共查询到20条相似文献,搜索用时 31 毫秒
1.
Sally A. Holick Michael F. Holick Julia A. MacLaughlin 《Biochemical and biophysical research communications》1980,97(3):1031-1037
The simple three-step preparation of [1β-3H]1α,25-dihydroxyvitamin D3 and [1α-3H]1β,25-dihydroxyvitamin D3 from 1α,25-dihydroxyvitamin D3 is described. In the rat, 1β,25-dihydroxyvitamin D3, when compared with its α-epimer, did not stimulate intestinal calcium transport or bone calcium mobilization at doses 1000-fold higher than the doses of the natural hormone, 1α,25-dihydroxyvitamin D3. 相似文献
2.
Two synthetic routes to 3-deoxy-1α-hydroxyvitamin D3, an analog of 1α,25-dihydroxyvitamin D3, are described. One involved the six-step conversion of 1α,2α-epoxy-6,6-ethylenedioxy-5α-cholestan-3- one to 1α-acetoxycholest-5-ene, whereas, in the second, the same intermediate was prepared from 1α-hydroxycholesterol. Conversion of the Δ5-sterol to the required 5,7-diene was accomplished most efficiently via 7-keto and 7-tosylhydrazone intermediates. Bioassay of 3-deoxy-1α-hydroxyvitamin D3 in the rat establishes that the analog can fulfill all common vitamin D functions including stimulation of intestinal calcium transport, mobilization of calcium and phosphate from bone, stimulation of growth, and calcification of bone. Direct comparison indicates the compound to have to of the activity of 1α-hydroxyvitamin D3, but it acts with a time course indistinguishable from the latter. 相似文献
3.
Susumu Sunaga Noboru Horiuchi Naoyuki Takahashi Kazuo Okuyama Tatsuo Suda 《Biochemical and biophysical research communications》1979,90(3):948-955
Metabolism of 25-hydroxyvitamin D3 (25-OH-D3) in pregnancy was investigated in New Zealand White rabbits fed a rabbit chow. Kidney homogenates from pregnant mothers and fetuses were separately incubated with [3H]-25-OH-D3. The homogenates from fetuses produced significant amounts of D3 [1α,25-(OH)2-D3] from its precursor, while those from mothers predominantly produced [3H]-24,25-dihydroxyvitamin D3 [24,25-(OH)2-D3]. The identity of the radioactive metabolites produced from [3H]-25-OH-D3 was established by periodate cleavage and comigration with synthetic 1α,25-(OH)2-D3 or 24,25-(OH)2-D3 on high pressure liquid chromatography. These results clearly indicate that the fetal kidney is at least one of the sites of 1α,25-(OH)2-D3 synthesis in pregnancy. 相似文献
4.
Shuling Wu Christoph C. Geilen Beate Tebbe Constantin E. Orfanos 《The Journal of nutritional biochemistry》1996,7(12):642-649
Epidermal homeostasis is influenced by a number of hormones and regulative growth factors that are maintained by a tightly regulated balance between cell proliferation, cell differentiation, and cell death. Vitamin D is one of those regulatory factors. It has recently been demonstrated that 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3) takes part in the regulation of the cell cycle by multiple and complex functions. This review discusses 1α,25(OH)2D3 and its analogues in connection with the renewal of epidermal keratinocytes as well as the molecular mechanisms underlying terminal differentiation or rather programmed cell death. Furthermore, interest is focused on the possible clinical application of vitamin D3 analogues. 相似文献
5.
Chisato Miyaura Etsuko Abe Takeo Kuribayashi Hirofumi Tanaka Kunio Konno Yasuho Nishii Tatsuo Suda 《Biochemical and biophysical research communications》1981,102(3):937-943
A human myeloid leukemia cell line [HL-60] could be induced to differentiate into mature myeloid cells by 1α,25-dihydroxyvitamin D3 [1α,25(OH)2D3], the active form of vitamin D3. At 10?10–10?8 M, 1α,25(OH)2D3 suppressed cell growth in a dose-dependent manner and markedly induced phagocytosis and C3 rosette formation. The potency of 1α,25(OH)2D3 in inducing differentiation was nearly equivalent to that of known synthetic inducers such as dimethyl sulfoxide, actinomycin D or a phorbol ester (12-o-tetra-decanoyl-phorbol-13-acetate). These results clearly indicate that 1α,25(OH)2D3, besides its well known biological effect in enhancing intestinal calcium transport and bone mineral mobilization activities, is involved in the cell grwoth and differentiation of HL-60 cells. 相似文献
6.
