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1.
Based on physiological and molecular differences associatedwith the germination of after-ripened and dormant caryopsesand excised embryos, it has been hypothesized that various methodsof after-ripening are the only treatments that facilitate thetransition of dormant wild oat embryos to a non-dormant state.To further investigate this hypothesis, analytical methods wereused to evaluate physical and temporal changes associated withgermination and subsequent growth of after-ripened and dormantexcised embryos (AR-embryos and D-embryos, respectively) inducedto germinate with fructose (Fru) and/or gibberellic acid (GA).While chemical treatments of Fru, GA, and Fru+GA have littleeffect on the germination and short-term growth of AR-embryos,they do induce germination of D-embryos. Growth following germinationof D-embryos varied according to treatment with the combinationof Fru+GA inducing the greatest growth over the duration ofthe experiment. Even considering differences in the time tocomplete germination, growth of D-embryos was not comparablewith that of AR-embryos. This provides physical evidence thatchemical treatments induce germination without fulfilling therequirements for normal after-ripening-enhanced germination/growth,and indicates that fructose and/or gibberellic acid do not removethe dormancy-block or rate limiting step in the same manneras after-ripening. Avena fatua ; after-ripening; dormancy; fructose; germination; gibberellic acid; wild oats 相似文献
2.
Barbara Wciślińska 《Biologia Plantarum》1977,19(5):370-376
The seeds ofFraxinus excelsior L. were stratified at 17-20 °C (warm stratification), at 4-6 °C (cold stratification) and at alternating temperature (warm — cold stratification). The seeds subjected to warm stratification only, remained dormant. The seeds stratified only at 4-6 °C germinated gradually during a long period of time. The seeds subjected to warm — cold stratification, however, germinated with great intensity within a relatively short period of time. GA3 was shown to stimulate the growth of embryos markedly, and its effect on the germination of seeds depended on the temperature of stratification. GA3 applied during the cold stratification accelerated the removal of dormancy by shortening the period of stratification and by influencing the germination of seeds. The results obtained indicate a similarity between the effect of temperature 17-20 °C during the warm stratification and that of gibberellic acid. Both those factors applied separately affect favourably after-ripening of the embryos and accelerate the germination of seeds. 相似文献
3.
The physiological basis of seed dormancy in Avena fatua. IX. Characterization of two dormancy states
The dormancy-breaking effect of several known germination promoters was studied in 9 genetically pure lines of Avena fatua L. during a period of controlled after-ripening. Changes in the germination response show at least two dormancy states in the caryopses of these lines. The first state is overcome by a short period of after-ripening and is insensitive to nitrate and azide, while the second state is more persistent and is sensitive to nitrate and azide. Both states are sensitive to gibberellic acid (OA,) and ethanol. In the most dormant lines a third ethanol-insensitive dormancy state is present. The duration of both major dormancy states was related to several environmental factors influencing plant growth and seed storage. Duration was increased in caryopses produced from plants matured under low temperatures (15°C) and decreased in caryopses produced from plants matured under high temperatures (25°C). Duration was increased in caryopses after-ripened under low temperatures (4°C) and decreased in caryopses after-ripened under high temperatures (45°C). Dehulling the seeds prior to after-ripening reduced the duration of both major dormancy states. The multiple state dormancy system and its environmentally induced plasticity are discussed with reference to previous explanations of the dormancy mechanism in wild oats. 相似文献
4.
T. A. Villiers 《Planta》1968,82(4):342-354
Summary Abscisic acid maintains embryos in a state of dormancy and inhibits the incorporation of H3uridine and H3thymidine but not the incorporation of H3leucine. Ribosomes present in imbibed but dormant embryos do not become associated into polysomes until actual germination of the embryos. Protein synthesis still occurs in embryos when RNA synthesis is inhibited and therefore stable m-RNA must be present in dormant embryos. It is concluded that abscisic acid maintains dormancy by inhibiting the production of specific types of m-RNA, and therefore the formation of specific proteins. The activity of abscisic acid is antagonistic to the effect of gibberellic acid in dormancy. 相似文献
5.
