共查询到20条相似文献,搜索用时 15 毫秒
1.
J. D. Tan 《Journal of fish biology》1985,26(6):657-668
The pituitary gland of the milkfish, Chanos chanos , was studied at different stages of sexual maturation and spawning. Consecutive median sagittal sections were treated with a range of stains to demonstrate the different cell types and regions. The milkfish pituitary consists of a neural component, the neurohypophysis, and an epithelial component, the adenohypophysis, which in turn consists of three regions: the rostral pars distalis (RPD), proximal pars distalis (PPD), and pars intermedia (PI). However, unlike most teleosts, the pituitary gland of the milkfish is encased in a bony chamber, has dorsal and ventral lobes and extends anteriorly from its point of origin at the base of the brain. PAS (+) basophils are found in all regions of the adenohypophysis, but mostly in the proximal pars distalis. These cells undergo hypertrophy and hyperplasia during sexual maturation, shrinkage and degranulation during spawning. 相似文献
2.
In the pituitary of A. testudineus the rostral pars distalis (RPD), proximal pars distalis (PPD) and pars intermedia (PI) are arranged in a rostro-caudal axis. The rostral neurohypophysis (NH) extends dorsal to the pars distalis, while the caudal part vertically penetrates into the PI and ramifies in its component. The RPD mainly consists of erythrosinophils and PgH-positive cells which are comparable to the lactotropes and corticotropes mentioned in the literature. In the nonbreeding season, acidophils are the predominant cells of the PPD and are largely confined to its dorsal aspect. The two types of cyanophils present in the PPD could not be differentiated with the various techniques used in this study. However, those cyanophils which increase in number and are active during the spawning season may be the gonadotropes, while those which are cytologically inactive may be the thyrotropes. The pars intermedia consists of PAS+ and PhH+ cells. Trichrome and tetrachrome staining techniques revealed the presence of acidophils, cyanophils and amphiphils in the PI. 相似文献
3.
Weltzien FA Norberg B Helvik JV Andersen Ø Swanson P Andersson E 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2003,134(2):315-327
The eight distinct hormone-producing cell types in the adenohypophysis of male Atlantic halibut (Hippoglossus hippoglossus L.) were identified and localized using immunohistochemistry and in situ hybridization. Lactotropes either occupied most of the rostral pars distalis (RPD) or they were arranged in follicular structures located along the periphery of the RPD. Corticotropes were confined to a thin layer of RPD cells bordering the pars nervosa (PN). The somatotropes were arranged in multicellular layers bordering the highly convoluted PN penetrating the proximal pars distalis (PPD), while thyrotropes, scattered in small islets in between the somatotropes, were located in the centro-dorsal part of the PPD. Gonadotropes were found throughout the PPD. Immunoreactivity to glycoprotein-alpha and luteinizing hormone beta-subunit was also observed along the periphery of the pars intermedia (PI), indicating that a thin extension of the PPD surrounded the PI. In situ hybridization showed that follicle-stimulating hormone and luteinizing hormone were produced in distinct cells of the PPD. PI contained somatolactotropes bordering the highly convoluted PN, and melanotropes that showed positive immunostaining against both anti-alpha-melanocyte-stimulating hormone and anti-beta-endorphin. The general cellular organization was similar to that of other teleost fish. These results lay the basis for future investigations on Atlantic halibut pituitary physiology. 相似文献
4.