Anthony W. Norman 《Steroids》1980,36(1):27-39
A heretofore unknown metabolite of vitamin D3 was isolated from the 1α,24,25-trihydroxyvitamln D3 fraction of lipid extracts obtained from plasma of rats which were given intravenous or oral doses of 100 pmol/100 g of either 1α-hydroxyvitamin D3 or 1α, 25-dihydroxy-vitamin D3. Doses of 25–250 pmoles of the new metabolite when given to a vitamin D deficient rat were completely inactive in terms of stimulating the classic vitamin D response of bone calcium mobilization. The nature of the metabolism of 1α-hydroxyvitamin D3 or 1α, 25-dihydroxy-vitamin D3 to the metabolite is not clear at the present time, but it is probable that neither of these steroids undergo side-chain cleavage to yield the new metabolite. 相似文献
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8.
Yasuo Tohira Kiyoshige Ochi Isao Matsunaga Masafumi Fukushima Shigeru Takanashi Kazuhito Hata Chikara Kaneko Tatsuo Suda 《Analytical biochemistry》1977
1α-[2-3H]Hydroxyvitamin D3 was synthesized chemically. The preparation was radiochemically pure and had a high enough specific activity (4.2 Ci/mmol) to permit experiments using 62.5 pmol/rat. This preparation had as much effect as synthetic 1α-hydroxyvitamin D3 in increasing the serum Ca level of vitamin D-deficient rats. 相似文献
9.
G.Christian Friesinger Oswald Oelz Brian J. Sweetman Alan S. Nies Joann L. Data 《Prostaglandins & other lipid mediators》1978,15(6):969-981
Prostaglandin (PG) D2 was biosynthesized by rabbit renal papillae incubates in vitro. Quantification of the renal prostaglandins by gas chromatography-mass spectroscopy demonstrated that the concentration of PGD2 generated by renal papillae was
to
the amount of PGE2 or about 1 μg/g tissue/30 min. Infusion of the sodium salt of PGD2 into the renal artery of the dog produced a dose related increase in renal blood flow and urine flow, free water clearance, sodium excretion and potassium excretion without changes in systemic hemodynamics. At low doses PGD2 increased renal blood flow to all cortical zones. Higher concentrations of PGD2 produced a shift in the intrarenal distribution of blood flow toward the juxtamedullary nephrons. 相似文献
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11.
目的:探讨卡马西平联合左甲状腺素对甲状腺功能减退症患者血清胰岛素样生长因子结合蛋白-3(IGFBP-3)、25羟维生素D3(25(OH)D_3)的影响。方法:选择2017年5月~2018年6月本院接诊的103例甲状腺功能减退症患者进行研究,通过随机数表法分为观察组52例和对照组51例,对照组给予左甲状腺素口服治疗,观察组在对照组基础上联合卡马西平口服治疗,两组患者均连续治疗8周。比较两组临床疗效、治疗前后甲状功能、血清IGFBP-3和25(OH)D_3的变化情况及不良反应。结果:治疗后,观察组患者的临床疗效为94.23%,明显高于对照组患者的84.31%(P0.05);观察组血清FT3、TSH均明显比对照组低,FT4明显高于对照组(P0.05);两组患者治疗后IGFBP-3和25(OH)D_3的指标水平均高于治疗前,且观察组结果高于对照组(P0.05);观察组不良反应总发生率为5.76%,低于对照组的13.73%,两组不良反应总发生率差异无统计学意义(P0.05)。结论:卡马西平联合左甲状腺素对甲状腺功能减退症患者临床效果显著,可有效改善患者甲状腺功能,调节患者血清IGFBP-3和25(OH)D_3的表达水平,且不良反应少、安全性高,值得推广。 相似文献
12.
Naoko Urushino Shinichi Ikushiro Miho Ohta 《Biochemical and biophysical research communications》2009,384(2):144-1661
The metabolism of 1α,25-dihydroxyvitamin D2 (1α,25(OH)2D2) by human CYP24A1 was examined using the recombinant enzyme expressed in Escherichia coli cells. HPLC analysis revealed that human CYP24A1 produces at least 10 metabolites, while rat CYP24A1 produces only three metabolites, indicating a remarkable species-based difference in the CYP24A1-dependent metabolism of 1α,25(OH)2D2 between humans and rats. LC-MS analysis and periodate treatment of the metabolites strongly suggest that human CYP24A1 converts 1α,25(OH)2D2 to 1α,24,25,26(OH)4D2, 1α,24,25,28(OH)4D2, and 24-oxo-25,26,27-trinor-1α(OH)D2 via 1α,24,25(OH)3D2. These results indicate that human CYP24A1 catalyzes the C24-C25 bond cleavage of 1α,24,25(OH)2D2, which is quite effective in the inactivation of the active form of vitamin D2. The combination of hydroxylation at multiple sites and C-C bond cleavage could form a large number of metabolites. Our findings appear to be useful to predict the metabolism of vitamin D2 and its analogs in the human body. 相似文献
13.