Metabolically active glucosides in oleaceae seeds: I. The effects of germination, growth, and hormone treatments
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The seeds of six woody species of Oleaceae representing three genera, contain high concentrations of water-soluble glucosides, with major absorption maxima below 240 nanometers. In Fraxinus americana seeds three of these compounds, designated GL-3, GL-5, and GL-6, account for almost 10% of the dry weight. They are found in the endosperm and embryo but not in the pericarp. While the level of GL-5 is not particularly influenced by the physiological state of the embryo, that of GL-3 and GL-6 decreases as a result of germination and growth during a 10-day period. As the concentrations of GL-3 and GL-6 decrease, new ultraviolet-absorbing compounds are formed. The changes in the concentration of the ultraviolet-absorbing glucosides during cold temperature after-ripening, prior to germination, are small. When germination of dormant embryos is induced with gibberellic acid, the concentrations of GL-3 and GL-6 decrease in a manner similar to that observed with nondormant embryos. In the presence of abscisic acid no losses of GL-3 and GL-6 were observed. It is suggested that GL-3 and GL-6 fulfill some definite functions in the germination and growth of F. americana embryos, and that gibberellic acid and abscisic acid can exert a regulatory effect on the metabolism of these glucosides. 相似文献
6.
OLAVI JUNTTILA 《Physiologia plantarum》1973,29(2):256-263
The mechanism of seed dormancy at low temperatures (15-9°C) was investigated in the seeds of Syringa josikaea, S. reflexa and S. vulgaris. Low temperature dormancy in Syringa species was mainly imposed by endosperm embedding the radicle. Different degrees of embryo dormancy may occur in S. reflexa seeds. In most cases the low temperature dormancy was broken completely by removing the endosperm around the radicle. The endosperm did not seem to contain significant quantities of germination inhibitors, and the results indicate that it prevents germination mainly due to its mechanical resistance. The mechanical resistance of endosperm did not change during chilling or during induction of dormancy by high temperature incubation. The strength of the endosperm decreased rapidly in non-dormant seeds before visible germination. Similar changes were not observed in dormant seeds. Generally, the strength of the endosperm was lower in the non- (or less) dormant species S. josikaea and S. vulgaris than in the more dormant S. reflexa seeds. The growth potential of the embryos, measured as their ability to germinate in osmotic solutions (mannitol or polyethylen glycol 4000), was increased by chilling and by GA3-treatment. The growth potential of untreated S. josikaea and S. vulgaris embryos was generally higher than that of S. reflexa embryos. Acid ethyl acetate fractions of methanol extracts from embryos of all three species contained substances with GA3-like activity in the lettuce hypocotyl test. The activity was found at Rf 0.9–1.0 on paper chromatograms run in distilled water. No significant changes in the activity were detected during chilling or prior to visible germination. 相似文献
7.
Luzula spicata L. seeds are completely dormant at maturity. A germination inhibitor is present at the micropylar end. Normally, the only effective means of eliciting germination is a precise scarification of the micropylar end which inactivates the inhibitor. Exogenous application of gibberellic acid, kinetin, KNO3, and thiourea have no affect on the dormancy of unscarified seeds. Scarification of the hilar end of the seed does not elicit germination, but when gibberellic acid is applied to the hilar scarified seeds moderate germination results. Presumably, these seeds are dormant due to a deficit of endogenous gibberellin; a condition which can be overcome by the application of gibberellic acid to seeds scarified at a site in itself ineffective in producing germination. Apparently the gibberellic acid serves to initiate amylase activity in the endosperm, overcoming the inhibitor block. Luzula spicata seed dormancy is apparently unique in that a germination inhibitor is operative in conjunction with the commonly recognized gibberellin-amylase mechanism. 相似文献
8.
Activities of RNasea were studied in cotyledons of dormant and afterripenedAgrostemma githago seeds. Activity of RNase increases during imbibition and germination. This increase in activity cannot be observed in variants which are not able to germinate (dormant seeds and seeds blocked by higher temperature). The development of RNase activities during germination cannot be inhibited by concentrations of cycloheximide or actinomycine D completely preventing phosphatase synthesis. These results may be indicative for the assumption that the increase of RNase during germination is caused by enzyme activation and not by enzyme synthesis. Cytokinins and a combination of cycloheximide and gibberellic acid stimulate the activity of RNase in dormant cotyledons, whereas neither cycloheximide nor gibberellic acid, applicated by themselves, show any effect. Cytokinins and gibberellic acid do not influence the activity of RNase of afterripened cotyledons, abscisic acid inhibits the increase of enzyme activity. There are characteristic changes in the pattern of RNases during germination revealed by polyacrylamide gel electrophoresis. The increase in RNase activity of dormant cotyledons caused by cytokinins is accompanied by obvious changes in the RNase pattern on polyacrylamide gel. Treating dormant cotyledons with cytokinins dormancy is partially overcome. In consequence of the application of cytokinins the differences in the electrophoretic RNase pattern between dormant and afterripened cotyledons can be nearly balanced. 相似文献
9.
