Parabiotic development of fused eggs from the Hymenopteron,Pimpla turionellae,and of eggs injected with energids

Explanted oocytes and eggs of different developmental stages from the Hymenopteron Pimpla were fused in pairs as parabiotic tandems. Interactions within the tandem were analysed by time lapse films. Except for the exchange of nuclei, no joint development was observed, and each partner followed its own time pattern. The rapid cell cycles of the cleavage energids switched over to longer cycles according to the local developmental stage of the different egg regions, although all of the nuclei were still contained in a single plasmodium. In a second experimental series, nuclei in newly deposited eggs were X-rayed and replaced by cleavage energids from later stages injected into the wrong (=posterior) egg pole. Even injected blastoderm nuclei immediately took up mitotic activities and underwent rapid cell cycles characteristic of early cleavage. Normal embryos could be formed, although the nuclei had populated the egg in a reverse direction.     

Pattern formation fails after blastoderm formation by rapid cell cycles in an artificially activated insect egg

Oocytes explanted from adult ovaries of the arrhenotokous Hymenopteron Pimpla turionellae remain in an inactive state, because development has not been initiated by mechanical deformation during natural oviposition. However, they could be induced to enter development by injecting cleavage energids into the posterior pole. After lag phases of up to 32 h, the implanted nuclei initiated a normal cleavage process, except that the polarity of its progress was reversed. In other oocytes, the injected energids congregated in a ring-shaped region at the egg surface to form a superficial nuclear front, which slowly advanced towards the anterior egg pole, thereby successively stimulating portions of the quiescent ooplasm to take part in development. Up to 41 rapid cell cycles started from that front, each of them with an anaphase wave running backwards into the region already peripherally occupied by nuclei. Thus, the blastoderm was formed extremely metachronously and by rapid obviously biphasic cell cycles, which never occur at the egg surface during normal cleavage. A germ band, however, was only formed under the following conditions: (1) that cleavage did not follow the nuclear front mode, and (2) that ooplasm from the donor's posterior pole was co-injected with the graft nuclei. We conclude that embryonic differentiation requires some of the events which had been omitted in eggs where development failed, especially the exponential increase of the cell cycle length, and the activity of some posterior factor(s) during egg activation.     

Cell clones and pattern formation: Developmental restrictions in the compound eye ofDrosophila

Summary Five regions of the compound eye have been found to be preferential boundaries for clones of labelledMinute ⁺ cells, and to act restrictively on the growth of cell clones after a given developmental stage. One of these regions is topographically related to the line of pattern inversion existing at the level of the equator. The results of experiments showing independency of origin of restriction lines and line of pattern inversion are reported.     

Global cell sorting in the C. elegans embryo defines a new mechanism for pattern formation

4D microscopic observations of Caenorhabditis elegans development show that the nematode uses an unprecedented strategy for development. The embryo achieves pattern formation by sorting cells, through far-ranging movements, into coherent regions before morphogenesis is initiated. This sorting of cells is coupled to their particular fate. If cell identity is altered by experiment, cells are rerouted to positions appropriate to their new fates even across the whole embryo. This cell behavior defines a new mechanism of pattern formation, a mechanism that is also found in other animals. We call this new mechanism "cell focusing". When the fate of cells is changed, they move to new positions which also affect the shape of the body. Thus, this process is also important for morphogenesis.     

rap gene encodes Fizzy-related protein (Fzr) and regulates cell proliferation and pattern formation in the developing Drosophila eye-antennal disc

The rap (retina aberrant in pattern) gene encodes the Fizzy-related protein (Fzr), which as an activator of the ubiquitin ligase complex; APC/C (anaphase promoting complex/cyclosome) facilitates the cell cycle stage-specific degradation of cyclins. Loss-of-function mutations in rap cause unscheduled accumulation of cyclin B in the developing eye imaginal disc, resulting in additional mitotic cycles and defective patterning of the developing Drosophila eye. Targeted mis-expression of rap/fzr in the eye primordial cells causes precocious cell cycle exit, and smaller primordial eye fields, which either eliminate or drastically reduce the size of the adult eye. Although mitosis is inhibited in the mis-expression animals, cells with abnormally large nuclei form tumor-like structures from continued endoreplication, cell growth and retinal differentiation. Interestingly, overexpression of Rap/Fzr in the eye primordia also increases the size of the antennal primordium resulting in the induction of ectopic antennae. These results suggest that Rap/Fzr plays an essential role in the timely exit of precursor cells from mitotic cycles and indicate that mechanisms that regulate cell cycle exit are critical during pattern formation and morphogenesis.     

Chemical inhibition of cell wall formation and cytokinesis,but not of nuclear division,in protoplasts of Nicotiana tabacum L. cultivated in vitro

The effect of cytochalasin B, colchicine, coumarin and 2,6-dichlorobenzonitrile on cell wall formation and cellular division was studied by light and electron microscopy with tobacco mesophyll protoplasts cultivated in vitro. 2,6-dichlorobenzonitrile was found to be the most effective and reversible inhibitor of cell wall formation. The other inhibitors caused irreversible damage and/or inhibited mitosis. In protoplasts cultivated in the presence of 2,6-dichlorobenzonitrile the total inhibition of cell wall formation had no effect on nuclear division, but cytokinesis was totally inhibited so that multinucleate protoplasts were obtained.Abbreviations DB 2,6-dichlorobenzonitrile=dichlobenil - CB cytochalasin B