滇牡丹内生真菌PR20的鉴定及次生代谢产物的研究

根据形态学特征和18S rDNA序列分析,将滇牡丹根中分离得到的内生真菌菌株PR20鉴定为高大毛壳Chaetomium elatum。利用柱色谱层析方法从该菌株的发酵产物中分离到5个化合物,通过理化性质及波谱数据分析,分别鉴定为7-羟基-4,6二甲基苯酞(1)、苔黑酚(2)、苔色酸(3)、间羟基苯甲酸(4)和次黄嘌呤核苷(5),以上化合物均为首次从滇牡丹内生真菌中分离获得。     

云南红豆杉内生真菌Talarom yces sp.T1BF的化学成分研究

采用色谱技术从云南红豆杉内生真菌Talaromyces sp.T1BF的PDA平板发酵物中分离得到4个化合物.经波谱数据分析及与文献对照,分别鉴定为5α,6α-环氧-24( R)-甲基胆甾-7,22-二烯-3β-醇(1)、醌茜素(2)、苔色酸(3)和间甲基苯酚(4).化合物1为首次从该属真菌中分离获得.     

一株马兜铃内生真菌Colletotrichum sp.代谢产物中细胞毒活性成分的研究

从马兜铃内生真菌Colletotrichum sp.的大米发酵产物中分离得到6个化合物,经波谱数据分析分别鉴定为7-hydroxy-10-oxodehydrodihydrobotrydial(1)、格链孢酚(2)、5-甲氧基格链孢酚(3)、链格孢毒素I(4)、腾毒素(5)和二氢腾毒素(6)。以上化合物均为从该菌属中首次分离得到,其中化合物1～4对肺癌细胞和乳腺癌细胞有一定的细胞毒活性。     

滇牡丹内生真菌Alternaria sp.PR-14的代谢产物研究

从滇牡丹内生真菌交链孢霉属(Alternaria sp.)菌株PR-14的发酵液提取物中分离得到6个化合物,通过波谱技术分别鉴定为交链孢酚(1)、djalonensone(2)、脑苷酯B(3)、脑苷酯C(4)、(2S,3S,4R,2’R)-2-(2’-hydroxytetracosanoylamino)octadecane-1,3,4-triol(5)和甘露糖醇(6),以上化合物均为首次从滇牡丹内生真菌中分离得到。     

小花杜鹃中两个新的木脂素

研究小花杜鹃(Rhododendron minutiflorum)的化学成分及其α-葡萄糖苷酶抑制活性。采用正相硅胶、ODS、Sephadex LH-20和HPLC等多种色谱技术,从小花杜鹃的95%乙醇提取物中分离得到9个化合物,通过波谱分析和文献数据对比,鉴定其结构分别为rhodominutinan A(1)、rhodominutinan B(2)、3,4-di(4-hydroxy-3-methoxybenzyl)tetrahydrofuran(3)、venkatasin(4)、苔色酸甲酯(5)、苔黑酚羧酸乙酯(6)、2,4-二羟基-3,6-二甲基苯甲酸甲酯(7)、芹菜素(8)、山奈酚(9)。其中化合物1和2为新的木脂素,化合物3~9为首次从小花杜鹃中分离得到。通过α-葡萄糖苷酶抑制剂体外筛选模型评价化合物的潜在降血糖活性,结果表明化合物8和9具有较好的α-葡萄糖苷酶抑制活性,IC₅₀值分别为57.51±6.35、54.70±3.67μM。     

刺楸中酚性成分研究

采用常压柱色谱、半制备型高效液相色谱以及制备型薄层色谱等色谱技术结合重结晶等分离纯化手段,从刺楸根中分离得到6个酚性化学成分,通过理化性质和波谱分析鉴定所得化合物分别为苔色酸乙酯(1),2-羟基-4-甲氧基-3,6-二甲基苯甲酸(2),香草醛(3),原儿茶醛(4),原儿茶酸(5)和3-甲氧基苯甲醛(6).所有化合物均为首次从该属植物中获得.     

梅衣的化学成分

从梅衣中分离得到9个化合物,经光谱鉴定,它们分别为黑茶渍素、氯代黑茶渍素、茶渍衣酸、苔黑酚羧酸、苔黑酚羧酸乙酯、β-苔黑酚羧酸甲酯、柔扁枝衣酸、分歧扁枝衣酸及赤星衣酸甲酯,用气相色谱和色谱-质谱联用法,对梅衣精油和梅衣浸膏分别进行了定性、定量分析。梅衣浸膏中共鉴定了37个成分,主要为赤星衣酸乙酯,β-苔黑酚羧酸甲酯、苔黑酚羧酸乙酯等。梅衣精油鉴定了47个成分,未发现有特殊苔香香气的物质。     

罗汉果叶的化学成分研究

为了解罗汉果(Siraitia grosvenorii(Swingle)C.Jeffrey)中的化学成分,利用溶剂萃取和色谱分离手段,从罗汉果叶中分离得到9个化合物。通过光谱分析,分别鉴定为:山奈酚-3,7-O-α-L-二鼠李糖苷(1)、山奈酚-3-O-α-L-鼠李糖苷(2)、阿魏酸(3)、4′-甲氧基二氢槲皮素(4)、大黄素(5)、芦荟大黄素(6)、槲皮素(7)、山奈酚(8)、山奈酚-7-O-α-L-鼠李糖苷(9),其中化合物3~6为首次从罗汉果叶中分离得到。     