Nozaki M Ominato K Takahashi A Kawauchi H Sower SA 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》2001,129(2-3):303-309
Adenohypophysial cell types in the pituitary of adult sea lampreys, Petromyzon marinus, was localized by means of immunocytochemical and lectin cytochemical techniques. At least four types of adenohypophysial hormone cells are present in the pituitary of adult sea lampreys. The first type of cell is ACTH-like and occupies most parts of the rostral pars distalis (RPD), but a few scattered ACTH-like cells are also present in the proximal pars distalis (PPD). The second type of cell is MSH-like and occupies the whole pars intermedia. The third type of cell is GH/PRL-like and occupies the dorsal half of the PPD. These GH/PRL-like cells were initially detected by heterologous immunocytochemistry using antibodies to salmon GH, salmon PRL and blue shark GH, after hydrated autoclave pretreatment of sections. Later, by use of an antiserum raised against a synthetic peptide corresponding to the partial sequence of lamprey GH/PRL, the same cells as those containing GH/PRL-like immunoreactivity were stained positively. Similarity of the topographic distributions between lamprey GH/PRL-like cells and gnathostome fish GH cells in the pituitary suggests that GH/PRL-like cells in the lamprey may be GH cells. The last type of cell is GTH-like and occupies the ventral half of the PPD. Although GTH has not yet been isolated from the lamprey pituitary, our immunocytochemical data suggest that GTH-like material in the sea lamprey pituitary is more closely related to mammalian-like LH, rather than to FSH or TSH. These four types of adenohypophysial cells occupy most parts of the lamprey adenohypophysis and indeed there is little room for TSH or PRL cells. Thus, the present study further suggests that GH and LH-like GTH are ancestral forms of GH/PRL/SL family and glycoprotein hormones, respectively. 相似文献
5.
S Haider 《Endokrinologie》1975,65(3):300-307
The pituitary of Heteropneustes fossilis is divisible into rostral (RPD) and proximal (PPD) pars distalis, and pars intermedia (PI). Neurohypophysis extensively ramifies in the PI. The RPD is formed of acidophilic prolactin cells and lead hematoxylin (H. Pb) positive ACTH cells. The latter is arranged around the neurohypophysial extensions. The PPD is comprised of thyrotrophs (TSH) and gonadotrophs which are stainable with PAS, AF, and aniline blue. Acidophilic somatotrophs (STH) and chromophobes are scattered throughout the PPD. In the pars intermedia PAS positive and H. Pb positive cells are present. The latter is believed to secrete MSH whereas the function of the former is yet to be confirmed. In the partly and completely thyroidectomised H. fossilis the TSH cells exhibited marked hypertrophy. They are located in the middle of the PPD flanked by the gonadotrophs on either side. Hypertrophy of the ACTH cells were also evident. But the other cell types did not show any marked changes as compared to the controls. 相似文献
6.
The adenohypophysis of the greater weever fish (Trachinus draco) was studied using histochemical and immunocytochemical methods. The adenohypophysis comprised the rostral pars distalis (RPD), the proximal pars distalis (PPD), and the pars intermedia (PI). Neurohypophysis showed a patent hypophyseal stalk which was divided into several branches intermingled with the adenohypophysis. Salmon prolactin (PRL)-immunoreactive (ir) cells, arranged in follicles, resided in the RPD and the most rostral part of the ventral PPD. Human adrenocorticotropin (ACTH)-ir cells were located in the RPD between PRL-ir cells and the neurohypophyseal processes. Salmon and seabream somatotropin (GH)-ir cells were located in both the dorsal and the ventral PPD. Some GH-ir cells were seen in surrounding and in contact with neurohypophyseal branches, whereas other isolated or clustered GH-ir cells were embedded in adenohypophyseal cells of the PPD. In addition, isolated or clustered GH-ir cells were also detected in the tissue of the PPD covering the most rostral part of PI. Only one class of salmon and carp gonadotropin (GTH)-ir cells was detected. Isolated or clustered GTH-ir cells resided in both the dorsal and the ventral PPD and were seen surrounding the PI and in the tissue of the PPD covering the most rostral part of PI. In addition, a few scattered GTH-ir cells were observed in the ventral RPD. Scattered groups of thyrotropin (TSH)-ir cells were present in the anteroventral PPD. Salmon and seabream somatolactin (SL)-ir and bovine melanotropin (MSH)-ir cells were intermingled surrounding the neurohypophyseal tissue. SL-ir cells were negative to periodic acid-Schiff technique. MSH-ir cells showed a very weak immunoreactivity to anti-human ACTH((1-24)) serum. In addition to the PI location, few isolated or clustered SL- and MSH-ir cells were observed in the dorsal PPD. 相似文献
7.