Summary It was found that the temperature optimum for apple (Malus domestica Borb.) seed acid lipase is the same as that for seed after-ripening process. The activity of the enzyme occurs between the 40th and 70th days of stratification, whereas the activity of alkaline lipase very low at that time appears about 20 days later. The changes of both enzyme activities were also studied during dark and light culture of embryos isolated from seeds after different times of stratification. Only the alkaline enzyme activity is under the control of light. It was concluded that essentially the same process, i.e. the hydrolysis of reserve fats is catalysed by two different enzymes: acid lipase acting during the cold-mediated breaking of embryo dormancy and alkaline lipase acting during the germination of dormant embryos, thus being under light control. 相似文献
10.
Michael E. Foley 《Physiologia plantarum》1986,67(4):690-694
Fusicoccin induced germination in dormant and partially afterripened dormant caryopses of Avena fatua L. The rate of caryopsis germination was slower and final percentage germination lower in the highly dormant inbred line M73 at a given concentration of fusicoccin than in the dormant caryopses of line AN265. Gibberellic acid was more effective than fusicoccin in breaking dormancy in both lines. Promotion of germination of dormant caryopses by fusicoccin was inhibited by a 6-day pretreatment with (2-chloroethyl)trimethylammonium chloride.
The basal rate of proton efflux from embryos isolated from dormant and fully afterripened line AN265 caryopses was similar. Addition of fusicoccin increased the rate of proton efflux from the isolated embryos of dormant and afterripened caryopses by nearly 400%. Gibberellic acid had no effect on the rate of proton extrusion. The uptake of86 Rb+ in dormant and afterripened A. fatua embryos was similar after a 2 h uptake period. The addition of fusicoccin to the medium doubled the uptake of 86 Rb4 by dormant and afterripened embryos. Gibberelleic acid had no effect on the uptake of 86 Rb+ by isolated embryos from either dormant or afterripened caryopses. The experimental results indicate that gibberellic acid is more versatile in its action than fusicoccin, and gibberellic acid may facilitate dormant A. fatua caryopsis germination by stimulating mechanisms other than the direct H+ efflux and K+ uptake at the membrane level. 相似文献
The basal rate of proton efflux from embryos isolated from dormant and fully afterripened line AN265 caryopses was similar. Addition of fusicoccin increased the rate of proton efflux from the isolated embryos of dormant and afterripened caryopses by nearly 400%. Gibberellic acid had no effect on the rate of proton extrusion. The uptake of
11.
Dormancy removal in apple embryos by nitric oxide or cyanide involves modifications in ethylene biosynthetic pathway 总被引:1,自引:0,他引:1
The connection between classical phytohormone-ethylene and two signaling molecules, nitric oxide (NO) and hydrogen cyanide
(HCN), was investigated in dormancy removal and germination “sensu stricto” of apple (Malus domestica Borkh.) embryos. Deep dormancy of apple embryos was removed by short-term (3–6 h) pre-treatment with NO or HCN. NO- or HCN-mediated
stimulation of germination was associated with enhanced emission of ethylene by the embryos, coupled with transient increase
in ROS concentration in embryos. Ethylene vapors stimulated germination of dormant apple embryos and eliminated morphological
anomalies characteristic for young seedlings developed from dormant embryos. Inhibitors of ethylene receptors completely impeded
beneficial effect of NO and HCN on embryo germination. NO- and HCN-induced ethylene emission by apple embryo was only slightly
reduced by inhibitor of 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase activity during first 4 days of germination. Short-term pre-treatment of
the embryos with NO and HCN modified activity of both key enzymes of ethylene biosynthetic pathway: ACC synthase and ACC oxidase.
Activity of ACC synthase declined during first 4 days of germination, while activity of ACC oxidase increased markedly at
that time. Additional experiments point to non-enzymatic conversion of ACC to ethylene in the presence of ROS (H2O2). The results indicate that NO and HCN may alleviate dormancy of apple embryos “via” transient accumulation of ROS, leading
to enhanced ethylene emission which is required to terminate germination “sensu stricto”. Therefore, ethylene seems to be
a trigger factor in control of apple embryo dormancy removal and germination. 相似文献
12.
Dormancy in seeds of hazel (Corylus avellana L.) and beech (Fagussylvatica L.) has been studied with special reference to changesin growth-promoting and inhibiting substances during after-ripening.About 12 weeks at low temperature and under moist conditionsis necessary for complete after-ripening. Gibberellic acid,kinetin, and thiourea stimulate germination in dormant seedsbut have no effect on nuts with the pericarp intact. GibberellinD is ten times more active than gibberellic acid.Bio-assays, following chromatographic fractionation of seedextracts, have revealed no significant changes in the concentrationsof auxins and inhibitors during after-ripening. Dwarf maize-leafsection assays have revealed low concentrations of gibberellin-likesubstances in purified extracts of chilled, dormant hazel seedbut no gibberellin activity in extracts of dormant seed. Gibberellinsare present in both dormant and germinating beech seeds butthere appear to be differences in the chromatographic patternof activity. The possible role of endogenous gibberellins inthe after-ripening process is discussed. 相似文献
13.
