Species-specific AFLP markers for identification of Zingiber officinale, Z. montanum and Z. zerumbet (Zingiberaceae)

The Zingiber genus, which includes the herbs known as gingers, commonly used in cooking, is well known for its medicinal properties, as described in the Indian pharmacopoeia. Different members of this genus, although somewhat similar in morphology, differ widely in their pharmacological and therapeutic properties. The most important species of this genus, with maximal therapeutic properties, is Zingiber officinale (garden ginger), which is often adulterated with other less-potent Zingiber sp. There is an existing demand in the herbal drug industry for an authentication system for the Zingiber sp in order to facilitate their commercial use as genuine phytoceuticals. To this end, we used amplified fragment length polymorphism (AFLP) to produce DNA fingerprints for three Zingiber species. Sixteen collections (six of Z. officinale, five of Z. montanum, and five of Z. zerumbet) were used in the study. Seven selective primer pairs were found to be useful for all the accessions. A total of 837 fragments were produced by these primer pairs. Species-specific markers were identified for all three Zingiber species (91 for Z. officinale, 82 for Z. montanum, and 55 for Z. zerumbet). The dendogram analysis generated from AFLP patterns showed that Z. montanum and Z. zerumbet are phylogenetically closer to each other than to Z. officinale. The AFLP fingerprints of the Zingiber species could be used to authenticate Zingiber sp-derived drugs and to resolve adulteration-related problems faced by the commercial users of these herbs.     

Metabolic profiling and phylogenetic analysis of medicinal Zingiber species: Tools for authentication of ginger (Zingiber officinale Rosc)

Phylogenetic analysis and metabolic profiling were used to investigate the diversity of plant material within the ginger species and between ginger and closely related species in the genus Zingiber (Zingiberaceae). In addition, anti-inflammatory data were obtained for the investigated species. Phylogenetic analysis demonstrated that all Zingiber officinale samples from different geographical origins were genetically indistinguishable. In contrast, other Zingiber species were significantly divergent, allowing all species to be clearly distinguished using this analysis. In the metabolic profiling analysis, the Z. officinale samples derived from different origins showed no qualitative differences in major volatile compounds, although they did show some significant quantitative differences in non-volatile composition, particularly regarding the content of [6]-, [8]-, and [10]-gingerols, the most active anti-inflammatory components in this species. The differences in gingerol content were verified by HPLC. The metabolic profiles of other Zingiber species were very different, both qualitatively and quantitatively, when compared to Z. officinale and to each other. Comparative DNA sequence/chemotaxonomic phylogenetic trees showed that the chemical characters of the investigated species were able to generate essentially the same phylogenetic relationships as the DNA sequences. This supports the contention that chemical characters can be used effectively to identify relationships between plant species. Anti-inflammatory in vitro assays to evaluate the ability of all extracts from the Zingiber species examined to inhibit LPS-induced PGE(2) and TNF-alpha production suggested that bioactivity may not be easily predicted by either phylogenetic analysis or gross metabolic profiling. Therefore, identification and quantification of the actual bioactive compounds are required to guarantee the bioactivity of a particular Zingiber sample even after performing authentication by molecular and/or chemical markers.     

Zingiber nanlingensis sp. nov. (Zingiberaceae) from Guangdong,China

Zingiber nanlingensis L. Chen, A. Q. Dong & F. W. Xing sp. nov. (Zingiberaceae) from northern Guangdong, China is described, illustrated, and compared with the morphologically similar species Z. mioga (Thunb.) Rosc.     

Taxonomic studies on Zingiber (Zingiberaceae) in China IV: Z. pauciflorum sp. nov. from Yunnan

Zingiber pauciflorum L.Bai, ?korni?k., D.Z.Li & N.H.Xia (Zingibereae, Zingiberaceae), a new species from southern Yunnan, China, is described and colour plates are provided. It belongs to Z . sect. Cryptanthium Horan. and is compared with three similar species, namely Z. tenuifolium L.Bai, ?korni?k. & N.H.Xia, Z. smilesianum Craib and Z. yunnanense S.Q.Tong & X.Z.Liu. A colour plate of the most similar species, Z. smilesianum is also provided for comparison.     

Five new species of Zingiber (Zingiberaceae) from Borneo

Recent collections from Sabah and Sarawak have revealed a wide species diversity among the Bornean members of the genus Zingiber. Four previously unknown and one incompletely known species of the genus are presented. Z. vinosum is a lowland species characterized by its burgundy colour of the lower surface of the leaves. Z. lambii was collected in primary lowland forest on ultramafic soil while Z. argenteum was collected in primary lowland forest on limestone. Both are characterized by their silvery leaves and orange inflorescences. Z. pendulum, found in primary mountain forest, has pending leafy stems and a deeply split labellum with very well developed side-lobes. Z. latifolium, found in secondary mountain forest, is vegetatively distinct by its broad leaves with a densely pubescent margin Z. vinosum, Z. Iambii, Z. pendulum and Z. latifolium are from Sabah and Z. argenteum is from Sarawak.     