研究以黄颡鱼(Pelteobagrus fulvidraco)头肾巨噬细胞为研究对象,通过细菌脂多糖(LPS)和环磷酸腺苷(cAMP)分别诱导M1型和M2型极化,200 pmol/L维生素D3孵育后对其形态学特征、生物学功能及极化相关基因的表达进行分析鉴定来确定维生素D3在巨噬细胞极化中的调节作用。结果表明,维生素D3能降低诱导后M1型和M2型巨噬细胞的死亡率,并增强巨噬细胞的吞噬活性。在M1型巨噬细胞中维生素D3能够抑制活性氧(ROS)和炎症介质一氧化氮(NO)的产生,降低超氧阴离子自由基的活力,白介素1β(IL-1β)和肿瘤坏死因子-α(TNF-α)的表达水平显著降低(P<0.05);在M2型细胞中能够增加精氨酸酶的活性,显著增加白介素10(IL-10)和转化生长因子(TGF-β)的表达水平(P<0.05),最终抑制巨噬细胞向M1表型极化,促进巨噬细胞向M2表型极化,发挥抗炎作用;黄颡鱼头肾巨噬细胞中Nos-2和Arg-2分别是M1和M2巨噬细胞的生物标记基因。研究结果为进一步研究鱼... 相似文献
14.
Toshimasa Shinki Yoshiko Shiina Naoyuki Takahashi Yoshikuni Tanioka Hitoshi Koizumi Tatsuo Suda 《Biochemical and biophysical research communications》1983,114(2):452-457
Compared to most mammals, the marmoset, a new world monkey, requires particularly large amounts of vitamin D to maintain normal growth. We compared serum concentrations of vitamin D metabolites in marmosets with rhesus monkeys and humans. The circulating levels of 1α,25-dihydroxyvitamin D3 [1α,25(OH)2D3] in marmosets were 4 to 10 times higher than those in rhesus monkeys and humans. But none of the marmosets exhibited hypercalcemia. In two marmosets which had suffered bone fractures, the 1α,25-(OH)2D3 levels were particularly elevated. These results suggest that the marmoset has an end-organ resistance to 1α,25(OH)2D3. 相似文献
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16.
Jonas Abdel-Khalik Peter J. Crick Graham D. Carter Hugh L. Makin Yuqin Wang William J. Griffiths 《Biochemical and biophysical research communications》2014
The total serum concentration of 25-hydroxyvitamins D (25-hydroxyvitamin D3 and 25-hydroxyvitamin D2) is currently used as an indicator of vitamins D status. Vitamins D insufficiency is claimed to be associated with multiple diseases, thus accurate and precise reference methods for the quantification of 25-hydroxyvitamins D are needed. Here we present a novel enzyme-assisted derivatisation method for the analysis of vitamins D metabolites in adult serum utilising 25-[26,26,26,27,27,27-2H6]hydroxyvitamin D3 as the internal standard. Extraction of 25-hydroxyvitamins D from serum is performed with acetonitrile, which is shown to be more efficient than ethanol. Cholesterol oxidase is used to oxidize the 3β-hydroxy group in the vitamins D metabolites followed by derivatisation of the newly formed 3-oxo group with Girard P reagent. 17β-Hydroxysteroid dehydrogenase type 10 is shown to oxidize selectively the 3α-hydroxy group in the 3α-hydroxy epimer of 25-hydroxyvitamin D3. Quantification is achieved by isotope-dilution liquid chromatography–tandem mass spectrometry. Recovery experiments for 25-hydroxyvitamin D3 performed on adult human serum give recovery of 102–106%. Furthermore in addition to 25-hydroxyvitamin D3, 24,25-dihydroxyvitamin D3 and other uncharacterised dihydroxy metabolites, were detected in adult human serum. 相似文献
17.
