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Antagonism between apolipoprotein AI regulatory protein 1, Ear3/COUP-TF, and hepatocyte nuclear factor 4 modulates apolipoprotein CIII gene expression in liver and intestinal cells. 总被引:27,自引:13,他引:14
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M Mietus-Snyder F M Sladek G S Ginsburg C F Kuo J A Ladias J E Darnell Jr S K Karathanasis 《Molecular and cellular biology》1992,12(4):1708-1718
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Mechanism of a transcriptional cross talk between transforming growth factor-beta-regulated Smad3 and Smad4 proteins and orphan nuclear receptor hepatocyte nuclear factor-4 总被引:5,自引:0,他引:5
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Chou WC Prokova V Shiraishi K Valcourt U Moustakas A Hadzopoulou-Cladaras M Zannis VI Kardassis D 《Molecular biology of the cell》2003,14(3):1279-1294
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J A Ladias M Hadzopoulou-Cladaras D Kardassis P Cardot J Cheng V Zannis C Cladaras 《The Journal of biological chemistry》1992,267(22):15849-15860
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Hierarchy and positive/negative interplays of the hepatocyte nuclear factors HNF-1, -3 and -4 in the liver-specific enhancer for the human alpha-1-microglobulin/bikunin precursor. 总被引:2,自引:0,他引:2
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Alpha-1-microglobulin and bikunin are two plasma glycoproteins encoded by an alpha-1-microglobulin/bikunin precursor (AMBP) gene. The strict liver-specific expression of the AMBP gene is controlled by a potent enhancer made of six clustered boxes numbered 1-6 that have been reported to be proven or potential binding sites for the hepatocyte-enriched nuclear factors HNF-1, -4, -3, -1, -3, -4, respectively. In the present study, electromobility shift assays of wild-type or mutated probes demonstrated that the boxes 1-5 have a binding capacity for their cognate HNF protein. Box 5 is also a target for another, as yet unidentified, factor. A functional analysis of the wild-type or mutated enhancer, driving its homologous promoter and a reporter CAT gene in the HepG2 hepatoma cell line, demonstrated that all six boxes participate in the enhancer activity, with the primary influence of box 4 (HNF-1) and box 2 (HNF-4). A similar analysis in the HNF-free CHO cell line co-transfected with one or several HNF factors further demonstrated various interplays between boxes: box 3 (HNF-3 alpha and beta) has a negative influence over the major HNF-4 box 2 as well as a positive influence over the major HNF-1 box 4. 相似文献