Review of the subgenus Polyphylla (Granida) from continental Asia (Coleoptera, Scarabaeidae, Melolonthinae)

A review of Polyphylla Harris, 1841, species belonging to the subgenus Granida Motschulsky, 1861, from continental Asia is presented. One new species is described from Thailand: Polyphylla (Granida) simonisp. n.Polyphylla (Granida) nikodymi de Wailly, 1993, is recorded from Thailand for the first time. Polyphylla (Granida) minor Nomura, 1977, is recorded from Yunnan (China) for the first time. The previously unknown female of Polyphylla (Granida) phongsali Zídek, 2006, is described.     

Taxonomic studies on Zingiber (Zingiberaceae) in China VI: Z. leucochilum,a new species with running rhizome from Sichuan

Zingiber leucochilum L.Bai, ?korni?k. & N.H.Xia (Zingibereae, Zingiberaceae), a new species from southeastern Sichuan, China, is described and illustrated with a colour plate. Notes on ecology, distribution and a preliminary IUCN conservation assessment are also provided. The new species is assigned to Z . sect. Cryptanthium Horan. and compared to five morphologically similar species from China which share the characteristic well‐elongated and running rhizome, namely, Z. emeiense Z.Y.Zhu, Z. leptorrhizum D.Fang, Z. pauciflorum L.Bai, ?korni?k., D.Z.Li & N.H.Xia, Z. smilesianum Craib and Z. yunnanense S.Q.Tong & X.Z.Liu. A key to the above six species is provided and their distributions are mapped.     

十种姜科植物的染色体数目研究

报道了姜科3属10种植物的染色体数目。(1)姜属5种:版纳姜2n=22,弯管姜2n=22,圆瓣姜2n=22,红球姜2n=22,紫色姜2n=22;(2)舞花姜属4种:舞花姜2n=24,毛舞花姜2n=48,双翅舞花姜2n=48,澜沧舞花姜2n=32;(3)凹唇姜属1种:白斑凹唇姜2n=36。其中弯管姜、圆瓣姜、紫色姜、澜沧舞花姜、白斑凹唇姜的染色体数目为首次报道。     

Two new species of Zingiber (Zingiberaceae) from the Philippines

Zingiber banahaoense and Z. matutumense from the Philippines are described and illustrated.     

A new species of Zingiber (Zingiberaceae) from Vietnam

Zingiber collinsii , a new species from Vietnam is described and illustrated. It is placed in section Zingiber having the inflorescence on a radical, short erect scape.     

Six new species of Zingiber (Zingiberaceae) from Borneo

Six new species of the genus Zingiber from Borneo are presented. Z viridiflavum was collected in primary lowland forest, while Z chlorobracteatum was collected in hilly secondary forest. Both are characterized by their slender fusiform inflorescences with yellow-green bracts. Z. flagelliforme found in primary lowland forest displays a peculiar reproduction habit in its tapering leafy shoots which bend over to trail on the ground where plantlets produced in the leaf-axis take root. Z velutinum was found in mid-elevation secondary forest. It is a tall plant with large ovate inflorescences and bright red, densely hairy bracts. Z phillippsii was found in a gulley in montane primary forest. It is vegetatively characterized by its purple stems and glabrous leaves while the inflorescence has a similarity to the common Bornean species Z. coloratum. Z. georgeii is distinct by its procumbent inflorescences with orange bracts. Z viridiflavum, Z. chlorobracteatum, Z. flagelliforme, Z. phillippsii and Z velutinum are from Sabah and Z georgeii is from Sarawak.     

The contribution of Jonathan Pereira (1804 – 1853) to plant taxonomy

The contribution of Jonathan Pereira to plant taxonomy is reviewed. He described three species in the course of his extensive publications on materia medica. All three are members of the Zingiberaceae. Elettaria cardamomum-zeylanicum J. Pereira from Sri Lanka has been completely overlooked since its publication. It is a synonym of Elettaria cardamomum var. major Baker. The West African Amomum citratum J. Pereira is correctly known as Aframomum citratum (J. Pereira) K. Schum. Amomum korarima J. Pereira is a synonym of Aframomum corrorima (A. Braun) P. C. M. Jansen, an important spice-plant from Ethiopia. The typification of these taxa and their synonyms is reviewed. This includes new lectotypifications for two species described by Gaertner, Zingiber ensal and Z. minus. A note concerning the valid publication of 14 nomenclatural novelties by B. A. Abeywickrama is included.     