广东红冬蛇菰及其内生真菌Penicillium coprophilum Mzz9的化学成分研究

本文研究了广东梅州地区产名贵中药材红冬蛇菰及其内生真菌Penicillium coprophilum Mzz9的化学成分。通过多种色谱技术和波谱学分析共分离鉴定了11个化合物。从红冬蛇菰的二氯甲烷萃取物中分离得到5个化合物:羽扇豆醇(1)、β-香树脂醇(2)、鄁桐甾醇(3)、乙酸蛇麻脂醇酯(4)和亚油酸甘油酯(5);从其内生真菌P.coprophilum Mzz9的发酵液中分离得到6个化合物:去氯灰黄霉素(6)、灰黄霉素(7)、脱氢灰黄霉素(8)、oxaline(9)、4-megastigmen-3,9-dione(10)、对羟基苯乙酮(11)。其中化合物1和5为首次从红冬蛇菰中分离得到,化合物6～11为首次从红冬蛇菰的内生真菌中分离得到。     

嗜低温真菌Pseudogymnoascus pannorum次级代谢产物研究

本文采用现代色谱分离手段从嗜低温真菌Pseudogymnoascus pannorum的大米发酵产物中分离得到8个化合物,采用现代波谱技术鉴定其结构,分别为:L-pyroglutamyl-L-phenylalanine(1)、(R)-N-acetylphenylalanine(2)、(R)-Nacetyltryptophan (3)、methyl 2-hydroxy-4-(3-hydroxy-5-methylphenoxy)-6-methylbenzoate (4)、2-hydroxy-4-(2-hydroxy-3-methoxy-5-methylphenoxyl)-6-methylbenzoate(5)、vanillic acid(6)、1,3,5-三甲氧基苯(7)和亚油酸(8)。其中化合物1和2为首次从天然界分离得到,化合物3～8为首次从假裸囊菌属真菌中分离得到。化合物1～8对人肝癌细胞Hep G-2和人乳腺癌细胞MCF-7未显示细胞毒作用。     

Lipids produced by Epicoccum nigrum in submerged culture

1. The lipids of the red mycelium of the fungus Epicoccum nigrum Link were investigated. Four carotenoid pigments were isolated and identified as beta-carotene, gamma-carotene, rhodoxanthin and torularhodin. 2. For the first time rhodoxanthin was isolated as a fungal metabolite. 3. Linoleic acid was the major fatty acid in the total lipids. 4. Ergosterol was present in the lipids in the non-esterified form.     

虫类中药蛴螬的酚性成分

从虫类中药蛴螬的乙酸乙酯提取物中分离得到6个化合物,其结构经波谱鉴定为3,5-二甲氧基-4-羟基苯甲酸(1),水杨酸(2),对羟基苯甲酸(3),香草酸(4),对羟基苯丙酸(5)和对羟基苯丙酸甲酯(6)。化合物1～6均为首次从该种动物药中分离得到。     

蔓生卷柏的化学成分研究

采用Diaion HP-20、Toyopearl HW-40、Sephadex LH-20和硅胶等多种柱色谱对蔓生卷柏的化学成分进行分离纯化,结合现代波谱技术分离鉴定了5个化合物,分别为邻羟基苯丙酸(1)、邻羟基肉桂酸(2)、5-羟基香豆素(3)、阿曼托双黄酮(4)和(4E,6Z)-2,7-二甲基-8-羟基-辛二烯酸(5)。这些成分均为首次从该植物中分离得到。     

厚叶算盘子的化学成分研究(Ⅱ)

从厚叶算盘子(Glochidion hirsutum)的乙醇提取物中,用硅胶和凝胶柱层析分离得到8个化合物,通过波谱学方法鉴定为bergenin(1),isovitexin(2),isovitexin 7-O-xyloside(3),decoumaroylibotanolide(4),n-butyl-α-D-fructofuranoside(5),n-butyl-β-D-fructofuranoside(6),4-O-ethylgallic acid(7),3-O-methylgallic acid(8)。以上化合物均为首次从该植物中分离得到。     

Purification and immunological characterization of a 12-kDa allergen from Epicoccum purpurascens

Exposure to Epicoccum purpurascens is implicated in respiratory allergies and asthma. Several allergens of clinical importance were identified in Epicoccum extract (EE), but only one allergen has been isolated and characterized. In the present study, a 12-kDa allergen was isolated from an Epicoccum spore-mycelial extract by concanavalin-A sepharose, reverse-phase hydrophobic and gel filtration chromatography. The purified protein was recognized as a single 12-kDa allergen on immunoblot with a serum pool of Epicoccum- sensitive patients. Of the 94 respiratory allergy patients tested intradermally, 17 showed marked positive skin reactions to EE and 12 of them reacted with the 12-kDa protein, indicating a diagnostic sensitivity of 70%. More than 80% patients' sera showed immunoglobulin E (IgE) reactivity to the purified protein in enzyme-linked immunosorbent assay and immunoblot, identifying it as a major allergen. Preincubation of pooled serum with the protein led to inhibition of IgE binding to solid-phase-bound EE (effective concentration 50%=180 ng). Twelve of the 17 serum samples showed significant basophil histamine release upon stimulation with purified protein. The protein induced significant proliferation of peripheral blood mononuclear cells of 13 patients. A high level of interleukin-4 in the culture supernatant of these cells indicated induction of a T-helper type 2 response. The purified 12-kDa protein is a clinically relevant allergen and has potential for the diagnosis and therapy of Epicoccum allergies.     

