro-L-arginine (L-NNA, 1.0 mM), demonstrating that mechanisms other than NO synthesis are involved. Taurine release in
developing mice in the presence of SNP was reduced by the inhibitor of soluble guanylate cyclase, 1H-(1,2,3)oxadiazolo(4,3-a)quinoxalin-1-one
(ODQ), indicating the possible involvement of cGMP. In normoxia, N-methyl-D-aspartate (NMDA, 1.0 mM) enhanced the SNAP- and
HA-evoked taurine release in developing mice and the HA-evoked release in adults. In ischemia, both K+ stimulation and NMDA potentiated the NO-induced release, particularly in the immature mice, probably without the involvement
of the NO synthase or cGMP. The substantial release of taurine in the developing brain stem evoked by NO donors together with
NMDA might represent signs of important mechanisms against excitotoxicity which protect the brain stem under cell-damaging
conditions.
Authors’ address: Prof. Pirjo Saransaari, Brain Research Center, Medical School University of Tampere, Tampere, FIN-3 3014,
Finland 相似文献
20.
Infiltrating detached maize (Zeamays L.) leaves with L-galactono-1,4-lactone (L-GAL) resulted in a 4-fold increase in the content of leaf ascorbate. Upon exposure to high irradiance (1000 μmol photons m−2 s−1) at 5 °C, L-GAL leaves de-epoxidized the xanthophyll-cycle pigments faster than the control leaves; the maximal ratio of de-epoxidized
xanthophyll-cycle pigments to the whole xanthophyll-cycle pool was the same in both leaf types. The elevated ascorbate content,
together with the faster violaxanthin de-epoxidation, did not affect the degree of photoinhibition and the kinetics of the
recovery from photoinhibition, assayed by monitoring the maximum quantum efficiency of photosystem II primary photochemistry
(Fv/Fm). Under the experimental conditions, the thermal energy dissipation seems to be zeaxanthin-independent since, in contrast
to the de-epoxidation, the decrease in the efficiency of excitation-energy capture by open photosystem II reaction centers (Fv′/Fm′) during the high-irradiance treatment at low temperature showed the same kinetic in both leaf types. This was also observed
for the recovery of the maximal fluorescence after stress. Furthermore, the elevated ascorbate content did not diminish the
degradation of pigments or α-tocopherol when leaves were exposed for up to 24 h to high irradiance at low temperature. Moreover,
a higher content of ascorbate appeared to increase the requirement for reduced glutathione.
Received: 20 May 1999 / Accepted: 29 October 1999 相似文献