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Towards royal jelly proteome 总被引:4,自引:0,他引:4
Scarselli R Donadio E Giuffrida MG Fortunato D Conti A Balestreri E Felicioli R Pinzauti M Sabatini AG Felicioli A 《Proteomics》2005,5(3):769-776
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《Journal of Asia》2020,23(2):445-448
Major royal jelly proteins (MRJPs) of honeybee royal jelly (RJ) exhibit antimicrobial and antioxidant activities. Although MRJPs of Apis mellifera RJ (AmMRJPs) responsible for antibacterial activity have been identified, AmMRJPs with antioxidant effects remain to be elucidated. Here we identified and compared the antioxidant activities of purified recombinant AmMRJPs 1–7, which are expressed in baculovirus-infected insect cells. Antioxidant assays of recombinant AmMRJPs 1–7 against H2O2 revealed that AmMRJPs reduce caspase-3 activity and oxidative stress-induced cell apoptosis and lead to increased cell viability. Consistent with these results, AmMRJPs 1–7 exhibit 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity and protect against oxidative DNA damage. These results indicate that AmMRJPs play a role as antioxidants in A. mellifera RJ. 相似文献
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The chemical nature of royal jelly 总被引:3,自引:0,他引:3
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《Journal of Asia》2019,22(3):737-741
Major royal jelly proteins (MRJPs) are the protein components in royal jelly (RJ). MRJPs 1–7 are detected in the honeybee Apis mellifera RJ. Although A. mellifera MRJP (AmMRJP) 2 exhibited antibacterial activity, the other MRJPs with antimicrobial activities in A. mellifera RJ remains largely unknown. Here, we compared the antibacterial activity of recombinant AmMRJPs 1–7 expressed in baculovirus-infected insect cells. Antibacterial assays of recombinant AmMRJPs 1–7 against the gram-negative bacterium Escherichia coli revealed that AmMRJPs 2–5 and 7 exhibited antibacterial activity, whereas AmMRJPs 1 and 6 displayed almost no antibacterial activity. Consistent with the antibacterial activity of AmMRJPs, AmMRJPs 2–5 and 7 are bound to bacterial cell walls. These results indicated that AmMRJPs 2–5 and 7 contribute directly to the antibacterial property of RJ, suggesting that MRJPs play a role in the antimicrobial property of RJ. 相似文献
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《Journal of Asia》2019,22(3):684-689
Royal jelly (RJ) is a well-known functional and medicinal food for human health promotion. Major royal jelly proteins (MRJPs), which are the major protein components in RJ, exhibit antimicrobial activities. However, the identities of the MRJPs of RJ responsible for its antioxidant effects have remained unclear. Here, we report that honeybee (Apis cerana) MRJP 2 (AcMRJP2) acts as an antimicrobial and antioxidant agent in RJ. Using recombinant AcMRJP2, which was produced in baculovirus-infected insect cells, we established the antimicrobial and antioxidant roles of MRJP 2. AcMRJP2 bound to the surfaces of bacteria, fungi, and yeast, which then induced structural damage in the microbial cell walls and led to a broad spectrum of antimicrobial activities. AcMRJP2 protected mammalian and insect cells via the direct shielding of the cell against oxidative stress, which led to reduced levels of caspase-3 activity and oxidative stress-induced cell apoptosis, followed by increased cell viability. Moreover, AcMRJP2 exhibited DNA protection activity against reactive oxygen species (ROS). Our data indicate that AcMRJP2 could play a crucial role as an antimicrobial agent and antioxidant in RJ, suggesting that MRJP 2 is a component responsible for the antimicrobial and antioxidant activities of RJ. 相似文献
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蜂王浆蛋白生物学功能的研究 总被引:4,自引:0,他引:4
蜂王浆是哺育蜂咽下腺与上颚腺分泌的供3日龄以内蜜蜂幼虫和蜂王食用的浆状物质,具有多种生物活性。王浆含有丰富的蛋白质,通过直接分离纯化王浆中的蛋白质分子,或通过王浆蛋白基因克隆表达以获得表达产物,进而研究王浆蛋白的功能。研究发现王浆中含有多种具有特定功能的蛋白质,如MRJP3具有免疫调节作用,Jelleine-I-IV及Royalisin具有抗菌作用,MRJP1具有抗肿瘤功能及可能的蜜蜂行为调节功能,57kDa蛋白及Apisimin的促细胞生长功能,57kDa蛋白具有抗疲劳功能等。随着王浆蛋白组分分离、基因克隆表达及其功能研究的深入,王浆蛋白将被应用到生物医药、细胞培养、组织工程等更多的研究领域。 相似文献