1,25D3 is critical for the maintenance of normal reproduction since reduced fertility is observed in male rats on a vitamin D-deficient diet. Vitamin D-deficient male rats have incomplete spermatogenesis and degenerative testicular changes. In the present study we have examined the ionic involvement and intracellular messengers of the stimulatory effect of 1,25D3 on amino acid accumulation in immature rat testis. 1,25D3 stimulates amino acid accumulation from 10−12 to 10−6 M by increasing the slope to reach a maximum value at 10−10 M, as compared to the control group. No effect was observed at a lower dose (10−13 M). Time-course showed an increase on amino acid accumulation after 15, 30, and 60 min of incubation with 1,25D3 (10−10 M). 1,25D3 stimulated amino acid accumulation in 11-day-old rat testis but not in testis that were 20 days old. Cycloheximide totally blocked the 1,25D3 action on amino acid accumulation. Furthermore, a localized elevation of cAMP increased the stimulatory effect of 1,25D3 and the blockage of PKA nullified the action of the hormone. In addition, 1,25D3 action on amino acid accumulation was also mediated by ionic pathways, since verapamil and apamine diminished the hormone effect. The stimulatory effect of 1,25D3 on amino acid accumulation is age-dependent and specific to this steroidal hormone since testosterone was not able to change amino acid accumulation in both ages studied. This study provides evidence for a dual effect for 1,25D3, pointing to a genomic effect that can be triggered by PKA, as well as to a rapid response involving Ca2+/K+ channels on the plasma membrane. 相似文献
18.
The reactions of the triangulo-cluster [Pt3(μ-CO)3(PtBu3)3] with activated olefins and alkynes have been examined under various conditions. At low temperature, cluster fragmentation occurs yielding the Pt(0) complexes [Pt(CO)(PtBu3)(olefin)] (olefin = maleic anhydride and maleimide), while di(tert-butyl)acetylenedicarboxilate reacts quantitatively giving the dinuclear Pt(0) complex [Pt2(CO)2(PtBu3)2(μ-η2:η2-tBuO2CCCCO2tBu)]. At higher temperature and in the presence of alkyne in large excess, the latter dimer converts quantitatively to the monomers [Pt(CO)(PtBu3)(alkyne)] (alkyne = CF3CCCF3 and tBuO2CCCCO2tBu). The stereochemistry of these complexes has been established by NMR and IR measurements. The structure of [Pt(CO)(PtBu3)(CF3CCCF3)] was confirmed by X-ray diffraction analysis. 相似文献
19.
摘要 目的:探讨支气管哮喘(简称"哮喘")患儿血清半乳糖凝集素3(Gal-3)、类胰蛋白酶、25-羟维生素D3 [25(OH)D3]与肺功能和生活质量的相关性。方法:选取我院2018年2月~2021年3月收治的136例哮喘患儿为研究组,行肺功能检测,将其按照病情严重程度分作轻度组63例、中度组41例及重度组32例。另取同期健康体检儿童40例作为对照组。检测并比较各组血清Gal-3、类胰蛋白酶、25(OH)D3水平。对比各组哮喘患儿肺功能指标,以中文版儿科哮喘生命质量调查问卷(PAQLQ)对哮喘患儿进行生活质量评估。并以Pearson相关性分析哮喘患儿血清Gal-3、类胰蛋白酶、25(OH)D3水平与肺功能和生活质量的关系。结果:研究组的血清Gal-3、类胰蛋白酶水平均高于对照组,且轻度组、中度组、重度组逐渐升高;血清25(OH)D3水平均低于对照组,轻度组、中度组、重度组逐渐下降(P<0.05)。中度组及重度组第1秒用力呼气容积占预计值百分比(FEV1%pred)、呼吸峰流速占预计值百分比(PEF%pred)、第1秒用力呼气容积/用力肺活量(FEV1/FVC)以及PAQLQ各项评分均低于轻度组,且重度组低于中度组,差异均有统计学意义(P<0.05)。经Pearson相关性分析可得:哮喘患儿血清Gal-3、类胰蛋白酶水平与各项肺功能指标以及PAQLQ各项评分均呈负相关,而血清25(OH)D3水平与与各项肺功能指标以及PAQLQ各项评分均呈正相关(均P<0.05)。结论:血清Gal-3、类胰蛋白酶、25(OH)D3水平与哮喘患儿的肺功能以及生活质量密切相关,可能成为评估其病情及生活质量的可靠指标。 相似文献
20.
Cheryl M. Heesch Guillermo Valenzuela Barrie J. Hodgson 《Prostaglandins & other lipid mediators》1977,14(2):279-282
The effects of PGD2, PGF2α and PGE1 were studied on the circular muscle of post-ovulatory rabbit oviducts . PGE1 inhibited spontaneous contractile activity. Lower concentrations of PGD2 and PGF2α were stimulatory and higher concentrations were inhibitory. Since PGD2 may be produced in the oviduct, any hypothesis concerning the role of prostaglandins in the control of oviductal motility and ovum transport should include PGD2 as well as PGFs and PGEs. 相似文献