The RPD (rostral pars distalis) is formed of two cell types. The erythrosinophilic acidophils were hypertrophied in response to ammonium sulphate treatment and dark background adaptation. The response to former may be due to osmoregulatory involvement. The PbH positive corticotrophs were markedly hypertrophied and degranulated in response to metopirone and ammonium sulphate treatments. They were moderately activated in the dark and white background adapted fish. The PPD (proximal pars distalis) is composed of two types of cyanophils and acidophils. The thyrotrophs are identified by their hypertrophic response to radiothyroidectomy and thiourea treatment. They are distributed in the ventral part of the PPD, posterior neurohypophysis and PI (pars intermedia). The gonadotrophs which largely occupy the dorsal aspect of the PPD increase numerically as the gonads mature and become the major cell type before spawning. The acidophilic somatotrophs are distributed all over the PPD. They are the predominant cell type in the PPD of fish with immature gonads. They gave strong reaction to Baker's acid haematein indicating the presence of phospholipid. Both PbH and PAS cells of PI were stimulated in fish kept in dark background whereas they did not exhibit any obvious change under white background adaptation. 相似文献
8.
9.
van Putten Leonie J. A. van Oordt P. G. W. J. Terlou M. Peute J. 《Cell and tissue research》1983,231(1):185-198
Alcian blue- and periodic acid-Schiff-positive "granular basophils" with electron-dense granules +/- 160 nm in diameter, and weakly developed irregular cisternae of the granular endoplasmic reticulum (GER) are present in the rostral pars distalis (RPD) and the proximal pars distalis (PPD) of immature rainbow trout. They all react with antisalmon alpha beta-gonadotropin. However, only granular basophils in the caudal RPD and the rostro-dorsal PPD, often bordering on and sometimes surrounded by neurophypophysial tissue, react with anti-human beta-TSH. These cells, considered as the source of thyrotropin, show degranulation and dilatation of the GER-cisternae in fish treated with potassium perchlorate. The thyroids of the goitrogen-treated animals had relatively numerous small follicles with a high epithelium. The remaining granular basophils are gonadotrops. 相似文献
10.
The pituitary gland of Puntius sarana is of the leptobasic type and has three subdivisions--a proximal pars distalis (PPD), a rostral pars distalis (RPD) and a pars intermedia (PI) arranged vertically one below the other. The gonadotrophs (GTH cells) of the PPD were identified by their PAS, ATh, AF, aniline blue and alcian blue positivity and by their immunoreactivity to ovine anti-LH beta-subunit, anti-salmon GTH and anti-silver carp GTH. The testes of Puntius sarana have been classified as the "unrestricted type". The morphological, histological and histometric findings indicate that the testing passes through four distinct stages--preparatory, fully mature, spawning and spent. Spermatogenesis begins in January and continues up to July. The spawning season lasts from late July until October. Cyclical changes in the gonadotrophs correlate well with changes in the testes. The histometric data show that the GTH cells attain maximum values (size and number) during the breeding season. The peak values for testicular weight, lobule diameter, the gonadosomatic index (GSI) and the sperm concentration in the testes, and the number, size and amount of secretory material in the cytoplasm of the gonadotrophs, coincide with the time of maximum environmental temperature and the longest days. 相似文献
11.
The pituitary gland of Latimeria chalumnae is situated rostroventral to the telencephalon. The hollow pituitary stalk is bent forward and is ventrally connected to a saccus-vasculosus-like organ, rostrally to a neurointermediate lobe. The infundibular lumen protrudes far into the neurohypophysial lobules. The elongated principal part (pars cerebralis) of the pars distalis is partly embedded in a dorsal depression of the pars intermedia and caudally invaded by the neurohypophysis. It may be divided into rostral and proximal pars distalis and includes a ramified hypophysial cleft, which continues rostrally as a duct with adjacent islets of pars distalis tissue (parts of a pars buccalis). The adenohypophysis consists of cell cords and follicles. Eight tinctorial cell types can be distinguished: in the rostral islets: large basophils with acidophil globules, in the rostral pars distalis: small basophils, large basophils with amphiphil characters and erythrosin-, orange G-positive acidophils; in the proximal pars distalis: orange G-positive acidophils and small and large basophils, having similar staining properties; in the pars intermedia: one amphiphil cell type. 相似文献
12.
