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1.
Summary Using fluorescent probes and confocal laser scanning microscopy we have examined the organisation of the microtubule and actin components of the cytoskeleton in kidney-shaped guard cells of six species of Selaginella. The stomata of Selaginella exhibit novel cytoskeletal arrangements, and at different developmental stages, display similarities in microtubule organisation to the two major types of stomata: grass (dumbbell-shaped) and non-grass (kidney-shaped). Initially, cortical microtubules and F-actin radiate from the stomatal pore and extend across the external and internal periclinal cell surfaces of the guard cells. As the stomata differentiate, the cytoskeleton reorients only along the internal periclinal walls. Reorganisation is synchronous in guard cells of the same stoma. Microtubules on the inner periclinal walls of the guard cells now emanate from areas of the ventral wall on either side of the pore and form concentric circles around the pore. The rearrangement of F-actin is similar to that of microtubules although F-actin is less well organised. Radial arrays of both microtubules and F-actin are maintained adjacent to the external surfaces. Subsequently, in two of the six species of Selaginella examined, microtubules on both the internal and external walls become oriented longitudinally and exhibit no association with the ventral wall. In the other four species, microtubules adjacent to the internal walls revert to the initial radial alignment. These findings may have implications in the development and evolution of the stomatal complex.Abbreviations GC
guard cell
- MT
microtubule 相似文献
2.
Stomatal regulation is essential for the growth of land plants. Pairs of guard cells that delineate the stomata perceive stimuli
and respond to acquire the optimum aperture. The actin cytoskeleton participates in signaling pathways of the guard cell (Kim
et al., 1995; Eun and Lee, 1997; Hwang et al., 1997). To identify the upstream molecules that regulate actin dynamics in plant
cells, we immunoblotted proteins extracted from leaves ofCommelina commuais L. with the RhoA antibody, and identified one band of 26KD from the epidermis. Using immunofluorescence microscopy, we examined
the subcellular distribution of the immuno-reactant(s) in guard cells. When stomata were open under light, the organization
of the immuno-reactant(s) resembled the radial arrangement of cortical actin filaments of guard cells. Double-labeling of
the guard cells, using the RhoA and actin antibodies as primary antibodies, showed that the immuno-reactant(s) of the RhoA
antibody and actin filaments co-localized in the cortex of illuminated guard cells. However, the pattern was not found in
guard cells when stomata were closed under darkness or by ABA, conditions under which cortical actin proteins are disassembled
in guard cells. From these observations, we can suggest the possible presence of a RhoA-like protein and its involvement in
the organization of the actin cytoskeleton in guard cells. 相似文献
3.
XIN-QI GAO XIU-LING WANG FEI REN JIA CHEN & XUE-CHEN WANG 《Plant, cell & environment》2009,32(8):1108-1116
Vacuoles and actin filaments are important cytoarchitectures involved in guard cell function. The changes in the morphology and number of vacuoles and the regulation of ion channel activity in tonoplast of guard cells are essential for stomatal movement. A number of studies have investigated the regulation of ion channels in animal and plant cells; however, little is known about the regulating mechanism for vacuolar dynamics in stomatal movement. Actin filaments of guard cells are remodelling with the changes in the stomatal aperture; however, the dynamic functions of actin filaments in stomatal movement remain elusive. In this paper, we summarize the recent developments in the understanding of the dynamics of actin filaments and vacuoles of guard cells during stomatal movement. All relevant studies suggest that actin filaments might be involved in stomatal movement by regulating vacuolar dynamics and the ion channels in tonoplast. The future study could be focused on the linker protein mediating the interaction between actin filaments and tonoplast, which will provide insights into the interactive function of actin and vacuole in stomatal movement regulation. 相似文献
4.
Stomatal opening by fusicoccin is accompanied by depolymerization of actin filaments in guard cells 总被引:6,自引:0,他引:6
Actin in guard cells is assembled in a radial pattern when stomata are induced to open under light, but the filaments are
disassembled when stomata are closed under darkness or by abscisic acid (S.-O. Eun and Y. Lee, 1997, Plant Physiol. 115: 1491–1498).