Abscisic acid,phaseic acid and gibberellin contents associated with dormancy and germination in barley 总被引:27,自引:0,他引:27
Analyses of abscisic acid (ABA), ent -kaurenoids and gibberellins (GAs) showed that there were major changes in the contents of these compounds associated with germination of after-ripened barley ( Hordeum vulgare cv. Schooner and cv. Proctor) grain but not in hydrated dormant grain. Embryos from dormant and after-ripened dry grain contained similar amounts of ABA, of ent -kaurenoids and of GAs, determined by gas chromatography-mass spectrometry-selected ion monitoring. In embryos of after-ripened grain, ABA content decreased rapidly after hydration and ABA appeared to be metabolized (inactivated) to phaseic acid (PA) rather than diffusing into the endosperm or the surrounding medium as previously thought. Similar changes in ABA occurred in hydrated dormant grain during germination in darkness. Accumulation of ent -kaurenoids and GAs, including GA1, the first biologically active GA in the early 13-hydroxylation biosynthetic pathway, occurred to a much greater extent in after-ripened than in dormant grain and these changes occurred mainly after 18 h of hydration when ABA had already decreased and germination was occurring. The block in ent -kaurenoid and GA synthesis in dormant grain appeared to occur prior to ent -kaurene in the biosynthetic pathway. These results are consistent with the view that ABA is the primary effector of dormancy and that after-ripening involves the development of the ability to reduce the amount of ABA quickly following hydration. Accumulation of GAs does not appear to be causally related to loss of dormancy but it does appear to be related to germination. 相似文献
14.
The physiological basis of seed dormancy in Avena fatua III. Action of nitrogenous compounds 总被引:1,自引:0,他引:1
Sodium nitrate and nitrite (50–100 m M ) induced germination in three out of four genetically pure dormant lines of Avena fatua L. The sensitivity to these treatments was low immediately ater harvest and increased markedly after six months of dry after-ripening. The observation that a fourth dormant line failed to respond suggests at least two metabolic blocks may be involved in expression of dormancy. An inhibitor of gibberellin biosynthesis, 2-chloroethyl trimethylammonium chloride, completely inhibited the dormancy-breaking effect by nitrate and nitrite, indicating a requirement for gibberellin biosynthesis. Among reduced nitrogenous compounds, ammonium chloride and urea failed to break dormancy in all partly after-ripened lines, suggesting that nitrate and nitrite may induce germination through their ability to act as electron acceptors. The sensitivity to all nitrogenous compounds tested increased with the length of after-ripening indicating that the depth of the second dormancy block amy decrease with the time of after-ripening. Other reduced nitrogenous compounds, thiourea and hydroxylamine hydrochloride, promoted some germination in the least dormant, partially after-ripened lines. The function of these compounds as electron acceptors and their similarity in activity to the cytochrome oxidase inhibitor, sodium azide, is discussed with reference to dormancy and the possible involvement of the alternative pathway of respiration. 相似文献
15.
TADASHI FUJII 《Development, growth & differentiation》1969,11(2):153-163
- 1 Both the germination of intact seeds and the development of excised embryos depend on their after-ripening. Shortly after the harvest, the intact seeds of Eragrostis ferruginea are in dormancy and germinate neither in the light nor in the dark. The ability of seeds to germinate under both conditions, however, gradually increases with the progress of after-ripening. The development of excised embryos, on the other hand, is readily induced in the light and dark even shortly after the harvest. As the after-ripening proceeds, however, the ability of excised embryos to develop gradually decreases and is finally lost 18 months after the harvest. The after-ripened embryos whose ability to develop is completely reduced become light sensitive again when they are supplied with the yeast extract of a low concentration, and develop readily even in darkness at a high concentration of the extract.
- 2 The photorequirement for development of excised embryos is scarcely affected by oxygen concentrations, and the embryo growth can take place equally in both light and darkness in the atmosphere containing 100 to 10 percent of air, while it can not be induced in pure nitrogen.
- 3 The promotion of embryo development by light is observed at the time when the ability of embryos to develop declines. The effectiveness of the irradiation is very similar to that in intact seed germination.
16.