Rodríguez-Gómez FJ Rendón-Unceta MC Piñuela C Muñoz-Cueto JA Jiménez-Tenorio N Sarasquete C 《Histology and histopathology》2001,16(2):443-451
In this paper we report the first complete mapping of the pituitary in a tuna species. The various different adenohypophysis cell types of the bluefin tuna, Thunnus thynnus L. have been identified and located using different antisera against mammalian and piscine hormones and various histochemical techniques: PAS, Alcian Blue pH 2.5 and lectins -ConA and WGA(Neutral and Acidic Glycoproteins); Bromophenol Blue (Proteins) and Tioglycollate-Ferric-Ferricianide-FeIII (-S-S- groups). Prolactin (PRL) and adrenocorticotrophic (ACTH) cells were located in the rostral pars distalis (RPD) of the pituitary, while the proximal pars distalis (PPD) displayed gonadotrophic (GTH), thyrotrophic (TSH), somatotrophic (GH) and also a few PRL cells. Moreover, somatolactin (SL) and melanotrophic (MSH) cells were identified inside the pars intermedia (PI). Interestingly, some SL-immunoreactive fibers were also detected in the neurohypophysis. Some GTH cells were also located on the exterior surface of the PI. Glycoproteins containing mannose (Man) and/or glucose (Glc); N-acetyl-glucosamine (GlcNAc) and/or sialic acid sugar residues, as well as -S-S- groups, were observed in GTH, TSH and SL cells. The Bromophenol Blue technique stained amphiphilic SL, acidophilic GH cells and weakly ACTH cells. GH and ACTH cells were unreactive to PAS, Alcian Blue, Tioglycollate-Ferric-Ferricianide-FeIII and lectin (Con A and WGA) techniques. Finally, PAS reaction was positive in amphiphilic SL cells, which were PbH unreactive, while MSH and ACTH cells were stained with PbH technique. 相似文献
13.
The pituitaries of the exotic carp (Carassius carassius) are studied at the light microscopic level, for the characterization of the adenohypophysial cell-types with particular emphasis to the gonadotropic potency of the pituitary in relation to the annual reproductive patterns. The gland in the fish is of the cranioleptobasic type. Based on the classical staining methods and localization of the differential cell-types, the adenohypophysis of the fish can be subdivided into the rostral (RPD) and proximal (PPD) pars distalis and the pars intermedia (PI). The pars nervosa (PN) of the fish consists of the neurosecretory (NS) fibres which show varied accumulations of the NS material in concert with the seasonal reproductive cycle. The NS tract is constituted of AF--ve nucleus praeopticus and AF + ve nucleus lateralis tuberis. 2 tinctorially different cell-types, corresponding to corticotrops and lactotrops of other teleosts, are identifiable in the RPD of the present species. In the PPD, 3 cell-types can be distinguished with various granule-staining procedures: they represent somatotrops, thyrotrops, and gonadotrops. Further, 2 gonadotropic cells with apparently different staining properties are discernible. In the PI are dispersed besides a few agranular cells, islets of 2 kinds of cells separable with combined PAS-PbH technique. Seasonal changes in the gonadosomatic index and diameter of the testicular lobules and egg diameter as indices of gonadal maturation are found to be closely interwoven with the morphometric alterations in the basophilic cell area (BCA) chosen as criterion of gonadotropic potency of the PPD. The maximum BCA values are preceded with the time of greatest atresia of the eggs indicating the influence of the pituitary gonadotropins in the follicular resorption in the test fish. 相似文献
14.
The present communication deals with a histological study of the pituitary gland of the teleost fish Mugil cephalus , found in the estuarine waters of Cochin area. Six different cell types were identified in the pituitary gland on the basis of their grouping, distribution and staining properties. The prolactin and the TSH cells (thyroid stimulating hormone producing cells or thyrotrops) were identified in the rostral pars distalis and the ACTH cells (adrenocorticotropic hormone producing cells or corticotrops) in the interphase between the neurohypophysis and the rostral pars distalis. The STH cells (somatotropic hormone producing cells or somatotrops) and the gonadotropic cells were distinguished in the proximal pars distalis and the MSH cells (melanin stimulating hormone producing cells or melanotrops) in the pars intermedia. 相似文献
15.