To test if signals that open stomata commonly generate the polymerized form of actin in guard cells, leaves of Commelina communis L. were treated with a potent stomatal opening agent, fusicoccin, and the actin organization examined by immunolocalization
techniques. When stomata were induced to open by fusicoccin, hardly any of the filamentous form of actin was detected; instead,
the actin resembled that present in guard cells that had been treated with an antagonist to actin filaments, cytochalasin
D, and showed a sharp contrast to the long filaments developed in illuminated guard cells. Furthermore, treatment of illuminated
leaves with fusicoccin disintegrated actin filaments that had already been formed in the guard cells. Preincubation of leaves
with phalloidin, which interferes with fusicoccin-induced actin depolymerization, delayed fusicoccin-induced opening during
the early phase. These observations suggest that the prevention of actin filament formation and/or depolymerization of actin
filaments may accelerate the stomatal opening process in response to fusicoccin.
Received: 1 October 1999 / Accepted: 29 November 1999 相似文献
5.
Cortical actin filaments in guard cells respond differently to abscisic acid in wild-type and abi1-1 mutant Arabidopsis 总被引:1,自引:0,他引:1
Cortical actin filaments in guard cells of Commelina communis L. show signal-specific organization during stomatal movements [S.-O. Eun and Y. Lee (1997) Plant Physiol 115: 1491–1498;
S.-O. Eun and Y. Lee (2000) Planta 210: 1014–1017]. To study the roles of actin in signal transduction, it is advantageous
to use Arabidopsis thaliana (L.) Heynh., an excellent model plant with numerous well-characterized mutants. Using an immunolocalization technique, we
found that actin deployments in guard cells of A. thaliana were basically identical to those in C. communis: actin proteins were assembled into radial filaments under illumination, and were disassembled by ABA. In addition, we examined
actin organization in an ABA-insensitive mutant (abi1-1) to test the involvement of protein phosphatase 2C (PP2C) in the control of actin structure. A clear difference was observed
after ABA treatment, namely, neither stomatal closing nor depolymerization of actin filaments was observed in guard cells
of the mutant. Our results indicate that PP2C participates in ABA-induced actin changes in guard cells.
Received: 23 June 2000 / Accepted: 20 October 2000 相似文献
6.
Actin filaments and chloroplasts in guard cells play roles in stomatal function. However, detailed actin dynamics vary, and the roles that they play in chloroplast localization during stomatal movement remain to be determined. We examined the dynamics of actin filaments and chloroplast localization in transgenic tobacco expressing green fluorescent protein (GFP)-mouse talin in guard cells by time-lapse imaging. Actin filaments showed sliding, bundling and branching dynamics in moving guard cells. During stomatal movement, long filaments can be severed into small fragments, which can form longer filaments by end-joining activities. With chloroplast movement, actin filaments near chloroplasts showed severing and elongation activity in guard cells during stomatal movement. Cytochalasin B treatment abolished elongation, bundling and branching activities of actin filaments in guard cells, and these changes of actin filaments, and as a result, more chloroplasts were localized at the centre of guard cells. However, chloroplast turning to avoid high light, and sliding of actin fragments near the chloroplast, was unaffected following cytochalasin B treatment in guard cells. We suggest that the sliding dynamics of actin may play roles in chloroplast turning in guard cells. Our results indicate that the stochastic dynamics of actin filaments in guard cells regulate chloroplast localization during stomatal movement. 相似文献
7.
Summary The ultrastructural study of cross sections of normal skeletal muscle cells showed the existence of irregular patterns of actin filaments in connection with the hexagonal pattern of the myosin filaments. The actin filaments surrounding each myosin filament vary in number from 6 to 11. The most frequent relationship is 9 to 1, followed by 10 to 1 and 8 to 1. The hexagonal pattern of actin filaments was observed only in the 6 to 1 arrays; as the actin filaments increase in number, they tend to form different polygons or circles around the myosin filaments. All described patterns may occur in each sarcomere. The actin to myosin filament ratio varies from 3 to 4 within each individual myofibril. The described variability of the actin filaments arrays leads to several difficulties in an explanation of the mechanism of muscular contraction.Director, Chief of Section, Histology. Profesor Agregado de Embriología e HistologíaProfesor Adjunto de Embriología e HistologíaResidente de Anatomía Patol'ogica de la Ciudad Sanitaria La Paz 相似文献
8.
Our three-dimensional (3-D) images showed that paxillin co-localized on actin filaments as fibrous structures, as well as clusters, in endothelial cells (ECs). In living ECs under flow condition, we monitored concurrently the intracellular dynamics of DsRed2-paxillin and GFP-actin by time-lapse video recording and dual-color fluorescence imaging. The results showed that the dynamic motion of paxillin as fibrous structures was associated with actin filaments, but not with microtubules. Our findings suggest that the actin network plays an important role not only in the assembly/disassembly of paxillin at focal adhesions, but also as a track for the intracellular transport of paxillin, which is involved in signaling pathway. 相似文献
9.