鞭打绣球中的一个新单萜苷

从鞭打绣球(Hemiphragma heterophyllum Wall.)全草中分离到1个新单萜苷和7个已知化合物.通过波谱数据分析,新单萜苷的结构鉴定为(4S)-α-萜烯醇-8-O-β-D-比喃木糖-(1→6)-β-D-比喃葡萄糖苷,已知化合物分别鉴定为globularin(2)、(2S 3S,4R,9E)-1,3,4-trihydroxy-2-[(2'R)-hydroxy-tetracosanoylamino]-9-octadecene(3)、β-香树素(4)、齐墩果酸(5)、肉桂酸(6)、β-谷甾醇(7)和胡萝卜甙(8).除化合物2外,其余化合物均为首次从鞭打绣球中分离得到.     

Diosgenin glucuronides from Solanum lyratum and their cytotoxicity against tumor cell lines

Bioassay-directed fractionation of the cytotoxicity active fraction of the whole plant from Solanum lyratum led to the isolation of a new steroidal saponin, diosgenin 3-O-beta-D-glucopyranosiduronic acid methyl ester (2), as well as four known compounds, diosgenin (1), diosgenin 3-O-beta-D-glucopyranosiduronic acid (3), diosgenin 3-O-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-glucopyranosiduronic acid (4), diosgenin 3-O-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-glucuroniduronic acid methyl ester (5). The structures of the isolated compounds were elucidated on the basis of their spectral data and chemical evidences. Compound 1 was isolated for the first time from this plant, and compound 3 was isolated as a new natural product. Cytotoxic activities of the isolated compounds were evaluated and the cytotoxicities of compounds 2-5 reported for the first time.     

Fungal endophytes from Acer ginnala Maxim: isolation, identification and their yield of gallic acid

Aims:  The aim of the study was to isolate the endophytic fungi from Acer ginnala and screen isolates rich in gallic acid.
Methods and Results:  After epiphytic sterilization, 145 fungal endophytes were isolated from the stem, annual twig and seed of Acer ginnala . The endophytes were grouped into ten different taxa, Phomopsis sp., Neurospora sp., Phoma sp., Epicoccum sp., Penicillium sp., Alternaria sp., Fusarium sp., Trichoderma sp., Cladosporium sp. and a species of Pleosporales Incertae Sedis , by their morphological traits and ITS-rDNA sequence analysis. The content and yield of gallic acid of 141 isolates were determined by HPLC. On average, the species of Pleosporales Incertae Sedis had the highest content and yield of gallic acid (13·28 mg g⁻¹ DW; 119·62 mg l⁻¹), while Alternaria sp. had the lowest.
Conclusions:  Of 141 fungal endophytes from A. ginnala , Phomopsis sp. isolate SX10 showed both the highest content and the highest yield of gallic acid (29·25 mg g⁻¹ DW; 200·47 mg l⁻¹).
Significance and Impact of the Study:  Endophytic fungi isolated from A. ginnala may be used as potential producers of gallic acid and other compounds with biological activities, or functioned as elicitors to produce natural compounds.     

藏药翁布的酚性成分研究

从藏药翁布(Myricaria germanica)的60％丙酮提取物中进行了进一步研究,从中共分离得到了11个化合物.利用光谱和波谱分析法,分别鉴定为阿魏酸(1),松柏醇(2),阿魏酸葡糖苷(3),异落叶松脂醇(4),咖啡酸(5),对羟基桂皮酸(6),没食子酸(7),3,5-二羟基-4-甲氧基苯甲酸(8),杜鹃醇(9),3-甲氧基-4-羟基-苯甲酸(10)和3,4,5-三羟基肉桂酸(11).化合物1～11均为首次从该植物中分得,其中1～6和8～11为首次从水柏枝属植物中分离得到.     

木棉叶酚性成分研究

采用硅胶、MCI和Sephadex LH-20层析方法对木棉叶的化学成分进行分离纯化,鉴定了12个酚性化合物,分别为芒果苷(1)、东莨菪内酯(2)、滨蒿内酯(3)、原儿茶酸(4)、七叶内酯(5)、柠檬油素(6)、龙胆酸(7)、东莨菪苷(8)、七叶苷(9)、丁香酸葡萄糖苷(10)、槲皮素(11)和木犀草素-4’-葡萄糖苷(12)。除化合物1和11,其余化合物均为首次从该属植物中分得。