Prof. Mordechai Abraham Martin Kieselstein Volker Hilge Shoshana Lison 《Cell and tissue research》1982,225(3):567-579
Summary Extravascular circulation in the pituitary of Mugil cephalus was investigated by injecting live fish with horseradish peroxidase and studying the distribution of the enzyme in the gland. The principal components of the extravascular circulatory system are the pericapillary spaces, and, arising from them, the interlobular and circumhypophyseal spaces. Extensions of these spaces penetrate the glandular parenchyma of the pars distalis, where they merge with pericellular spaces. In the neurohypophysis, pericapillary spaces are connected to the periaxonal spaces.Capillaries penetrating from the proximal neurohypophysis into the pars distalis are accompanied by neurosecretory axons. These axons form a mass of tissue which is limited near the capillaries by the pericapillary spaces and near the adenohypophysis by the interlobular spaces. Toward the interior of the adenohypophysis the amount of nervous tissue accompanying the capillaries progressively diminishes, thus reducing the distance between pericapillary and interlobular spaces. Within the pars distalis, the neurosecretory axons accompanying the capillaries are sparse, and the secretory and stellate cells are mostly located directly adjacent to the pericapillary spaces. In the neuro-intermediate lobe, interlobular spaces outline the neuro-adenohypophyseal boundary.The relationship between extravascular spaces and hormone-secreting cells varies in the different regions of the adenohypophysis depending upon the type of neurosecretory innervation in the respective region. In the regions of prolactin and gonadotropin cells, where neurosecretory axons are in direct contact with the secretory cells, the hormone-secreting and stellate cells are adjacent to the pericapillary spaces. In the regions of ACTH and STH cells, secretory and stellate cells are found adjacent to the interlobular spaces, which are interposed between the cells and the neurosecretory axons.Abbreviations AH
adenohypophysis
- CH
circumhypophyseal
- DNH
distal neurohypophysis
- HRP
horseradish peroxidase
- NH
neurohypophysis
- NS
neurosecretory
- PD
pars distalis
- PI
pars intermedia
- PPD
proximal pars distalis
- RNH
rostral neurohypophysis
- RPD
rostral pars distalis
This research was supported by a grant from the National Council for Research and Development, Israel, and the GKSS Geesthacht-Tesperhude, Federal Republic of Germany 相似文献
16.
草鱼催乳素抗血清的制备与鉴定 总被引:1,自引:0,他引:1
应用从草鱼垂体中分离纯化的催乳素免疫兔子制备了特异抗血清。用常规酶联免疫吸附测定法检测表明该抗血清与马哈鱼生长激素及促性腺激素完全没有交叉反应,而与草鱼生长湟级向弱交叉反应。免疫细胞化学染色表明该抗血清主要与草鱼垂体前叶催乳素细胞发生结合反应,与垂体间叶细胞有微弱染色反应,但与神经垂体琢垂体后叶细胞则完全没有免疫结合反应。 相似文献
17.
Dr. M. Olivereau F. Ollevier F. Vandesande J. Olivereau 《Cell and tissue research》1984,238(2):289-296
Summary Immunocytochemical investigations show that somatostatin (SRIF)-like immunoreactive material is present in the brain and the pituitary of nine different species of teleosts. In the brain, immunoreactive perikarya and fibers are observed in the preoptic periventricular nucleus, the entopeduncular nucleus, the anterior periventricular nucleus, and the nucleus lateralis tuberis. In the pituitary, SRIF-like-immunoreactive fibers occur in the proximal pars distalis (PPD), which contains the growth hormone (GH)-secreting cells. Nerve fibers are scattered among GH cells (cyprinids), or end on the basal lamina at the neuroglandular interface of the PPD (eel, salmonids). In the eel, the proximal neurohypophysis does not penetrate deeply into the PPD that is very poorly vascularized. In some species, e.g. Myoxocephalus, SRIF-like immunoreactive fibers are also observed in the caudal neurohypophysis, and even among MSH cells of the pars intermedia.In long-term starved carps and eels, the amount of SRIF-like material in the pituitary is clearly reduced. A possible role of SRIF in the concomitant stimulation of GH cells is discussed. 相似文献
18.