Biocontrol activity of the extract prepared from Zingiber zerumbet for the management of rhizome rot in Zingiber officinale caused by Pythium myriotylum

Zingiber zerumbet Smith or wild ginger is remarkable for its inherent resistance to Pythium spp., which cause soft rot disease in Zingiber officinale Rosc. In the present study, various concentrations of extract prepared from Z. zerumbet were screened for its activity against Pythium myriotylum. Microscopic observation of P. myriotylum in presence of Z. zerumbet extract has confirmed the complete lysis of pathogen within 10 h. However, the same treatment with Z. officinale extract was found to have partial antifungal effect even after 24 h due to inability of its metabolites to prevent the growth of P. myriotylum. Due to the antifungal activity, extract from Z. zerumbet was subjected to GC–MS and LC-QTOF-MS which has identified Zerumbone with m/z 219 as the major compound. Further, in vivo study and the subsequent microscopic analysis have confirmed the applicability of extract from Z. zerumbet as a phytomedicine to control rhizome rot in ginger.     

The distribution of intertidal algae in a Hebridean Loch

Summary

Ten species of Rhopalostroma are currently known and all are from either Africa or Asia. Rhopalostroma gracile D. Hawksw. and R. kanyae Whalley &; Thienhirun have previously been reported from Thailand. A further species, R. lekae sp. no v., is now described from Thailand. Thus three out of the eleven known species of Rhopalostroma are from Thailand.     

Population genetic structure of Zeugodacus tau species complex in Thailand

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Morphological analysis of vessel elements for systematic study of three Zingiberaceae tribes

Zingiberaceae containing over 1,000 species that are divided into four subfamilies and six tribes. In recent decades, there has been an increase in the number of studies about vessel elements in families of monocotyledon. However, there are still few studies of Zingiberaceae tribes. This study aims to establish systematic significance of studying vessel elements in two subfamilies and three tribes of Zingiberaceae. The vegetative organs of 33 species processed were analysed by light and scanning electron microscopy and Principal Component Analysis was used to elucidate genera boundaries. Characteristics of vessel elements, such as the type of perforation plate, the number of bars and type of parietal thickening, are proved to be important for establishing the relationship among taxa. Scalariform perforation plate and the scalariform parietal thickening are frequent in Zingiberaceae and may be a plesiomorphic condition for this taxon. In the Principal Component Analysis, the most significant characters of the vessel elements were: simple perforation plates and partially pitted parietal thickening, found only in Alpinieae tribe, and 40 or more bars composing the plate in Elettariopsis curtisii, Renealmia chrysotricha, Zingiber spectabile, Z. officinale, Curcuma and Globba species. Vessel elements characters of 18 species of Alpinieae, Zingibereae and Globbeae were first described in this work.     

Platelet-activating factor (PAF) receptor-binding antagonist activity of Malaysian medicinal plants

Forty-nine methanol extracts of 37 species of Malaysian medicinal plants were investigated for their inhibitory effects on platelet-activating factor (PAF) binding to rabbit platelets, using 3H-PAF as a ligand. Among them, the extracts of six Zingiberaceae species (Alpinia galanga Swartz., Boesenbergia pandurata Roxb., Curcuma ochorrhiza Val., C. aeruginosa Roxb., Zingiber officinale Rosc. and Z. zerumbet Koenig.), two Cinnamomum species (C. altissimum Kosterm. and C. pubescens Kochummen.), Goniothalamus malayanus Hook. f. Momordica charantia Linn. and Piper aduncum L. are potential sources of new PAF antagonists, as they showed significant inhibitory effects with IC50 values ranging from 1.2 to 18.4 microg ml(-1).     

Taxonomic changes regarding three species of Zingiber (Zingiberaceae) from Thailand

Zingiber teres S. Q. Tong & Y. M. Xia and Z. xishuangbannaense S. Q. Tong are reduced to be synonyms of Z. smilesianum Craib and Z. thorelii Gagnep.,respectively. Z. cochinchinense Gagnep. is changed to be a subspecies of Z. zerumbet (L.) Sm.     

Pollen morphology of the genus Cornukaempferia (Zingiberaceae) in Thailand

Cornukaempferia is a recently described genus of Zingiberaceae which only occurs in Thailand as a rare genus with limited geographical distribution. Only three species have been described so far, including a recently described new species, C. larsenii. These three species are morphologically very similar and additional data on other biological aspects are useful for the elucidation of their relationship. Pollen morphology of all three species of genus Cornukaempferia has been studied by light microscopy and scanning electron microscopy. The pollen grains are monad, spherical, inaperturate. The exine sculpture is echinate with psilate between the spines for C. aurantiflora and C. longipetiolata, or echinate with regulate between the spines for C. larsenii. This obser-vation helps support the taxonomic status of C. larsenii.