A quantitative model for change in germination rate of barley(Hordeum vulgare L.) during and after loss of primary dormancyis presented. Change in mean germination time on a logarithmicscale is normally distributed within the period of after-ripeningand the standard deviation of this distribution is shown tobe a quantitative function of after-ripening temperature. Therate of change of mean germination time is in inverse proportionto the product of the standard deviation and a parameter whichis characteristic of the seed population. The latter parameteris the rate constant for change in probit cumulative germinationas a negative linear function of the logarithm of mean germinationtime. A model based solely on dormancy loss is combined withan existing model of change in probit viability as a functionof mean germination time to produce a model which predicts thetime to and optimum value of mean germination rate of a populationas it after-ripens. The model provides a quantitative link betweenthe effect of pre-germination and germination environments ontotal and rate of germination of an initially dormant population.Experimental data from dormant barley (cv. Triumph) stored at27, 38, 45, 50 and 60 °C, and germinated at 18 °C wereused to validate the model. The data show that germination ratecontinues to increase after primary dormancy is lost until itreaches an upper limit determined by the intrinsic germinativevigour of the seed lot. Rate of loss of primary dormancy andincrease in germination rate thus appear to be quantitativelylinked as a function of after-ripening temperature and factorswhich may be specific to the mode of induction of dormancy withina seed lot prior to harvest.Copyright 1995, 1999 Academic Press Hordeum vulgare L. barley, germination rate model, dormancy, vigour, after-ripening temperature 相似文献
17.
Winter buds of American pondweed (Potamogeton nodosus Poir.)were found to be strongly dormant at maturity. Under normalconditions the dormancy lasted for several months. Cold treatmentdecreased the period required for after-ripening, and exposureof dormant buds to 32° C for three days caused most of thebuds to grow in two weeks. Removal of bud scales in light wasvery effective in promoting growth. Soaking of dormant budsin solutions of gibberellic acid, sucrose, combinations of gibberellicacid and sucrose, -naphthylacetic acid and fenac (2,3,6-trichlorophenylaceticacid) partially relieved the growth suppression. Treatmentswith indole-3-acetic acid at 1,000 ppm for 18 hours broke dormancycompletely in all buds, indicating that low levels of this substancemay be the primary cause of dormancy in this species. 相似文献
18.
Julie A. T. Buchheim Len F. Burkhart Martin M. Meyer Jr. 《Plant Cell, Tissue and Organ Culture》1994,36(1):35-43
Epicotyl dormancy was broken in cultured peony (Paeonia lactiflora Pall.) embryos after topical application of agarose gels containing gibberellic acid, with optimum growth at 1.5 mM gibberellic acid. Addition of 100 M abscisic acid to the medium resulted in complete inhibition of gibberellic acid-stimulated promotion of dormant epicotyls. Epicotyl dormancy was also broken in embryos by culture on media containing 1 or 10 M benzylaminopurine. A highly significant increase in leaf number occurred when embryos were both cultured on medium containing benzylaminopurine and treated topically with gibberellic acid. Anatomical and morphological studies indicated that the increase in shoot growth was due to the development and growth of 1) buds formed at the cotyledonary node, 2) axillary buds, and 3) adventitious meristems originating from subepidermal parenchymatous tissue.Abbreviations ABA
abscisic acid
- BA
N6-benzylaminopurine
- DMSO
dimethyl sulfoxide
- GA3
gibberellic acid
- LS
Linsmaier and Skoog 相似文献
19.
Control of seed dormancy in Nicotiana plumbaginifolia: post-imbibition abscisic acid synthesis imposes dormancy maintenance 总被引:13,自引:0,他引:13
The physiological characteristics of seed dormancy in Nicotiana plumbaginifolia Viv. are described. The level of seed dormancy is defined by the delay in seed germination (i.e the time required prior to
germination) under favourable environmental conditions. A wild-type line shows a clear primary dormancy, which is suppressed
by afterripening, whereas an abscisic acid (ABA)-deficient mutant shows a non-dormant phenotype. We have investigated the
role of ABA and gibberellic acid (GA3) in the control of dormancy maintenance or breakage during imbibition in suitable conditions. It was found that fluridone,
a carotenoid biosynthesis inhibitor, is almost as efficient as GA3 in breaking dormancy. Dry dormant seeds contained more ABA than dry afterripened seeds and, during early imbibition, there
was an accumulation of ABA in dormant seeds, but not in afterripened seeds. In addition, fluridone and exogenous GA3 inhibited the accumulation of ABA in imbibed dormant seeds. This reveals an important role for ABA synthesis in dormancy
maintenance in imbibed seeds.
Received: 31 December 1998 / Accepted: 9 July 1999 相似文献