Fish pituitary plays a central role in the control of growth, development, reproduction and adaptation to the environment. Several types of hormone-secreting adenohypophyseal cells have been characterised and localised in diverse teleost species. The results suggest a similar distribution pattern among the species investigated. However, most studies deal with a single hormone or hormone family. Thus, we studied adjacent sections of the pituitary of Oreochromis niloticus, the tilapia, by conventional staining and immunohistochemistry with specific antisera directed against growth hormone (GH), prolactin (PRL), somatolactin (SL), thyrotropin (beta-TSH), follicle-stimulating hormone (beta-FSH), luteinising hormone (beta-LH), adrenocorticotropic hormone (ACTH) and melanocyte-stimulating hormone (alpha-MSH). The pituitary was characterised by a close interdigitating neighbourhood of neurohypophysis (PN) and adenohypophysis. PRL-immunoreactive and ACTH-immunoreactive cells were detected in the rostral pars distalis. GH-immunoreactive cells were present in the proximal pars distalis (PPD). A small region of the PPD contained beta-TSH-immunoreactive cells, and beta-LH-immunoreactive cells covered approximately the remaining parts. Centrally, beta-FSH-immunoreactive cells were detected in the vicinity of the GH-containing cells. Some of these cells also displayed beta-LH immunoreactivity. The pars intermedia was characterised by branches of the PN surrounded by SL-containing and alpha-MSH-immunoreactive cells. The ACTH and alpha-MSH antisera were observed to cross-react with the respective antigens. This cross-reactivity was abolished by pre-absorption. We present a complete map of the distinct localisation sites for the classical pituitary hormones, thereby providing a solid basis for future research on teleost pituitary. 相似文献
19.
The vertebrate pituitary gland is a key endocrine control organ that contains six distinct hormone secreting cell types. In this study, we analyzed the role of direct cell-to-cell Delta-Notch signaling in zebrafish anterior pituitary cell type specification. We demonstrate that initial formation of the anterior pituitary placode is independent of Notch signaling. Later however, loss of Notch signaling in mind bomb (mib) mutant embryos or by DAPT treatment leads to increased numbers of lactotropes and loss of corticotropes in the anterior pars distalis (APD), increased number of thyrotropes and loss of somatotrope cell types in the posterior pars distalis (PPD), and fewer melanotropes in the posterior region of the adenohypophysis, the pars intermedia (PI). Conversely, Notch gain of function leads to the opposite result, loss of lactotrope and thyrotrope cell specification, and an increased number of corticotropes, melanotropes, and gonadotropes in the pituitary. Our results suggest that Notch acts on placodal cells, presumably as a permissive signal, to regulate progenitor cell specification to hormone secreting cell types. We propose that Notch mediated lateral inhibition regulates the relative numbers of specified hormone cell types in the three pituitary subdomains. 相似文献
20.
Dr. J. N. Fryer 《Cell and tissue research》1981,214(2):387-395
Summary The cytology of the growth-hormone (GH) cells of the goldfish pituitary were examined following electrothermic lesions of the anterior praeoptic hypothalamus and telencephalon. Following lesions of the nucleus preopticus (NPO) light microscopy of the pituitary revealed a significant increase in the nuclear diameter and a degranulation of the GH cells. Lesions of the telencephalon anterior or dorsal to the NPO had no cytological effect on the GH cells. The ultrastructural appearance of the GH cells of NPO-lesioned fish was characterized by a marked degranulation of the cytoplasm and a proliferation of the rough endoplasmic reticulum indicative of enhanced secretory activity. The GH cells of the proximal pars distalis (PPD) are directly innervated by peptidergic (type A) and aminergic-like (type B) neurosecretory axons. Following lesions of the NPO, there was a marked reduction in the number of type A fibers in the PPD. These results suggest that the type A fibers innervating the GH cells originate in the NPO and act to inhibit the secretory activity of the GH cells. 相似文献