Microtubule and actin filament organization during stomatal morphogenesis in the fernAsplenium nidus
Summary The newly-formed guard cell mother cells (GMCs) ofAsplenium nidus are small, lens-shaped and are formed by one or two asymmetrical divisions. Their growth axis is parallel to the plane of their future division, a process during which the internal periclinal wall (IPW) is detached from the partner wall of the underlying cell(s). This oriented GMC expansion occurs transversely to a microfibril bundle, which is deposited externally to a U-like microtubule (Mt) bundle and a co-localized actin filament (Af) bundle. They line the IPW and the major part of the anticlinal walls. The deposition of the microfibril bundle is followed by the slight constriction of the internal part of the GMCs and the broadening of the substomatal cavity. The IPW forms a distinct bulging distal to the neighbouring leaf margin, as well as a less defined proximal one. During the IPW bulging, the Mts and Afs under the external periclinal wall (EPW) attain a radial organization. This is followed by thinning of the central EPW region, which becomes impregnated with a callose-like glucan. The rest of the EPW becomes unequally thickened. The disintegration of the U-like Mt bundle is succeeded by the organization of radial Mt and Af arrays under the IPW. The radial Mt systems, controlling the alignment of the newly-deposited microfibrils, allow the GMC to assume a round paradermal profile. The GMCs form a preprophase Mt band (PPB) perpendicular to the interphase U-like Mt bundle. The anticlinal PPB portions appear first and those lining the periclinal walls later. The cytoplasm adjacent to the latter walls retain the radial Mt systems during early preprophase, simultaneously with the anticlinal PPB portions. The observations suggest that the GMCs of the fernA. nidus obtain a unique form, as a result of a particular polarity established in the cortical cytoplasm of the periclinal walls, in which Mts and Afs appear involved. This polarity persists in cell division and is inherited to guard cells (GCs). It provides primary morphogenetic information not only to GMCs but also to GCs.Abbreviations Af
actin filament
- EPW
external periclinal wall
- GC
guard cell
- GMC
guard cell mother cell
- IPW
internal periclinal wall
- Mt
microtubule
- MTOC
microtubule organizing centre
- PPB
preprophase microtubule band 相似文献
10.
Summary Muscle actin filaments labeled with rhodamine-phalloidin were observed to move on the surface coated with a crude extract of pollen tubes ofLilium longiflorum with an average velocity of 1.99±0.55 m/sec. The movement required both Mg2+ and ATP. These results indicate that the extract of pollen tubes contains a myosin-like translocatorAbbreviations ATP
adenosine-5-triphosphate
- DTT
dithiothreitol
- EGTA
ethyleneglycol-bis-(-aminoethylether)N,N,N,N-tetraacetic acid
- PIPES
piperazine-N,N-bis-(2-ethanesulfonic acid)
- PMSF
phenylmethylsulfonyl fluoride 相似文献
11.
Postnatal change in the distribution of actin filaments in endothelial cells was studied in the rat aorta by use of rhodamine-phalloidin staining and confocal laser scanning microscopy. Endothelial cells of the rat aorta possessed two populations of actin filament bundles, namely, peripheral bands at the cell border and stress fibers running longitudinally in the cytoplasm. Aortic endothelial cells of the neonatal rat contained only stress fibers, whereas those of the 10-day-old rat developed both peripheral bands and stress fibers. After 20 days of age, aortic endothelial cells had predominantly peripheral bands with occasional stress fibers around the branch orifices. During postnatal development the length density of stress fibers in aortic endothelial cells decreased, whereas individual stress fibers in endothelial cells were shortened. Electron-microscopic observation revealed that the high intercellular boundaries of aortic endothelial cells at birth decreased in height and developed cytoplasmic interdigitations after 20 days of age. The occurrence of peripheral bands at the cell border is thought to be closely related to formation of cytoplasmic interdigitation which strengthens the mechanical connection between endothelial cells against increasing transmural pressure. Expression of stress fibers in aortic endothelial cells of the neonatal rat is supposed to be affected by longitudinal elongation of the developing aorta, whereas their postnatal decrease is though to be correlated with the change of fluid shear stress loaded in the aortic endothelium. 相似文献
12.