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Towards posttranslational modification proteome of royal jelly 总被引:1,自引:0,他引:1
Royal jelly (RJ) is a secretory protein from the hypopharyngeal glands of nurse honeybee workers, which contains a variety of proteins of which major royal jelly proteins (MRJPs) are some of the most important. It plays important roles both for honeybee and human. Each family of MRJP 1-5 displays a string of modified protein spots in the RJ proteome profile, which may be caused by posttranslational modifications (PTMs) of MRJPs. However, information on the RJ PTMs is still limited. Therefore, the PTM status of RJ was identified by using complementary proteome strategies of two-dimensional gel electrophoresis (2-DE), shotgun analysis in combination with high performance liquid chromatography-chip/electrospray ionization quadrupole time-of-flight/tandem mass spectrometry and bioinformatics. Phosphorylation was characterized in MRJP 1, MRJP 2 and apolipophorin-III-like protein for the first time and a new site was localized in venom protein 2 precursor. Methylation and deamidation were also identified in most of the MRJPs. The results indicate that methylation is the most important PTM of MRJPs that triggers the polymorphism of MRJP 1-5 in the RJ proteome. Our data provide a comprehensive catalog of several important PTMs in RJ and add valuable information towards assessing both the biological roles of these PTMs and deciphering the mechanisms underlying the beneficial effects of RJ for human health. 相似文献
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Maria Dimou Chrysoula Tananaki Georgios Goras Emmanuel Karazafiris Andreas Thrasyvoulou 《Grana》2013,52(2):106-112
Microscopic analysis of 82 royal jelly samples obtained from several locations in Greece was carried out in order to study the pollen spectrum of Greek royal jelly. The analysis recorded over 60 taxa. The abundance of each pollen type varied among the areas and the samples. The most frequent pollen types found in the samples were Brassicaceae, Olea europaea, Eucalyptus, Trifolium, Rubus, Carduus-type, Hypericum, Daucus-type and Cistus. The majority of the pollen types found in the royal jelly samples were also found at the pollen flora around the apiaries. 相似文献
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作为社会性昆虫,蜜蜂是研究社会行为和学习记忆的理想模式生物。王浆主蛋白(Major royal jelly protein, MRJP)是蜂王浆中蛋白质的主要成分,该家族一共有9个成员,MRJP1~MRJP9。所有mrjps均以串联排列的形式位于蜜蜂11号染色体上一个大约60 kb的DNA片段上。mrjp的同源体也存在于其他的膜翅目昆虫,均是通过yellow进化而来的。随着不断地进化,MRJPs家族进化出许多重要功能,其中最主要的就是营养功能。本文从MRJPs家族的基因及蛋白质结构、mRNA表达情况、进化和功能等方面进行综述,为今后开展相关研究提供理论支持。 相似文献
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Jinhui Zhou Jing Zhao Xiaofeng Xue Jinzhen Zhang Fang Chen Yi Li Liming Wu Chenchen Li Jianqiu Mi 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2009,877(32):4164-4170
Melamine has become the focus of attention for the possible occurrence of nephrolithiasis and associated deaths, because it was added to foods to increase the apparent protein content by unethical manufacturers. An analytical method based on hydrophilic interaction chromatography/tandem mass spectrometry (HILIC-MS/MS) was developed and validated for the determination of melamine in the royal jelly (RJ) and royal jelly lyophilized powder (RJLP). Trace of melamine was extracted from the RJ and RJLP by ultrasonic-assisted extraction followed by clean-up procedure using mixed-mode cation exchange (MCX) solid phase extraction and separated on a hydrophilic interaction chromatography (HILIC) analytical column with acetonitrile/5 mM ammonium acetate buffer (88:12, v/v) as mobile phase. Detection was carried out by positive electrospray ionization (ESI+) in multiple reaction monitoring (MRM) mode. The chromatographic separation was obtained within 5 min and was linear in the concentration range of 0.01–8 μg/mL in RJ and 0.05–10 μg/mL in RJLP for melamine. The mean extraction recoveries for melamine were ranged from 89.6 to 100.4%. Method validation parameters were evaluated such as linearity, selectivity, precision, carryover and recovery, giving results within the acceptable range. The proposed method was successfully applied to the quantitation of melamine in RJ and RJLP. This approach will be of particular utility for the evaluation of melamine residue level and routine monitor of melamine in RJ and RJLP samples. 相似文献
