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The solitary long terminal repeats of ERV-9 endogenous retrovirus are conserved during primate evolution and possess enhancer activities in embryonic and hematopoietic cells
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Ling J Pi W Bollag R Zeng S Keskintepe M Saliman H Krantz S Whitney B Tuan D 《Journal of virology》2002,76(5):2410-2423
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lac repressor forms loops with linear DNA carrying two suitably spaced lac operators. 总被引:57,自引:16,他引:41
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H Krmer M Niemller M Amouyal B Revet B von Wilcken-Bergmann B Müller-Hill 《The EMBO journal》1987,6(5):1481-1491
Tetrameric lac repressor may bind to two lac operators on one DNA fragment and induce the intervening DNA to form a loop. Electron microscopy, non-denaturing polyacrylamide gel electrophoresis, and DNase I protection experiments were used to demonstrate such DNA loops, where the distance between the centres of symmetry of the two lac operators varies between 63 and 535 bp. Formation of a DNA loop is favoured by correct phasing of the two lac operators and a low concentration of both components of the reaction. When a large excess of lac repressor over DNA is used, a 'tandem' structure is observed, in which both lac operators are occupied independently by two repressor tetramers. When the concentrations of both lac repressor and lac operator are high, a 'sandwich' structure is observed, in which two DNA molecules are connected by two lac repressor tetramers in trans. 相似文献
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A family of cloning vectors containing the lacUV5 promoter 总被引:16,自引:0,他引:16
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