The reorganization of actin filaments (AFs) and vacuoles in guard cells is involved in the regulation of stomatal movement. However, it remains unclear whether there is any interaction between the reorganization of AFs and vacuolar changes during stomatal movement. Here, we report the relationship between the reorganization of AFs and vacuolar fusion revealed in pharmacological experiments, and characterizing stomatal opening in actin‐related protein 2 (arp2) and arp3 mutants. Our results show that cytochalasin‐D‐induced depolymerization or phalloidin‐induced stabilization of AFs leads to an increase in small unfused vacuoles during stomatal opening in wild‐type (WT) Arabidopsis plants. Light‐induced stomatal opening is retarded and vacuolar fusion in guard cells is impaired in the mutants, in which the reorganization and the dynamic parameters of AFs are aberrant compared with those of the WT. In WT, AFs tightly surround the small separated vacuoles, forming a ring that encircles the boundary membranes of vacuoles partly fused during stomatal opening. In contrast, in the mutants, most AFs and actin patches accumulate abnormally around the nuclei of the guard cells, which probably further impair vacuolar fusion and retard stomatal opening. Our results suggest that the reorganization of AFs regulates vacuolar fusion in guard cells during stomatal opening. 相似文献
13.
The energy required for ion uptake in guard cells is provided by two important bioenergetic processes, namely respiration and photosynthesis. The blue light-sensitive plasma membrane redox system is considered as the third bioenergetic phenomenon, since it uses blue light to create a proton gradient across the membrane. The unique features of respiration and photosynthesis in guard cells and their role in stomatal function are emphasized. Evidence for and against the blue light-sensitive components on plasma membrane (ATPase/distinct redox chain) and the photoreceptors (flavins, carotenoids, pterins) in guard cells are presented. The information on ion channels and their response to various kinds of secondary messengers including G-proteins, phosphoinositides, diacylglycerol, calcium, cAMP and protein kinases are reviewed. A model is presented indicating the possible mechanism of perception and transduction by guard cells of external signals and their interaction with different bioenergetic components. 相似文献
14.
Summary Mouse eggs at fertilization were permeated with glycerol solutions and then reacted with heavy meromyosin to show actin filaments by electron microscopy. The meiotic area of the egg surface is devoid of microvilli and is supported by a thick layer (0.6–0.8 m in width) of submembranous filaments. A much thinner layer (less than 0.3 m) is present in the remaining non-meiotic microvillous area and underlying its membrane is a very thick layer of cross-filaments and filament bundles. 相似文献
15.
Structural interactions of actin filaments and endoplasmic reticulum in honeybee photoreceptor cells
Otto Baumann 《Cell and tissue research》1992,268(1):71-79
Summary Fluorescent phallotoxins and heavy meromyosin were used to reveal the organization of the actin cytoskeleton in honeybee photoreceptor cells, and the relationship of actin filaments to the submicrovillar, palisade-like cisternae of the endoplasmic reticulum (ER). Bundles of unipolar actin filaments (pointed end towards the cell center) protrude from the microvillar bases and extend through cytoplasmic bridges that traverse the submicrovillar ER. Within the cytoplasmic bridges, the filaments are regularly spaced and tightly apposed to the ER membrane. In addition, actin filaments are deployed close to the microvillar bases to form a loose web. Actin filaments are scarce in cell areas remote from the rhabdom; these areas contain microtubule-associated ER domains. The results suggest that the actin system of the submicrovillar cytoplasm shapes the submicrovillar ER cisternae, and that the distinct ER domains interact with different cytoskeletal elements. 相似文献
16.
Association of mitochondria with intermediate filaments and of polyribosomes with cytoplasmic actin 总被引:5,自引:0,他引:5
Summary Anti-mitochondrial autoantibody and fluorescent derivatives of insulin stain phase-dense mitochondria in acetone-fixed monolayers of fibroblasts. Double fluorochrome studies show mitochondria in close topographic association with intermediate filaments. In cells treated with vinblastine or colchicine, mitochondria are relocated in sites closely associated with coils of perinuclear intermediate filaments. In contrast, autoantibody to polyribosomes stains granules aligned in the long axis of well spread embryonic cells, in the direction of actin-containing fibrils, an arrangement that is lost in cells pretreated with the actin filament disrupting drug cytochalasin B. In more mature fibroblasts, antiribosomal antibody reacts with phase-dense rough endoplasmic reticulum and this staining pattern is not affected by cytochalasin B. The observations suggest that mitochondria are associated with intermediate filaments and that free polyribosomes, but not polyribosomes attached to rough endoplasmic reticulum, are associated with cytoplasmic actin.Supported by a grant from the Anti-Cancer Council of Victoria. We thank Mrs. I. Burns for technical assistance and Dr. H.A. Ward and staff for preparation of fluorescent conjugates 相似文献
17.
