共查询到20条相似文献,搜索用时 31 毫秒
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T J Berrodin M S Marks K Ozato E Linney M A Lazar 《Molecular endocrinology (Baltimore, Md.)》1992,6(9):1468-1478
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J Iyer D Bonnier F Granger C Iltis V Andry P Schultz H Gronemeyer 《Protein expression and purification》1999,16(2):308-314
RAR-RXR heterodimeric complexes (RARalphaDeltaAB-RXRalphaDeltaAB) bound to cognate DR5 DNA response elements were purified to apparent structural and functional homogeneity using a novel versatile immobilized metal affinity chromatography (IMAC) copurification procedure. Dynamic light scattering studies indicated that the complexes were more than 85% monodisperse. By electron microscopy the negatively stained RAR-RXR-DNA complexes appeared homogeneous and corresponded to a dimeric arrangement of the molecules. Using heterodimers purified according to this procedure we demonstrate ligand binding of RXR in the context of the RAR-RXR heterodimer-DNA complex. The present copurification procedure is rapid and has yielded high quality heterodimer-DNA complexes suitable for both structural and biochemical studies. 相似文献
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COUP orphan receptors are negative regulators of retinoic acid response pathways. 总被引:13,自引:11,他引:13 下载免费PDF全文
P Tran X K Zhang G Salbert T Hermann J M Lehmann M Pfahl 《Molecular and cellular biology》1992,12(10):4666-4676
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Ribeiro RC Feng W Wagner RL Costa CH Pereira AC Apriletti JW Fletterick RJ Baxter JD 《The Journal of biological chemistry》2001,276(18):14987-14995
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