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Karl J. Kramer Howard S. Tager Cynthia N. Childs Roy D. Speirs 《Journal of insect physiology》1977,23(2):293-295
An aqueous extract of royal jelly from Apis mellifera produced hypoglycemia when injected into larvae of Manduca sexta. Application of specific radioimmunoassay to the partially-purified extract showed that royal jelly contains several insulin-like peptides, the major immunoreactive component of which has an apparent mol. wt similar to that of bovine insulin. These results suggest the existence of a peptide in the honeybee having both biological and structural similarities to vertebrate insulin. 相似文献
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《Insect Biochemistry》1982,12(1):91-98
Insulin-like peptides from the haemolymph of the tobacco hornworm, Manduca sexta L. and from royal jelly of the honey bee, Apis mellifera L., were purified by acid extraction, ion-exchange chromatography, gel filtration and affinity chromatography. Quantities detected were 500 and 25 pg porcine insulin equivalents per gram of lyophilized material for haemolymph and royal jelly, respectively. M. sexta insulin was similar to vertebrate insulin in solubility, chromatographic, immunological and biological properties. Amino acid compositions were comparable except for serine, leucine and phenylalanine. The A. mellifera peptide had similar properties, but was not isolated in large enough quantity to determine its amino acid composition. These results demonstrated that insect insulin and vertebrate insulin are structurally similar. 相似文献
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Andreas Stocker Peter Schramel Antonius Kettrup Eberhard Bengsch 《Journal of trace elements in medicine and biology》2005,19(2-3):183-189
Royal jelly from Apis mellifera is a highly active natural biological substance and is probably one of the most interesting raw substances in natural product chemistry. Trace elements play a key role in the biomedical activities associated with royal jelly, as these elements have a multitude of known and unknown biological functions. For this reason concentrations of 28 trace (Al, Ba, Sr, Bi, Cd, Hg, Pb, Sn, Te, Tl, W, Sb, Cr, Ni, Ti, V, Co, Mo) and mineral (P, S, Ca, Mg, K, Na, Zn, Fe, Cu, Mn) elements were systematically investigated in botanically and geologically defined royal jelly samples. In addition, concentrations of 14 trace elements were measured in the associated honey samples--honey being the precursor of royal jelly. Concentrations of K, Na, Mg, Ca, P, S, Cu, Fe, Zn, Al, Ba and Sr in royal jelly were determined by inductively coupled plasma optical emission spectroscopy (ICP-OES), while concentrations of Bi, Cd, Hg, Pb, Sn, Te, Tl, W, Sb, Cr, Mn, Ni, Ti, V, Co and Mo in royal jelly were determined by double focusing magnetic sector field inductively coupled plasma mass spectrometry (SF-ICP-MS). In the honey samples, trace and mineral element concentrations strongly depended on botanical and geological origin, and substantial variation was found. In contrast, the concentrations of trace and mineral elements were highly constant in the associated royal jelly samples. The most important results were the homeostatic adjustments of trace and mineral element concentrations in royal jelly. This effect was evidently produced in the endocrine glands of nurse bees, which are adapted for needs of bee larvae. In conclusion, this research yielded a surprising and completely new finding--that royal jelly, as a form of lactation on the insect level, shows the same homeostatic adjustment as mammalian and human breast milk. 相似文献
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