Excellent visualisation of microtubules and actin filaments was obtained in fixed tobacco BY-2 suspension cells after optimising a protocol for whole mount immunolabelling. The procedure is based on modification of fixation, cell wall digestion, dimethyl sulfoxide (DMSO) treatment, post fixation, and blocking. The most critical aspects of successful preservation and visualization of cytoskeletal elements appeared to be: a two-step fixation with paraformaldehyde and glutaraldehyde before enzymatic cell wall digestion and a post fixation with aldehydes thereafter. The method allows the improved visualization of the organisation of the microtubular and actin filament arrays during the successive stages of cell division and at interphase. Although we present the application of our protocols for cytoskeleton labelling, the excellent results show the potential of using this method for the analysis of various proteins and molecules in plant cells.Electronic Supplementary Material Supplementary material is available for this article at 相似文献
18.
Summary The ultrastructural organization of myofilaments in skeletal muscle was studied in four mammalian species (mouse, rat, hamster, goat). In all these species, myofibrils showing irregularly distributed arrays of a variable number of actin filaments (from 6 to 11) were observed. The proportion of such myofibrils and the predominant patterns of actin filaments varied from one species to another. These results are in agreement with those previously reported for human skeletal muscle. 相似文献
19.
SHU‐WEI WANG YING LI XIAO‐LU ZHANG HAI‐QIANG YANG XUE‐FEI HAN ZHAO‐HUI LIU ZHONG‐LIN SHANG TOMOYA ASANO YASUSHI YOSHIOKA CHUN‐GUANG ZHANG YU‐LING CHEN 《Plant, cell & environment》2014,37(9):2201-2210
Controversies regarding the function of guard cell chloroplasts and the contribution of mesophyll in stomatal movements have persisted for several decades. Here, by comparing the stomatal opening of guard cells with (crl‐ch) or without chloroplasts (crl‐no ch) in one epidermis of crl (crumpled leaf) mutant in Arabidopsis, we showed that stomatal apertures of crl‐no ch were approximately 65–70% those of crl‐ch and approximately 50–60% those of wild type. The weakened stomatal opening in crl‐no ch could be partially restored by imposing lower extracellular pH. Correspondingly, the external pH changes and K+ accumulations following fusicoccin (FC) treatment were greatly reduced in the guard cells of crl‐no ch compared with crl‐ch and wild type. Determination of the relative ATP levels in individual cells showed that crl‐no ch guard cells contained considerably lower levels of ATP than did crl‐ch and wild type after 2 h of white light illumination. In addition, guard cell ATP levels were lower in the epidermis than in leaves, which is consistent with the observed weaker stomatal opening response to white light in the epidermis than in leaves. These results provide evidence that both guard cell chloroplasts and mesophyll contribute to the ATP source for H+ extrusion by guard cells. 相似文献
20.
Summary The ultrastructural organization of actin filaments was studied in the neurohypophysial system of the rat after heavy meromyosin (HMM) labeling. This structural pattern is characterized by (1) a straight arrangement of the filaments parallel to the axonal axis in the proximal nondilated parts of axons, (2) a central location within axonal dilatations, and (3) a higher concentration within axonal endings where the filaments form a complex three-dimensional network. The relationships of the filaments to other axonal structures and organelles was further studied by use of electron microscopic stereoscopy. The actin filaments frequently appear anchored to the axolemma with either polar arrangements of the arrowhead decoration (i) at structurally undifferentiated sites, and (ii) more particularly within perivascular endings, at sites with electron-dense thickenings. In all axonal divisions actin filaments are also found to bind to filamentous material surrounding the microtubules and to various organelles. Within the terminal portions of the axons actin filaments exhibit close relationships to neurosecretory granules and to the numerous smooth microvesicles found in this region. Such preferential relationships are particularly observed both in axon terminals and in pituicytes, with coated vesicles frequently binding actin filaments. In water-deprived rats, the concentration of actin filaments is conspicuously increased along the axons and more clearly in the axonal swellings and endings, where they form a more complex and interconnected network. These data are discussed in the light of a possible involvement of contractile proteins in the mechanisms of axonal transport and terminal release of neurosecretory products. 相似文献