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Cell cycle-regulated gene expression in transgenic plant cells 总被引:2,自引:0,他引:2
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Cooperation of two distinct cis-acting elements is necessary for the S phase-specific activation of the wheat histone H3 promoter 总被引:2,自引:2,他引:0
Norihiro Ohtsubo Takuya Nakayama Hidetaka Kaya Rie Terada Ko Shimamoto Tetsuo Meshi Masaki Iwabuchi 《The Plant journal : for cell and molecular biology》1997,11(6):1219-1225
We have previously shown that the proximal promoter region (−185 to +57) of the wheat histone H3 gene ( TH012 ) is sufficient for regulating S phase-specific expression of a reporter GUS gene. To define the cis -acting element(s) responsible for S phase-specific expression, GUS fusion genes under the control of wild-type or variously mutated H3 promoters were stably introduced into cultured rice Oc cells and their temporal expression was analyzed during the cell cycle by quantitative S1 analysis. The S phase-specific expression of the full-sized promoter (−1716 to +52) was significantly impaired by short internal deletions disrupting the type I element from −175 to −158 (CCACGTCACCaATCCGCG), composed of the Hex (CCACG-TCA) and reverse-oriented Oct (GATCCGCG) motifs. Moreover, the H3 proximal promoters (−184 to +52) harboring base-substitution mutations in either or both of the Hex and Oct motifs could no longer activate gene expression during the S phase. These results indicate that the type I element is the first cis-acting element identified responsible for the S phase-specific expression of plant histone genes. Results also suggested the presence of a redundant cis -acting element(s) responsible for S phase-specific expression in the H3 far-upstream region (−1716 to −185). 相似文献
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